Rafael Botero - Academia.edu (original) (raw)
Papers by Rafael Botero
Journal of Experimental & Clinical Cancer Research, 2008
Background: alpha-Methylacyl-CoA racemase (AMACR), an immunomarker for prostatic adenocarcinoma, ... more Background: alpha-Methylacyl-CoA racemase (AMACR), an immunomarker for prostatic adenocarcinoma, has been shown to be expressed in a variety of other neoplasms. This study aims to evaluate immunohistochemical expression of the AMACR in neoplastic and nonneoplastic liver lesions, and assess its value in the diagnosis of hepatocellular carcinoma (HCC). Methods: Formalin-fixed paraffin-embedded tissue sections of 51 HCC (14 well, 22 moderately and 15 poorly differentiated), 9 hepatocellular adenoma (HCA), 48 cirrhotic nodules (CN) and 16 normal liver tissues (NLT) were immunostained for AMACR. Results: Expression of AMACR is significantly enhanced in HCC tissue compared with non-HCC tissue. High expression of AMACR was found in 82% of HCC including 86% of well-differentiated HCC. In contrast, only 11% of HCA, 13% of CN and 6% of NLT showed high expression for AMACR. Clinicopathological evaluation showed a significant correlation between AMACR expression and venous invasion and capsular invasion by HCC. Conclusion: Our results suggest that AMACR staining may serve as a useful marker for the differential diagnosis of well-differentiated HCC from HCA. Increased AMACR expression and its association with tumor venous invasion suggest that AMACR may play a role in HCC development and progression.
European Journal of Gastroenterology & Hepatology, 2008
Drug hepatotoxicity is the most common cause of fulminant hepatic failure in the USA. We describe... more Drug hepatotoxicity is the most common cause of fulminant hepatic failure in the USA. We describe a rare case of a patient who developed an acute liver injury after initiation of therapy with quetiapine, but after conservative management and a trial of steroids, has fully recovered. This is the second reported case of quetiapine-induced liver injury in the published literature.
Con el fin de establecer el valor diagnostico de los acidos biliares sericos se estudiaron 81 pac... more Con el fin de establecer el valor diagnostico de los acidos biliares sericos se estudiaron 81 pacientes, atendidos por el Instituto de Enfermedades Hepatobiliares de la Fundacion Santa Fe de Bogota (IEHB). Encontramos que la sensibilidad de la coliglicina (CG) RIA (un acido biliar) para detectar enfermedad hepatobiliar fue similar a la de la AST y la fosfatasa alcalina (78.5%, 81.5% y 78.5%, respectivamente), y superior a la de las otras pruebas (ALT y bilirrubina total). La especificidad (100%) y los valores predictivos (prueba positiva 100%, prueba negativa 54.3%) fueron mejores que los de las pruebas convencionales (p<0.05). El perfil hepatico (AST, ALT, fosfatasa alcalina y bilirrubina total) tuvo alta sensibilidad (95.4%), pero muy baja especificidad (43.8%). La CG RIA fue positiva en cirroticos un mayor numero de veces que las otras pruebas (95.2%), pero no encontramos que los niveles de CG RIA se correlacionaran con el estado clinico de la cirrosis segun la clasificacion d...
Clinical Liver Disease, 2013
American Journal of Gastroenterology
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Cholangiocarcinoma represents 10% of primary liver malignancies and accounts for less than 3% of ... more Cholangiocarcinoma represents 10% of primary liver malignancies and accounts for less than 3% of all gastrointestinal malignant tumors, with an enormous geographical variation. This neoplasia can arise from the biliary tract epithelium or hepatic progenitor cells. Depending on the anatomic localization, it is classified into three subtypes: intrahepatic, perihilar and distal. This fact is one of the main difficulties, because there are many studies that indistinctly include the results in the management of these different types of cholangiocarcinoma, without differentiating its location and even including gallbladder cancer. There are many controversial points in epidemiology, liver transplantation as a treatment, limitations of different results by group and type of treatment, histological testing and chemotherapy. This is a narrative review about topics in cholangiocarcinoma. This article is part of a Special Issue entitled: Cholangiocytes in Health and Disease edited by Jesus Banales, Marco Marzioni, Nicholas LaRusso and Peter Jansen.
