Rama Ramani - Academia.edu (original) (raw)

Papers by Rama Ramani

Journal of Clinical Microbiology, Dec 1, 2009

This article cites 18 articles, 12 of which can be accessed free

This article cites 11 articles, 3 of which can be accessed free at:

F1000 - Post-publication peer review of the biomedical literature, 2010

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2011

Journal of postgraduate medicine

During 1st week of post-operative period, a 28 year old female patient operated for left cerebell... more During 1st week of post-operative period, a 28 year old female patient operated for left cerebellopontine angle tumor, continued to get fever. Lumbar puncture did not reveal any organisms. She responded to ciprofloxacin. Two months later, she was readmitted with signs and symptoms of meningitis. The CSF tapped on lumbar puncture grew Xanthomonas maltophilia, Gram negative bacilli, sensitive to various antibiotics, ciprofloxacin being one of them. The patient was given ciprofloxacin for 3 weeks. On follow up, a year later she was found to be asymptomatic.

Indian journal of pathology & microbiology, 1994

Sixty bronchoalveolar lavage samples collected from lung cancer patients attending the Pulmonary ... more Sixty bronchoalveolar lavage samples collected from lung cancer patients attending the Pulmonary Tuberculosis and Chest Diseases Unit of the Kasturba Medical College Hospital, Manipal; were cultured for both the aerobic and anaerobic organisms. Fifty nine samples yielded bacteria in pure culture. Pseudomonas aeruginosa (34.8%) was the commonest aerobe, Peptostreptococcus was the commonest anaerobe (45.2%) isolated. Bacteroides fragilis was isolated in (23.8%) of cases. Gentamycin was found to be effective against aerobes, Metronidazole and Rifampicin against anaerobes.

Mycopathologia, 2007

Coccidioidomycosis is a systemic fungal infection endemic in Southwestern United States, Mexico, ... more Coccidioidomycosis is a systemic fungal infection endemic in Southwestern United States, Mexico, Central and South America. The causal agents are Coccidioides immitis and C. posadasii. A large number of cases of coccidioidomycosis in New York State residents were identified. We compared susceptibility profiles of these isolates and of C. immitis isolates from California using mycelial phase inoculum and CLSI (NCCLS) M38-A broth microdilution protocol. Minimum fungicidal concentrations (MFC) were also determined. Results indicated that geometric mean MICs of amphotericin B (AMB, 0.06 microg/ml), fluconazole (FLC, 8.0 microg/ml), itraconazole (ITC, 0.07 microg/ml), ketoconazole (KTC, 0.04 microg/ml), voriconazole (VRC, 0.04 microg/ml), posaconazole (PSC, 0.17 microg/ml) and caspofungin (CSP, 0.15 microg/ml) were in susceptible range as per breakpoints published for pathogenic Candida species. However, geometric MFC for FLC was relatively higher (52.4 microg/ml). Also, no significant difference in MIC and MFC values was evident for C. immitis and C. posadasii isolates. In conclusion, current methods for antifungal susceptibility testing yield reproducible profiles for Coccidioides species, which appear to be highly susceptible to most antifungal agents.

