Ramendra Das - Academia.edu (original) (raw)

Papers by Ramendra Das

Indian Journal of Animal Sciences, Feb 1, 2022

Indian Journal of Animal Sciences, 2019

The Indian Journal of Animal Sciences

An attempt was made to assess the phenotypic variability and study the management practices of in... more An attempt was made to assess the phenotypic variability and study the management practices of indigenous swamp buffalo 'Bhangor' population from the north-eastern state of Tripura. Bhangor buffalo animals have morphological features and behaviour similar to typical swamp buffalo. A survey was conducted in villages covering entire state, to record the phenotypic information. Adult animals are medium sized with compact body and used for draught and meat purpose. The horn is like sickle shaped (curved) with flat broad base and mostly corrugated, and curved upward to form a circle. Recently, for the first time, we had characterised Bhangor buffalo based on cytogenetic analysis as swamp type showing 2N=48 chromosomes. In present study, we had evaluated the reproductive performance and management practices. The buffalo provide valuable draught power in paddy fields. The indigenous population are important and much needed for the local communities as they are most suitably adapted...

Indian Journal of Animal Research, 2019

Heat shock response (HSR) is a universal mechanism in all organisms upon heat shock (HS) to preve... more Heat shock response (HSR) is a universal mechanism in all organisms upon heat shock (HS) to prevent stress damages. It is under tight regulation by heat shock factors (HSFs) and heat shock proteins (HSPs). On the attenuation of HSR, Heat Shock Factor Binding Protein1 (HSBP1) interacts with HSF1 and thus converting the active trimer form of HSF1 to inert monomer state. Thus, play an important role in regulating heat shock genes. The present study aimed to explore single nucleotide polymorphism (SNP) in HSBP1 and their association with the thermo tolerance traits (respiration rate, rectal temperature and heat tolerance coefficient) for identification of most thermal adapted Murrah buffaloes. Two hundred female Murrah buffaloes were genotyped using custom sequencing to identify SNP. Only one novel SNPs in exon 3 at position g.1892C>T and two nucleotide variation g.1327C>G and g.1202C>T were identified through direct DNA sequence scanning. Homozygous wild type genotypes (CC) we...

African Journal of Biochemistry Research, Sep 30, 2015

Animal Science Reporter, 2015

Conjugated linoleic acid (CLA) collectively refers to a group of positional and geometric isomers... more Conjugated linoleic acid (CLA) collectively refers to a group of positional and geometric isomers of linoleic acid with conjugated double bond structure arranged in different combinations of cis and trans configuration. Importance of CLA in the diets of humans as a vital health promoter has accomplished significance recently. It is present in many foods, especially high in milk and meat of ruminants, has numerous health benefits. Beneficial effects of CLA includes anticarcinogenic, anti-atherogenic, anti-obesity, anti-diabetic, immune system enhancement and few of detrimental affects includes gastrointestinal disorders, insulin resistance and gallstone formation which are isomer specific. Inspite of numerous health benefits associated with CLA, their mechanism of action still remains controversial. So, it is important to understand how different isomers of CLA functioning in the body acts to develop CLA enriched functional foods of animal origin. The objective of this review is to p...

African Journal of Biochemistry Research, 2015

Heat shock proteins play a critical role in the development of thermotolerance and protection fro... more Heat shock proteins play a critical role in the development of thermotolerance and protection from cellular damage associated with heat stresses. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene with thermo-physiological parameters viz, respiration rate (RR), rectal temperature (RT), heat tolerance coefficient (HTC) and total milk yield in Sahiwal cows. The RR and RT were recorded once in different seasons, viz., winter, spring, and summer, at the probable extreme hot hours of the day. Polymorphism of Hsp90ab1 gene, evaluated by comparative sequencing revealed five SNPs, viz., T17871421C, C17871485del, C17872061T, T17872112C and T17872148G. Individuals with CT genotype recorded significantly (P≤0.01) lower RT (°C) than CC genotype in Sahiwal cows. The CT genotype animals also had better production parameter in terms of total milk yield (TMY) (P<0.01). Therefore, our results inferred that CT genotype in Sahiwal cows...

