RAM NARESH PANDEY - Academia.edu (original) (raw)
Papers by RAM NARESH PANDEY
Journal of Essential Oil Research, 2003
Twenty one accessions of vetiver (Vetiveria zizanioides (L.) Nash, sterile, oil type) and Khus (V... more Twenty one accessions of vetiver (Vetiveria zizanioides (L.) Nash, sterile, oil type) and Khus (V. zizanioides, fertile, non-oil type) were analyzed by the use of random amplified polymorphic DNAs (RAPDs). Nineteen of the accessions clustered strongly around the cultigen, Sunshine. Three accessions, Khus, Northern India, Kassel, Germany, and Guang Dong, China clustered loosely and were not closely related to the sterile oil producing vetivers. Thirteen of the vetiver accessions were grown in test plots in Florida, USA, Nepal and Portugal. The largest growth was recorded in Nepal, followed closely by Florida and by the cooler, Mediterranean site in Portugal. No single genotype (DNA cultivar) grew best in every plot. Oil yields (% oil/dry wt.) were highest in Nepal and Portugal. Oil yields ranged from 0.29% to 9.61%. Essential oil production (g/plant) was highest in Nepal and Florida and ranged from 0.02 to 4.17 (g/plant). Analyses of variation among the major compounds is discussed.
Biochemical Systematics and Ecology, 2003
Juniperus communis L. var. communis, J. c. var. depressa Pursh, J. c. var. hemispherica J. & C. P... more Juniperus communis L. var. communis, J. c. var. depressa Pursh, J. c. var. hemispherica J. & C. Presl, J. communis var. megistocarpa Fern. & St. John, J. c. var. nipponica (Maxim.) Wils., J. c. var. oblonga hort. ex Loudon and J. c. var. saxatilis Pall. were sampled and DNA fingerprinting (RAPDs, Random Amplified Polymorphic DNAs) was performed.
Plant Molecular Biology Reporter, 1996
A survey of the inhibition of the amplification of spinach DNA by various plant polysaccharides r... more A survey of the inhibition of the amplification of spinach DNA by various plant polysaccharides revealed that neutral polysaccharides (arabinogalactan, dextran, gum guar, gum locust bean, inulin, mannan, and starch) were not inhibitory. In contrast, the acidic polysaccharides (carrageenan, dextran sulfate, gum ghatti, gum karaya, pectin, and xylan)were inhibitory. In the process of preparing random amplified polymorphic DNAs (RAPDs), the loss of large DNA bands appears to be an indicator that the fingerprint pattern has been affected by polysaccharides. The addition of various concentrations of Tween 20, DMSO, or PEG 400 to the PCR reaction mixture resulted in partial restoration of amplification of RAPDs for the acidic polysaccharides. The most effective way to eliminate the effects of polysaccharide inhibition was by diluting the DNA extracts, and thereby diluting the polysaccharide inhibitors.
Biochemical Systematics and Ecology, 2005
Analyses of individuals classically treated as Juniperus oxycedrus L. var. oxycedrus from Morocco... more Analyses of individuals classically treated as Juniperus oxycedrus L. var. oxycedrus from Morocco, Portugal, Spain, France, Italy, Greece and Turkey, using DNA sequencing of nrDNA (ITS 1, 5.8S, ITS 2) plus RAPDs, leaf terpenoids and morphology revealed that two cryptic, genetically distinct but morphologically almost identical species are present. These species, J. oxycedrus L. var. oxycedrus and Juniperus deltoides R.P. Adams, are about as different from each other as Juniperus navicularis and Juniperus macrocarpa are from J. oxycedrus var. oxycedrus. Examination of herbarium specimens revealed that the two species are largely allopatric with J. deltoides occurring from Italy eastward through Turkey into the Caucasus Mts. and Iran. J. oxycedrus var. oxycedrus appears to be largely concentrated west of Italy (France, Spain, Portugal, Morocco). Cryptic speciation is discussed.
Biochemical Systematics and Ecology, 2003
Twelve populations of Juniperus communis L. were sampled from throughout the arctic, worldwide an... more Twelve populations of Juniperus communis L. were sampled from throughout the arctic, worldwide and DNA fingerprinting (RAPDs, Random Amplified Polymorphic DNAs) was performed. Based on 152 RAPD bands, all of the populations (J. communis var. depressa Pursh and J. communis var. megistocarpa Fern. and St. John) from the western hemisphere formed one group and all of the populations of the eastern hemisphere (including Greenland and Iceland), formed another group that included J. communis var. communis and J. communis var. saxatilis Pall., except for the Kamchatka population that was quite dissimilar to any population examined. Most likely, the current sites of all of the populations were covered with ice or otherwise inhospitable, up to or during the late Pleistocene (ca. 12,000 BP). Therefore, these populations are recent in origin. The path of re-colonization appears to have been northward in North America. Greenland appears to have been colonized from Iceland plants, which in turn came from northern Europe. The Kamchatka population seems likely to have come from Japan.
