R. Rosset - Academia.edu (original) (raw)
Papers by R. Rosset
Digestive and Liver Disease, 2002
Ambulatori Raphael Calcinato (Bs) lntrodunion Faecal occult blood test (FOBT) followed by colonsc... more Ambulatori Raphael Calcinato (Bs) lntrodunion Faecal occult blood test (FOBT) followed by colonscopy in case of poslttve results represents the best available strategy in colorectal cancer screening A low comphance to the protocol in genera, population m often one ofthe factors Iimtting thts approach Moreover, new immunological FOBTs are now under evaluabon, m order to increase sensitiwty ofthe entm procedure Purpose of the study was to evaluate-the wsitive oredictive value (PPVI of the new ~mmunolorx.al FOBT " OC-He&dia I' fdr the early diagnbsis df colorectal neoplasms-(adeooma and carcinoma) in a standard-risk, self-referred population, the compliance to the subsequent diagnostic work-up in case ofFOBT posmwty. the protocol's performance in the subgroup aged 40-49 Methods The study was caned out between Jan ,998 and Dee 2OM) on a population of 717 I asimmomattc sub&s. seed 40-79. self-referred for a medtca, check-w Only X/o oftbem pre&ted some&k for c~lorectal cancer (familial or personal history if polyps aodior cancer) SubIects were asked to collen three samples from three consecutive discharges, and, in cese of one or more positive samples (examined with OC-Hemodm), to perform a whole COhSCOpy
Science
In attempting to define the role of ribosomes in the mechanism of streptomycin dependence, a new ... more In attempting to define the role of ribosomes in the mechanism of streptomycin dependence, a new phenomenon has been discovered. Analysis of this phenomenon-called phenotypic masking-leads to the conclusion that "streptomycin dependent" mutants are actually "drug dependent" because their dependence is equally satisfied by several drugs. These drugs, some of which are totally unrelated chemically, act on the ribosome and induce misreading in vitro and suppression in vivo.
Comptes Rendus de l Académie des Sciences - Series IIC - Chemistry
Physico-Chemical Behaviour of Atmospheric Pollutants, 1990
Atmospheric Environment. Part A. General Topics, 1990
The two-dimensional version of a mesoscale meteorological model is applied to the removal of solu... more The two-dimensional version of a mesoscale meteorological model is applied to the removal of soluble and reactive species by orographicaily forced clouds and precipitation. The model includes both SOland acidity productions within typical maritime clouds. A quasi-spectral microphysical scheme allows for a detailed description of the partitioning of different constituents between air, cloud and rain.
The Biochemical journal, Jan 15, 1978
Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can ... more Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can be isolated that can no longer grow under these conditions. We present here the biochemical characterization of one such mutant and show that the primary genetic lesion occurs in the ubiquinone-8-biosynthetic pathway. As a consequence of this, under aerobic growth conditions the mutant is apparently unable to synthesize formate dehydrogenase, but can synthesize a Benzyl Viologen-dependent nitrate reductase activity. The nature of this activity is discussed.
Bulletin de la Société de chimie biologique, 1964
Journal of chromatography, Jan 14, 1982
Journal of cell science, 1982
Classical electron-microscopic techniques (enzymic digestion, EDTA regressive staining) allied wi... more Classical electron-microscopic techniques (enzymic digestion, EDTA regressive staining) allied with autoradiographic studies after [3H]uridine incorporation or after RNA synthesis initiated by an exogeneous RNA polymerase in the presence of tritiated GTP, enabled us to describe the fine structure and activity of the nucleolus in an established Drosophila cell line. This nucleolus is composed of a large central multilobed core containing proteins, RNA molecules and a DNA-containing component. This core is surrounded by and connected to large clumps of dense fibrillar nucleolus-associated chromatin, which are intermingled with fibrillogranular ramifications extending from the core towards the nuclear envelope. These ramifications are covered by granules of ribosomal ribonucleoprotein. As shown by EDTA regressive staining the nucleolar core contains a ribonucleoprotein network, which unravels and ramifies within a fibrous matrix. RNA synthesis takes place at the level of this network i...
