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Research paper thumbnail of Improved hatchery and grow-out technology for marine finfish aquaculture in the Asia-Pacific region

This project focussed on improving marine finfish aquaculture production in the Asia-Pacific regi... more This project focussed on improving marine finfish aquaculture production in the Asia-Pacific region by focussing on key constraints: improving hatchery technology to improve the availability of seedstock; evaluating the nutritional needs of groupers to support the development of compounded pellet diets; and improving communication and coordination of marine finfish aquaculture research and development activities in the Asia-Pacific region.

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Research paper thumbnail of Arthur or Martha? Social structure affects reproductive development and sex change in giant grouper (Epinephelus lanceolatus)

• Aromatase inhibition is effective, but impractical for large-scale sex change of giant grouper.... more • Aromatase inhibition is effective, but impractical for large-scale sex change of giant grouper. • Giant grouper show primary male development in both AI and control groups, indicating they are diandric protogynous hermaphrodites. • Behavioural interactions established a social hierarchy which stimulated sex change. • Treatment averages would not have revealed the full effects of social dynamics on sex change. Conclusion Results

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Research paper thumbnail of Longitudinal field survey during deployment of an emergency autogenous vaccine against Betanodavirus in farmed giant grouper (Epinephelus lanceolatus): Multiple factors contribute to outbreaks and survival

Aquaculture, 2021

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Research paper thumbnail of SS-strain rotifer culture for finfish larvae with small mouth gape

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Research paper thumbnail of Nursery management of grouper: a best-practice manual

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Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural …, 2006

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Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural Engineering - AQUACULT ENG, 2006

A novel technique was developed for the flocculation of marine microalgae commonly used in aquacu... more A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5mgL−1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies ≥80%. The process was rapid, simple and inexpensive, and relatively cost neutral with increasing volume (cf. concentration by centrifugation). Harvested material was readily disaggregated to single cell suspensions by dilution in seawater and mild agitation. Microscopic examination of the cells showed them to be indistinguishable from corre...

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Research paper thumbnail of A transcriptomic investigation of appetite-regulation and digestive processes in giant grouper Epinephelus lanceolatus during early larval development

Journal of fish biology, 2018

The giant grouper Epinephelus lanceolatus is an ecologically vulnerable species with high market ... more The giant grouper Epinephelus lanceolatus is an ecologically vulnerable species with high market demand. However, efforts to improve larval husbandry are hindered by a lack of knowledge surrounding larval developmental physiology. To address this shortfall, a transcriptomic approach was applied to larvae between 1 and 14 days post hatch (dph) to characterise the molecular ontogenesis of genes that influence appetite and digestion. Appetite regulating factors were detected from 1 dph, including neuropeptide Y, nesfatin-1, cocaine and amphetamine regulated transcript, cholecystokinin and pituitary adenylate cyclase activating peptide and the expression level of several genes changed sharply with the onset of exogenous feeding. The level of expression for proteases, chitinases, lipases and amylases typically followed one of two expression patterns, a general increase as development progressed, or an inverted U-shape with maximal expression at c. 6 dph. Similarly, the tendency among bot...

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Research paper thumbnail of Isolation of Australian microalgae and preparation of microalgal concentrates for use as aquaculture feeds

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Research paper thumbnail of A study into parental assignment of the communal spawning protogynous hermaphrodite, giant grouper (Epinephelus lanceolatus)

Aquaculture, 2016

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Research paper thumbnail of Molecular and morphological evidence for the widespread distribution of Laticola paralatesi infecting wild and farmed Lates calcarifer in Australia

Diseases of aquatic organisms, Jan 8, 2015

Infections with monogeneans of the Diplectanidae can limit productivity of and cause considerable... more Infections with monogeneans of the Diplectanidae can limit productivity of and cause considerable health issues for fish in aquaculture. To date, 9 species of diplectanids have been reported from the Asian sea bass or barramundi Lates calcarifer (Perciformes: Latidae) in the Asia-Pacific region. This study characterised the diplectanid parasite fauna found infecting wild and farmed barramundi from 5 localities in tropical Australia, including north Queensland and Western Australia. A combination of morphometric and comparative genetic analyses of partial 28S ribosomal RNA (28S rRNA) from specimens recovered were used to confirm their identity and to explore relationships with other diplectanids. These data revealed that a single, dominant species of diplectanid, Laticola paralatesi, infects wild and farmed Lates calcarifer in tropical Australia. Laticola lingaoensis Yang, Kritsky, Sun, Jiangying, Shi & Agrawal, 2006 is synonymised with L. seabassi (Wu, Li, Zhu & Xie, 2005) Domingues...

