RADHWANE SAIDI - Academia.edu (original) (raw)

Papers by RADHWANE SAIDI

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, May 29, 2023

The present study aims to evaluate the prevalence and some associated risk factors of Fasciola he... more The present study aims to evaluate the prevalence and some associated risk factors of Fasciola hepatica in sheep in the Algerian agro-pastoral region, Djelfa. For this, a total of 217 blood samples were taken from sheep (100 females and 117 males) aged on average ten months in two slaughterhouses in an Algerian agro-pastoral region, Djelfa during the period from January to October 2021 and analyzed by the ELISA method (BIOK 211-Monoscreen AbELISA Fasciola hepatica test) at the regional veterinary laboratory of Laghouat. The collected sera were numbered and stored at-20°C until analysis. Technical sheets were filled out with each sampled animal, including information on age, sex, locality and season. The results showed that the rate of positive seroprevalence of Fasciola hepatica was 0.46% in sheep. This positivity rate was found in April in a 2-year-old female. At the same time, two doubtful cases (0.92%) were identified, one in a five-year-old female during the month of August and the other in a six-month-old male during the month of September. These results indicate the possibility of fasciolosis infestation in sheep farms in the Djelfa region and the zoonotic risk that this represents, as well as the economic losses that result from it.

Veterinary World

Background and Aim: An ethnobotanical survey was carried out among the inhabitants of the Aflou r... more Background and Aim: An ethnobotanical survey was carried out among the inhabitants of the Aflou region of Laghouat (Southern Algeria). This study was considered as a first step toward the identification of new bioactive antiparasitic molecules. The preservation and documentation of this traditional knowledge will ensure its continuity and transmission from one generation to another, especially because of the emergence of resistant parasites and the lack of references caused by the lack of work in this area; therefore, we intended to inventory and collect the maximum amount of information on medicinal plants that are traditionally used by the local population as antiparasitic in humans and animals (small ruminants, cattle, and livestock). Materials and Methods: The information was collected using open interviews; the ethnobotanical survey was carried out in the area mentioned above from April to July 2021 using a semi-structured questionnaire and a global sample of 200 respondents. T...

The objective of this study was to evaluate a test for the rapid diagnosis of mastitis in cattle ... more The objective of this study was to evaluate a test for the rapid diagnosis of mastitis in cattle farms in the central region of Algeria. The study was conducted on 100 lactating cows belonging to 15 farms. The test evaluated was the speed® mam color. These cows were screened for mastitis by the California Mastitis Test (CMT).The positive samples at the CMT were subjected to a bacteriological analysis, to identify the pathogenic germs. The classic bacteriological analysis and the Speed® Mam Color were used for the identification of pathogenic bacteria responsible for bovine mastitis and the achievement of a rapid antibiogram.With CMT, the prevalence of subclinical mastitis was evaluated at 25% of cows tested and bacteriological culture using a rapid test: speed® mam color was positive in 96% [24/25] of cows positive at WCL. However, classical bacteriological analysis of CMT-positive samples showed a positivity of 100% [25/25]. This result showed a very good correlation [96%] between ...

Contribution à l’évaluation de l’activité anticonvulsivante de la partie aérienne de Lavandula stoechas L. chez la souris

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2015

The study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrob... more The study aimed to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of 21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12 antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in the Mueller-Hinton agar, and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%), and erythromycin (76.19%) but, no resistance in all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to X...

Acta Scientiarum Technology

The massive use of antibiotics is among the leading causes of bacterial resistance. The objective... more The massive use of antibiotics is among the leading causes of bacterial resistance. The objective of this study is to investigate whether the sewage of the Maringá Regional University Hospital (HUM) has pathogenic bacteria resistant to antibiotics. The samples were collected at two sites, one from the entire hospital and the other from the blood bank. 100 mL were inserted in dilutions of 10-2, 10-3 and 10-4 in cultures Mac Conkey Agar, Salmonella-Shigella Agar, Mannitol Salt Agar and Sabouraud Dextrose Agar. The samples were subjected to bacterial identification and antibiogram. The identification was based on bacterioscopy by Gram staining, and biochemical evidence. The antibiograms were made in accordance with the disk diffusion methodology in Mueller-Hinton agar, and 12 antibiotics were used in these tests. They were isolated and 39 samples were identified; of these, 18 species showed resistance and moderate resistance to at least one antibiotic. Bacteria isolated and identified ...