Journal of Antimicrobial Chemotherapy, 2005

MMWR. Morbidity and Mortality Weekly Report

F1000 - Post-publication peer review of the biomedical literature, 2011

, 102 conjunctival swab specimens were collected for surveillance purposes from patients across J... more , 102 conjunctival swab specimens were collected for surveillance purposes from patients across Japan suspected of having epidemic keratoconjunctivitis (EKC). Human adenovirus (HAdV) DNA was detected in 61 samples by PCR, though the HAdV type for 6 of the PCR-positive samples could not be determined by phylogenetic analysis using a partial hexon gene sequence. Moreover, for 2 months from January 2009, HAdV strains with identical sequences were isolated from five conjunctival swab samples obtained from EKC patients in five different regions of Japan. For the analyses of the 11 samples mentioned above, we determined the nucleotide sequences of the entire penton base, hexon, and fiber genes and early 3 (E3) region, which are variable regions among HAdV types, and compared them to those of other HAdV species D strains. The nucleotide sequences of loops 1 and 2 in the hexons of all 11 samples showed high degrees of identity with those of the HAdV type 15 (HAdV-15) and HAdV-29 prototype strains. However, the fiber gene and E3 region sequences showed high degrees of identity with those of HAdV-9, and the penton base gene sequence showed a high degree of identity with the penton base gene sequences of HAdV-9 and-26. Moreover, the complete genome sequence of the 2307-S strain, which was isolated by viral culture from 1 of the 11 samples, was determined. The 2307-S strain was a recombinant HAdV between HAdV-9,-15,-26,-29, and/or another HAdV type; however, the recombination sites in the genome were not obvious. We propose that this virus is a novel intertypic recombinant, HAdV-15/29/H9, and may be an etiological agent of EKC.

F1000 - Post-publication peer review of the biomedical literature, 2010

Herpes simplex virus-1 (HSV-1) establishes lifelong latency in peripheral neurons where productiv... more Herpes simplex virus-1 (HSV-1) establishes lifelong latency in peripheral neurons where productive replication is suppressed. While periodic reactivation results in virus production, the molecular basis of neuronal latency remains incompletely understood. Using a primary neuronal culture system of HSV-1 latency and reactivation, we show that continuous signaling through the phosphatidylinositol 3-kinase (PI3-K) pathway triggered by nerve growth factor (NGF)-binding to the TrkA receptor tyrosine kinase (RTK) is instrumental in maintaining latent HSV-1. The PI3-K p110α catalytic subunit, but not the β or δ isoforms, is specifically required to activate 3phosphoinositide-dependent protein kinase-1 (PDK1) and sustain latency. Disrupting this pathway leads to virus reactivation. EGF and GDNF, two other growth factors capable of activating PI3-K and PDK1 but that differ from NGF in their ability to persistently activate Akt, do not fully support HSV-1 latency. Thus the nature of RTK-signaling is a critical host parameter that regulates the HSV-1 latent-lytic switch.

F1000 - Post-publication peer review of the biomedical literature, 2011

Journal of Clinical Microbiology, Dec 1, 2009

This article cites 18 articles, 12 of which can be accessed free

This article cites 11 articles, 3 of which can be accessed free at:

F1000 - Post-publication peer review of the biomedical literature, 2010

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2012

F1000 - Post-publication peer review of the biomedical literature, 2011

Journal of postgraduate medicine

During 1st week of post-operative period, a 28 year old female patient operated for left cerebell... more During 1st week of post-operative period, a 28 year old female patient operated for left cerebellopontine angle tumor, continued to get fever. Lumbar puncture did not reveal any organisms. She responded to ciprofloxacin. Two months later, she was readmitted with signs and symptoms of meningitis. The CSF tapped on lumbar puncture grew Xanthomonas maltophilia, Gram negative bacilli, sensitive to various antibiotics, ciprofloxacin being one of them. The patient was given ciprofloxacin for 3 weeks. On follow up, a year later she was found to be asymptomatic.

Indian journal of pathology & microbiology, 1994

Sixty bronchoalveolar lavage samples collected from lung cancer patients attending the Pulmonary ... more Sixty bronchoalveolar lavage samples collected from lung cancer patients attending the Pulmonary Tuberculosis and Chest Diseases Unit of the Kasturba Medical College Hospital, Manipal; were cultured for both the aerobic and anaerobic organisms. Fifty nine samples yielded bacteria in pure culture. Pseudomonas aeruginosa (34.8%) was the commonest aerobe, Peptostreptococcus was the commonest anaerobe (45.2%) isolated. Bacteroides fragilis was isolated in (23.8%) of cases. Gentamycin was found to be effective against aerobes, Metronidazole and Rifampicin against anaerobes.

