ROSA MARIA ESPINO MORENO - Academia.edu (original) (raw)

Papers by ROSA MARIA ESPINO MORENO

Monografias De Archivos De Bronconeumologia, Dec 12, 2014

Revista De Ciencias De La Educacion Organo Del Instituto Calasanz De Ciencias De La Educacion, 2008

Praxis Educacional, Feb 10, 2010

El articulo describe y analiza los cambios en la formacion de los profesores de la Educacion Infa... more El articulo describe y analiza los cambios en la formacion de los profesores de la Educacion Infantil y Primaria espanoles para la convergencia en el Espacio Europeo de la Educacion Superior. Las autoras entienden que, Palabras clave: Espacio Europeo de la Educacion Superior. Formacion inicial de los maestros espanoles. Proceso de Bolonia. con una financiacion adecuada, el denominado Proceso de Bolonia sera positivo para la formacion de los maestros de educacion infantil y primaria espanoles por varias razones: 1) Porque otorga a Magisterio el mismo nivel universitario (grado) que a otras profesiones: abogado, economista, odontologo, etc.; 2) Porque la convergencia en el Espacio Europeo de la Educacion Superior aumentara las posibilidades de empleo de nuestros titulados llevandolas mas alla de nuestro propio pais; 3) Porque facilita la movilidad de los estudiantes entre las universidades europeas; 4) Porque al ampliar en un ano la formacion de los maestros (antes eran tres anos y ahora pasan a ser cuatro) permite introducir importantes mejoras en los planes de estudios; 5) Porque es positivo el cambio de un paradigma centrado en la ensenanza del profesor a un paradigma centrado en el aprendizaje del estudiante; 6) Porque aun cuando el enfoque de competencias sea en algunos aspectos cuestionable, esta trayendo consigo una fuerte toma de conciencia de la necesidad de dar pasos eficaces en la necesaria integracion de la teoria y la practica.

La Practica Cotidiana En Los Colegios Casos Para La Formacion De Docentes 2011 Isbn 978 989 26 0127 4 Pags 19 105, 2011

Neural development, Jan 22, 2014

In the spinal cord, stereotypic patterns of transcription factor expression uniquely identify neu... more In the spinal cord, stereotypic patterns of transcription factor expression uniquely identify neuronal subtypes. These transcription factors function combinatorially to regulate gene expression. Consequently, a single transcription factor may regulate divergent development programs by participation in different combinatorial codes. One such factor, the LIM-homeodomain transcription factor Islet1, is expressed in the vertebrate spinal cord. In mouse, chick and zebrafish, motor and sensory neurons require Islet1 for specification of biochemical and morphological signatures. Little is known, however, about the role that Islet1 might play for development of electrical membrane properties in vertebrates. Here we test for a role of Islet1 in differentiation of excitable membrane properties of zebrafish spinal neurons. We focus our studies on the role of Islet1 in two populations of early born zebrafish spinal neurons: ventral caudal primary motor neurons (CaPs) and dorsal sensory Rohon-Be...

eLife, 2015

Myopathies decrease muscle functionality. Mutations in ryanodine receptor 1 (RyR1) are often asso... more Myopathies decrease muscle functionality. Mutations in ryanodine receptor 1 (RyR1) are often associated with myopathies with microscopic core-like structures in the muscle fiber. In this study, we identify a mouse RyR1 model in which heterozygous animals display clinical and pathological hallmarks of myopathy with core-like structures. The RyR1 mutation decreases sensitivity to activated calcium release and myoplasmic calcium levels, subsequently affecting mitochondrial calcium and ATP production. Mutant muscle shows a persistent potassium leak and disrupted expression of regulators of potassium homeostasis. Inhibition of KATP channels or increasing interstitial potassium by diet or FDA-approved drugs can reverse the muscle weakness, fatigue-like physiology and pathology. We identify regulators of potassium homeostasis as biomarkers of disease that may reveal therapeutic targets in human patients with myopathy of central core disease (CCD). Altogether, our results suggest that ameli...

Acta Scientiarum. Technology, 2012

This study accomplished a comparison between microfiltration and addition of coagulating agents t... more This study accomplished a comparison between microfiltration and addition of coagulating agents to clarify sugar cane juice. Microfiltration tests were carried out using ceramic tubular membranes made with TiO 2 /α-Al 2 O 3 , with pore diameter of 0.2; 0.4 and 0.6 μm. The transmembrane pressures applied were 1.0, 2.0 and 3.0 bar, and temperature was kept constant at 20ºC. Clarification test with addition of coagulating agents were performed with PAC and Ca (OH) 2 at 65ºC. The highest permeate flow was 76 kg h-1 m-2 at 1.0 bar with 0.6 μm-membrane. The clarification process with membranes achieved a reduction of turbidity and color superior to 92 and 16%, respectively. In the clarification by adding coagulating agents we verified a reduction superior to 78 and 46% to turbidity and color, respectively.

