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Papers by RUPAK CHOWDHURY

Research paper thumbnail of Liver and Cardiac Involvement in Shwachman-Diamond Syndrome: A Literature Review

Research paper thumbnail of Assessing the Key Attributes of Low Utilization of Mammography Screening and Breast-self Exam among African-American Women

Research paper thumbnail of Attenuation of Lipopolysaccharide Induced Cardiac Toxicity and the Role of Curcumin

Research paper thumbnail of Etiological Association Between Psoriasis and Thyroid Diseases

Research paper thumbnail of Research Article Curcumin Attenuation of Lipopolysaccharide Induced Cardiac Hypertrophy in Rodents

Copyright © 2013 Rupak Chowdhury et al. This is an open access article distributed under the Crea... more Copyright © 2013 Rupak Chowdhury et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100

Research paper thumbnail of Results and Discussion 3 . 1 . Curcumin Protects Mice against LPS Induced Cardiomeg

To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertr... more To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100 μg/kg of body weight by intraperitoneal route (IP), (C) LPS: 60mg/kg (IP), and (D) LPS + curcumin: both at previously stated concentrations by IP route. All mice were sacrificed as 12 hr and 24 hrs groups accordingly after LPS injection. The hearts were collected, photographed for cardiomegaly, and weighed to compare heart weight/brain weight (HW/BW) in mg/mg. For immunohistochemistry, the tissue sections were exposed to histone H3, H4 and acetylated histone H3, H4 antibody. LPS induced a significant increase in histone acetylation as shown by intense staining. In curcumin + LPS treated mice nuclear staining was similar to the control group indicating that curcumin traversed the histone acetylation activity of the LPS. To further check the mechanism of action of curcumin, p300 prote...

Research paper thumbnail of Attenuation of Lipopolysaccharide Induced Cardiac Toxicity and the Role of Curcumin

Research paper thumbnail of Liver and Cardiac Involvement in Shwachman-Diamond Syndrome: A Literature Review

Research paper thumbnail of Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation

Journal of nutrition & intermediary metabolism, 2018

Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potent... more Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and pro-inflammatory cytokine secretion to test the hypothesis that oral consumption of whole grape formulation reduces inflammatory signaling in the body. NF-κB luciferase reporter mice were divided into two groups, one which was fed an experimental diet formulated with 4% (w/w) whole grape powder (WGP) or another which was fed a control diet formulated with 3.6% glucose/fructose (w/w) combination. Simulated inflammation was induced in the mice by intraperitoneal injection of lipopolysaccharide (LPS).imaging was used to determine the effect of each diet on NF-κB activity. We found that there were no significant differences in weight gain between the WGP and control diet groups. However, there was a s...

Research paper thumbnail of Bromoethylindole (BEI-9) redirects NF-κB signaling induced by camptothecin and TNFα to promote cell death in colon cancer cells

Apoptosis : an international journal on programmed cell death, 2017

Chemotherapeutic regimens containing camptothecin (CPT), 5-fluorouracil, and oxaliplatin are used... more Chemotherapeutic regimens containing camptothecin (CPT), 5-fluorouracil, and oxaliplatin are used to treat advanced colorectal cancer. We previously reported that an indole derivative, 3-(2-bromoethyl)indole (BEI-9), inhibited the proliferation of colon cancer cells and suppressed NF-κB activation. Here, we show that a combination of BEI-9 with either CPT or tumor necrosis factor alpha (TNFα) enhances cell death. Using colorectal cancer cells, we examined the activation of NF-κB by drugs, the potential of BEI-9 for inhibiting drug-induced NF-κB activation, and the enhancement of cell death by combination treatments. Cells were treated with the chemotherapeutic drugs alone or in combination with BEI-9. NF-κB activation, cell cycle profiles, DNA-damage response, markers of cell death signaling and targets of NF-κB were evaluated to determine the effects of single and co-treatments. The combination of BEI-9 with CPT or TNFα inhibited NF-κB activation and reduced the expression of NF-κB...