Revista Colombiana De Gastroenterologia, Sep 1, 2013
Acta Medica Colombiana, Apr 1, 2013
Acta Medica Colombiana Amc Organo De La Asociacion Columbiana De Medicina Interna, Apr 1, 2013
Revista Colombiana De Gastroenterologia, Sep 1, 2013
Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto
Clinical Liver Disease, 2015
Clinical Liver Disease, 2015
Transplantation proceedings, 2004
Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and... more Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and 2 to 3 million Americans. End-stage liver disease secondary to chronic HCV infection is the most frequent indication for liver transplantation in this country. Currently, the gold standard for treatment for immunocompetent patients is a combination of peginterferon (PEG-IFN) and ribavirin for 6 to 12 months depending on the genotype. This treatment achieves a sustained virological response (SVR) in 54% to 61% of patients overall. Almost 50% of patients do not respond or have recurrences posttreatment and progress in over 10 to 20 years into chronic liver disease and its complications. Liver transplantation is the only therapeutic modality that impacts on quality of life and survival of these patients. However, recurrence of HCV in the new allograft is universal with accelerated progression to cirrhosis in 5 to 10 years. Response to treatment is usually low (20% to 30%), and associated w...
Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and... more Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and 2 to 3 million Americans. End-stage liver disease secondary to chronic HCV infection is the most frequent indication for liver transplantation in this country. Currently, the gold standard for treatment for immunocompetent patients is a combination of peginterferon (PEG-IFN) and ribavirin for 6 to 12 months depending on the genotype. This treatment achieves a sustained virological response (SVR) in 54% to 61% of patients overall. Almost 50% of patients do not respond or have recurrences posttreatment and progress in over 10 to 20 years into chronic liver disease and its complications. Liver transplantation is the only therapeutic modality that impacts on quality of life and survival of these patients. However, recurrence of HCV in the new allograft is universal with accelerated progression to cirrhosis in 5 to 10 years. Response to treatment is usually low (20% to 30%), and associated with significant side effects and depression. A significant percentage of patients with recurrent HCV after transplantation require retransplantation to control the complications of end-stage liver disease. Other solid organ transplants recipients already HCV-positive, or infected at the time of transplantation from blood transfusions or an infected graft, develop accelerated, progressive liver disease facilitated by the adverse effects of immunosuppression in addition to HCV replication. To prevent morbidity, mortality, and high costs related to the consequences of HCV infection, all solid organ transplant candidates should be tested for HCV infection and treated appropriately with PEG-IFN and ribavirin prior to transplantation.
Gastroenterología y Hepatología, 2004
ABSTRACT
Clinics in Liver Disease, 2003
The discovery of a single test of liver function has been a goal of hepatologists for many years.... more The discovery of a single test of liver function has been a goal of hepatologists for many years. The great complexity of the liver and its many diverse functions, however, has prevented such an accomplishment. An analogy can be made with the way one currently uses liver tests where several individual tests are combined into a profile. This article presents evidence that confirms the same concept: Only by combining several clinical and laboratory measures can we predict the prognosis of liver disease patients. End-stage liver disease and pediatric end-stage liver disease models are valuable additions to the prognostic armamentarium; however, these models are not perfect and some important indications for liver transplant today cannot be included because their main issue is not disease severity.
