Rahul Sinha - Academia.edu (original) (raw)

Papers by Rahul Sinha

Research paper thumbnail of Tuning Cytokine Receptor Signaling by Re-orienting Dimer Geometry with Surrogate Ligands

Cell, Jan 12, 2015

Most cell-surface receptors for cytokines and growth factors signal as dimers, but it is unclear ... more Most cell-surface receptors for cytokines and growth factors signal as dimers, but it is unclear whether remodeling receptor dimer topology is a viable strategy to ''tune'' signaling output. We utilized diabodies (DA) as surrogate ligands in a prototypical dimeric receptor-ligand system, the cytokine Erythropoietin (EPO) and its receptor (EpoR), to dimerize EpoR ectodomains in non-native architectures. Diabodyinduced signaling amplitudes varied from full to minimal agonism, and structures of these DA/EpoR complexes differed in EpoR dimer orientation and proximity. Diabodies also elicited biased or differential activation of signaling pathways and gene expression profiles compared to EPO. Non-signaling diabodies inhibited proliferation of erythroid precursors from patients with a myeloproliferative neoplasm due to a constitutively active JAK2V617F mutation. Thus, intracellular oncogenic mutations causing ligand-independent receptor activation can be counteracted by extracellular ligands that reorient receptors into inactive dimer topologies. This approach has broad applications for tuning signaling output for many dimeric receptor systems.

Research paper thumbnail of Has new physics already been seen in B(d) meson decays?

Physical review letters, Jan 29, 2006

We show using a model independent approach that the deviation in measured B 0 d − B 0 d mixing ph... more We show using a model independent approach that the deviation in measured B 0 d − B 0 d mixing phase caused by pollution from another amplitude, within the Standard Model, is always less in magnitude, and has the same sign as the weak phase of the polluting amplitude. The exception is to have large destructive interference between the two amplitudes. We demonstrate that any deviation larger than a few degrees is only possible if the observed decay rate results from fine tuned cancellations between significantly larger quark level amplitudes. These simple observations have very significant consequences for signals of New Physics.

Research paper thumbnail of Urethral fistula following circumcision: salvaged by buccal mucosa graft urethroplasty

The Indian journal of surgery, 2009

Fistula following circumcision and at times accompanied by disfi gurement of the glans penis is a... more Fistula following circumcision and at times accompanied by disfi gurement of the glans penis is a common problem in our country, where a large number of circumcision is performed by untrained professionals. These complications may have profound negative psychological impact on the growing child. Herein, we report the successful closure of such fi stula using buccal mucosa, which occurred following circumcision (for phimosis) in a 15 year old boy. This resulted in the disfi gurement of the glans penis with a distal urethral fi stula. The fi stula was managed by dorsal onlay buccal mucosa graft urethroplasty (BMGU). The patient has excellent cosmesis at 6 months followup with a good urinary stream. This technique can give better results than those, where skin grafts or local fl aps are used for fi stula closure. BMGU is a versatile technique with good long term results. The cosmesis and functional results are satisfactory and help in improving the self esteem and sexual function in the years to come.

Research paper thumbnail of Subacute intestinal obstruction associated with a case of polycythemia vera--a rare combination

Subacute intestinal obstruction associated with a case of polycythemia vera--a rare combination

JPMA. The Journal of the Pakistan Medical Association, 2009

Primary Polycythemia Vera (PV) is difficult to diagnose due to its varied and atypical 1 presenta... more Primary Polycythemia Vera (PV) is difficult to diagnose due to its varied and atypical 1 presentation. It is a myeloproliferative disorder which is managed conservatively. We submit a case report of a patient who was admitted in surgery with subacute intestinal obstruction due to compression by a massive splenic cyst. The investigations showed PV causing splenomegaly.

Research paper thumbnail of Determination of weak amplitudes using Bose symmetry and Dalitz plots

Physical review letters, Jan 30, 2011

We present a new method of determining the complete set of weak decay amplitudes (magnitude and p... more We present a new method of determining the complete set of weak decay amplitudes (magnitude and phase) involved in the process B → K * π. The method involves study of three body Dalitz plot and Bose symmetry of the pions, which arises from the subsequent decay of K * → Kπ.