Research paper thumbnail of Abstract A41: Development of a risk analysis model for triple-negative breast cancer stages and treatment of African American women

Clinical Cancer Research, 2017

The main object of this study is to assess how different stages of triple-negative breast cancer ... more The main object of this study is to assess how different stages of triple-negative breast cancer (TNBC) influences treatment of triple negative breast cancer patient. The data was gathered from Alabama Department of Public Health. We developed a probabilistic pathway scenario tree based on the knowledge from the comprehensive literature review and organized the variables into twelve major parameter categories. We performed Monte Carlo simulations with the software @Risk 5.7 (Palisade Corporation) for the quantitative risk assessment to capture the uncertainty for the treatment of triple negative breast cancer and different stages of TNBC. The likelihood (LH) of having one of the treatments for Stage IV TNBC, Surgery and or Chemotherapy (CT) or none gave that the person is (African American women) AAW older than 50, TNBC ranges from a min. of 1.62 × 10−3 to a max. of 2.35 × 10−2 with the mean and standard deviation of 8.28 × 10−3 and 3.141 × 10−3 respectively. The LH of having one of...

Research paper thumbnail of Assessing the Key Attributes of Low Utilization of Mammography Screening and Breast-self Exam among African-American Women

Journal of Cancer, 2016

African-American (AA) women living in four Black Belt Counties (BBC) of Alabama; consisting of Ba... more African-American (AA) women living in four Black Belt Counties (BBC) of Alabama; consisting of Barbour, Macon, Green and Wilcox are known to have lower mammogram utilization and breast self-exam rates when compared to their white female counterparts. The influence of socioeconomic and demographic factors on these disparities has not been clearly defined so far. Our study was designed to determine whether these observed disparities can be predicted with the socioeconomic and other demographic attributes. Health Disparity Questionnaires data (n = 516) for BBC of Alabama was analyzed using a logistic regression model to examine the association of breast cancer screening rates and breast self-exam with income, the level of education, family doctor, type of health insurance, obesity, and age. Income, education, family doctor, age and health insurance were independent predictors for the low utilization rate of mammography and breast self-exam (BSE). Improving socioeconomic conditions such...

Research paper thumbnail of Curcumin Attenuation of Lipopolysaccharide Induced Cardiac Hypertrophy in Rodents

ISRN Inflammation, 2013

To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertr... more To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100 μg/kg of body weight by intraperitoneal route (IP), (C) LPS: 60 mg/kg (IP), and (D) LPS + curcumin: both at previously stated concentrations by IP route. All mice were sacrificed as 12 hr and 24 hrs groups accordingly after LPS injection. The hearts were collected, photographed for cardiomegaly, and weighed to compare heart weight/brain weight (HW/BW) in mg/mg. For immunohistochemistry, the tissue sections were exposed to histone H3, H4 and acetylated histone H3, H4 antibody. LPS induced a significant increase in histone acetylation as shown by intense staining. In curcumin + LPS treated mice nuclear staining was similar to the control group indicating that curcumin traversed the histone acetylation activity of the LPS. To further check the mechanism of action of curcumin, p300 prot...

Research paper thumbnail of Antimicrobial Activity of Curcumin Against Food-Borne Pathogens

Vedic Research International Biological Medicinal Chemistry, 2014

Pre and post-harvest practices are very important factors in controlling Salmonella and Escherich... more Pre and post-harvest practices are very important factors in controlling Salmonella and Escherichia coli food pathogens in poultry meat. Both these pre-harvest and postharvest practices are controversial and objectionable due to health hazards and allergies associated with them. Ideally, a novel and safer compounds that could be used both in preand post-harvest pathogen reduction strategies are needed. Hence our hypothesis tested namely ‘Curcumin, a natural product, may replace the controversial substances currently used in pre-harvest and post-harvest practices for controlling food pathogens like Salmonella and Escherichia coli.’ The purpose of this study was to determine the effects of Curcumin on the growth of Salmonella, E. coli, macrophage uptake of bacteria, and on bacterial ATPase activity using the BacTiter-Glotm Cell Viability Assay. This study also evaluated the In vitro effect of Curcumin on macrophage phagocytosis. Data from this study showed that growth percentages of Salmonella and Escherichia coli were inhibited with increasing doses of Curcumin. Our molecular mechanism of action investigations of Curcumin determined that the amount of ATP present in both Salmonella and Escherichia coli was reduced after treatment with Curcumin. In summary, these data demonstrates promising potential of Curcumin as a universally acceptable pre-harvest and post-harvest alternative. Antimicrobial Activity of Curcumin Against Food-Borne Pathogens Food poisoning is an illness caused by eating foods that contain harmful organisms. These harmful germs can include bacteria, parasites, and viruses. They are mostly found in raw meat, chicken, fish, and eggs, with potential to spread to any type of food. It is normal to find bacteria in the intestines of healthy animals used for food. If bacteria come in contact with meat or poultry during processing, they can contaminate the food. Campylobacter, Salmonella, and E.coli are important bacteria that cause food poisoning [1]. Salmonellosis is a type of food poisoning caused by the Page No 12 Journal home page at www.VedicJournals.com eISSN 2330-7250 Research Article DOI: http://dx.doi.org/10.14259/bmc.v2i1.102 Article Info: Received: January 14th, 2014; Accepted: February 2nd, 2014