L' Alimentation et la vie, 1967
Journal of chromatography, Jan 26, 1988
An automated liquid chromatographic method for the determination of urinary concentrations of 4-h... more An automated liquid chromatographic method for the determination of urinary concentrations of 4-hydroxy-3-methoxymandelic acid (VMA) is described. Urine samples are purified by solid-phase extraction on an anion-exchange cartridge and automated on-line chromatographic elution is carried out using a Varian AASP (advanced automated sample processor) system. The column effluent is monitored with an electrochemical detector using a glassy carbon working electrode. The method allows the determination of VMA in 0.05 ml of normal urine with a relative standard deviation of less than 3%. The analysis time can be shortened by use of back-flushing technique, and the correlation with a classical (but non-automated) VMA analysis method is excellent.
In order to study the occurrence of the opiate pentapeptide /3-[D-Ala 2'4, Tyr 5] CM-5-NH2 in the... more In order to study the occurrence of the opiate pentapeptide /3-[D-Ala 2'4, Tyr 5] CM-5-NH2 in the 10 -9-10 -5 M concentration range, we have developed a new method consisting of extraction of the peptide from its buffered aqueous medium onto a reversed phase cartridge of 50 #L void volume, followed by on-line injection onto a Cu(ll) modified silica gel column. The acetonitrile-rich mobile phase allows detection of the natural fluorescence of the peptide at the picomole level. dent manner . This peptide acts, like enkephalins, in the 10-9-10 -5 M concentration range. In addition, it has been demonstrated that this peptide is able to cross intact the intestinal mucosa and thus is active from the luminal site of the intestine [8]. The classical RP HPLC method with UV detection at 214 nm previously used for the analysis of the peptide has a detection limit in the 0.1-1 nanomole range. For this reasons the determination of its metabolic fate and transport in vitro or in vivo needed the preconcentration of large volumes before injection in the chromatograph. In the present study, a method has been developed that permits the detection of the peptide in the picomole range. This technique combines a reversed phase preconcentration step to extract the peptide from the physiological medium, followed by ligand exchange chromatography and fluorescence detection at the picomole level; we show here that thiscombination is very powerful and gives reproducible results 9 1 Present address: c/o Pr. Nakanishi,
... 1. Separation of (dC, dT) (34,5,426o-units) on Nucleosil Cxs (7.5 #m) column (250 x 4.6 mm ID... more ... 1. Separation of (dC, dT) (34,5,426o-units) on Nucleosil Cxs (7.5 #m) column (250 x 4.6 mm ID) by isocratic elution with 86% A (A = 0.1 M ammonium acetate) and 14% B (B = methanol-water, 60:40) at room temperature; flow-rate, 1 ml/min. Peaks: I = (dC-dT); 2 = (dT-dC). Fig. ...
Revue des sciences de l'eau, 1999
The EMBO Journal, 1998
We report herein the isolation of ccf, a new gene located in region 82E and essential for Drosoph... more We report herein the isolation of ccf, a new gene located in region 82E and essential for Drosophila development. This gene, expressed throughout development, encodes a novel product of 68 kDa which is found in the nucleus during interphase and labels, in a novel pattern, centrosomes and chromosome arms during mitosis. Mutations in ccf give rise to late larvae with small imaginal discs and to adults showing appendages of reduced size, consistent with CCF involvement in cell proliferation. Neuroblast squash analyses show that CCF is required for proper condensation of mitotic chromosomes and, therefore, for progression through mitosis. Furthermore, we observe that adult ccf mutants as well as animals overexpressing CCF during larval stages exhibit homeotic transformations. We also find that mutations in the Pc-G genes Polycomb, polyhomeotic and Enhancer of zeste are enhanced by ccf mutations. Finally, we show that the CCF protein binds to specific sites on polytene chromosomes, many of which are shared with the Posterior sex combs Pc-G protein. Together, these results suggest a role for the CCF protein in the maintenance of chromosome structure during mitosis and interphase.
