Raimundo Wilson Carvalho - Academia.edu (original) (raw)
Papers by Raimundo Wilson Carvalho
Parasitol Dia, Jun 1, 1996
Microscopy Research and Technique, 2009
The salivary glands of Anocentor nitens (Neumann,1897) occur in pairs and are located in the ante... more The salivary glands of Anocentor nitens (Neumann,1897) occur in pairs and are located in the anterolateral region of the general cavity, with milky white color and approximately equal sizes. They consist of a secretory portion and an excretion duct. In some glandular acini, all the cells had a basophilic appearance they were stained by hematoxylin, whereas others presented cells with different staining affinities. In this work, we describe the variations observed in these glands during the feeding cycle of ticks [after feeding (0 h) and successively at 24, 48, 72, 96, 120, and 144 h]. The cells stained by hematoxylin were shown to be more reactive to Alcian blue, thus demonstrating the presence of acid glycosaminoglycans, whereas those stained using eosin presented weak or no reaction. A strong reaction was found by the use of the periodic acid-Schiff (PAS) technique, thereby suggesting the presence of glycogen and/or glycoconjugates containing hexose, confirmed by using salivary amylase before PAS, with partial destaining of the slides. Continuing presence of residual staining in these cells suggests the presence of glycoconjugates containing hexose. Cells with nuclei of circular outline and few granules (of different sizes) were found in type II acini, 72 h after collection. Type I acini presented wide lumina and walls composed of larger numbers of cells of cubic to cylindrical shape. The pronounced degranulation shown in this study over the course of the feeding cycle was associated with the release of substances for oviposition.
Revista Brasileira De Hematologia E Hemoterapia, 2007
Revista da Sociedade Brasileira de Medicina Tropical, 2015
Aedes aegypti eggs can be collected from the water surface. Aedes aegypti oviposition from 97 fie... more Aedes aegypti eggs can be collected from the water surface. Aedes aegypti oviposition from 97 field ovitraps was studied. Of the 16,016 eggs collected, 11,439 were obtained from paddles in ovitraps and 4,577 from water. Further, 89 (91.8%) traps contained eggs on water and 22 (22.7%) traps contained eggs only on water. In field traps, Aedes aegypti females usually oviposit some eggs on water surface suggesting that they might also oviposit on water of some natural breeding, and this possibility needs to be investigated. Eggs oviposited on water need to be considered for collecting trap data.
International Journal of Biology, 2015
During studies on tabanid fauna on Marambaia Island, Mangaratiba, Brazil, the authors captured 71... more During studies on tabanid fauna on Marambaia Island, Mangaratiba, Brazil, the authors captured 71specimens of two species of solitaire sand wasps hunting horseflies, Stictia punctata (Fabricius, 1775) and Stictia signata signata (Linnaeus, 1758). Wasps interact with horseflies demonstrating outstanding behavior, interrupting the hematophagism, which enhances tabanids ability to transmit pathogenic agents to natural hosts.
Revista da Sociedade Brasileira de Medicina Tropical, 2011
The outbreak occurred between February and June 2006 and included identification of the cases, an... more The outbreak occurred between February and June 2006 and included identification of the cases, analysis of medical records, cultures from environmental sources, resistance analyses and genotyping profile of Serratia marcescens. The cultures were composed of 13 blood isolates, 17 rectal and hand swabs and air sampling. The data obtained by pulsed-field gel electrophoresis exhibited three strains that contaminated 24 patients. Systemic infection was the most common in neonates with lower weight, long periods of hospitalization, premature delivery and the use of mechanical ventilation. This investigation revealed the multifactorial nature of the outbreak. An endemic clone of S. marcescens was detected.
Journal of Neuro-Oncology, 2012
Medulloblastoma is a highly cellular malignant embryonal neoplasm, being the most common malignan... more Medulloblastoma is a highly cellular malignant embryonal neoplasm, being the most common malignant pediatric brain tumor, accounting for 20-25 % of pediatric central nervous system tumors. To investigate the effect of the TP53 Arg72Pro single-nucleotide polymorphism (SNP) on clinicopathological and phenotypic parameters, we performed a case-controlled study of 122 patients and 122 healthy controls from Brazil. No significant associations were found between the TP53 Arg72Pro genotypes and the clinicopathological parameters studied. Compared with Arg/Arg, which is the most common genotype in the study population, both the Arg/Pro and Pro/Pro genotypes did not influence the medulloblastoma development risk [odds ratio (OR) = 1.36 and P = 0.339 for the Arg/Pro genotype; OR = 1.50 and P = 0.389 for the Pro/Pro genotype]. With regard to prognosis, disease-free survival was not significantly different among the TP53 Arg72Pro SNP genotypes (P [ 0.05), but the less frequent genotype (Pro/ Pro) was associated with shorter overall survival of medulloblastoma patients (P = 0.021). These data suggest that, although there is no association between the TP53 Arg72Pro SNP and medulloblastoma risk, the Pro/Pro genotype is associated with shorter overall survival of patients submitted to adjuvant therapy. Nevertheless, due to the interethnic composition of the Brazilian population, future studies on larger populations from other parts of the world are essential for a definitive conclusion on the function of the TP53 Arg72Pro SNP.