Research paper thumbnail of Abstract 3783: Co-treatment of metastasis-derived colon cancer cells with bromoethyl indole (BEI) enhances camptothecin or TNF alpha-induced cell death

Cancer Research, 2016

Colorectal cancer is still the third most deadly cancer in the United States. Currently, there ar... more Colorectal cancer is still the third most deadly cancer in the United States. Currently, there are limited choices of targeted anticancer agents that are durably effective against colorectal cancer. Therefore, chemotherapeutic regimens containing the drugs Camptothecin (CPT), 5-FU and Oxaliplatin remain the mainstays for the treatment of advanced colorectal cancer. We recently found that the indole-derivative small compound Bromoethyl Indole (BEI) potently inhibits the proliferation of colon cancer cells and also suppresses NF-kB activation. In this study, we investigated if the combination of BEI with either CPT or TNFα would enhance cell death in vitro. Metastasis-derived parental or engineered NF-kB-reporter colon cancer cells were used to examine 1) the activation of NF-kB signaling by clinically used drugs, 2) the potential for BEI to inhibit drug-induced NF-kB activation, and 3) the potential for combination treatment to enhance cell death in vitro. Cells were treated with eit...

Research paper thumbnail of Liver and Cardiac Involvement in Shwachman-Diamond Syndrome: A Literature Review

Research paper thumbnail of Assessing the Key Attributes of Low Utilization of Mammography Screening and Breast-self Exam among African-American Women

Research paper thumbnail of Attenuation of Lipopolysaccharide Induced Cardiac Toxicity and the Role of Curcumin

Research paper thumbnail of Etiological Association Between Psoriasis and Thyroid Diseases

Research paper thumbnail of Research Article Curcumin Attenuation of Lipopolysaccharide Induced Cardiac Hypertrophy in Rodents

Copyright © 2013 Rupak Chowdhury et al. This is an open access article distributed under the Crea... more Copyright © 2013 Rupak Chowdhury et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100

Research paper thumbnail of Results and Discussion 3 . 1 . Curcumin Protects Mice against LPS Induced Cardiomeg

To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertr... more To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100 μg/kg of body weight by intraperitoneal route (IP), (C) LPS: 60mg/kg (IP), and (D) LPS + curcumin: both at previously stated concentrations by IP route. All mice were sacrificed as 12 hr and 24 hrs groups accordingly after LPS injection. The hearts were collected, photographed for cardiomegaly, and weighed to compare heart weight/brain weight (HW/BW) in mg/mg. For immunohistochemistry, the tissue sections were exposed to histone H3, H4 and acetylated histone H3, H4 antibody. LPS induced a significant increase in histone acetylation as shown by intense staining. In curcumin + LPS treated mice nuclear staining was similar to the control group indicating that curcumin traversed the histone acetylation activity of the LPS. To further check the mechanism of action of curcumin, p300 prote...

Research paper thumbnail of Attenuation of Lipopolysaccharide Induced Cardiac Toxicity and the Role of Curcumin

Research paper thumbnail of Liver and Cardiac Involvement in Shwachman-Diamond Syndrome: A Literature Review

Research paper thumbnail of Effects of consumption of whole grape powder on basal NF-κB signaling and inflammatory cytokine secretion in a mouse model of inflammation

Journal of nutrition & intermediary metabolism, 2018

Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potent... more Dietary consumption of polyphenol-rich fruits, such as grapes, may reduce inflammation and potentially prevent diseases linked to inflammation. Here, we used a genetically engineered murine model to measure Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) activity and pro-inflammatory cytokine secretion to test the hypothesis that oral consumption of whole grape formulation reduces inflammatory signaling in the body. NF-κB luciferase reporter mice were divided into two groups, one which was fed an experimental diet formulated with 4% (w/w) whole grape powder (WGP) or another which was fed a control diet formulated with 3.6% glucose/fructose (w/w) combination. Simulated inflammation was induced in the mice by intraperitoneal injection of lipopolysaccharide (LPS).imaging was used to determine the effect of each diet on NF-κB activity. We found that there were no significant differences in weight gain between the WGP and control diet groups. However, there was a s...