Tellus B, 1989
A three-dimensional mesoscale numerical model with detailed parameterized microphysics of clouds ... more A three-dimensional mesoscale numerical model with detailed parameterized microphysics of clouds and precipitation has been applied to the study of gas and aerosol wet removal. Twodimensional sensitivity tests combining meteorological predictions with pollutant scavenging and aqueous chemistry parameterizations have been carried out for continental and maritime clouds over an idealized topography. Model results indicate that nucleation scavenging is the most efficient in-cloud removal mechanism. Furthermore, differences in cloud droplet spectra in continental versus maritime cases, lead to different contributions of nucleation scavenging, in-solution oxidation of SO, by ozone and hydrogen peroxide, to wet sulfate deposition and acidity production. This results emphasizes the need for coupled treatment of dynamical, microphysical and chemical processes.
Tellus B, 2007
A B S T R A C T Few global aerosol models deal with size differentiated inorganic/organic particl... more A B S T R A C T Few global aerosol models deal with size differentiated inorganic/organic particles. Among them, still fewer ones explicitly treat secondary organic aerosol (SOA) formation. In this context, we have coupled the global chemistrytransport model (CTM) TM4 and the aerosol sectional model ORISAM (ORganic and Inorganic Sectional Aerosol Model, Bessagnet et al., 2002). This new aerosol model ORISAM-TM4 can accommodate aerosol size distributions with a variable number of diameter sections (bins) between 0.04 μm and over 10 μm and detailed organic/inorganic chemistry coupled with optional gas schemes. Two model versions are presented: a tracer version and a fully detailed eight-bin version with SOA formation. Focus is made on carbonaceous BC (black carbon) and OC (organic carbon) aerosols. First, significant developments both in ORISAM and in TM4 are discussed in line with the incorporation of updated emission inventories of BC and primary OC (OCp). Then, general comparisons are made between simulated BC and OC concentrations in air and precipitation against worldwide measurements. Also for BC, sensitivity tests using different updated fossil fuel emission inventories are focused over Europe, where emission controls make great strides. The tracer version appears generally satisfactory for BC mostly at background and remote sites, but not for total OC. For this latter, quite significant improvements result from the incorporation of SOA formation in ORISAM-TM4, instead of estimating OC as being simply proportional to OCp, as done in most existing models. Conclusions and prospects are then given.
Digestive and Liver Disease, 2002
Ambulatori Raphael Calcinato (Bs) lntrodunion Faecal occult blood test (FOBT) followed by colonsc... more Ambulatori Raphael Calcinato (Bs) lntrodunion Faecal occult blood test (FOBT) followed by colonscopy in case of poslttve results represents the best available strategy in colorectal cancer screening A low comphance to the protocol in genera, population m often one ofthe factors Iimtting thts approach Moreover, new immunological FOBTs are now under evaluabon, m order to increase sensitiwty ofthe entm procedure Purpose of the study was to evaluate-the wsitive oredictive value (PPVI of the new ~mmunolorx.al FOBT " OC-He&dia I' fdr the early diagnbsis df colorectal neoplasms-(adeooma and carcinoma) in a standard-risk, self-referred population, the compliance to the subsequent diagnostic work-up in case ofFOBT posmwty. the protocol's performance in the subgroup aged 40-49 Methods The study was caned out between Jan ,998 and Dee 2OM) on a population of 717 I asimmomattc sub&s. seed 40-79. self-referred for a medtca, check-w Only X/o oftbem pre&ted some&k for c~lorectal cancer (familial or personal history if polyps aodior cancer) SubIects were asked to collen three samples from three consecutive discharges, and, in cese of one or more positive samples (examined with OC-Hemodm), to perform a whole COhSCOpy
Science
In attempting to define the role of ribosomes in the mechanism of streptomycin dependence, a new ... more In attempting to define the role of ribosomes in the mechanism of streptomycin dependence, a new phenomenon has been discovered. Analysis of this phenomenon-called phenotypic masking-leads to the conclusion that "streptomycin dependent" mutants are actually "drug dependent" because their dependence is equally satisfied by several drugs. These drugs, some of which are totally unrelated chemically, act on the ribosome and induce misreading in vitro and suppression in vivo.