Bhartiya Krishi Anusandhan Patrika, 2021

The domestic water buffalo (Bubalus bubalis) are classified into the swamp and riverine. However,... more The domestic water buffalo (Bubalus bubalis) are classified into the swamp and riverine. However, their hybrids are also found in some parts of Assam (Brahmaputra Valley) in North-east India. Swamp buffaloes have a typical phenotypic appearance, like the shape of horns, small size, and body-color, etc. This study characterizes the indigenous ‘Bhangor’ buffalo population from the Tripura state using cytogenetic analyses. The blood of buffaloes samples were collected across the state, phenotypically identified as swamp buffaloes were cultured, terminated, and harvested using conventional karyotype protocol to determine the number of chromosomes. We have characterized ‘Bhangor’ an indigenous buffalo population from Tripura state using karyotypic analysis for the first time to confirm that all animals phenotypically identified as swamp buffaloes with 2N=48 chromosomes.

Global warming and climate change have become the major threat to the sustainability of livestock... more Global warming and climate change have become the major threat to the sustainability of livestock production systems worldwide. Heat stress produces oxidative stress and affects the alternation of plasma K + and Na + . In the plasma membrane the ion gradients formed by Na + - K + -ATPase enzyme is necessary for Na + - coupled transport of metabolites, nutrients and ions and represents a plausible candidate for heat tolerance traits. ATP1A1 gene encodes for α subunit of Na + - K + - ATPase enzyme, has been mapped on BTA 3 and spans about 22.77 kb and includes 23 exons and coding sequence of 3066 nucleotides. The present study was carried out in 35 Jersey crossbred cows with the objectives to identify SNPs in targeted regions (part of exon 16 to intron 18) of ATP1A1 gene and to analyze their association with respiration rate and rectal temperature. A total of five SNPs (A27008223G, T27008097A, C27008016T, G27008015A and C27007790A) were identified in Jersey crossbred cattle. Respirati...

International Journal of Livestock Research

Indian Journal of Animal Research, May 5, 2018

Early selection of bulls having optimum age at first semen freezing play an important role in imp... more Early selection of bulls having optimum age at first semen freezing play an important role in improving reproductive performance in a dairy herd. Twenty seven years data (1987-2013) on age at first semen freezing (AAFSF), conception rate based on first A.I. (CRFAI) , overall conception rate (OCR) and birth weight (B.WT) of 41 Sahiwal bulls belonging to 8 sets of Sahiwal improvement programme at

Animal Biotechnology

Abstract This study is the maiden attempt for genetic characterization of the Purnathadi, a pheno... more Abstract This study is the maiden attempt for genetic characterization of the Purnathadi, a phenotypically distinct buffalo population of the western Vidarbha region of Maharashtra and to explore genetic diversity using STR markers. A total of 48 unrelated Purnathadi buffaloes from the entire native tract were genotyped using a battery of 25 heterologous microsatellite markers. 5′ end of forward primer of each microsatellite marker was labeled with one of the fluorescent dyes, viz., FAM (Blue), VIC (Green), NED (Yellow) or PET (Red) to assess the fragment length of genotyped PCR product with automated DNA sequencer (ABI 3100). 23 microsatellite loci (except ETH003 and ILSTS030) amplified successfully and adequately high allelic diversity (observed: 0.615 ± 0.043 and expected: 0.655 ± 0.037) was reported with 162 distinct microsatellite alleles. Sufficiently high Shannon index and PIC indicated the suitability of markers to evaluate genetic diversity in Purnathadi buffaloes. Within-population inbreeding estimates (FIS ) for Purnathadi buffalo ranged between −0.171 and 0.495 with global FIS average of 5.9%. The outcome for IAM, TPM and test for mode shift revealed the absence of any recent bottleneck in Purnathadi buffalo. Pairwise FST (genetic differentiation) and gene flow between Purnathadi, Nagpuri and Marathwadi buffaloes were estimated using genotype data of 19 microsatellite markers. Lowest FST (0.031) was observed between Nagpuri and Purnathadi buffaloes with highest gene flow of 7.91% and highest FST (0.094) was between Purnathadi and Marathwadi populations. Present findings will definitely support in designing breeding plan for genetic improvement, as well as for developing conservation strategies of Purnathadi buffalo population. The comparative molecular study with other breeds of the regions is needed.