Research paper thumbnail of An Interesting Observation of PDA Closure with Oral Paracetamol in Preterm Neonates

Journal of clinical neonatology, 2013

Patent ductus arteriosus (PDA), in which there is a persistent communication between the descendi... more Patent ductus arteriosus (PDA), in which there is a persistent communication between the descending thoracic aorta and the pulmonary artery that results from failure of normal physiologic closure of the fetal ductus, is one of the more common congenital heart defects in preterm neonates. The closure of PDA can be done with either Indomethacin or Brufen which are cyclooxygenase 1, 2 inhibitor; however these drugs are associated with side effects. We report an interesting findings of ductal closure in 10 preterm neonates (gestational age 27-33 wks) presenting with significant large PDA who had failed or had absolute contraindication with Brufen. These preterm neonates were treated with oral paracetamol in the dose of 15 mg/kg 8 hourly. The PDA closure was achieved within 48 h and there was no complication.

Research paper thumbnail of Prospective randomized comparison of retroperitoneoscopic vs open pyeloplasty with minimal incision: subjective and objective assessment in adults

Urology, 2014

To determine the subjective and objective outcomes of retroperitoneoscopic vs open pyeloplasty wi... more To determine the subjective and objective outcomes of retroperitoneoscopic vs open pyeloplasty with minimal incision in a prospective randomized comparison study.

Research paper thumbnail of Percutaneous Nephrolithotomy in Autosomal Dominant Polycystic Kidney Disease: Is it Different from Percutaneous Nephrolithotomy in Normal Kidney?

Current urology, 2013

Copyright © 2013 S. Karger AG, Basel Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com

Research paper thumbnail of Transperitoneal transvesical laparoscopic repair of vesicovaginal fistulae: experience of a tertiary care centre in northern India

Current urology, 2013

Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com Objective: To present our experience ... more Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com Objective: To present our experience of treating supratrigonal vesicovaginal fistulae by laparoscopic technique and their long-term follow-up. Material and Methods: Between January 2008 and June 2012, 28 cases of supratrigonal fistulas were repaired by laparoscopic transperitoneal transvesical technique with interposition flap. The obstetric fistula was present in 18 and gynecologic fistula in 10 patients. Single supratrigonal fistula was present in 26 patients and in 2 patients there were 2 fistulae lying side to side. The vaginal opening was closed as single layer interrupted suture and cystotomy closed as single layer continious suture by 3-0 polygalactin. The omentum was used as interposition flap in all except 2 cases in whom postero-superior vesical fold of peritoneum was used. The open conversion was required in 2 cases. The urethral catheter was removed in 4 weeks following a micturating cystogram. Result: The mean fistula size was 1.2 cm (range 0.8-2.5 cm). Open conversion was performed in 2 cases of whom one had excess carbon-dioxide retention and cardiac arrhythmia and in another case the needle of 3-0 polygalactin was avulsed and lost in peritoneal cavity which was recovered following laparotomy. All patients were continent following the catheter removal. The median follow-up is 24 months. None developed any complication related to laparoscopic repair till last follow-up.

Research paper thumbnail of Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF

Molecular and cellular biology, 2010

Alternative splicing and posttranslational modifications (PTMs) are major sources of protein dive... more Alternative splicing and posttranslational modifications (PTMs) are major sources of protein diversity in eukaryotic proteomes. The SR protein SF2/ASF is an oncoprotein that functions in pre-mRNA splicing, with additional roles in other posttranscriptional and translational events. Functional studies of SR protein PTMs have focused exclusively on the reversible phosphorylation of Ser residues in the C-terminal RS domain. We confirmed that human SF2/ASF is methylated at residues R93, R97, and R109, which were identified in a global proteomic analysis of Arg methylation, and further investigated whether these methylated residues regulate the properties of SF2/ASF. We show that the three arginines additively control the subcellular localization of SF2/ASF and that both the positive charge and the methylation state are important. Mutations that block methylation and remove the positive charge result in the cytoplasmic accumulation of SF2/ASF. The consequent decrease in nuclear SF2/ASF levels prevents it from modulating the alternative splicing of target genes, results in higher translation stimulation, and abrogates the enhancement of nonsense-mediated mRNA decay. This study addresses the mechanisms by which Arg methylation and the associated positive charge regulate the activities of SF2/ASF and emphasizes the significance of localization control for an oncoprotein with multiple functions in different cellular compartments.