Transplantation Proceedings, 2005
Chronic infection with hepatitis B virus (HBV) is one of the most common causes of cirrhosis of t... more Chronic infection with hepatitis B virus (HBV) is one of the most common causes of cirrhosis of the liver and hepatocellular carcinoma worldwide, frequently requiring liver transplantation. Other nonliver organ transplants get infected de novo or through reactivation from previous active or inactive infections. With significant improvements in the surgical techniques and immunosuppressive regimens over the last 20 years, organ transplantation has become the most effective and lifesaving therapy for patients with chronic renal failure, cirrhosis, hepatocarcinoma, and heart failure. Until recently chronic HBV infection was considered a formal contraindication for liver transplantation, since recurrence of infection without prophylaxis occurs in 75% to 90% of the patients, with significant morbidity and mortality and few therapeutic alternatives. However, the introduction of hepatitis B immunoglobulin (HBIG) a decade ago to reduce the risk of reinfection of liver grafts, and more recently the availability of nucleoside analogues with few side effects and easy administration, have led to a dramatic improvement in patient outcomes with a risk of long-term HBV reinfection of less than 10% with combined HBIG and lamivudine prophylaxis. Chronic HBV infection in kidney, heart, and other organs has become a serious long-term problem and one of the most frequent and important comorbidities affecting graft and patient survival. Fortunately the introduction of nucleoside analogues allows significant control of viral replication and prevents progression of liver disease and other organ damage. In the present article we discuss the current indications for HBV prophylaxis and treatment prior to and after organ transplantation, as well as the most cost-effective way to apply different regimens to reduce side effects and improve survival and quality of life after transplantation.
Clinics in liver disease, 2003
The discovery of a single test of liver function has been a goal of hepatologists for many years.... more The discovery of a single test of liver function has been a goal of hepatologists for many years. The great complexity of the liver and its many diverse functions, however, has prevented such an accomplishment. An analogy can be made with the way one currently uses liver tests where several individual tests are combined into a profile. This article presents evidence that confirms the same concept: Only by combining several clinical and laboratory measures can we predict the prognosis of liver disease patients. End-stage liver disease and pediatric end-stage liver disease models are valuable additions to the prognostic armamentarium; however, these models are not perfect and some important indications for liver transplant today cannot be included because their main issue is not disease severity.
Journal of Experimental & Clinical Cancer Research, 2008
Background: alpha-Methylacyl-CoA racemase (AMACR), an immunomarker for prostatic adenocarcinoma, ... more Background: alpha-Methylacyl-CoA racemase (AMACR), an immunomarker for prostatic adenocarcinoma, has been shown to be expressed in a variety of other neoplasms. This study aims to evaluate immunohistochemical expression of the AMACR in neoplastic and nonneoplastic liver lesions, and assess its value in the diagnosis of hepatocellular carcinoma (HCC). Methods: Formalin-fixed paraffin-embedded tissue sections of 51 HCC (14 well, 22 moderately and 15 poorly differentiated), 9 hepatocellular adenoma (HCA), 48 cirrhotic nodules (CN) and 16 normal liver tissues (NLT) were immunostained for AMACR. Results: Expression of AMACR is significantly enhanced in HCC tissue compared with non-HCC tissue. High expression of AMACR was found in 82% of HCC including 86% of well-differentiated HCC. In contrast, only 11% of HCA, 13% of CN and 6% of NLT showed high expression for AMACR. Clinicopathological evaluation showed a significant correlation between AMACR expression and venous invasion and capsular invasion by HCC. Conclusion: Our results suggest that AMACR staining may serve as a useful marker for the differential diagnosis of well-differentiated HCC from HCA. Increased AMACR expression and its association with tumor venous invasion suggest that AMACR may play a role in HCC development and progression.
European Journal of Gastroenterology & Hepatology, 2008
Drug hepatotoxicity is the most common cause of fulminant hepatic failure in the USA. We describe... more Drug hepatotoxicity is the most common cause of fulminant hepatic failure in the USA. We describe a rare case of a patient who developed an acute liver injury after initiation of therapy with quetiapine, but after conservative management and a trial of steroids, has fully recovered. This is the second reported case of quetiapine-induced liver injury in the published literature.