Comptes Rendus de l Académie des Sciences - Series IIC - Chemistry
Physico-Chemical Behaviour of Atmospheric Pollutants, 1990
Atmospheric Environment. Part A. General Topics, 1990
The two-dimensional version of a mesoscale meteorological model is applied to the removal of solu... more The two-dimensional version of a mesoscale meteorological model is applied to the removal of soluble and reactive species by orographicaily forced clouds and precipitation. The model includes both SOland acidity productions within typical maritime clouds. A quasi-spectral microphysical scheme allows for a detailed description of the partitioning of different constituents between air, cloud and rain.
The Biochemical journal, Jan 15, 1978
Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can ... more Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can be isolated that can no longer grow under these conditions. We present here the biochemical characterization of one such mutant and show that the primary genetic lesion occurs in the ubiquinone-8-biosynthetic pathway. As a consequence of this, under aerobic growth conditions the mutant is apparently unable to synthesize formate dehydrogenase, but can synthesize a Benzyl Viologen-dependent nitrate reductase activity. The nature of this activity is discussed.
Bulletin de la Société de chimie biologique, 1964
Journal of chromatography, Jan 14, 1982
Journal of cell science, 1982
Classical electron-microscopic techniques (enzymic digestion, EDTA regressive staining) allied wi... more Classical electron-microscopic techniques (enzymic digestion, EDTA regressive staining) allied with autoradiographic studies after [3H]uridine incorporation or after RNA synthesis initiated by an exogeneous RNA polymerase in the presence of tritiated GTP, enabled us to describe the fine structure and activity of the nucleolus in an established Drosophila cell line. This nucleolus is composed of a large central multilobed core containing proteins, RNA molecules and a DNA-containing component. This core is surrounded by and connected to large clumps of dense fibrillar nucleolus-associated chromatin, which are intermingled with fibrillogranular ramifications extending from the core towards the nuclear envelope. These ramifications are covered by granules of ribosomal ribonucleoprotein. As shown by EDTA regressive staining the nucleolar core contains a ribonucleoprotein network, which unravels and ramifies within a fibrous matrix. RNA synthesis takes place at the level of this network i...
L' Alimentation et la vie, 1967
Journal of chromatography, Jan 26, 1988
An automated liquid chromatographic method for the determination of urinary concentrations of 4-h... more An automated liquid chromatographic method for the determination of urinary concentrations of 4-hydroxy-3-methoxymandelic acid (VMA) is described. Urine samples are purified by solid-phase extraction on an anion-exchange cartridge and automated on-line chromatographic elution is carried out using a Varian AASP (advanced automated sample processor) system. The column effluent is monitored with an electrochemical detector using a glassy carbon working electrode. The method allows the determination of VMA in 0.05 ml of normal urine with a relative standard deviation of less than 3%. The analysis time can be shortened by use of back-flushing technique, and the correlation with a classical (but non-automated) VMA analysis method is excellent.
In order to study the occurrence of the opiate pentapeptide /3-[D-Ala 2'4, Tyr 5] CM-5-NH2 in the... more In order to study the occurrence of the opiate pentapeptide /3-[D-Ala 2'4, Tyr 5] CM-5-NH2 in the 10 -9-10 -5 M concentration range, we have developed a new method consisting of extraction of the peptide from its buffered aqueous medium onto a reversed phase cartridge of 50 #L void volume, followed by on-line injection onto a Cu(ll) modified silica gel column. The acetonitrile-rich mobile phase allows detection of the natural fluorescence of the peptide at the picomole level. dent manner . This peptide acts, like enkephalins, in the 10-9-10 -5 M concentration range. In addition, it has been demonstrated that this peptide is able to cross intact the intestinal mucosa and thus is active from the luminal site of the intestine [8]. The classical RP HPLC method with UV detection at 214 nm previously used for the analysis of the peptide has a detection limit in the 0.1-1 nanomole range. For this reasons the determination of its metabolic fate and transport in vitro or in vivo needed the preconcentration of large volumes before injection in the chromatograph. In the present study, a method has been developed that permits the detection of the peptide in the picomole range. This technique combines a reversed phase preconcentration step to extract the peptide from the physiological medium, followed by ligand exchange chromatography and fluorescence detection at the picomole level; we show here that thiscombination is very powerful and gives reproducible results 9 1 Present address: c/o Pr. Nakanishi,
... 1. Separation of (dC, dT) (34,5,426o-units) on Nucleosil Cxs (7.5 #m) column (250 x 4.6 mm ID... more ... 1. Separation of (dC, dT) (34,5,426o-units) on Nucleosil Cxs (7.5 #m) column (250 x 4.6 mm ID) by isocratic elution with 86% A (A = 0.1 M ammonium acetate) and 14% B (B = methanol-water, 60:40) at room temperature; flow-rate, 1 ml/min. Peaks: I = (dC-dT); 2 = (dT-dC). Fig. ...