Research paper thumbnail of A Sub-Band Based Technique for Low Power Medium Data Rate Ultra Wide Band Communication

A Sub-Band Based Technique for Low Power Medium Data Rate Ultra Wide Band Communication

2010 IEEE 71st Vehicular Technology Conference, 2010

Abstract A sub-band based ultra wideband (SUWB) system is proposed. The technique provides scope ... more Abstract A sub-band based ultra wideband (SUWB) system is proposed. The technique provides scope for using the ultra wideband bandwidth efficiently by exploiting the available link margin for short range communications. The bandwidth of 500 MHz or more is divided ...

Research paper thumbnail of Balloon distension of scrotum: a step to facilitate creation of dartos pouch during orchiopexy

Pediatric Surgery International, 2008

Dartos pouch creation is an important step of orchiopexy. We describe the use of the balloon of a... more Dartos pouch creation is an important step of orchiopexy. We describe the use of the balloon of a Foley catheter to distend the scrotal sac and stretch the scrotal parietal layers over it to facilitate bloodless and quick dissection for the creation of a dartos pouch.

Some concern may be raised that Foley catheter balloon might cause anaphylactic reaction in certain patients allergic to latex. We use Foley catheter balloon coated with silicone (Silastic) on a regular basis as they have a low immunogenic potential, and probably due to this reason, so far we have not encountered a single case of anaphylactic reaction caused by Foley catheter.

Research paper thumbnail of Etiological profile of stroke and its relation with the prothrombotic states

The Indian Journal of Pediatrics, 2009

Research paper thumbnail of An upper audio band based low data rate communication modem

An upper audio band based low data rate communication modem

2012 6th International Conference on Signal Processing and Communication Systems, 2012

ABSTRACT Readily available audio interfaces on mobile devices (speakers and microphones) can be u... more ABSTRACT Readily available audio interfaces on mobile devices (speakers and microphones) can be used to communicate data over a short range without the need for expensive infrastructure, using the imperceptible upper audio band (16-20KHz) of frequencies. A multi-tone Frequency Shift Keying (FSK) modulation is proposed for communication over the upper audio band. Error rate performance results for Multi-tone FSK modulation over white Gaussian noise channels are reported. Test results with a prototype modem developed are given. A sensing and spectrum access mechanism for devices with audio interfaces is proposed using protocol sequences.

Research paper thumbnail of Performance Evaluation of Variable-Rate Polynomial Phase Modulation

Performance Evaluation of Variable-Rate Polynomial Phase Modulation

MILCOM 2007 - IEEE Military Communications Conference, 2007

Page 1. 1-4244-1513-06/07/$25.00  2007 IEEE Page 1 of 7 PERFORMANCE EVALUATION OF VARIABLE-RATE ... more Page 1. 1-4244-1513-06/07/$25.00  2007 IEEE Page 1 of 7 PERFORMANCE EVALUATION OF VARIABLE-RATE POLYNOMIAL PHASE MODULATION Rahul Sinha, Guillermo E. Atkin, Chi Zhou Department of Electrical and ...

Research paper thumbnail of Digital phase modulation using polynomial phase functions

Digital phase modulation using polynomial phase functions

2007 IEEE International Conference on Electro/Information Technology, 2007

Page 1. IEEE EIT 2007 Proceedings 59 . 1-4244-0941-1/07/$25.00 cс2007 IEEE Digital Phase Modulati... more Page 1. IEEE EIT 2007 Proceedings 59 . 1-4244-0941-1/07/$25.00 cс2007 IEEE Digital Phase Modulation Using Polynomial Phase Functions Rahul Sinha, Student Member, IEEE, Guillermo. E. Atkin, Senior Member, IEEE, and Chi Zhou, Member, IEEE ...

Research paper thumbnail of Self-assessment

Self-assessment

Paediatrics and Child Health, 2013

Research paper thumbnail of Factors affecting response to medical management in patients of filarial chyluria: A prospective study

Factors affecting response to medical management in patients of filarial chyluria: A prospective study

Indian journal of urology : IJU : journal of the Urological Society of India, 2014