Research paper thumbnail of Bromoethylindole (BEI-9) redirects NF-κB signaling induced by camptothecin and TNFα to promote cell death in colon cancer cells

Apoptosis : an international journal on programmed cell death, 2017

Chemotherapeutic regimens containing camptothecin (CPT), 5-fluorouracil, and oxaliplatin are used... more Chemotherapeutic regimens containing camptothecin (CPT), 5-fluorouracil, and oxaliplatin are used to treat advanced colorectal cancer. We previously reported that an indole derivative, 3-(2-bromoethyl)indole (BEI-9), inhibited the proliferation of colon cancer cells and suppressed NF-κB activation. Here, we show that a combination of BEI-9 with either CPT or tumor necrosis factor alpha (TNFα) enhances cell death. Using colorectal cancer cells, we examined the activation of NF-κB by drugs, the potential of BEI-9 for inhibiting drug-induced NF-κB activation, and the enhancement of cell death by combination treatments. Cells were treated with the chemotherapeutic drugs alone or in combination with BEI-9. NF-κB activation, cell cycle profiles, DNA-damage response, markers of cell death signaling and targets of NF-κB were evaluated to determine the effects of single and co-treatments. The combination of BEI-9 with CPT or TNFα inhibited NF-κB activation and reduced the expression of NF-κB...

Research paper thumbnail of Abstract A41: Development of a risk analysis model for triple-negative breast cancer stages and treatment of African American women

Clinical Cancer Research, 2017

The main object of this study is to assess how different stages of triple-negative breast cancer ... more The main object of this study is to assess how different stages of triple-negative breast cancer (TNBC) influences treatment of triple negative breast cancer patient. The data was gathered from Alabama Department of Public Health. We developed a probabilistic pathway scenario tree based on the knowledge from the comprehensive literature review and organized the variables into twelve major parameter categories. We performed Monte Carlo simulations with the software @Risk 5.7 (Palisade Corporation) for the quantitative risk assessment to capture the uncertainty for the treatment of triple negative breast cancer and different stages of TNBC. The likelihood (LH) of having one of the treatments for Stage IV TNBC, Surgery and or Chemotherapy (CT) or none gave that the person is (African American women) AAW older than 50, TNBC ranges from a min. of 1.62 × 10−3 to a max. of 2.35 × 10−2 with the mean and standard deviation of 8.28 × 10−3 and 3.141 × 10−3 respectively. The LH of having one of...

Research paper thumbnail of Assessing the Key Attributes of Low Utilization of Mammography Screening and Breast-self Exam among African-American Women

Journal of Cancer, 2016

African-American (AA) women living in four Black Belt Counties (BBC) of Alabama; consisting of Ba... more African-American (AA) women living in four Black Belt Counties (BBC) of Alabama; consisting of Barbour, Macon, Green and Wilcox are known to have lower mammogram utilization and breast self-exam rates when compared to their white female counterparts. The influence of socioeconomic and demographic factors on these disparities has not been clearly defined so far. Our study was designed to determine whether these observed disparities can be predicted with the socioeconomic and other demographic attributes. Health Disparity Questionnaires data (n = 516) for BBC of Alabama was analyzed using a logistic regression model to examine the association of breast cancer screening rates and breast self-exam with income, the level of education, family doctor, type of health insurance, obesity, and age. Income, education, family doctor, age and health insurance were independent predictors for the low utilization rate of mammography and breast self-exam (BSE). Improving socioeconomic conditions such...