Biochemical Systematics and Ecology, 2003
Random amplified polymorphic DNAs (RAPDs) data were analyzed from all the taxa of Cupressus from ... more Random amplified polymorphic DNAs (RAPDs) data were analyzed from all the taxa of Cupressus from the western hemisphere. Populations of Cupressus from Arizona and Texas, USA, were found to cluster in the two groups delimited by Wolf (1948): C. arizonica and C. glabra. These data suggest that these taxa might be better recognized at the specific level as per Wolf (1948), rather than at the varietal level or not at all .
Biochemical Systematics and Ecology, 2003
Twelve individual genotypes selected from Juniperus populations, varieties and species were analy... more Twelve individual genotypes selected from Juniperus populations, varieties and species were analyzed using ITS sequences, RAPDs, ISSRs, and leaf volatile terpenoids. These four data sets, all analyzed in the same manner, illustrated that these data sets can be used at different organizational levels: specific, inter-specific and intraspecific. Similarity matrices for the ITS, RAPD, and ISSR data sets were highly correlated (r ranged from 0.83 to 0.95). In contrast, the terpenoid matrix had a low correlation with the other data matrices (r ranged from Ϫ0.04 to 0.38). Because these 12 genotypes were taken from several species, the terpenoids, having been shown to most useful at the infra-specific level, were not concordant at this taxonomic level. The high correlation between DNA markers and ITS sequence data implies that these data are measuring concordant patterns among these genotypes.
Biochemical Systematics and Ecology, 2003
Random Amplified Polymorphic DNAs (RAPDs) data were analyzed from eighteen taxa of
Journal of Essential Oil Research, 2003
Samples of Juniperus thurifera L. were collected from the Atlas Mts., Morocco, northern and south... more Samples of Juniperus thurifera L. were collected from the Atlas Mts., Morocco, northern and southern Spain, the Pyrenees, France, Fench Alps and Corse Isaland, France. The leaf oils were analyzed and found to be polymorphic for several major compounds (sabinene, limonene, linalool, piperitone, linalyl acetate and sesquiterpenes). In general, the Moroccan trees were higher in sabinene, γ-terpinene, cis-sabinene hydrate and terpinen-4-ol, but lower in limonene, δ-2-carene, and piperitone than trees from Europe. Analysis based on Random Amplified Polymorphic DNAs (RAPDs) for the aforementioned population plus J. foetidissima (as an outgroup), revealed that the Moroccan J. thurifera populations were most similar to plants from southern Spain, then to populations from France. Although the trees generally clustered by populations, there appear to be some differentiation in the RAPDs between the European J. thurifera populations and the Moroccan populations. Combining previous studies on seeds per cone, proanthrocyanidins, and the current report on the leaf essential oils and RAPDs, there is some support for the continued recognition of J. thurifera var. africana Maire [syn.: J. Africana (Maire); J. thurifera ssp. africana (Maire) Gauquelin, Hassani et Lebreton] in Algeria and Morocco.
Biochemical Systematics and Ecology, 2002
DNA was examined by RAPD banding for Junipers chinensis, J.c. var. sargentii, J.c. var. tsukusien... more DNA was examined by RAPD banding for Junipers chinensis, J.c. var. sargentii, J.c. var. tsukusiensis, J. communis, J.c. var. nipponica, J.c. var. saxatilis, J. conferta, J. formosana, J. procumbens, J. rigida, J. taxifolia and J.t. var. lutchuensis. The DNA data readily separated junipers of section Sabina from section Juniperus. J.c. var. tsukusiensis from Taiwan was found to be sufficiently different from J.c. var. tsukusiensis (Yakushima) to warrant the recognition of a new variety: J. chinensis var. taiwanensis R.P. Adams and C-F. Hsieh nov. var. Juniperus formosana from mainland China was found to be different from J. formosana from Taiwan and a new variety is recognized: J. formosana var. mairei (Lemee and Lev.) R.P. Adams and C-F. Hsieh comb. nov. Juniperus communis var. nipponica was found to be very distinct from J. communis and this supports its recognition as a variety. The recognition of J. conferta as a variety of J. rigida [J. rigida var. conferta (Parl.) Patschke] is supported by the data. The data also supports the recognition of J. lutchuensis Koidz. [=J. taxifolia var. lutchuensis (Koldz.) Satake] and J. morrisonicola Hayata [=J. squamata var. morrisonicola (Hayata) H.L. Li and H. Keng] at the specific levels. : S 0 3 0 5 -1 9 7 8 ( 01 )0 0 0 8 7 -4 232 R.P. Adams et al. / Biochemical Systematics and Ecology 30 (2002) [231][232][233][234][235][236][237][238][239][240][241]
Journal of Essential Oil Research, 2003
Twenty one accessions of vetiver (Vetiveria zizanioides (L.) Nash, sterile, oil type) and Khus (V... more Twenty one accessions of vetiver (Vetiveria zizanioides (L.) Nash, sterile, oil type) and Khus (V. zizanioides, fertile, non-oil type) were analyzed by the use of random amplified polymorphic DNAs (RAPDs). Nineteen of the accessions clustered strongly around the cultigen, Sunshine. Three accessions, Khus, Northern India, Kassel, Germany, and Guang Dong, China clustered loosely and were not closely related to the sterile oil producing vetivers. Thirteen of the vetiver accessions were grown in test plots in Florida, USA, Nepal and Portugal. The largest growth was recorded in Nepal, followed closely by Florida and by the cooler, Mediterranean site in Portugal. No single genotype (DNA cultivar) grew best in every plot. Oil yields (% oil/dry wt.) were highest in Nepal and Portugal. Oil yields ranged from 0.29% to 9.61%. Essential oil production (g/plant) was highest in Nepal and Florida and ranged from 0.02 to 4.17 (g/plant). Analyses of variation among the major compounds is discussed.