Indonesian Journal of Biotechnology

Indian milch bovines comprises of 58.56% of total livestock population (512.05 million) in the co... more Indian milch bovines comprises of 58.56% of total livestock population (512.05 million) in the country and primarily includes native and crossbred cattle (37.28%) and water buffaloes (21.28%). Milk and milk products are essential food items of Indian diet especially in children, old and senile. Milk fat is an important constituent of milk and has an economic value and its percentage in milk varies betweem species and breeds within species. Butyrophilin (BTN1A1) a membrane protein regulates secretion of lipids and size of a fat globule in milk. Present study was conducted in 538 bovines of 11 breeds/populations adapted to different parts of India, with an aim to screen and determine the major allele of BTN1A1 gene using PCR-RFLP based test. Results indicate that exon 8 of BTN1A1 gene is polymorphic in Tharparkar, Sahiwal, Jhari and Belahi populations of native cattle and Holstein Friesian and Jersey crossbreds where as the same exon was monomorphic in Murrah, Chilika, Gojri, Chhattis...

International Journal of Bio-resource and Stress Management

production traits may increase an animal's susceptibility to high environmental temperatures (Nar... more production traits may increase an animal's susceptibility to high environmental temperatures (Nardone et al., 2010).

International Journal of Livestock Research

The study was conducted to observe the effect of season on thermoregulation responses. A total of... more The study was conducted to observe the effect of season on thermoregulation responses. A total of 100 cows including 50 Sahiwal and 50 Karan Fries cows were selected from Livestock Research Centre of ICAR-National Dairy Research Institute, Karnal, India. The study was conducted during winter (THI=49.67±1.18), spring (THI=64.65±0.41) and summer (THI=87.28±1.26) seasons. The fluctuations were observed in RR, RT and HTC of Sahiwal and Karan Fries cattle in different seasons. In Sahiwal cows RT (°C) during winter, spring and summer were 37.300±0.095c, 38.178±0.095b and 38.810±0.095a respectively whereas RT (°C) in Karan Fries were 37.492±0.115c, 38.398±0.115b and 39.186±0.115a during winter, spring and summer respectively. RR (breaths min-1) in Sahiwal cows during winter, spring and summer were 15.738±0.795c, 18.158±0.795b and 29.818±0.795a respectively whereas RR in Karan Fries during winter, spring and summer were 15.779±1.136c, 22.979±1.136b and 47.299±1.136a respectively. The magnitude of increase in RR, RT and HTC were found to be higher during summer compared to other seasons in both Sahiwal and Karan Fries cattle. It was observed that Sahiwal cows are less sensitive and are better able to regulate body temperature in response to heat stress than Karan Fries cows.

Indian Journal of Animal Research, 2016

Heat shock protein functions as a molecular chaperone and plays an important role in thermotolera... more Heat shock protein functions as a molecular chaperone and plays an important role in thermotolerance. The study was undertaken to identify single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene by comparative sequencing and to analyze their association with thermo-physiological parameters, viz. respiration rate (RR), rectal temperature (RT) and heat tolerance coefficient (HTC) in different seasons, viz. winter, spring and summer, at probable extreme hours of the day in Sahiwal and Karan Fries cows. The RR, RT and HTC in summers were significantly (P£0.01) higher than the other two seasons. Polymorphism of Hsp90ab1 gene, evaluated by comparative sequencing revealed six SNPs, viz. T17871421C, C17871485del, C17872061T, T17872112C, T17872148G and A17872199C, two each located in exon 8, intron 10 and exon 11. SNP loci T17871421C and C17871485del were found to be monomorphic for allele C and Deletion (-) respectively in experimental population. Individuals with AA genotype showed signifi...