Fisioter Bras, Jun 1, 2005
Colombia Medica, Jun 1, 2009
Revista Colombiana De Entomologia, Jun 1, 2010
Revista Cubana De Medicina Tropical, Feb 5, 2014
Revista Cubana De Medicina Tropical, Mar 1, 2014
Revista Uniabeu, Aug 8, 2012
No Parque Estadual da Pedra Branca, Rio de Janeiro, durante 24 meses, foram feitas captura, inspe... more No Parque Estadual da Pedra Branca, Rio de Janeiro, durante 24 meses, foram feitas captura, inspeção, liberação, e foi avaliada a distribuição dos 64 roedores e 96 marsupiais capturados entre 150 e 600m de altitude, relacionando com fatores ambientes. Foi avaliada a regeneração da cobertura vegetal, e do entorno, considerada constituída basicamente por vegetação secundária. Entre os marsupiais a dominância foi de Didelphis aurita, e entre os roedores foi de Guerlinguetus ingrami. A correlação entre o número de roedores capturados e as cotas altitudinais, apesar de positiva, foi não significativa, e, para os marsupiais, foi negativa e também não significativa.
Entomología y Vectores
Fleas of small rodents from the South Region of Rio de Janeiro State, Brazil -The authors present... more Fleas of small rodents from the South Region of Rio de Janeiro State, Brazil -The authors present the small rodent flea fauna of two municipalities of South region of the Rio de Janeiro State, relating the mean intensity on rodents and flea prevalency. Specimens were caught from March 1996 to April 1997 and from September to October 1997 in Ilha Grande, Angra dos Reis district and Saco do Mamanguá, Paraty district. Altogether seven species of small rodents and eight fleas species were obtained. The following represents new host records, Hechtiella lakoi (Guimarães, 1948) from Oligoryzomys nigripes (Wagner, 1945) and Polygenis (Neopolygenis) pradoi (Wagner, 1937) from Rhypidomys mastacalis (Lund, 1841) in South Region
Parasitol Dia, Jun 1, 1996
Microscopy Research and Technique, 2009
The salivary glands of Anocentor nitens (Neumann,1897) occur in pairs and are located in the ante... more The salivary glands of Anocentor nitens (Neumann,1897) occur in pairs and are located in the anterolateral region of the general cavity, with milky white color and approximately equal sizes. They consist of a secretory portion and an excretion duct. In some glandular acini, all the cells had a basophilic appearance they were stained by hematoxylin, whereas others presented cells with different staining affinities. In this work, we describe the variations observed in these glands during the feeding cycle of ticks [after feeding (0 h) and successively at 24, 48, 72, 96, 120, and 144 h]. The cells stained by hematoxylin were shown to be more reactive to Alcian blue, thus demonstrating the presence of acid glycosaminoglycans, whereas those stained using eosin presented weak or no reaction. A strong reaction was found by the use of the periodic acid-Schiff (PAS) technique, thereby suggesting the presence of glycogen and/or glycoconjugates containing hexose, confirmed by using salivary amylase before PAS, with partial destaining of the slides. Continuing presence of residual staining in these cells suggests the presence of glycoconjugates containing hexose. Cells with nuclei of circular outline and few granules (of different sizes) were found in type II acini, 72 h after collection. Type I acini presented wide lumina and walls composed of larger numbers of cells of cubic to cylindrical shape. The pronounced degranulation shown in this study over the course of the feeding cycle was associated with the release of substances for oviposition.
Revista Brasileira De Hematologia E Hemoterapia, 2007
Revista da Sociedade Brasileira de Medicina Tropical, 2015
Aedes aegypti eggs can be collected from the water surface. Aedes aegypti oviposition from 97 fie... more Aedes aegypti eggs can be collected from the water surface. Aedes aegypti oviposition from 97 field ovitraps was studied. Of the 16,016 eggs collected, 11,439 were obtained from paddles in ovitraps and 4,577 from water. Further, 89 (91.8%) traps contained eggs on water and 22 (22.7%) traps contained eggs only on water. In field traps, Aedes aegypti females usually oviposit some eggs on water surface suggesting that they might also oviposit on water of some natural breeding, and this possibility needs to be investigated. Eggs oviposited on water need to be considered for collecting trap data.