Biochemical Systematics and Ecology, 2003
Juniperus communis L. var. communis, J. c. var. depressa Pursh, J. c. var. hemispherica J. & C. P... more Juniperus communis L. var. communis, J. c. var. depressa Pursh, J. c. var. hemispherica J. & C. Presl, J. communis var. megistocarpa Fern. & St. John, J. c. var. nipponica (Maxim.) Wils., J. c. var. oblonga hort. ex Loudon and J. c. var. saxatilis Pall. were sampled and DNA fingerprinting (RAPDs, Random Amplified Polymorphic DNAs) was performed.
Plant Molecular Biology Reporter, 1996
A survey of the inhibition of the amplification of spinach DNA by various plant polysaccharides r... more A survey of the inhibition of the amplification of spinach DNA by various plant polysaccharides revealed that neutral polysaccharides (arabinogalactan, dextran, gum guar, gum locust bean, inulin, mannan, and starch) were not inhibitory. In contrast, the acidic polysaccharides (carrageenan, dextran sulfate, gum ghatti, gum karaya, pectin, and xylan)were inhibitory. In the process of preparing random amplified polymorphic DNAs (RAPDs), the loss of large DNA bands appears to be an indicator that the fingerprint pattern has been affected by polysaccharides. The addition of various concentrations of Tween 20, DMSO, or PEG 400 to the PCR reaction mixture resulted in partial restoration of amplification of RAPDs for the acidic polysaccharides. The most effective way to eliminate the effects of polysaccharide inhibition was by diluting the DNA extracts, and thereby diluting the polysaccharide inhibitors.
Biochemical Systematics and Ecology, 2005
Analyses of individuals classically treated as Juniperus oxycedrus L. var. oxycedrus from Morocco... more Analyses of individuals classically treated as Juniperus oxycedrus L. var. oxycedrus from Morocco, Portugal, Spain, France, Italy, Greece and Turkey, using DNA sequencing of nrDNA (ITS 1, 5.8S, ITS 2) plus RAPDs, leaf terpenoids and morphology revealed that two cryptic, genetically distinct but morphologically almost identical species are present. These species, J. oxycedrus L. var. oxycedrus and Juniperus deltoides R.P. Adams, are about as different from each other as Juniperus navicularis and Juniperus macrocarpa are from J. oxycedrus var. oxycedrus. Examination of herbarium specimens revealed that the two species are largely allopatric with J. deltoides occurring from Italy eastward through Turkey into the Caucasus Mts. and Iran. J. oxycedrus var. oxycedrus appears to be largely concentrated west of Italy (France, Spain, Portugal, Morocco). Cryptic speciation is discussed.
Biochemical Systematics and Ecology, 2003
Twelve populations of Juniperus communis L. were sampled from throughout the arctic, worldwide an... more Twelve populations of Juniperus communis L. were sampled from throughout the arctic, worldwide and DNA fingerprinting (RAPDs, Random Amplified Polymorphic DNAs) was performed. Based on 152 RAPD bands, all of the populations (J. communis var. depressa Pursh and J. communis var. megistocarpa Fern. and St. John) from the western hemisphere formed one group and all of the populations of the eastern hemisphere (including Greenland and Iceland), formed another group that included J. communis var. communis and J. communis var. saxatilis Pall., except for the Kamchatka population that was quite dissimilar to any population examined. Most likely, the current sites of all of the populations were covered with ice or otherwise inhospitable, up to or during the late Pleistocene (ca. 12,000 BP). Therefore, these populations are recent in origin. The path of re-colonization appears to have been northward in North America. Greenland appears to have been colonized from Iceland plants, which in turn came from northern Europe. The Kamchatka population seems likely to have come from Japan.
