R. Polignano - Academia.edu (original) (raw)
Papers by R. Polignano
Intracellular free calcium, [Ca 2 + ],, was studied in platelets of essential hypertensive subjec... more Intracellular free calcium, [Ca 2 + ],, was studied in platelets of essential hypertensive subjects and normotensive controls under basal conditions and after stimulation with epinephrine, norepinephrine, angiotensin II, ouabain, and thrombin, using the fluorescent calcium indicator quin 2. Basal [Ca 2+ ], was significantly higher in hypertensive subjects (n = 32) than in normotensive controls (n = 30; 167.4 ± 5.0 vs 143.2 ± 3.1 nmol/L; p<0.001). Epinephrine, norepinephrine, angiotensin II, and ouabain had no effect on platelet calcium, whereas thrombin induced a dose-dependent increase in [Ca 2+ ]| in both the presence and absence of extracellular calcium. This [Ca 2+ ]| increase in the presence of extracellular calcium, which depends mainly on calcium influx, was significantly higher (p<0.05) in platelets of hypertensive subjects at all thrombin concentrations (ranging from 0.025-0.1 U/ml), while the [Ca 2+ ]| increase in the absence of extracellular calcium, which depends only on release from intracellular stores, was similar in hypertensive subjects and controls. These results suggest that, in essential hypertension, there is not only increased platelet resting [Ca 2+ ]| but also an increase in agonist-mediated calcium influx, which appears to indicate a cell membrane abnormality in the platelets of subjects with essential hypertension. (Hypertension 9: 230-235, 1987) KEY WORDS • essential hypertension • intracellular calcium • thrombin • platelets • quin 2 • calcium influx • epinephrine • norepinephrine • ouabain • angiotensin II T HE importance of calcium ions in the pathogenesis of arterial hypertension has long been recognized (for a review, see Reference 1). Increased free cytosolic calcium concentration, [Ca 2+ ]j, is related to an increase in active tension of smooth muscle cells and is therefore responsible for an increase in arteriolar resistance. 2 -3 [Ca 2+ ]i is also im-
Journal of Wound Care, 2004
This study set out to compare healing rates, handling properties and patient comfort of a four-la... more This study set out to compare healing rates, handling properties and patient comfort of a four-layer bandage system (Profore) and Unna's Boot in the treatment of venous leg ulcers. This was a prospective randomised parallel-group trial. Patients with venous leg ulcers from four centres in Italy were randomised to receive treatment with either Unna's Boot or Profore. Time to healing was recorded for a maximum of 24 weeks. Pain was assessed using a visual analogue scale. Staff assessed ease of application, while patients rated comfort. Data were available for 68 patients with 68 wounds. At 24 weeks complete healing was seen in 74% of the Profore group compared with 66% of the Unna's Boot group. The median time spent in the study was 50 days (range: 7-175 days), corresponding to 51 days (range: 7-175 days) for Profore patients and 49 days (range: 7-168 days) for Unna's Boot patients. There was no difference (p = 0.13) between the groups in time to closure. Overall, more Profore than Unna's Boot applications were rated as excellent. There was a significant difference, in favour of Profore, in ease of application at the final application (p = 0.013). At the initial application, there was a significant difference in bandage appearance (p = 0.04), again favouring Profore, but this was not sustained at the final application (p = 0.18). Profore is as effective as Unna's Boot in the treatment of venous leg ulcers.
Journal of Wound Care, 2004
Journal of Wound Care, 2009
To compare the effect on the microcirculation in venous leg ulcers (VLUs) of two treatment regime... more To compare the effect on the microcirculation in venous leg ulcers (VLUs) of two treatment regimens that promote a moist wound environment versus paraffin gauze. The hypothesis is that moist wound dressings are more likely to stimulate the microcirculation and therefore angiogenesis. Patients with non-healing VLUs were randomised to receive either a foam dressing (Suprasorb P), a collagen dressing (Suprasorb C) plus the foam dressing, or paraffin gauze (control). All patients wore short-stretch high compression bandages. Parameters used to measure the effects of the treatments on the microcirculation were: TcPO2 measurements, video laser Doppler measurements and the number of capillaries in the wound bed. The progression towards healing was measured by the reduction in ulcer area and formation of granulation tissue. The treatment period was four weeks. Significant increases in TcPO2 values were reported between baseline and week 4 for patients receiving the foam dressing only or the collagen plus foam dressing combination (p<0.008 versus p<0.003 respectively). There was also a significant increase in the number of capillaries for the collagen plus foam treatment only (p<0.002). This pilot study suggests that a moist wound environment stimulates perfusion of blood and oxygen to the wound tissue, thereby promoting angiogenesis. The collagen and foam dressing combination demonstrated superior results to the control and the foam dressing only. Monitoring the microcirculation may help to assess the effect of dressings on VLU healing, although more research is needed. This pilot study was supported by a limited grant from Lohmann & Rauscher GmbH, Germany.