Plant Aging, 1990

Formation of pollen in angiosperms takes place in the anthers, the male sexual organs. During nor... more Formation of pollen in angiosperms takes place in the anthers, the male sexual organs. During normal male gametophytic development in vivo, each diploid microspore mother cell generates, through meiosis, four haploid microspores; after one or two mitosis and maturation, they will eventually give rise to mature pollen grains. However, in in vitro cultures of isolated anthers or pollen, pollen grains are able to follow a different, sporophytic developmental pathway, leading to the formation of embryos and haploid plants directly and asexually. Recent advances in the technique of isolated pollen culture in the model plant tobacco, allow the strict control of pollen development in both the sporophytic and the gametophytic direction.

Sexual Reproduction in Higher Plants, 1988

Anther culture is one of the few biotechnologies which has found its way into breeding practice. ... more Anther culture is one of the few biotechnologies which has found its way into breeding practice. Still however, many species are recalcitrant and yields are low, indicating that the mechanisms by which immature male gametophytes change their development pathway to form a sporophyte directly and asexually, are not well understood.

Archivos de Bronconeumología ((English Edition)), 2005

Current Plant Science and Biotechnology in Agriculture, 1990

In most transformation systems today, in vitro regeneration of plants is an essential step and ve... more In most transformation systems today, in vitro regeneration of plants is an essential step and very often the main limiting factor for yield and success. Circumvention of in vitro regeneration would therefore be highly advantageous. The simplest way to do this would be to transfer DNA into the gametes and, after gamete fusion, to exploit seed embryogenesis for plant formation. If feasable, such an approach would be technically simpler and faster than methods based on in vitro regeneration. It should also be universal in its application. In addition, one would avoid the problem of somaclonal variation resulting from in vitro regeneration. Somaclonal variation in transgenic commercial lines of crop plants can spoil the advantage obtained by transferring a transgene. The problem of chimaera that is intrinsic to in vitro regeneration, would also be avoided. Gametes in the strict sense are not easily accessible in plants although progress has been achieved in isolating both sperm and egg cells (1). Both, male as well as female gametes, can be used for gene transfer. The main advantage of the egg cell might be the efficient formation of a whole plant either in situ or after isolation and in vitro culture. The latter remains to be shown, however. The male gamete in higher plants is not a free, mobile cell as in lower plants or in animals, but is part of a larger structure, the pollen. And only the pollen as such is able to perform fertilization in situ, while the isolated sperm cell would have to be fused in vitro with the egg cell. Attempts to use male gametes for gene transfer have therefore concentrated on the transfer through pollen.

Transgenic Research, 1992

... Biochim. Biophys. Acta 708, 124-33. Alwen, A., Eller, N., Kastler, M., Benito Moreno, R.-M. a... more ... Biochim. Biophys. Acta 708, 124-33. Alwen, A., Eller, N., Kastler, M., Benito Moreno, R.-M. and Herberle-Bors, E. (1990) Potential of in vitro pollen matur-ation for gene transfer. Physiol. ... Biochem. J. 58, 462-9. Levvy, GA and Marsh, CA (1960) ~-glucuronidase. ...

Theoretical and Applied Genetics, 1991

Immature pollen of two varieties of Triticum aestivum, at the stage right after the first pollen ... more Immature pollen of two varieties of Triticum aestivum, at the stage right after the first pollen mitosis, was isolated from individual anthers and cultured in microcultures of microliter droplets. In a specifically designed medium, some of the pollen grains developed to maturity. These were applied to excised stigmas on agar, where they produced pollen tubes. Application to flowers in vivo led to seed set. Pollen was matured in vitro from a variety that produced a different protein banding pattern on SDS-PAGE as compared to the variety that was pollinated. The protein banding in the produced seeds showed the hybrid pattern, demonstrating that the seeds were not produced by self-pollination in this in-breeding species but by pollination with the in-vitro-matured pollen.

The Journal of Neuroscience, 2011

Inherited loss of P/Q-type calcium channel function causes human absence epilepsy, episodic dyski... more Inherited loss of P/Q-type calcium channel function causes human absence epilepsy, episodic dyskinesia, and ataxia, but the molecular “birthdate” of the neurological syndrome and its dependence on prenatal pathophysiology is unknown. Since these channels mediate transmitter release at synapses throughout the brain and are expressed early in embryonic development, delineating the critical circuitry and onset underlying each of the emergent phenotypes requires targeted control of gene expression. To visualize P/Q-type Ca2+channels and dissect their role in neuronal networks at distinct developmental stages, we created a novel conditionalCacna1aknock-in mouse by inserting the floxed green fluorescent protein derivative Citrine into the first exon ofCacna1aand then crossed it with a postnatally expressing PCP2-Cre line for delayed Purkinje cell (PC) gene deletion within the cerebellum and sparsely in forebrain (purky). PCs inpurkymice lacked P/Q-type calcium channel protein and currents...

Planta, 1988

Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct s... more Immature tobacco (Nicotiana tabacum L.) pollen has been isolated from anthers in three distinct stages of development, including the microspore stage. In in-vitro cultures, fully functional, mature pollen was obtained. In a germination medium, this pollen produced pollen tubes. After application to stigmas in situ, the in-vitro-matured pollen fertilized ovules, and seeds were produced. Genetic tests with seedlings obtained from pollinations with in-vitro-matured pollen from a transgenic plant revealed normal Mendelian segregation of two marker genes, the neomycin-phosphotransferase II gene and the nopaline-synthase gene. These results are of interest with respect to the control of self-incompatibility, cytoplasmic male sterility and pollen-allergen formation, and it offers an alternative route for gene transfer in those plants which cannot be regenerated in vitro.