Effectiveness of Some Sanitary Measures on the Survival of Mastitis: The Point on Pre and Post Soak

Veterinarska stanica, 2021

This study aimed to identify gastrointestinal parasites in camels (Camelus dromaderius) in the La... more This study aimed to identify gastrointestinal parasites in camels (Camelus dromaderius) in the Laghouat region (southern Algeria). The study was carried out over a 5-month period on a total of 100 dromedaries. Dung samples were analysed using different methods such as flotation, sedimentation, and Ziehel-Neelsen staining for research of the cryptosporidiosis. Data showed an overall infestation rate of 78%, with the presence of the following parasites: Cryptosporidium sp. (60%), Nematodes: Nematodirussp (23%), Strongyloides sp. (4%), Marshallagia sp. (2%), and Cooperiasp (3%), different protozoaires: Eimeria sp. (20%), Neobalantidium sp. (2%), and Balantidium coli, cestodes (6%), Moniezia sp. (3%), Multicips sp. (2%), Diphillobothrium sp. (1%), and trematodes: Fasciola hépatica (4%) and Paramphistomum sp (1%). The results showed a significant influence of study site on the parasitic infestation rate (P=0.039). Other factors (sex, age and clinical aspect) had no significant influence....

Mastitis is the most costly disease in dairy industry. Staphylococcus spp. is the most frequently... more Mastitis is the most costly disease in dairy industry. Staphylococcus spp. is the most frequently isolated microorganisms and Staphylococcus aureus is one of the most important contagious mastitis agents in dairy cattle. The aim of this study is developing a duplex PCR technique for detection of Staphylococcus spp. and S. aureus from culture and milk samples. S. aureus (ATCC 25923) and S. epidermidis (ATCC 12228) DNAs were used as positive control. For the purpose of testing the developed technique, one coagulase-negative Staphylococcus and one S. aureus positive milk sample was used as a clinical sample that they were sent by Veterinary Practitioners. In this study, a duplex and rapid PCR protocol is developed for detecting and separating these organisms from culture and from milk samples. This procedure can be used as an alternative, reliable and fast detection and identification method for Staphylococcus spp. and S. aureus in few hours for deciding treating or culling the cows in...

Sığır Mastitis Süt Örneklerinden Stafilokok Türlerinin ve S. aureus'un Teşhisine Yönelik İkili bir PZR Tekniği

The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 i... more The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 individuals (sheep, goats, cattle and dogs) in southern Algeria was studied from March 2019 to February 2020 during monthly visits. A total of 2544 ticks were collected by examining the entire body of the animal. Two tick species were identified: Hyalomma dromedarii (2430 ticks) and Rhipicephalus sanguineus sensu lato (114 ticks). The first species had annual activity and consisted only of adults, while the second species had spring and summer activity and contained both adults and nymphs. At the annual level, the prevalence of infestation, abundance, and intensity were 38.60%, 44.63 and 115.64 ticks per animal, respectively. At the monthly level there were speciesspecific fluctuations, with a peak in activity in September.

Contribution to studying ecto and mesoparasites in goats in Southern Algeria

Veterinaria, 2020

The present work is aimed at conducting a screening survey to determine the parasites infesting g... more The present work is aimed at conducting a screening survey to determine the parasites infesting goats in Algeria evaluation of their prevalence, and studying the influence of some risk factors on the incidence of these parasites. For this reason, the fact sheets on 100 animals belonging to 10 visited farmers were filled, macroscopic research of ectoparasites was completed and the faecal samples were taken. Different methods such as direct examination, flotation method, the method of Ritchie, Lugol staining technique and modified Ziehl - Neelsen staining were used to identify the parasite eggs. The results showed a total prevalence of vermin infestation of 97%. The observed mesoparasites were: Eimeria spp. (89%), Cryptosporidium spp. (70%), Ostertagia spp. (19%), Nematodirus spp. (14%), Skrjabinema spp. (10%) and Strongyloides spp. (8%). Ectoparasites were detected in 3% of examined goats and they were represented by two species of lice: Linognathus africanus (3%) and Damalinia capra...