Filarial chyluria is a common problem in filarial endemic countries. Its management begins with m... more Filarial chyluria is a common problem in filarial endemic countries. Its management begins with medical therapy but some patients progress to require surgery. The present study aimed to determine factors affecting response to medical management in patients of filarial chyluria. This prospective study conducted between August 2008 and November 2012, included conservatively managed patients of chyluria. Demographic profile, clinical presentation, treatment history and urinary triglycerides (TGs) and cholesterol levels at baseline were compared between the responders and non-responders. Apart from the clinical grade of chyluria, hematuria was evaluated as an independent risk factor. Out of the 222 patients (mean age, 37.99 ± 13.29 years, 129 males), 31 patients failed to respond while 35 had a recurrence after initial response; the overall success rate being 70.3% at a mean follow-up of 25 months. No difference was observed in demographics, clinical presentation, presence of hematuria, disease duration and mean urinary TGs loss between responders and non-responders. On multivariate analysis, patients with treatment failure were found to have a higher-grade disease (14.3% Grade-I, 36.6% Grades-II and 60% Grade-III), higher number of pretreatment courses (1.59 ± 1.08 vs. 1.02 ± 0.79) and heavier cholesterol (26.54 ± 23.46 vs. 8.81 ± 8.55 mg/dl) loss at baseline compared with responders (P < 0.05). Conservative management has a success rate in excess of 70%, not affected by the disease chronicity, previous episodes and recurrent nature. However, higher-grade disease, extensive pre-treatment with drugs and higher urinary cholesterol loss at baseline are the predictors of poor response. Hematuria is not an independent poor risk factor for conservative management.

Research paper thumbnail of Production of biofuel and biochar by thermal pyrolysis of linseed seed

Production of biofuel and biochar by thermal pyrolysis of linseed seed

Biomass Conversion and Biorefinery, 2013

ABSTRACT The pyrolysis oil produced from nonfood related biomass can be used both as an energy so... more ABSTRACT The pyrolysis oil produced from nonfood related biomass can be used both as an energy source and a feedstock for chemical production. In the present work, linseed seeds (Linum usitatissimum L.) were pyrolyzed in a semibatch rector at a temperature range of 350–575 °C and at a heating rate of 20 °Cmin−1. The Fourier transform infrared analysis of the biofuel indicates the functional groups such as alkanes, alkenes, alkynes, and amines. The composition of the liquid product was analyzed using gas chromatography–mass spectrometry and found that the main constituents were oleanitrile, n-octadecane, 2-tridecanone, heptadecanenitrile, tetradecane, and 2-nonadecanone. The obtained liquid product can be used as a valuable chemical feedstock. The physical properties of the biofuel obtained were close to that of petroleum fractions. The pyrolysis char can be used as an adsorbent as well as a solid fuel due to its high calorific value.

Research paper thumbnail of Urodynamic and continence assessment of orthotropic neobladder reconstruction following radical cystectomy in bladder cancer; a prospective, blinded North Indian tertiary care experience

Urodynamic and continence assessment of orthotropic neobladder reconstruction following radical cystectomy in bladder cancer; a prospective, blinded North Indian tertiary care experience

South Asian journal of cancer, 2014

The aim of this study is to compare urodynamic and continence parameters among patients undergoin... more The aim of this study is to compare urodynamic and continence parameters among patients undergoing orthotropic neo-bladder substitution with sigmoid or ileal segments. Variations in the Urodynamic parameter between the continent and incontinent patients were also evaluated. From January 2008 to March 2012, 44 patients underwent ileal neobladder (IN) reconstruction and 36 patients underwent sigmoid neobladder (SN) reconstruction. Evaluation of Urodynamic and Continence parameters was performed at 12 months after surgery. The average capacity of IN and SN was 510 ml and 532 ml respectively. The voiding pressure, mean peak flow rates and post void residual urine (PVRU) for IN and SN were 27.5 cm H2O versus 37 cm H2O, 15ml/s versus 17ml/s and 36 ml versus 25 ml respectively. Daytime continence for IN and SN was 93% (41/44) and 89% (32/36), and night-time continence was 91% (40/44) and 78% (28/36) respectively. The compliance, maximum cystometric capacity and PVRU in the daytime continent (versus incontinent) were 61 (versus 41), 471 (versus 651) and 22 (versus 124) and in the night-time continent (versus incontinent) were 57 (versus 43), 437 (versus 654) and 18 (versus 105) respectively. A neobladder constructed from detubularized ileum or sigmoid achieves urodynamically proven adequate capacity and compliance with 89-93% daytime and 78-91% night time continence. Continent men when compared with incontinent (both daytime and night time) were more likely to have comparatively higher compliance, lower maximum cystometric capacity and lower PVRU. Urodynamic study could predict which incontinent men would improve with pelvic floor exercises and clean intermittent catheterization (CIC).