Veterinary World, 2016

The objective of this study was to optimize dry period (DP) length that can maximize the producti... more The objective of this study was to optimize dry period (DP) length that can maximize the production across adjacent lactations and overall lifetime yield. Materials and Methods: Performance records with respect to DP spread over a period of 15-year in Karan Fries (KF) cattle maintained at Livestock Research Centre (National Dairy Research Institute), were collected for the study. Data of 681 KF cows were analyzed by least square technique to examine the effect of non-genetic factors on DP. Season of calving was classified into four seasons: Winter season (December-March), summer season (April-June), rainy season (July-September), and autumn season (October-November);

Indian Journal of Animal Sciences, Feb 1, 2022

Indian Journal of Animal Sciences, 2019

The Indian Journal of Animal Sciences

An attempt was made to assess the phenotypic variability and study the management practices of in... more An attempt was made to assess the phenotypic variability and study the management practices of indigenous swamp buffalo 'Bhangor' population from the north-eastern state of Tripura. Bhangor buffalo animals have morphological features and behaviour similar to typical swamp buffalo. A survey was conducted in villages covering entire state, to record the phenotypic information. Adult animals are medium sized with compact body and used for draught and meat purpose. The horn is like sickle shaped (curved) with flat broad base and mostly corrugated, and curved upward to form a circle. Recently, for the first time, we had characterised Bhangor buffalo based on cytogenetic analysis as swamp type showing 2N=48 chromosomes. In present study, we had evaluated the reproductive performance and management practices. The buffalo provide valuable draught power in paddy fields. The indigenous population are important and much needed for the local communities as they are most suitably adapted...

Indian Journal of Animal Research, 2019

Heat shock response (HSR) is a universal mechanism in all organisms upon heat shock (HS) to preve... more Heat shock response (HSR) is a universal mechanism in all organisms upon heat shock (HS) to prevent stress damages. It is under tight regulation by heat shock factors (HSFs) and heat shock proteins (HSPs). On the attenuation of HSR, Heat Shock Factor Binding Protein1 (HSBP1) interacts with HSF1 and thus converting the active trimer form of HSF1 to inert monomer state. Thus, play an important role in regulating heat shock genes. The present study aimed to explore single nucleotide polymorphism (SNP) in HSBP1 and their association with the thermo tolerance traits (respiration rate, rectal temperature and heat tolerance coefficient) for identification of most thermal adapted Murrah buffaloes. Two hundred female Murrah buffaloes were genotyped using custom sequencing to identify SNP. Only one novel SNPs in exon 3 at position g.1892C>T and two nucleotide variation g.1327C>G and g.1202C>T were identified through direct DNA sequence scanning. Homozygous wild type genotypes (CC) we...

African Journal of Biochemistry Research, Sep 30, 2015

Animal Science Reporter, 2015

Conjugated linoleic acid (CLA) collectively refers to a group of positional and geometric isomers... more Conjugated linoleic acid (CLA) collectively refers to a group of positional and geometric isomers of linoleic acid with conjugated double bond structure arranged in different combinations of cis and trans configuration. Importance of CLA in the diets of humans as a vital health promoter has accomplished significance recently. It is present in many foods, especially high in milk and meat of ruminants, has numerous health benefits. Beneficial effects of CLA includes anticarcinogenic, anti-atherogenic, anti-obesity, anti-diabetic, immune system enhancement and few of detrimental affects includes gastrointestinal disorders, insulin resistance and gallstone formation which are isomer specific. Inspite of numerous health benefits associated with CLA, their mechanism of action still remains controversial. So, it is important to understand how different isomers of CLA functioning in the body acts to develop CLA enriched functional foods of animal origin. The objective of this review is to p...

African Journal of Biochemistry Research, 2015

Heat shock proteins play a critical role in the development of thermotolerance and protection fro... more Heat shock proteins play a critical role in the development of thermotolerance and protection from cellular damage associated with heat stresses. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene with thermo-physiological parameters viz, respiration rate (RR), rectal temperature (RT), heat tolerance coefficient (HTC) and total milk yield in Sahiwal cows. The RR and RT were recorded once in different seasons, viz., winter, spring, and summer, at the probable extreme hot hours of the day. Polymorphism of Hsp90ab1 gene, evaluated by comparative sequencing revealed five SNPs, viz., T17871421C, C17871485del, C17872061T, T17872112C and T17872148G. Individuals with CT genotype recorded significantly (P≤0.01) lower RT (°C) than CC genotype in Sahiwal cows. The CT genotype animals also had better production parameter in terms of total milk yield (TMY) (P<0.01). Therefore, our results inferred that CT genotype in Sahiwal cows...