Hypertension, 1987
Intracellular free calcium, [Ca2+]i, was studied in platelets of essential hypertensive subjects ... more Intracellular free calcium, [Ca2+]i, was studied in platelets of essential hypertensive subjects and normotensive controls under basal conditions and after stimulation with epinephrine, norepinephrine, angiotensin II, ouabain, and thrombin, using the fluorescent calcium indicator quin 2. Basal [Ca2+]i was significantly higher in hypertensive subjects (n = 32) than in normotensive controls (n = 30; 167.4 +/- 5.0 vs 143.2 +/- 3.1 nmol/L; p less than 0.001). Epinephrine, norepinephrine, angiotensin II, and ouabain had no effect on platelet calcium, whereas thrombin induced a dose-dependent increase in [Ca2+]i in both the presence and absence of extracellular calcium. This [Ca2+]i increase in the presence of extracellular calcium, which depends mainly on calcium influx, was significantly higher (p less than 0.05) in platelets of hypertensive subjects at all thrombin concentrations (ranging from 0.025-0.1 U/ml), while the [Ca2+]i increase in the absence of extracellular calcium, which depends only on release from intracellular stores, was similar in hypertensive subjects and controls. These results suggest that, in essential hypertension, there is not only increased platelet resting [Ca2+]i but also an increase in agonist-mediated calcium influx, which appears to indicate a cell membrane abnormality in the platelets of subjects with essential hypertension.
Clinica Chimica Acta, 1987
Erythrocyte membrane Na',K+-ATPase activity was measured using a bioluminescence technique in 28 ... more Erythrocyte membrane Na',K+-ATPase activity was measured using a bioluminescence technique in 28 hypertensive patients (24 with essential hypertension, 2 with renovascular hypertension and 2 with hypertension secondary to primary hyperaldosteronism) and in 28 normotensive control subjects matched for age and sex.
Introduzione. Le ulcere venose degli arti inferiori costituiscono un importante problema sanitari... more Introduzione. Le ulcere venose degli arti inferiori costituiscono un importante problema sanitario e sociale colpendo da 1 al 3% della popolazione(1). La guarigione è spesso lenta e circa l'8% risultano ancora aperte a 5 anni(2). Tale andamento cronico, la frequenza ed il costo delle medicazioni e l'impegno del personale medico ed infermieristico ne fanno uno dei problemi sanitari più costosi. Gli studi comparativi in questo campo sono molto difficili a causa dell'impossibilità di studi in doppio cieco da un lato, e dall'altro per la peculiarità della sede, delle caratteristiche dell'ulcera e degli aspetti legati al singolo Paziente. L'unico trattamento che il Cochrain Group per la Based Evidence Medicine ha riscontrato essere efficace è l'elastocompressione(3). Per altri tipi di trattamenti locali esistono sono raccomandazioni o studi osservazionali.
Minerva cardioangiologica, 1999
Thrombosis Research, 1994
1. Thromb Res. 1994 Jul 15;75(2):219-22. Evaluation of prothrombin F1+2 fragment after videolapar... more 1. Thromb Res. 1994 Jul 15;75(2):219-22. Evaluation of prothrombin F1+2 fragment after videolaparoscopic surgery. Vannucchi PL, Ridolfi B, Biliotti G, Naspetti R, Monaldi ML, Burgio G, Polignano R, Sassi R, Prisco D. 2nd ...
Journal of the European Academy of Dermatology and Venereology, 1997
Mi devo congratulare con il gruppo di esperti che hanno sviluppato questo documento di consenso p... more Mi devo congratulare con il gruppo di esperti che hanno sviluppato questo documento di consenso per aver prodotto linee guida chiare ed aggiornate sul trattamento delle ulcere delle gambe mediante terapia compressiva.