Research paper thumbnail of Curcumin Attenuation of Lipopolysaccharide Induced Cardiac Hypertrophy in Rodents

ISRN Inflammation, 2013

To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertr... more To study the ameliorating effects of curcumin in lipopolysaccharide (LPS) induced cardiac hypertrophy, mice were assigned to 4 groups (3 males and 3 females in each group): (A) control, (B) curcumin: 100 μg/kg of body weight by intraperitoneal route (IP), (C) LPS: 60 mg/kg (IP), and (D) LPS + curcumin: both at previously stated concentrations by IP route. All mice were sacrificed as 12 hr and 24 hrs groups accordingly after LPS injection. The hearts were collected, photographed for cardiomegaly, and weighed to compare heart weight/brain weight (HW/BW) in mg/mg. For immunohistochemistry, the tissue sections were exposed to histone H3, H4 and acetylated histone H3, H4 antibody. LPS induced a significant increase in histone acetylation as shown by intense staining. In curcumin + LPS treated mice nuclear staining was similar to the control group indicating that curcumin traversed the histone acetylation activity of the LPS. To further check the mechanism of action of curcumin, p300 prot...

Research paper thumbnail of Antimicrobial Activity of Curcumin Against Food-Borne Pathogens

Vedic Research International Biological Medicinal Chemistry, 2014

Pre and post-harvest practices are very important factors in controlling Salmonella and Escherich... more Pre and post-harvest practices are very important factors in controlling Salmonella and Escherichia coli food pathogens in poultry meat. Both these pre-harvest and postharvest practices are controversial and objectionable due to health hazards and allergies associated with them. Ideally, a novel and safer compounds that could be used both in preand post-harvest pathogen reduction strategies are needed. Hence our hypothesis tested namely ‘Curcumin, a natural product, may replace the controversial substances currently used in pre-harvest and post-harvest practices for controlling food pathogens like Salmonella and Escherichia coli.’ The purpose of this study was to determine the effects of Curcumin on the growth of Salmonella, E. coli, macrophage uptake of bacteria, and on bacterial ATPase activity using the BacTiter-Glotm Cell Viability Assay. This study also evaluated the In vitro effect of Curcumin on macrophage phagocytosis. Data from this study showed that growth percentages of Salmonella and Escherichia coli were inhibited with increasing doses of Curcumin. Our molecular mechanism of action investigations of Curcumin determined that the amount of ATP present in both Salmonella and Escherichia coli was reduced after treatment with Curcumin. In summary, these data demonstrates promising potential of Curcumin as a universally acceptable pre-harvest and post-harvest alternative. Antimicrobial Activity of Curcumin Against Food-Borne Pathogens Food poisoning is an illness caused by eating foods that contain harmful organisms. These harmful germs can include bacteria, parasites, and viruses. They are mostly found in raw meat, chicken, fish, and eggs, with potential to spread to any type of food. It is normal to find bacteria in the intestines of healthy animals used for food. If bacteria come in contact with meat or poultry during processing, they can contaminate the food. Campylobacter, Salmonella, and E.coli are important bacteria that cause food poisoning [1]. Salmonellosis is a type of food poisoning caused by the Page No 12 Journal home page at www.VedicJournals.com eISSN 2330-7250 Research Article DOI: http://dx.doi.org/10.14259/bmc.v2i1.102 Article Info: Received: January 14th, 2014; Accepted: February 2nd, 2014

Research paper thumbnail of Abstract 3783: Co-treatment of metastasis-derived colon cancer cells with bromoethyl indole (BEI) enhances camptothecin or TNF alpha-induced cell death

Cancer Research, 2016

Colorectal cancer is still the third most deadly cancer in the United States. Currently, there ar... more Colorectal cancer is still the third most deadly cancer in the United States. Currently, there are limited choices of targeted anticancer agents that are durably effective against colorectal cancer. Therefore, chemotherapeutic regimens containing the drugs Camptothecin (CPT), 5-FU and Oxaliplatin remain the mainstays for the treatment of advanced colorectal cancer. We recently found that the indole-derivative small compound Bromoethyl Indole (BEI) potently inhibits the proliferation of colon cancer cells and also suppresses NF-kB activation. In this study, we investigated if the combination of BEI with either CPT or TNFα would enhance cell death in vitro. Metastasis-derived parental or engineered NF-kB-reporter colon cancer cells were used to examine 1) the activation of NF-kB signaling by clinically used drugs, 2) the potential for BEI to inhibit drug-induced NF-kB activation, and 3) the potential for combination treatment to enhance cell death in vitro. Cells were treated with eit...