Research paper thumbnail of Tuning Cytokine Receptor Signaling by Re-orienting Dimer Geometry with Surrogate Ligands

Cell, Jan 12, 2015

Most cell-surface receptors for cytokines and growth factors signal as dimers, but it is unclear ... more Most cell-surface receptors for cytokines and growth factors signal as dimers, but it is unclear whether remodeling receptor dimer topology is a viable strategy to ''tune'' signaling output. We utilized diabodies (DA) as surrogate ligands in a prototypical dimeric receptor-ligand system, the cytokine Erythropoietin (EPO) and its receptor (EpoR), to dimerize EpoR ectodomains in non-native architectures. Diabodyinduced signaling amplitudes varied from full to minimal agonism, and structures of these DA/EpoR complexes differed in EpoR dimer orientation and proximity. Diabodies also elicited biased or differential activation of signaling pathways and gene expression profiles compared to EPO. Non-signaling diabodies inhibited proliferation of erythroid precursors from patients with a myeloproliferative neoplasm due to a constitutively active JAK2V617F mutation. Thus, intracellular oncogenic mutations causing ligand-independent receptor activation can be counteracted by extracellular ligands that reorient receptors into inactive dimer topologies. This approach has broad applications for tuning signaling output for many dimeric receptor systems.

Research paper thumbnail of Has new physics already been seen in B(d) meson decays?

Physical review letters, Jan 29, 2006

We show using a model independent approach that the deviation in measured B 0 d − B 0 d mixing ph... more We show using a model independent approach that the deviation in measured B 0 d − B 0 d mixing phase caused by pollution from another amplitude, within the Standard Model, is always less in magnitude, and has the same sign as the weak phase of the polluting amplitude. The exception is to have large destructive interference between the two amplitudes. We demonstrate that any deviation larger than a few degrees is only possible if the observed decay rate results from fine tuned cancellations between significantly larger quark level amplitudes. These simple observations have very significant consequences for signals of New Physics.

Research paper thumbnail of Urethral fistula following circumcision: salvaged by buccal mucosa graft urethroplasty

The Indian journal of surgery, 2009

Fistula following circumcision and at times accompanied by disfi gurement of the glans penis is a... more Fistula following circumcision and at times accompanied by disfi gurement of the glans penis is a common problem in our country, where a large number of circumcision is performed by untrained professionals. These complications may have profound negative psychological impact on the growing child. Herein, we report the successful closure of such fi stula using buccal mucosa, which occurred following circumcision (for phimosis) in a 15 year old boy. This resulted in the disfi gurement of the glans penis with a distal urethral fi stula. The fi stula was managed by dorsal onlay buccal mucosa graft urethroplasty (BMGU). The patient has excellent cosmesis at 6 months followup with a good urinary stream. This technique can give better results than those, where skin grafts or local fl aps are used for fi stula closure. BMGU is a versatile technique with good long term results. The cosmesis and functional results are satisfactory and help in improving the self esteem and sexual function in the years to come.

Research paper thumbnail of Subacute intestinal obstruction associated with a case of polycythemia vera--a rare combination

Subacute intestinal obstruction associated with a case of polycythemia vera--a rare combination

JPMA. The Journal of the Pakistan Medical Association, 2009

Primary Polycythemia Vera (PV) is difficult to diagnose due to its varied and atypical 1 presenta... more Primary Polycythemia Vera (PV) is difficult to diagnose due to its varied and atypical 1 presentation. It is a myeloproliferative disorder which is managed conservatively. We submit a case report of a patient who was admitted in surgery with subacute intestinal obstruction due to compression by a massive splenic cyst. The investigations showed PV causing splenomegaly.

Research paper thumbnail of Determination of weak amplitudes using Bose symmetry and Dalitz plots

Physical review letters, Jan 30, 2011

We present a new method of determining the complete set of weak decay amplitudes (magnitude and p... more We present a new method of determining the complete set of weak decay amplitudes (magnitude and phase) involved in the process B → K * π. The method involves study of three body Dalitz plot and Bose symmetry of the pions, which arises from the subsequent decay of K * → Kπ.