Bhartiya Krishi Anusandhan Patrika, 2021

The domestic water buffalo (Bubalus bubalis) are classified into the swamp and riverine. However,... more The domestic water buffalo (Bubalus bubalis) are classified into the swamp and riverine. However, their hybrids are also found in some parts of Assam (Brahmaputra Valley) in North-east India. Swamp buffaloes have a typical phenotypic appearance, like the shape of horns, small size, and body-color, etc. This study characterizes the indigenous ‘Bhangor’ buffalo population from the Tripura state using cytogenetic analyses. The blood of buffaloes samples were collected across the state, phenotypically identified as swamp buffaloes were cultured, terminated, and harvested using conventional karyotype protocol to determine the number of chromosomes. We have characterized ‘Bhangor’ an indigenous buffalo population from Tripura state using karyotypic analysis for the first time to confirm that all animals phenotypically identified as swamp buffaloes with 2N=48 chromosomes.

Global warming and climate change have become the major threat to the sustainability of livestock... more Global warming and climate change have become the major threat to the sustainability of livestock production systems worldwide. Heat stress produces oxidative stress and affects the alternation of plasma K + and Na + . In the plasma membrane the ion gradients formed by Na + - K + -ATPase enzyme is necessary for Na + - coupled transport of metabolites, nutrients and ions and represents a plausible candidate for heat tolerance traits. ATP1A1 gene encodes for α subunit of Na + - K + - ATPase enzyme, has been mapped on BTA 3 and spans about 22.77 kb and includes 23 exons and coding sequence of 3066 nucleotides. The present study was carried out in 35 Jersey crossbred cows with the objectives to identify SNPs in targeted regions (part of exon 16 to intron 18) of ATP1A1 gene and to analyze their association with respiration rate and rectal temperature. A total of five SNPs (A27008223G, T27008097A, C27008016T, G27008015A and C27007790A) were identified in Jersey crossbred cattle. Respirati...

International Journal of Livestock Research

Indian Journal of Animal Research, May 5, 2018

Early selection of bulls having optimum age at first semen freezing play an important role in imp... more Early selection of bulls having optimum age at first semen freezing play an important role in improving reproductive performance in a dairy herd. Twenty seven years data (1987-2013) on age at first semen freezing (AAFSF), conception rate based on first A.I. (CRFAI) , overall conception rate (OCR) and birth weight (B.WT) of 41 Sahiwal bulls belonging to 8 sets of Sahiwal improvement programme at

Animal Biotechnology

Abstract This study is the maiden attempt for genetic characterization of the Purnathadi, a pheno... more Abstract This study is the maiden attempt for genetic characterization of the Purnathadi, a phenotypically distinct buffalo population of the western Vidarbha region of Maharashtra and to explore genetic diversity using STR markers. A total of 48 unrelated Purnathadi buffaloes from the entire native tract were genotyped using a battery of 25 heterologous microsatellite markers. 5′ end of forward primer of each microsatellite marker was labeled with one of the fluorescent dyes, viz., FAM (Blue), VIC (Green), NED (Yellow) or PET (Red) to assess the fragment length of genotyped PCR product with automated DNA sequencer (ABI 3100). 23 microsatellite loci (except ETH003 and ILSTS030) amplified successfully and adequately high allelic diversity (observed: 0.615 ± 0.043 and expected: 0.655 ± 0.037) was reported with 162 distinct microsatellite alleles. Sufficiently high Shannon index and PIC indicated the suitability of markers to evaluate genetic diversity in Purnathadi buffaloes. Within-population inbreeding estimates (FIS ) for Purnathadi buffalo ranged between −0.171 and 0.495 with global FIS average of 5.9%. The outcome for IAM, TPM and test for mode shift revealed the absence of any recent bottleneck in Purnathadi buffalo. Pairwise FST (genetic differentiation) and gene flow between Purnathadi, Nagpuri and Marathwadi buffaloes were estimated using genotype data of 19 microsatellite markers. Lowest FST (0.031) was observed between Nagpuri and Purnathadi buffaloes with highest gene flow of 7.91% and highest FST (0.094) was between Purnathadi and Marathwadi populations. Present findings will definitely support in designing breeding plan for genetic improvement, as well as for developing conservation strategies of Purnathadi buffalo population. The comparative molecular study with other breeds of the regions is needed.