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Research paper thumbnail of 8. Research in Progress at the Live Prey Research Unit, Qdpi Northern Fisheries Centre, Cairns

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Research paper thumbnail of Periplasmic proteins of Rhizobium : Variation with growth conditions and use in strain identification

FEMS Microbiology Letters, 1986

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Research paper thumbnail of Flocculated microalgae concentrates as diets for larvae of the tiger prawn Penaeus monodon Fabricius

Aquaculture Nutrition, 2002

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Research paper thumbnail of Development of an optimal microalgal diet for the culture of the calanoid copepod Acartia sinjiensis: Effect of algal species and feed concentration on copepod development

Aquaculture, 2005

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Research paper thumbnail of Isolation of new nanoplanktonic diatom strains and their evaluation as diets for juvenile Pacific oysters (Crassostrea gigas)

Aquaculture, 2002

Ten new diatom strains were isolated from coastal waters of Australia and their potential as feed... more Ten new diatom strains were isolated from coastal waters of Australia and their potential as feed species for aquaculture evaluated based on their cell size, division rate and biochemical composition. All strains were within the size range ingested by most filter feeders, i.e. 3–10 μm. Cellular growth rates of aerated 1.6-l cultures ranged from 0.5–0.7 divisions·day−1 (Papiliocellulus simplex CS-431, Nitzschia

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Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural Engineering, 2006

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Research paper thumbnail of Elevated CO 2 affects the behavior of an ecologically and economically important coral reef fish

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Research paper thumbnail of Improved hatchery and grow-out technology for marine finfish aquaculture in the Asia-Pacific region

This project focussed on improving marine finfish aquaculture production in the Asia-Pacific regi... more This project focussed on improving marine finfish aquaculture production in the Asia-Pacific region by focussing on key constraints: improving hatchery technology to improve the availability of seedstock; evaluating the nutritional needs of groupers to support the development of compounded pellet diets; and improving communication and coordination of marine finfish aquaculture research and development activities in the Asia-Pacific region.

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Research paper thumbnail of Arthur or Martha? Social structure affects reproductive development and sex change in giant grouper (Epinephelus lanceolatus)

• Aromatase inhibition is effective, but impractical for large-scale sex change of giant grouper.... more • Aromatase inhibition is effective, but impractical for large-scale sex change of giant grouper. • Giant grouper show primary male development in both AI and control groups, indicating they are diandric protogynous hermaphrodites. • Behavioural interactions established a social hierarchy which stimulated sex change. • Treatment averages would not have revealed the full effects of social dynamics on sex change. Conclusion Results

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Longitudinal field survey during deployment of an emergency autogenous vaccine against Betanodavirus in farmed giant grouper (Epinephelus lanceolatus): Multiple factors contribute to outbreaks and survival

Aquaculture, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of SS-strain rotifer culture for finfish larvae with small mouth gape

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Nursery management of grouper: a best-practice manual

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural …, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural Engineering - AQUACULT ENG, 2006

A novel technique was developed for the flocculation of marine microalgae commonly used in aquacu... more A novel technique was developed for the flocculation of marine microalgae commonly used in aquaculture. The process entailed an adjustment of pH of culture to between 10 and 10.6 using NaOH, followed by addition of a non-ionic polymer Magnafloc LT-25 to a final concentration of 0.5mgL−1. The ensuing flocculate was harvested, and neutralised giving a final concentration factor of between 200- and 800-fold. This process was successfully applied to harvest cells of Chaetoceros calcitrans, C. muelleri, Thalassiosira pseudonana, Attheya septentrionalis, Nitzschia closterium, Skeletonema sp., Tetraselmis suecica and Rhodomonas salina, with efficiencies ≥80%. The process was rapid, simple and inexpensive, and relatively cost neutral with increasing volume (cf. concentration by centrifugation). Harvested material was readily disaggregated to single cell suspensions by dilution in seawater and mild agitation. Microscopic examination of the cells showed them to be indistinguishable from corre...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A transcriptomic investigation of appetite-regulation and digestive processes in giant grouper Epinephelus lanceolatus during early larval development