Mycopathologia, 2007

Coccidioidomycosis is a systemic fungal infection endemic in Southwestern United States, Mexico, ... more Coccidioidomycosis is a systemic fungal infection endemic in Southwestern United States, Mexico, Central and South America. The causal agents are Coccidioides immitis and C. posadasii. A large number of cases of coccidioidomycosis in New York State residents were identified. We compared susceptibility profiles of these isolates and of C. immitis isolates from California using mycelial phase inoculum and CLSI (NCCLS) M38-A broth microdilution protocol. Minimum fungicidal concentrations (MFC) were also determined. Results indicated that geometric mean MICs of amphotericin B (AMB, 0.06 microg/ml), fluconazole (FLC, 8.0 microg/ml), itraconazole (ITC, 0.07 microg/ml), ketoconazole (KTC, 0.04 microg/ml), voriconazole (VRC, 0.04 microg/ml), posaconazole (PSC, 0.17 microg/ml) and caspofungin (CSP, 0.15 microg/ml) were in susceptible range as per breakpoints published for pathogenic Candida species. However, geometric MFC for FLC was relatively higher (52.4 microg/ml). Also, no significant difference in MIC and MFC values was evident for C. immitis and C. posadasii isolates. In conclusion, current methods for antifungal susceptibility testing yield reproducible profiles for Coccidioides species, which appear to be highly susceptible to most antifungal agents.

Journal of Antimicrobial Chemotherapy, 2005

MMWR. Morbidity and Mortality Weekly Report

F1000 - Post-publication peer review of the biomedical literature, 2011

, 102 conjunctival swab specimens were collected for surveillance purposes from patients across J... more , 102 conjunctival swab specimens were collected for surveillance purposes from patients across Japan suspected of having epidemic keratoconjunctivitis (EKC). Human adenovirus (HAdV) DNA was detected in 61 samples by PCR, though the HAdV type for 6 of the PCR-positive samples could not be determined by phylogenetic analysis using a partial hexon gene sequence. Moreover, for 2 months from January 2009, HAdV strains with identical sequences were isolated from five conjunctival swab samples obtained from EKC patients in five different regions of Japan. For the analyses of the 11 samples mentioned above, we determined the nucleotide sequences of the entire penton base, hexon, and fiber genes and early 3 (E3) region, which are variable regions among HAdV types, and compared them to those of other HAdV species D strains. The nucleotide sequences of loops 1 and 2 in the hexons of all 11 samples showed high degrees of identity with those of the HAdV type 15 (HAdV-15) and HAdV-29 prototype strains. However, the fiber gene and E3 region sequences showed high degrees of identity with those of HAdV-9, and the penton base gene sequence showed a high degree of identity with the penton base gene sequences of HAdV-9 and-26. Moreover, the complete genome sequence of the 2307-S strain, which was isolated by viral culture from 1 of the 11 samples, was determined. The 2307-S strain was a recombinant HAdV between HAdV-9,-15,-26,-29, and/or another HAdV type; however, the recombination sites in the genome were not obvious. We propose that this virus is a novel intertypic recombinant, HAdV-15/29/H9, and may be an etiological agent of EKC.

F1000 - Post-publication peer review of the biomedical literature, 2010

Herpes simplex virus-1 (HSV-1) establishes lifelong latency in peripheral neurons where productiv... more Herpes simplex virus-1 (HSV-1) establishes lifelong latency in peripheral neurons where productive replication is suppressed. While periodic reactivation results in virus production, the molecular basis of neuronal latency remains incompletely understood. Using a primary neuronal culture system of HSV-1 latency and reactivation, we show that continuous signaling through the phosphatidylinositol 3-kinase (PI3-K) pathway triggered by nerve growth factor (NGF)-binding to the TrkA receptor tyrosine kinase (RTK) is instrumental in maintaining latent HSV-1. The PI3-K p110α catalytic subunit, but not the β or δ isoforms, is specifically required to activate 3phosphoinositide-dependent protein kinase-1 (PDK1) and sustain latency. Disrupting this pathway leads to virus reactivation. EGF and GDNF, two other growth factors capable of activating PI3-K and PDK1 but that differ from NGF in their ability to persistently activate Akt, do not fully support HSV-1 latency. Thus the nature of RTK-signaling is a critical host parameter that regulates the HSV-1 latent-lytic switch.

F1000 - Post-publication peer review of the biomedical literature, 2011