Intracellular free calcium, [Ca 2 + ],, was studied in platelets of essential hypertensive subjec... more Intracellular free calcium, [Ca 2 + ],, was studied in platelets of essential hypertensive subjects and normotensive controls under basal conditions and after stimulation with epinephrine, norepinephrine, angiotensin II, ouabain, and thrombin, using the fluorescent calcium indicator quin 2. Basal [Ca 2+ ], was significantly higher in hypertensive subjects (n = 32) than in normotensive controls (n = 30; 167.4 ± 5.0 vs 143.2 ± 3.1 nmol/L; p<0.001). Epinephrine, norepinephrine, angiotensin II, and ouabain had no effect on platelet calcium, whereas thrombin induced a dose-dependent increase in [Ca 2+ ]| in both the presence and absence of extracellular calcium. This [Ca 2+ ]| increase in the presence of extracellular calcium, which depends mainly on calcium influx, was significantly higher (p<0.05) in platelets of hypertensive subjects at all thrombin concentrations (ranging from 0.025-0.1 U/ml), while the [Ca 2+ ]| increase in the absence of extracellular calcium, which depends only on release from intracellular stores, was similar in hypertensive subjects and controls. These results suggest that, in essential hypertension, there is not only increased platelet resting [Ca 2+ ]| but also an increase in agonist-mediated calcium influx, which appears to indicate a cell membrane abnormality in the platelets of subjects with essential hypertension. (Hypertension 9: 230-235, 1987) KEY WORDS • essential hypertension • intracellular calcium • thrombin • platelets • quin 2 • calcium influx • epinephrine • norepinephrine • ouabain • angiotensin II T HE importance of calcium ions in the pathogenesis of arterial hypertension has long been recognized (for a review, see Reference 1). Increased free cytosolic calcium concentration, [Ca 2+ ]j, is related to an increase in active tension of smooth muscle cells and is therefore responsible for an increase in arteriolar resistance. 2 -3 [Ca 2+ ]i is also im-
Journal of Wound Care, 2004
This study set out to compare healing rates, handling properties and patient comfort of a four-la... more This study set out to compare healing rates, handling properties and patient comfort of a four-layer bandage system (Profore) and Unna's Boot in the treatment of venous leg ulcers. This was a prospective randomised parallel-group trial. Patients with venous leg ulcers from four centres in Italy were randomised to receive treatment with either Unna's Boot or Profore. Time to healing was recorded for a maximum of 24 weeks. Pain was assessed using a visual analogue scale. Staff assessed ease of application, while patients rated comfort. Data were available for 68 patients with 68 wounds. At 24 weeks complete healing was seen in 74% of the Profore group compared with 66% of the Unna's Boot group. The median time spent in the study was 50 days (range: 7-175 days), corresponding to 51 days (range: 7-175 days) for Profore patients and 49 days (range: 7-168 days) for Unna's Boot patients. There was no difference (p = 0.13) between the groups in time to closure. Overall, more Profore than Unna's Boot applications were rated as excellent. There was a significant difference, in favour of Profore, in ease of application at the final application (p = 0.013). At the initial application, there was a significant difference in bandage appearance (p = 0.04), again favouring Profore, but this was not sustained at the final application (p = 0.18). Profore is as effective as Unna's Boot in the treatment of venous leg ulcers.
Journal of Wound Care, 2004
Journal of Wound Care, 2009
To compare the effect on the microcirculation in venous leg ulcers (VLUs) of two treatment regime... more To compare the effect on the microcirculation in venous leg ulcers (VLUs) of two treatment regimens that promote a moist wound environment versus paraffin gauze. The hypothesis is that moist wound dressings are more likely to stimulate the microcirculation and therefore angiogenesis. Patients with non-healing VLUs were randomised to receive either a foam dressing (Suprasorb P), a collagen dressing (Suprasorb C) plus the foam dressing, or paraffin gauze (control). All patients wore short-stretch high compression bandages. Parameters used to measure the effects of the treatments on the microcirculation were: TcPO2 measurements, video laser Doppler measurements and the number of capillaries in the wound bed. The progression towards healing was measured by the reduction in ulcer area and formation of granulation tissue. The treatment period was four weeks. Significant increases in TcPO2 values were reported between baseline and week 4 for patients receiving the foam dressing only or the collagen plus foam dressing combination (p<0.008 versus p<0.003 respectively). There was also a significant increase in the number of capillaries for the collagen plus foam treatment only (p<0.002). This pilot study suggests that a moist wound environment stimulates perfusion of blood and oxygen to the wound tissue, thereby promoting angiogenesis. The collagen and foam dressing combination demonstrated superior results to the control and the foam dressing only. Monitoring the microcirculation may help to assess the effect of dressings on VLU healing, although more research is needed. This pilot study was supported by a limited grant from Lohmann & Rauscher GmbH, Germany.