Zootechnical and Sanitary Survey of Broiler Reproducers in the West of Algeria

This study aimed to assess the mastery level of poultry farming for broiler reproducers, through ... more This study aimed to assess the mastery level of poultry farming for broiler reproducers, through the evaluation of the sanitary and zootechnical performances obtained at the level of Commercial Poultry Farms in Tiaret region (the west of Algeria). The results obtained showed that the zootechnical parameters didn’t follow the entirely international standards. Hygrometry and temperature were not controlled. The average mortality rate of males was higher (42.92%) than that of females (8.84%), (P<0.05). Concerning production, the spawning performance was generally within the norms. The onset of laying was around the 24th week and the peak was late; it happened around the 30th week in the farm. The temperature of egg storage (15-18°C) was timidly respected as well as the duration of storage (2 weeks). According to this study devoted to the monitoring of breeding hens, the results showed a variable level of compliance with standards as well as production.

Prevention et traitement des parasitoses

Dans le monde, on compte plus de 4 milliards de bovins, de porcs, d’ovins, de caprins, de chevaux... more Dans le monde, on compte plus de 4 milliards de bovins, de porcs, d’ovins, de caprins, de chevaux, de buffles et de chameaux. En revanche, on connait mal les problemes de sante humaine et animale. De plus, la prevention des problemes de sante humaine et celle des animaux passera par une bonne comprehension et un traitement approprie suite a un bon diagnostic. Le present ouvrage a pour principal objectif de resumer le maximum d'informations sur les moyens curatifs et preventifs preconises en cas d'une parasitose.

Study of the Antibacterial Activity of Essential Oils of Certain Plants on Isolated Bacteria of Mammary Milk in Algeria

The infection or inflammation of the mammary gland represents a dominant pathology in dairy cattl... more The infection or inflammation of the mammary gland represents a dominant pathology in dairy cattle. The aim of this work was to study in vitro the antimicrobial activity of essential oils of some plants on bacteria which were isolated from mammary milk. The essential oils of Thymus vulgaris, Rosmarinus officinalis, Artimisia herba alba and Myrtus communis were obtained by hydro distillation of the aerial part. They were tested on strains of staphylococci that were multi-resistant to antibiotics which are isolated from mammary milk. The essential oils contents are between a low value of 0.3% for Myrtus communis and a relatively high value of 2.5% for Thymus vulgaris. The different essential oils tested revealed interesting antimicrobial activities. The strongest antibacterial activity was observed for the essential oil of Thymus vulgaris (9-52 mm), while the oils of Rosmarinus oficinalis, Artimisia herba alba and Myrtus communis showed moderate antimicrobial activities (9-19.5 mm). W...

This study was carried out in order to identify the different parasites (ecto and mesoparasites) ... more This study was carried out in order to identify the different parasites (ecto and mesoparasites) infesting the tortoise Testudo graeca graeca. For this, a population of 24 tortoises was studied from mid-March to May 2018, in the region of Aflou-Laghouat, within two sites: Djellel and Oued Sebgag. The turtles were captured at the time of their activity; sex, age, body weight, total length of the shell and body height were determined. A careful search for parasites has been carried out using various recommended techniques. A total of 126 Hyalomma aegyptium ticks were identified, with an overall prevalence of 71%, an average intensity of 7.4 and an abundance of 5 ticks per individual. The stool examination identified six mesoparasites, namely: Angusticaecum holopterum, Enterobius vermicularis, Strongylus sp, Nematodirus sp, Trichostrongylus sp, Fasciola hepatica. The sex of the host showed a significant influence on the parasitic infestation: the females were four times more infested t...

TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES

The Akabane (AKA) and Bluetongue (BT) viruses are nonzoonotic and mosquito-borne viruses carried ... more The Akabane (AKA) and Bluetongue (BT) viruses are nonzoonotic and mosquito-borne viruses carried by livestock and wild animals. They cause significant economic impacts as a result of the reproductive disorders they produce, such as early births, abortions, stillbirths, and malformations, especially in ruminants (cattle, sheep, and goats). AKAV belongs to the genus Orthobunyavirus in the family Peribunyaviridae as classified by the International Committee on Taxonomy of Viruses [1]. AKAV infection is widely reported throughout the world at present, including Australia, Southeast Asia, East Asia, and the Middle East [2-8], although AKAV was first detected in Japan, where it is called enzootic bovine arthrogryposis hydranencephaly. Moreover, antibodies to AKAV have been detected in many host species, such as cattle, horses, donkeys, sheep, goats, pigs, camels, and buffalo [9]. BTV belongs to the genus Orbivirus in the family Reoviridae [10,11]. In cattle and goats, BTV infection is seen mostly without clinical symptoms; however, the disease may cause severe clinical signs in sheep and deer that are characterized by hemorrhagic fever and death [12,13]. BTV occurs more widely in a band between latitudes of 40°N and 35°S worldwide [11], including sub-Saharan and Mediterranean countries [10]; therefore, hematophagous insects have a wide distribution in Algeria based upon their settlement-suitable climatic zones.

African Journal of Microbiology Research, Feb 26, 2013

Mastitis is the most complex and costly disease of dairy cows occurring throughout the world. In ... more Mastitis is the most complex and costly disease of dairy cows occurring throughout the world. In Algeria, the disease is not well investigated. A cross-sectional study to elucidate its magnitude, distribution, bacterial causes, and to investigate the reliability of a test for early diagnosis of mastitis in cattle in Blida and Ain Defla governorates, central region of Algeria, was carried out from May to December 2011 in a total of 108 milking cows using California mastitis test, clinical inspection of udder and bacteriological analysis. After conducting california mastitis test (CMT) in farms, quarter-based milk samples were collected from 108 cows. Also, 50 positive samples with CMT were collected for bacterial culture. Based on CMT, 29.20% of quarters and 29.62% of cows had subclinical mastitis; the sensitivity of CMT to infections with all bacteria was 96%. Staphylococcus aureus were the most common pathogens (40%). Based on the results of the current study, CMT has very acceptable sensitivity in diagnosis.

Evaluation d'un test de dépistage précoce des mammites subcliniques des vaches

Revue d'élevage et de médecine …, 2010

L’objectif de cette étude a été d’évaluer un test pour le diagnostic des mammites dans les élevag... more L’objectif de cette étude a été d’évaluer un test pour le diagnostic des mammites dans les élevages bovins de la région Centre de l’Algérie. L’étude a été réalisée sur 100 vaches en lactation appartenant à 15 élevages. Le test évalué a été le California Mastitis Test (CMT) dont la positivité a été indiquée par la modification de consistance du mélange lait et réactif sur une cupule à fond noir. Une analyse bactériologique a été réalisée sur tous les échantillons de lait positifs, afin de confirmer la présence d’une infection et d’identifier les pathogènes responsables. Le CMT a été positif chez 25 p. 100 des vaches. La prévalence a été deux fois plus élevée chez les vaches produisant plus de dix litres de lait. L’analyse bactériologique a montré que 96 p. 100 des échantillons de lait positifs contenaient des bactéries pathogènes, parmi lesquelles les staphylocoques ont été les plus fréquemment isolés. Ces résultats ont montré une très forte corrélation entre le CMT et la bactériologie. Ainsi, le CMT semble être un outil avantageux à développer, à petite ou à grande échelle, pour un dépistage systématique et régulier dans un programme intégré de lutte contre les mammites.

Antibiotic susceptibility of Enterobacteriaceae isolated from patients in Northern Tanzania