Biochemical Systematics and Ecology, 2003
Random amplified polymorphic DNAs (RAPDs) data were analyzed from all the taxa of Cupressus from ... more Random amplified polymorphic DNAs (RAPDs) data were analyzed from all the taxa of Cupressus from the western hemisphere. Populations of Cupressus from Arizona and Texas, USA, were found to cluster in the two groups delimited by Wolf (1948): C. arizonica and C. glabra. These data suggest that these taxa might be better recognized at the specific level as per Wolf (1948), rather than at the varietal level or not at all .
Biochemical Systematics and Ecology, 2003
Twelve individual genotypes selected from Juniperus populations, varieties and species were analy... more Twelve individual genotypes selected from Juniperus populations, varieties and species were analyzed using ITS sequences, RAPDs, ISSRs, and leaf volatile terpenoids. These four data sets, all analyzed in the same manner, illustrated that these data sets can be used at different organizational levels: specific, inter-specific and intraspecific. Similarity matrices for the ITS, RAPD, and ISSR data sets were highly correlated (r ranged from 0.83 to 0.95). In contrast, the terpenoid matrix had a low correlation with the other data matrices (r ranged from Ϫ0.04 to 0.38). Because these 12 genotypes were taken from several species, the terpenoids, having been shown to most useful at the infra-specific level, were not concordant at this taxonomic level. The high correlation between DNA markers and ITS sequence data implies that these data are measuring concordant patterns among these genotypes.
Biochemical Systematics and Ecology, 2003
Random Amplified Polymorphic DNAs (RAPDs) data were analyzed from eighteen taxa of
Journal of Essential Oil Research, 2003
Samples of Juniperus thurifera L. were collected from the Atlas Mts., Morocco, northern and south... more Samples of Juniperus thurifera L. were collected from the Atlas Mts., Morocco, northern and southern Spain, the Pyrenees, France, Fench Alps and Corse Isaland, France. The leaf oils were analyzed and found to be polymorphic for several major compounds (sabinene, limonene, linalool, piperitone, linalyl acetate and sesquiterpenes). In general, the Moroccan trees were higher in sabinene, γ-terpinene, cis-sabinene hydrate and terpinen-4-ol, but lower in limonene, δ-2-carene, and piperitone than trees from Europe. Analysis based on Random Amplified Polymorphic DNAs (RAPDs) for the aforementioned population plus J. foetidissima (as an outgroup), revealed that the Moroccan J. thurifera populations were most similar to plants from southern Spain, then to populations from France. Although the trees generally clustered by populations, there appear to be some differentiation in the RAPDs between the European J. thurifera populations and the Moroccan populations. Combining previous studies on seeds per cone, proanthrocyanidins, and the current report on the leaf essential oils and RAPDs, there is some support for the continued recognition of J. thurifera var. africana Maire [syn.: J. Africana (Maire); J. thurifera ssp. africana (Maire) Gauquelin, Hassani et Lebreton] in Algeria and Morocco.
Biochemical Systematics and Ecology, 2002
DNA was examined by RAPD banding for Junipers chinensis, J.c. var. sargentii, J.c. var. tsukusien... more DNA was examined by RAPD banding for Junipers chinensis, J.c. var. sargentii, J.c. var. tsukusiensis, J. communis, J.c. var. nipponica, J.c. var. saxatilis, J. conferta, J. formosana, J. procumbens, J. rigida, J. taxifolia and J.t. var. lutchuensis. The DNA data readily separated junipers of section Sabina from section Juniperus. J.c. var. tsukusiensis from Taiwan was found to be sufficiently different from J.c. var. tsukusiensis (Yakushima) to warrant the recognition of a new variety: J. chinensis var. taiwanensis R.P. Adams and C-F. Hsieh nov. var. Juniperus formosana from mainland China was found to be different from J. formosana from Taiwan and a new variety is recognized: J. formosana var. mairei (Lemee and Lev.) R.P. Adams and C-F. Hsieh comb. nov. Juniperus communis var. nipponica was found to be very distinct from J. communis and this supports its recognition as a variety. The recognition of J. conferta as a variety of J. rigida [J. rigida var. conferta (Parl.) Patschke] is supported by the data. The data also supports the recognition of J. lutchuensis Koidz. [=J. taxifolia var. lutchuensis (Koldz.) Satake] and J. morrisonicola Hayata [=J. squamata var. morrisonicola (Hayata) H.L. Li and H. Keng] at the specific levels. : S 0 3 0 5 -1 9 7 8 ( 01 )0 0 0 8 7 -4 232 R.P. Adams et al. / Biochemical Systematics and Ecology 30 (2002) [231][232][233][234][235][236][237][238][239][240][241]