Hypertension, 1987
Intracellular free calcium, [Ca2+]i, was studied in platelets of essential hypertensive subjects ... more Intracellular free calcium, [Ca2+]i, was studied in platelets of essential hypertensive subjects and normotensive controls under basal conditions and after stimulation with epinephrine, norepinephrine, angiotensin II, ouabain, and thrombin, using the fluorescent calcium indicator quin 2. Basal [Ca2+]i was significantly higher in hypertensive subjects (n = 32) than in normotensive controls (n = 30; 167.4 +/- 5.0 vs 143.2 +/- 3.1 nmol/L; p less than 0.001). Epinephrine, norepinephrine, angiotensin II, and ouabain had no effect on platelet calcium, whereas thrombin induced a dose-dependent increase in [Ca2+]i in both the presence and absence of extracellular calcium. This [Ca2+]i increase in the presence of extracellular calcium, which depends mainly on calcium influx, was significantly higher (p less than 0.05) in platelets of hypertensive subjects at all thrombin concentrations (ranging from 0.025-0.1 U/ml), while the [Ca2+]i increase in the absence of extracellular calcium, which depends only on release from intracellular stores, was similar in hypertensive subjects and controls. These results suggest that, in essential hypertension, there is not only increased platelet resting [Ca2+]i but also an increase in agonist-mediated calcium influx, which appears to indicate a cell membrane abnormality in the platelets of subjects with essential hypertension.
Clinica Chimica Acta, 1987
Erythrocyte membrane Na',K+-ATPase activity was measured using a bioluminescence technique in 28 ... more Erythrocyte membrane Na',K+-ATPase activity was measured using a bioluminescence technique in 28 hypertensive patients (24 with essential hypertension, 2 with renovascular hypertension and 2 with hypertension secondary to primary hyperaldosteronism) and in 28 normotensive control subjects matched for age and sex.
Introduzione. Le ulcere venose degli arti inferiori costituiscono un importante problema sanitari... more Introduzione. Le ulcere venose degli arti inferiori costituiscono un importante problema sanitario e sociale colpendo da 1 al 3% della popolazione(1). La guarigione è spesso lenta e circa l'8% risultano ancora aperte a 5 anni(2). Tale andamento cronico, la frequenza ed il costo delle medicazioni e l'impegno del personale medico ed infermieristico ne fanno uno dei problemi sanitari più costosi. Gli studi comparativi in questo campo sono molto difficili a causa dell'impossibilità di studi in doppio cieco da un lato, e dall'altro per la peculiarità della sede, delle caratteristiche dell'ulcera e degli aspetti legati al singolo Paziente. L'unico trattamento che il Cochrain Group per la Based Evidence Medicine ha riscontrato essere efficace è l'elastocompressione(3). Per altri tipi di trattamenti locali esistono sono raccomandazioni o studi osservazionali.
Minerva cardioangiologica, 1999
Thrombosis Research, 1994
1. Thromb Res. 1994 Jul 15;75(2):219-22. Evaluation of prothrombin F1+2 fragment after videolapar... more 1. Thromb Res. 1994 Jul 15;75(2):219-22. Evaluation of prothrombin F1+2 fragment after videolaparoscopic surgery. Vannucchi PL, Ridolfi B, Biliotti G, Naspetti R, Monaldi ML, Burgio G, Polignano R, Sassi R, Prisco D. 2nd ...
Journal of the European Academy of Dermatology and Venereology, 1997
Mi devo congratulare con il gruppo di esperti che hanno sviluppato questo documento di consenso p... more Mi devo congratulare con il gruppo di esperti che hanno sviluppato questo documento di consenso per aver prodotto linee guida chiare ed aggiornate sul trattamento delle ulcere delle gambe mediante terapia compressiva.