Plant Physiology, 1992

The effect of anther-derived substances on pollen function was studied using pollen produced by i... more The effect of anther-derived substances on pollen function was studied using pollen produced by in vitro culture of immature pollen of tobacco (Nicotiana tabacum L.) and petunia (Petunia hybrida). Addition of conditioned medium consisting of diffusates from in situ matured pollen strongly increased pollen germination frequency and pollen tube growth, as well as seed set after in situ

Journal of Biological Chemistry, 2005

Voltage-gated Ca 2؉ channels of the N-, P/Q-, and Rtype and G protein inwardly rectifying K ؉ cha... more Voltage-gated Ca 2؉ channels of the N-, P/Q-, and Rtype and G protein inwardly rectifying K ؉ channels (GIRK) are modulated via direct binding of G proteins. The modulation is mediated by G protein ␤␥ subunits. By using electrophysiological recordings and fluorescence resonance energy transfer, we characterized the modulatory domains of the G protein ␤ subunit on the recombinant P/Q-type channel and GIRK channel expressed in HEK293 cells and on native non-L-type Ca 2؉ currents of cultured hippocampal neurons. We found that G␤ 2 subunit-derived deletion constructs and synthesized peptides can either induce or inhibit G protein modulation of the examined ion channels. In particular, the 25-amino acid peptide derived from the G␤ 2 N terminus inhibits G protein modulation, whereas a 35amino acid peptide derived from the G␤ 2 C terminus induced modulation of voltage-gated Ca 2؉ channels and GIRK channels. Fluorescence resonance energy transfer (FRET) analysis of the live action of these peptides revealed that the 25-amino acid peptide diminished the FRET signal between G protein ␤ 2 ␥ 3 subunits, indicating a reorientation between G protein ␤ 2 ␥ 3 subunits in the presence of the peptide. In contrast, the 35-amino acid peptide increased the FRET signal between GIRK1,2 channel subunits, similarly to the G␤␥-mediated FRET increase observed for this GIRK subunit combination. Circular dichroism spectra of the synthesized peptides suggest that the 25-amino acid peptide is structured. These results indicate that individual G protein ␤ subunit domains can act as independent, separate modulatory domains to either induce or inhibit G protein modulation for several effector proteins.

Journal of Biological Chemistry, 2003

Presynaptic Ca 2؉ channels are inhibited by metabotropic receptors. A possible mechanism for this... more Presynaptic Ca 2؉ channels are inhibited by metabotropic receptors. A possible mechanism for this inhibition is that G protein ␤␥ subunits modulate the binding of the Ca 2؉ channel ␤ subunit on the Ca 2؉ channel complex and induce a conformational state from which channel opening is more reluctant. To test this hypothesis, we analyzed the binding of Ca 2؉ channel ␤ and G protein ␤ subunits on the two separate binding sites, i.e. the loopI-II and the C terminus, and on the full-length P/Q-type ␣ 1 2.1 subunit by using a modified mammalian two-hybrid system and fluorescence resonance energy transfer (FRET) measurements. Analysis of the interactions on the isolated bindings sites revealed that the Ca 2؉ channel ␤ 1b subunit induces a strong fluorescent signal when interacting with the loopI-II but not with the C terminus. In contrast, the G protein ␤ subunit induces FRET signals on both the C terminus and loopI-II. Analysis of the interactions on the full-length channel indicates that Ca 2؉ channel ␤ 1b and G protein ␤ subunits bind to the ␣ 1 subunit at the same time. Coexpression of the G protein increases the FRET signal between ␣ 1 /␤ 1b FRET pairs but not for ␣ 1 /␤ 1b FRET pairs where the C terminus was deleted from the ␣ 1 subunit. The results suggest that the G protein alters the orientation and/or association between the Ca 2؉ channel ␤ and ␣ 1 2.1 subunits, which involves the C terminus of the ␣ 1 subunit and may corresponds to a new conformational state of the channel.

Journal of Bioenergetics and Biomembranes, 2003

Ca 2+ channels mediate the voltage-dependent Ca 2+ influx in subcellular compartments, triggering... more Ca 2+ channels mediate the voltage-dependent Ca 2+ influx in subcellular compartments, triggering such diverse processes as neurotransmitter release, dendritic action potentials, excitation-contraction, and excitation-transcription coupling. The targeting of biophysically defined Ca 2+ channel complexes to the correct subcellular structures is, thus, critical to proper cell and physiological functioning. Despite their importance, surprisingly little is known about the targeting mechanisms by which Ca 2+ channel complexes are trausported to their site of function. Here we summarize what we know about the targeting of Ca 2+ channel complexes through the cell to the plasma membrane and subcellular structures. KEY WORDS: Ca 2+ channels; channel targeting; α 1 and β subunits; heterologous expression systems; striated muscle; hippocampal neurons.