Revue des sciences de l'eau, 1999
The EMBO Journal, 1998
We report herein the isolation of ccf, a new gene located in region 82E and essential for Drosoph... more We report herein the isolation of ccf, a new gene located in region 82E and essential for Drosophila development. This gene, expressed throughout development, encodes a novel product of 68 kDa which is found in the nucleus during interphase and labels, in a novel pattern, centrosomes and chromosome arms during mitosis. Mutations in ccf give rise to late larvae with small imaginal discs and to adults showing appendages of reduced size, consistent with CCF involvement in cell proliferation. Neuroblast squash analyses show that CCF is required for proper condensation of mitotic chromosomes and, therefore, for progression through mitosis. Furthermore, we observe that adult ccf mutants as well as animals overexpressing CCF during larval stages exhibit homeotic transformations. We also find that mutations in the Pc-G genes Polycomb, polyhomeotic and Enhancer of zeste are enhanced by ccf mutations. Finally, we show that the CCF protein binds to specific sites on polytene chromosomes, many of which are shared with the Posterior sex combs Pc-G protein. Together, these results suggest a role for the CCF protein in the maintenance of chromosome structure during mitosis and interphase.
Tellus B, 1989
A three-dimensional mesoscale numerical model with detailed parameterized microphysics of clouds ... more A three-dimensional mesoscale numerical model with detailed parameterized microphysics of clouds and precipitation has been applied to the study of gas and aerosol wet removal. Twodimensional sensitivity tests combining meteorological predictions with pollutant scavenging and aqueous chemistry parameterizations have been carried out for continental and maritime clouds over an idealized topography. Model results indicate that nucleation scavenging is the most efficient in-cloud removal mechanism. Furthermore, differences in cloud droplet spectra in continental versus maritime cases, lead to different contributions of nucleation scavenging, in-solution oxidation of SO, by ozone and hydrogen peroxide, to wet sulfate deposition and acidity production. This results emphasizes the need for coupled treatment of dynamical, microphysical and chemical processes.
Tellus B, 2007
A B S T R A C T Few global aerosol models deal with size differentiated inorganic/organic particl... more A B S T R A C T Few global aerosol models deal with size differentiated inorganic/organic particles. Among them, still fewer ones explicitly treat secondary organic aerosol (SOA) formation. In this context, we have coupled the global chemistrytransport model (CTM) TM4 and the aerosol sectional model ORISAM (ORganic and Inorganic Sectional Aerosol Model, Bessagnet et al., 2002). This new aerosol model ORISAM-TM4 can accommodate aerosol size distributions with a variable number of diameter sections (bins) between 0.04 μm and over 10 μm and detailed organic/inorganic chemistry coupled with optional gas schemes. Two model versions are presented: a tracer version and a fully detailed eight-bin version with SOA formation. Focus is made on carbonaceous BC (black carbon) and OC (organic carbon) aerosols. First, significant developments both in ORISAM and in TM4 are discussed in line with the incorporation of updated emission inventories of BC and primary OC (OCp). Then, general comparisons are made between simulated BC and OC concentrations in air and precipitation against worldwide measurements. Also for BC, sensitivity tests using different updated fossil fuel emission inventories are focused over Europe, where emission controls make great strides. The tracer version appears generally satisfactory for BC mostly at background and remote sites, but not for total OC. For this latter, quite significant improvements result from the incorporation of SOA formation in ORISAM-TM4, instead of estimating OC as being simply proportional to OCp, as done in most existing models. Conclusions and prospects are then given.