Indonesian Journal of Biotechnology

Indian milch bovines comprises of 58.56% of total livestock population (512.05 million) in the co... more Indian milch bovines comprises of 58.56% of total livestock population (512.05 million) in the country and primarily includes native and crossbred cattle (37.28%) and water buffaloes (21.28%). Milk and milk products are essential food items of Indian diet especially in children, old and senile. Milk fat is an important constituent of milk and has an economic value and its percentage in milk varies betweem species and breeds within species. Butyrophilin (BTN1A1) a membrane protein regulates secretion of lipids and size of a fat globule in milk. Present study was conducted in 538 bovines of 11 breeds/populations adapted to different parts of India, with an aim to screen and determine the major allele of BTN1A1 gene using PCR-RFLP based test. Results indicate that exon 8 of BTN1A1 gene is polymorphic in Tharparkar, Sahiwal, Jhari and Belahi populations of native cattle and Holstein Friesian and Jersey crossbreds where as the same exon was monomorphic in Murrah, Chilika, Gojri, Chhattis...

International Journal of Bio-resource and Stress Management

production traits may increase an animal's susceptibility to high environmental temperatures (Nar... more production traits may increase an animal's susceptibility to high environmental temperatures (Nardone et al., 2010).

International Journal of Livestock Research

The study was conducted to observe the effect of season on thermoregulation responses. A total of... more The study was conducted to observe the effect of season on thermoregulation responses. A total of 100 cows including 50 Sahiwal and 50 Karan Fries cows were selected from Livestock Research Centre of ICAR-National Dairy Research Institute, Karnal, India. The study was conducted during winter (THI=49.67±1.18), spring (THI=64.65±0.41) and summer (THI=87.28±1.26) seasons. The fluctuations were observed in RR, RT and HTC of Sahiwal and Karan Fries cattle in different seasons. In Sahiwal cows RT (°C) during winter, spring and summer were 37.300±0.095c, 38.178±0.095b and 38.810±0.095a respectively whereas RT (°C) in Karan Fries were 37.492±0.115c, 38.398±0.115b and 39.186±0.115a during winter, spring and summer respectively. RR (breaths min-1) in Sahiwal cows during winter, spring and summer were 15.738±0.795c, 18.158±0.795b and 29.818±0.795a respectively whereas RR in Karan Fries during winter, spring and summer were 15.779±1.136c, 22.979±1.136b and 47.299±1.136a respectively. The magnitude of increase in RR, RT and HTC were found to be higher during summer compared to other seasons in both Sahiwal and Karan Fries cattle. It was observed that Sahiwal cows are less sensitive and are better able to regulate body temperature in response to heat stress than Karan Fries cows.

Indian Journal of Animal Research, 2016

Heat shock protein functions as a molecular chaperone and plays an important role in thermotolera... more Heat shock protein functions as a molecular chaperone and plays an important role in thermotolerance. The study was undertaken to identify single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene by comparative sequencing and to analyze their association with thermo-physiological parameters, viz. respiration rate (RR), rectal temperature (RT) and heat tolerance coefficient (HTC) in different seasons, viz. winter, spring and summer, at probable extreme hours of the day in Sahiwal and Karan Fries cows. The RR, RT and HTC in summers were significantly (P£0.01) higher than the other two seasons. Polymorphism of Hsp90ab1 gene, evaluated by comparative sequencing revealed six SNPs, viz. T17871421C, C17871485del, C17872061T, T17872112C, T17872148G and A17872199C, two each located in exon 8, intron 10 and exon 11. SNP loci T17871421C and C17871485del were found to be monomorphic for allele C and Deletion (-) respectively in experimental population. Individuals with AA genotype showed signifi...

Veterinary World, 2016

The objective of this study was to optimize dry period (DP) length that can maximize the producti... more The objective of this study was to optimize dry period (DP) length that can maximize the production across adjacent lactations and overall lifetime yield. Materials and Methods: Performance records with respect to DP spread over a period of 15-year in Karan Fries (KF) cattle maintained at Livestock Research Centre (National Dairy Research Institute), were collected for the study. Data of 681 KF cows were analyzed by least square technique to examine the effect of non-genetic factors on DP. Season of calving was classified into four seasons: Winter season (December-March), summer season (April-June), rainy season (July-September), and autumn season (October-November);