International Journal of Biology, 2015
During studies on tabanid fauna on Marambaia Island, Mangaratiba, Brazil, the authors captured 71... more During studies on tabanid fauna on Marambaia Island, Mangaratiba, Brazil, the authors captured 71specimens of two species of solitaire sand wasps hunting horseflies, Stictia punctata (Fabricius, 1775) and Stictia signata signata (Linnaeus, 1758). Wasps interact with horseflies demonstrating outstanding behavior, interrupting the hematophagism, which enhances tabanids ability to transmit pathogenic agents to natural hosts.
Revista da Sociedade Brasileira de Medicina Tropical, 2011
The outbreak occurred between February and June 2006 and included identification of the cases, an... more The outbreak occurred between February and June 2006 and included identification of the cases, analysis of medical records, cultures from environmental sources, resistance analyses and genotyping profile of Serratia marcescens. The cultures were composed of 13 blood isolates, 17 rectal and hand swabs and air sampling. The data obtained by pulsed-field gel electrophoresis exhibited three strains that contaminated 24 patients. Systemic infection was the most common in neonates with lower weight, long periods of hospitalization, premature delivery and the use of mechanical ventilation. This investigation revealed the multifactorial nature of the outbreak. An endemic clone of S. marcescens was detected.
Journal of Neuro-Oncology, 2012
Medulloblastoma is a highly cellular malignant embryonal neoplasm, being the most common malignan... more Medulloblastoma is a highly cellular malignant embryonal neoplasm, being the most common malignant pediatric brain tumor, accounting for 20-25 % of pediatric central nervous system tumors. To investigate the effect of the TP53 Arg72Pro single-nucleotide polymorphism (SNP) on clinicopathological and phenotypic parameters, we performed a case-controlled study of 122 patients and 122 healthy controls from Brazil. No significant associations were found between the TP53 Arg72Pro genotypes and the clinicopathological parameters studied. Compared with Arg/Arg, which is the most common genotype in the study population, both the Arg/Pro and Pro/Pro genotypes did not influence the medulloblastoma development risk [odds ratio (OR) = 1.36 and P = 0.339 for the Arg/Pro genotype; OR = 1.50 and P = 0.389 for the Pro/Pro genotype]. With regard to prognosis, disease-free survival was not significantly different among the TP53 Arg72Pro SNP genotypes (P [ 0.05), but the less frequent genotype (Pro/ Pro) was associated with shorter overall survival of medulloblastoma patients (P = 0.021). These data suggest that, although there is no association between the TP53 Arg72Pro SNP and medulloblastoma risk, the Pro/Pro genotype is associated with shorter overall survival of patients submitted to adjuvant therapy. Nevertheless, due to the interethnic composition of the Brazilian population, future studies on larger populations from other parts of the world are essential for a definitive conclusion on the function of the TP53 Arg72Pro SNP.
Fisioter Bras, Jun 1, 2005
Colombia Medica, Jun 1, 2009
Revista Colombiana De Entomologia, Jun 1, 2010
Revista Cubana De Medicina Tropical, Feb 5, 2014
Revista Cubana De Medicina Tropical, Mar 1, 2014
Revista Uniabeu, Aug 8, 2012
No Parque Estadual da Pedra Branca, Rio de Janeiro, durante 24 meses, foram feitas captura, inspe... more No Parque Estadual da Pedra Branca, Rio de Janeiro, durante 24 meses, foram feitas captura, inspeção, liberação, e foi avaliada a distribuição dos 64 roedores e 96 marsupiais capturados entre 150 e 600m de altitude, relacionando com fatores ambientes. Foi avaliada a regeneração da cobertura vegetal, e do entorno, considerada constituída basicamente por vegetação secundária. Entre os marsupiais a dominância foi de Didelphis aurita, e entre os roedores foi de Guerlinguetus ingrami. A correlação entre o número de roedores capturados e as cotas altitudinais, apesar de positiva, foi não significativa, e, para os marsupiais, foi negativa e também não significativa.
Entomología y Vectores
Fleas of small rodents from the South Region of Rio de Janeiro State, Brazil -The authors present... more Fleas of small rodents from the South Region of Rio de Janeiro State, Brazil -The authors present the small rodent flea fauna of two municipalities of South region of the Rio de Janeiro State, relating the mean intensity on rodents and flea prevalency. Specimens were caught from March 1996 to April 1997 and from September to October 1997 in Ilha Grande, Angra dos Reis district and Saco do Mamanguá, Paraty district. Altogether seven species of small rodents and eight fleas species were obtained. The following represents new host records, Hechtiella lakoi (Guimarães, 1948) from Oligoryzomys nigripes (Wagner, 1945) and Polygenis (Neopolygenis) pradoi (Wagner, 1937) from Rhypidomys mastacalis (Lund, 1841) in South Region