Journal of fish biology, 2018

The giant grouper Epinephelus lanceolatus is an ecologically vulnerable species with high market ... more The giant grouper Epinephelus lanceolatus is an ecologically vulnerable species with high market demand. However, efforts to improve larval husbandry are hindered by a lack of knowledge surrounding larval developmental physiology. To address this shortfall, a transcriptomic approach was applied to larvae between 1 and 14 days post hatch (dph) to characterise the molecular ontogenesis of genes that influence appetite and digestion. Appetite regulating factors were detected from 1 dph, including neuropeptide Y, nesfatin-1, cocaine and amphetamine regulated transcript, cholecystokinin and pituitary adenylate cyclase activating peptide and the expression level of several genes changed sharply with the onset of exogenous feeding. The level of expression for proteases, chitinases, lipases and amylases typically followed one of two expression patterns, a general increase as development progressed, or an inverted U-shape with maximal expression at c. 6 dph. Similarly, the tendency among bot...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Isolation of Australian microalgae and preparation of microalgal concentrates for use as aquaculture feeds

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A study into parental assignment of the communal spawning protogynous hermaphrodite, giant grouper (Epinephelus lanceolatus)

Aquaculture, 2016

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular and morphological evidence for the widespread distribution of Laticola paralatesi infecting wild and farmed Lates calcarifer in Australia

Diseases of aquatic organisms, Jan 8, 2015

Infections with monogeneans of the Diplectanidae can limit productivity of and cause considerable... more Infections with monogeneans of the Diplectanidae can limit productivity of and cause considerable health issues for fish in aquaculture. To date, 9 species of diplectanids have been reported from the Asian sea bass or barramundi Lates calcarifer (Perciformes: Latidae) in the Asia-Pacific region. This study characterised the diplectanid parasite fauna found infecting wild and farmed barramundi from 5 localities in tropical Australia, including north Queensland and Western Australia. A combination of morphometric and comparative genetic analyses of partial 28S ribosomal RNA (28S rRNA) from specimens recovered were used to confirm their identity and to explore relationships with other diplectanids. These data revealed that a single, dominant species of diplectanid, Laticola paralatesi, infects wild and farmed Lates calcarifer in tropical Australia. Laticola lingaoensis Yang, Kritsky, Sun, Jiangying, Shi & Agrawal, 2006 is synonymised with L. seabassi (Wu, Li, Zhu & Xie, 2005) Domingues...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of 8. Research in Progress at the Live Prey Research Unit, Qdpi Northern Fisheries Centre, Cairns

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Periplasmic proteins of Rhizobium : Variation with growth conditions and use in strain identification

FEMS Microbiology Letters, 1986

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Flocculated microalgae concentrates as diets for larvae of the tiger prawn Penaeus monodon Fabricius

Aquaculture Nutrition, 2002

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development of an optimal microalgal diet for the culture of the calanoid copepod Acartia sinjiensis: Effect of algal species and feed concentration on copepod development

Aquaculture, 2005

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Isolation of new nanoplanktonic diatom strains and their evaluation as diets for juvenile Pacific oysters (Crassostrea gigas)

Aquaculture, 2002

Ten new diatom strains were isolated from coastal waters of Australia and their potential as feed... more Ten new diatom strains were isolated from coastal waters of Australia and their potential as feed species for aquaculture evaluated based on their cell size, division rate and biochemical composition. All strains were within the size range ingested by most filter feeders, i.e. 3–10 μm. Cellular growth rates of aerated 1.6-l cultures ranged from 0.5–0.7 divisions·day−1 (Papiliocellulus simplex CS-431, Nitzschia

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Production of microalgal concentrates by flocculation and their assessment as aquaculture feeds

Aquacultural Engineering, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Elevated CO 2 affects the behavior of an ecologically and economically important coral reef fish

Bookmarks Related papers MentionsView impact