Research paper thumbnail of An Interesting Observation of PDA Closure with Oral Paracetamol in Preterm Neonates

Journal of clinical neonatology, 2013

Patent ductus arteriosus (PDA), in which there is a persistent communication between the descendi... more Patent ductus arteriosus (PDA), in which there is a persistent communication between the descending thoracic aorta and the pulmonary artery that results from failure of normal physiologic closure of the fetal ductus, is one of the more common congenital heart defects in preterm neonates. The closure of PDA can be done with either Indomethacin or Brufen which are cyclooxygenase 1, 2 inhibitor; however these drugs are associated with side effects. We report an interesting findings of ductal closure in 10 preterm neonates (gestational age 27-33 wks) presenting with significant large PDA who had failed or had absolute contraindication with Brufen. These preterm neonates were treated with oral paracetamol in the dose of 15 mg/kg 8 hourly. The PDA closure was achieved within 48 h and there was no complication.

Research paper thumbnail of Prospective randomized comparison of retroperitoneoscopic vs open pyeloplasty with minimal incision: subjective and objective assessment in adults

Urology, 2014

To determine the subjective and objective outcomes of retroperitoneoscopic vs open pyeloplasty wi... more To determine the subjective and objective outcomes of retroperitoneoscopic vs open pyeloplasty with minimal incision in a prospective randomized comparison study.

Research paper thumbnail of Percutaneous Nephrolithotomy in Autosomal Dominant Polycystic Kidney Disease: Is it Different from Percutaneous Nephrolithotomy in Normal Kidney?

Current urology, 2013

Copyright © 2013 S. Karger AG, Basel Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com

Research paper thumbnail of Transperitoneal transvesical laparoscopic repair of vesicovaginal fistulae: experience of a tertiary care centre in northern India

Current urology, 2013

Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com Objective: To present our experience ... more Fax +41 61 306 12 34 E-Mail karger@karger.ch www.karger.com Objective: To present our experience of treating supratrigonal vesicovaginal fistulae by laparoscopic technique and their long-term follow-up. Material and Methods: Between January 2008 and June 2012, 28 cases of supratrigonal fistulas were repaired by laparoscopic transperitoneal transvesical technique with interposition flap. The obstetric fistula was present in 18 and gynecologic fistula in 10 patients. Single supratrigonal fistula was present in 26 patients and in 2 patients there were 2 fistulae lying side to side. The vaginal opening was closed as single layer interrupted suture and cystotomy closed as single layer continious suture by 3-0 polygalactin. The omentum was used as interposition flap in all except 2 cases in whom postero-superior vesical fold of peritoneum was used. The open conversion was required in 2 cases. The urethral catheter was removed in 4 weeks following a micturating cystogram. Result: The mean fistula size was 1.2 cm (range 0.8-2.5 cm). Open conversion was performed in 2 cases of whom one had excess carbon-dioxide retention and cardiac arrhythmia and in another case the needle of 3-0 polygalactin was avulsed and lost in peritoneal cavity which was recovered following laparotomy. All patients were continent following the catheter removal. The median follow-up is 24 months. None developed any complication related to laparoscopic repair till last follow-up.

Research paper thumbnail of Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF

Molecular and cellular biology, 2010

Alternative splicing and posttranslational modifications (PTMs) are major sources of protein dive... more Alternative splicing and posttranslational modifications (PTMs) are major sources of protein diversity in eukaryotic proteomes. The SR protein SF2/ASF is an oncoprotein that functions in pre-mRNA splicing, with additional roles in other posttranscriptional and translational events. Functional studies of SR protein PTMs have focused exclusively on the reversible phosphorylation of Ser residues in the C-terminal RS domain. We confirmed that human SF2/ASF is methylated at residues R93, R97, and R109, which were identified in a global proteomic analysis of Arg methylation, and further investigated whether these methylated residues regulate the properties of SF2/ASF. We show that the three arginines additively control the subcellular localization of SF2/ASF and that both the positive charge and the methylation state are important. Mutations that block methylation and remove the positive charge result in the cytoplasmic accumulation of SF2/ASF. The consequent decrease in nuclear SF2/ASF levels prevents it from modulating the alternative splicing of target genes, results in higher translation stimulation, and abrogates the enhancement of nonsense-mediated mRNA decay. This study addresses the mechanisms by which Arg methylation and the associated positive charge regulate the activities of SF2/ASF and emphasizes the significance of localization control for an oncoprotein with multiple functions in different cellular compartments.