Con el fin de establecer el valor diagnostico de los acidos biliares sericos se estudiaron 81 pac... more Con el fin de establecer el valor diagnostico de los acidos biliares sericos se estudiaron 81 pacientes, atendidos por el Instituto de Enfermedades Hepatobiliares de la Fundacion Santa Fe de Bogota (IEHB). Encontramos que la sensibilidad de la coliglicina (CG) RIA (un acido biliar) para detectar enfermedad hepatobiliar fue similar a la de la AST y la fosfatasa alcalina (78.5%, 81.5% y 78.5%, respectivamente), y superior a la de las otras pruebas (ALT y bilirrubina total). La especificidad (100%) y los valores predictivos (prueba positiva 100%, prueba negativa 54.3%) fueron mejores que los de las pruebas convencionales (p<0.05). El perfil hepatico (AST, ALT, fosfatasa alcalina y bilirrubina total) tuvo alta sensibilidad (95.4%), pero muy baja especificidad (43.8%). La CG RIA fue positiva en cirroticos un mayor numero de veces que las otras pruebas (95.2%), pero no encontramos que los niveles de CG RIA se correlacionaran con el estado clinico de la cirrosis segun la clasificacion d...
Clinical Liver Disease, 2013
American Journal of Gastroenterology
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Cholangiocarcinoma represents 10% of primary liver malignancies and accounts for less than 3% of ... more Cholangiocarcinoma represents 10% of primary liver malignancies and accounts for less than 3% of all gastrointestinal malignant tumors, with an enormous geographical variation. This neoplasia can arise from the biliary tract epithelium or hepatic progenitor cells. Depending on the anatomic localization, it is classified into three subtypes: intrahepatic, perihilar and distal. This fact is one of the main difficulties, because there are many studies that indistinctly include the results in the management of these different types of cholangiocarcinoma, without differentiating its location and even including gallbladder cancer. There are many controversial points in epidemiology, liver transplantation as a treatment, limitations of different results by group and type of treatment, histological testing and chemotherapy. This is a narrative review about topics in cholangiocarcinoma. This article is part of a Special Issue entitled: Cholangiocytes in Health and Disease edited by Jesus Banales, Marco Marzioni, Nicholas LaRusso and Peter Jansen.
Revista Colombiana De Gastroenterologia, Sep 1, 2013
Acta Medica Colombiana, Apr 1, 2013
Acta Medica Colombiana Amc Organo De La Asociacion Columbiana De Medicina Interna, Apr 1, 2013
Revista Colombiana De Gastroenterologia, Sep 1, 2013
Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto
Clinical Liver Disease, 2015
Clinical Liver Disease, 2015
Transplantation proceedings, 2004
Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and... more Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and 2 to 3 million Americans. End-stage liver disease secondary to chronic HCV infection is the most frequent indication for liver transplantation in this country. Currently, the gold standard for treatment for immunocompetent patients is a combination of peginterferon (PEG-IFN) and ribavirin for 6 to 12 months depending on the genotype. This treatment achieves a sustained virological response (SVR) in 54% to 61% of patients overall. Almost 50% of patients do not respond or have recurrences posttreatment and progress in over 10 to 20 years into chronic liver disease and its complications. Liver transplantation is the only therapeutic modality that impacts on quality of life and survival of these patients. However, recurrence of HCV in the new allograft is universal with accelerated progression to cirrhosis in 5 to 10 years. Response to treatment is usually low (20% to 30%), and associated w...
Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and... more Chronic hepatitis C (HCV) infection affects more than 170 million people throughout the world and 2 to 3 million Americans. End-stage liver disease secondary to chronic HCV infection is the most frequent indication for liver transplantation in this country. Currently, the gold standard for treatment for immunocompetent patients is a combination of peginterferon (PEG-IFN) and ribavirin for 6 to 12 months depending on the genotype. This treatment achieves a sustained virological response (SVR) in 54% to 61% of patients overall. Almost 50% of patients do not respond or have recurrences posttreatment and progress in over 10 to 20 years into chronic liver disease and its complications. Liver transplantation is the only therapeutic modality that impacts on quality of life and survival of these patients. However, recurrence of HCV in the new allograft is universal with accelerated progression to cirrhosis in 5 to 10 years. Response to treatment is usually low (20% to 30%), and associated with significant side effects and depression. A significant percentage of patients with recurrent HCV after transplantation require retransplantation to control the complications of end-stage liver disease. Other solid organ transplants recipients already HCV-positive, or infected at the time of transplantation from blood transfusions or an infected graft, develop accelerated, progressive liver disease facilitated by the adverse effects of immunosuppression in addition to HCV replication. To prevent morbidity, mortality, and high costs related to the consequences of HCV infection, all solid organ transplant candidates should be tested for HCV infection and treated appropriately with PEG-IFN and ribavirin prior to transplantation.
Gastroenterología y Hepatología, 2004
ABSTRACT
Clinics in Liver Disease, 2003
The discovery of a single test of liver function has been a goal of hepatologists for many years.... more The discovery of a single test of liver function has been a goal of hepatologists for many years. The great complexity of the liver and its many diverse functions, however, has prevented such an accomplishment. An analogy can be made with the way one currently uses liver tests where several individual tests are combined into a profile. This article presents evidence that confirms the same concept: Only by combining several clinical and laboratory measures can we predict the prognosis of liver disease patients. End-stage liver disease and pediatric end-stage liver disease models are valuable additions to the prognostic armamentarium; however, these models are not perfect and some important indications for liver transplant today cannot be included because their main issue is not disease severity.
Transplantation Proceedings, 2005
Chronic infection with hepatitis B virus (HBV) is one of the most common causes of cirrhosis of t... more Chronic infection with hepatitis B virus (HBV) is one of the most common causes of cirrhosis of the liver and hepatocellular carcinoma worldwide, frequently requiring liver transplantation. Other nonliver organ transplants get infected de novo or through reactivation from previous active or inactive infections. With significant improvements in the surgical techniques and immunosuppressive regimens over the last 20 years, organ transplantation has become the most effective and lifesaving therapy for patients with chronic renal failure, cirrhosis, hepatocarcinoma, and heart failure. Until recently chronic HBV infection was considered a formal contraindication for liver transplantation, since recurrence of infection without prophylaxis occurs in 75% to 90% of the patients, with significant morbidity and mortality and few therapeutic alternatives. However, the introduction of hepatitis B immunoglobulin (HBIG) a decade ago to reduce the risk of reinfection of liver grafts, and more recently the availability of nucleoside analogues with few side effects and easy administration, have led to a dramatic improvement in patient outcomes with a risk of long-term HBV reinfection of less than 10% with combined HBIG and lamivudine prophylaxis. Chronic HBV infection in kidney, heart, and other organs has become a serious long-term problem and one of the most frequent and important comorbidities affecting graft and patient survival. Fortunately the introduction of nucleoside analogues allows significant control of viral replication and prevents progression of liver disease and other organ damage. In the present article we discuss the current indications for HBV prophylaxis and treatment prior to and after organ transplantation, as well as the most cost-effective way to apply different regimens to reduce side effects and improve survival and quality of life after transplantation.
Clinics in liver disease, 2003
The discovery of a single test of liver function has been a goal of hepatologists for many years.... more The discovery of a single test of liver function has been a goal of hepatologists for many years. The great complexity of the liver and its many diverse functions, however, has prevented such an accomplishment. An analogy can be made with the way one currently uses liver tests where several individual tests are combined into a profile. This article presents evidence that confirms the same concept: Only by combining several clinical and laboratory measures can we predict the prognosis of liver disease patients. End-stage liver disease and pediatric end-stage liver disease models are valuable additions to the prognostic armamentarium; however, these models are not perfect and some important indications for liver transplant today cannot be included because their main issue is not disease severity.