Research paper thumbnail of A Sub-Band Based Technique for Low Power Medium Data Rate Ultra Wide Band Communication

A Sub-Band Based Technique for Low Power Medium Data Rate Ultra Wide Band Communication

2010 IEEE 71st Vehicular Technology Conference, 2010

Abstract A sub-band based ultra wideband (SUWB) system is proposed. The technique provides scope ... more Abstract A sub-band based ultra wideband (SUWB) system is proposed. The technique provides scope for using the ultra wideband bandwidth efficiently by exploiting the available link margin for short range communications. The bandwidth of 500 MHz or more is divided ...

Research paper thumbnail of Balloon distension of scrotum: a step to facilitate creation of dartos pouch during orchiopexy

Pediatric Surgery International, 2008

Dartos pouch creation is an important step of orchiopexy. We describe the use of the balloon of a... more Dartos pouch creation is an important step of orchiopexy. We describe the use of the balloon of a Foley catheter to distend the scrotal sac and stretch the scrotal parietal layers over it to facilitate bloodless and quick dissection for the creation of a dartos pouch.

Some concern may be raised that Foley catheter balloon might cause anaphylactic reaction in certain patients allergic to latex. We use Foley catheter balloon coated with silicone (Silastic) on a regular basis as they have a low immunogenic potential, and probably due to this reason, so far we have not encountered a single case of anaphylactic reaction caused by Foley catheter.

Research paper thumbnail of Etiological profile of stroke and its relation with the prothrombotic states

The Indian Journal of Pediatrics, 2009

Research paper thumbnail of An upper audio band based low data rate communication modem

An upper audio band based low data rate communication modem

2012 6th International Conference on Signal Processing and Communication Systems, 2012

ABSTRACT Readily available audio interfaces on mobile devices (speakers and microphones) can be u... more ABSTRACT Readily available audio interfaces on mobile devices (speakers and microphones) can be used to communicate data over a short range without the need for expensive infrastructure, using the imperceptible upper audio band (16-20KHz) of frequencies. A multi-tone Frequency Shift Keying (FSK) modulation is proposed for communication over the upper audio band. Error rate performance results for Multi-tone FSK modulation over white Gaussian noise channels are reported. Test results with a prototype modem developed are given. A sensing and spectrum access mechanism for devices with audio interfaces is proposed using protocol sequences.

Research paper thumbnail of Performance Evaluation of Variable-Rate Polynomial Phase Modulation

Performance Evaluation of Variable-Rate Polynomial Phase Modulation

MILCOM 2007 - IEEE Military Communications Conference, 2007

Page 1. 1-4244-1513-06/07/$25.00  2007 IEEE Page 1 of 7 PERFORMANCE EVALUATION OF VARIABLE-RATE ... more Page 1. 1-4244-1513-06/07/$25.00  2007 IEEE Page 1 of 7 PERFORMANCE EVALUATION OF VARIABLE-RATE POLYNOMIAL PHASE MODULATION Rahul Sinha, Guillermo E. Atkin, Chi Zhou Department of Electrical and ...

Research paper thumbnail of Digital phase modulation using polynomial phase functions

Digital phase modulation using polynomial phase functions

2007 IEEE International Conference on Electro/Information Technology, 2007

Page 1. IEEE EIT 2007 Proceedings 59 . 1-4244-0941-1/07/$25.00 cс2007 IEEE Digital Phase Modulati... more Page 1. IEEE EIT 2007 Proceedings 59 . 1-4244-0941-1/07/$25.00 cс2007 IEEE Digital Phase Modulation Using Polynomial Phase Functions Rahul Sinha, Student Member, IEEE, Guillermo. E. Atkin, Senior Member, IEEE, and Chi Zhou, Member, IEEE ...

Research paper thumbnail of Self-assessment

Self-assessment

Paediatrics and Child Health, 2013

Research paper thumbnail of Factors affecting response to medical management in patients of filarial chyluria: A prospective study

Factors affecting response to medical management in patients of filarial chyluria: A prospective study

Indian journal of urology : IJU : journal of the Urological Society of India, 2014

Filarial chyluria is a common problem in filarial endemic countries. Its management begins with m... more Filarial chyluria is a common problem in filarial endemic countries. Its management begins with medical therapy but some patients progress to require surgery. The present study aimed to determine factors affecting response to medical management in patients of filarial chyluria. This prospective study conducted between August 2008 and November 2012, included conservatively managed patients of chyluria. Demographic profile, clinical presentation, treatment history and urinary triglycerides (TGs) and cholesterol levels at baseline were compared between the responders and non-responders. Apart from the clinical grade of chyluria, hematuria was evaluated as an independent risk factor. Out of the 222 patients (mean age, 37.99 ± 13.29 years, 129 males), 31 patients failed to respond while 35 had a recurrence after initial response; the overall success rate being 70.3% at a mean follow-up of 25 months. No difference was observed in demographics, clinical presentation, presence of hematuria, disease duration and mean urinary TGs loss between responders and non-responders. On multivariate analysis, patients with treatment failure were found to have a higher-grade disease (14.3% Grade-I, 36.6% Grades-II and 60% Grade-III), higher number of pretreatment courses (1.59 ± 1.08 vs. 1.02 ± 0.79) and heavier cholesterol (26.54 ± 23.46 vs. 8.81 ± 8.55 mg/dl) loss at baseline compared with responders (P < 0.05). Conservative management has a success rate in excess of 70%, not affected by the disease chronicity, previous episodes and recurrent nature. However, higher-grade disease, extensive pre-treatment with drugs and higher urinary cholesterol loss at baseline are the predictors of poor response. Hematuria is not an independent poor risk factor for conservative management.

Research paper thumbnail of Production of biofuel and biochar by thermal pyrolysis of linseed seed

Production of biofuel and biochar by thermal pyrolysis of linseed seed

Biomass Conversion and Biorefinery, 2013

ABSTRACT The pyrolysis oil produced from nonfood related biomass can be used both as an energy so... more ABSTRACT The pyrolysis oil produced from nonfood related biomass can be used both as an energy source and a feedstock for chemical production. In the present work, linseed seeds (Linum usitatissimum L.) were pyrolyzed in a semibatch rector at a temperature range of 350–575 °C and at a heating rate of 20 °Cmin−1. The Fourier transform infrared analysis of the biofuel indicates the functional groups such as alkanes, alkenes, alkynes, and amines. The composition of the liquid product was analyzed using gas chromatography–mass spectrometry and found that the main constituents were oleanitrile, n-octadecane, 2-tridecanone, heptadecanenitrile, tetradecane, and 2-nonadecanone. The obtained liquid product can be used as a valuable chemical feedstock. The physical properties of the biofuel obtained were close to that of petroleum fractions. The pyrolysis char can be used as an adsorbent as well as a solid fuel due to its high calorific value.

Research paper thumbnail of Urodynamic and continence assessment of orthotropic neobladder reconstruction following radical cystectomy in bladder cancer; a prospective, blinded North Indian tertiary care experience

Urodynamic and continence assessment of orthotropic neobladder reconstruction following radical cystectomy in bladder cancer; a prospective, blinded North Indian tertiary care experience

South Asian journal of cancer, 2014

The aim of this study is to compare urodynamic and continence parameters among patients undergoin... more The aim of this study is to compare urodynamic and continence parameters among patients undergoing orthotropic neo-bladder substitution with sigmoid or ileal segments. Variations in the Urodynamic parameter between the continent and incontinent patients were also evaluated. From January 2008 to March 2012, 44 patients underwent ileal neobladder (IN) reconstruction and 36 patients underwent sigmoid neobladder (SN) reconstruction. Evaluation of Urodynamic and Continence parameters was performed at 12 months after surgery. The average capacity of IN and SN was 510 ml and 532 ml respectively. The voiding pressure, mean peak flow rates and post void residual urine (PVRU) for IN and SN were 27.5 cm H2O versus 37 cm H2O, 15ml/s versus 17ml/s and 36 ml versus 25 ml respectively. Daytime continence for IN and SN was 93% (41/44) and 89% (32/36), and night-time continence was 91% (40/44) and 78% (28/36) respectively. The compliance, maximum cystometric capacity and PVRU in the daytime continent (versus incontinent) were 61 (versus 41), 471 (versus 651) and 22 (versus 124) and in the night-time continent (versus incontinent) were 57 (versus 43), 437 (versus 654) and 18 (versus 105) respectively. A neobladder constructed from detubularized ileum or sigmoid achieves urodynamically proven adequate capacity and compliance with 89-93% daytime and 78-91% night time continence. Continent men when compared with incontinent (both daytime and night time) were more likely to have comparatively higher compliance, lower maximum cystometric capacity and lower PVRU. Urodynamic study could predict which incontinent men would improve with pelvic floor exercises and clean intermittent catheterization (CIC).