Ramses Forsyth - Academia.edu (original) (raw)

Papers by Ramses Forsyth

PubMed, Dec 1, 2003

Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release ... more Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release of sensory neuropeptides Substance P (SP) and Calcitonin Gene-Related Peptide (CGRP) by the peripheral endings of these ultra-thin nerve fibres. These neuropeptides have, among others, a vasodilatory effect, which explains why they play a role in the healing of soft tissues. Burst TENS (Transcutaneous Electric Nerve Stimulation) is known to be most effective in influencing C fibre-evoked activity. This is why burst TENS was used in a randomised study as a stimulus for the healing of the sutured Achilles tendon in 10 patients, versus 10 others who received no stimulus. There was one drop-out in each group, so that 2 x 9 patients remained available for the study. A needle biopsy, performed after six weeks, showed no significant influence of burst TENS on the histological healing stage, as compared with a rat study. However, a semi-quantitative evaluation of the number of fibroblasts showed a significant advantage for the stimulated group: p = 0.007. This means that burst TENS might influence healing of Achilles tendon sutures in man. But above all, it means that a histochemical study of the influence of burst TENS on the release of substance P and CGRP, after suture of the Achilles tendon in man, would be worthwhile.

PubMed, Jun 1, 2005

Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release ... more Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release of sensory neuropeptides, such as substance P (SP), by the peripheral endings of sensory unmyelinated C nerve fibres. These neuropeptides play a role in the healing of soft tissues. Burst TENS (Transcutaneous Electric Nerve Stimulation) is known to be most effective in influencing retrograde C fibre-evoked activity. This is why burst TENS was used in a randomised study as a stimulus for the healing of the sutured Achilles tendon in 9 patients, versus 9 others who received no stimulus. Originally, each group consisted of 10 patients, but there was a single drop-out in each group. Six weeks after surgery a needle biopsy sample was obtained, and stained with Movat's pentachrome stain. It showed a statistically significant influence of burst TENS on new collagen production, maturation of newly formed collagen and organisation of collagen. This suggests that burst TENS might positively influence healing of Achilles tendon suture in man.

Anatomia Histologia Embryologia, Oct 8, 2012

Foot & Ankle International, Oct 1, 2005

In the last few years much research has been conducted in methods to promote tendon healing. The ... more In the last few years much research has been conducted in methods to promote tendon healing. The aim of this study was to determine if the healing process after operative repair of rat Achilles tendons could be stimulated by the paratendinous injection of a sensory peptide, substance P (SP). Ninety-six male Sprague-Dawley rats were randomly allocated to four groups: (I) control buffer injections, (II) injections of SP 10(-6) mol/kg body weight combined with a carrier, (III) injections of SP 10(-8) mol/kg BW with the carrier, and (IV) injections with the carrier only (thiorphan 1 micromol/kg BW and captopril 30 micromol/kg BW, both neutral endopeptidase inhibitors). The influence on tissue repair was determined from the histologic measurement of fibroblast proliferation, angiogenesis, and collagen organization. On days 7, 14, 28 and 42, animals were sacrificed and histologic evaluations were performed on the injured Achilles tendon constructs. The two groups subjected to SP injections showed a significant initial fibroblast proliferation on day 7 (p < 0.05), which rapidly declined by day 14 to the level of cellular proliferation observed with the use of thiorphan and captopril. Capillary proliferation showed a similar evolution, except that in the second week angiogenesis in the treated groups was below the level of the control group. Strikingly, collagen orientation increased faster in the groups injected with SP. This was obvious from the second week already and the difference remained until the completion of the study. This is the first study to demonstrate that paratendinous injections of SP after operative repair of the Achilles tendon in rats appears to provide a boost to the initial stages of healing and significantly accelerate the reparative phase of the healing process.

Cancers

In recent years, there has been an increasing scientific interest in the interaction between anae... more In recent years, there has been an increasing scientific interest in the interaction between anaesthesia and cancer development. Retrospective studies show that the choice of anaesthetics may influence cancer outcome and cancer recurrence; however, these studies show contradictory results. Recently, some large randomized clinical trials have been completed, yet they show no significant effect of anaesthetics on cancer outcomes. In this scoping review, we compiled a body of in vivo and in vitro studies with the goal of evaluating the biological effects of anaesthetics on cancer cells in comparison to clinical effects as described in recent studies. It was found that sevoflurane, propofol, opioids and lidocaine are likely to display direct biological effects on cancer cells; however, significant effects are only found in studies with exposure to high concentrations of anaesthetics and/or during longer exposure times. When compared to clinical data, these differences in exposure and do...

The International Journal of Cardiovascular Imaging

Journal for ImmunoTherapy of Cancer

BackgroundIntratumoral (IT) myeloid dendritic cells (myDCs) play a pivotal role in initiating ant... more BackgroundIntratumoral (IT) myeloid dendritic cells (myDCs) play a pivotal role in initiating antitumor immune responses and relicensing of anti-tumor cytotoxic T lymphocytes within the tumor microenvironment. Talimogene laherparepvec (T-VEC) induces immunogenic cell death, thereby providing maturation signals and enhancing the release of tumor antigens that can be captured and processed by CD1c (BDCA-1)+ / CD141 (BDCA-3)+ myDCs, in order to reinvigorate the cancer-immunity cycle.MethodsIn this phase I trial, patients with advanced melanoma who failed standard therapy were eligible for IT injections of ≥1 non-visceral metastases with T-VEC on day 1 followed by IT injection of CD1c (BDCA-1)+ myDCs +/- CD141 (BDCA-3)+ myDCs on day 2. T-VEC injections were repeated on day 21 and every 14 days thereafter. The number of IT administered CD1c (BDCA-1)+ myDCs was escalated from 0.5×106, to 1×106, to a maximum of 10×106 cells in three sequential cohorts. In cohort 4, all isolated CD1c (BDCA-...

Neuro-Oncology

Background Perioperative intracerebral (iCE) administration of ipilimumab (IPI) and nivolumab (NI... more Background Perioperative intracerebral (iCE) administration of ipilimumab (IPI) and nivolumab (NIVO) in combination with IV NIVO was shown to be feasible, safe and associated with an encouraging survival benefit (Duerinck et al. JITC 2021). In subsequent cohorts, combination of iCE administration with biweekly intracavitary (iCA, via an Ommaya reservoir) administration of increasing doses of IPI and NIVO was investigated. Methods Three cohorts were defined according to resectability and postoperative treatment schedule. Patients (pts) in cohort-A and -C underwent a maximal safe resection, pts in cohort-B stereotactic biopsy only. All pts received iCE administration of 10 mg NIVO and 5 mg IPI at the end of the surgical intervention, after which an OR was implanted and an additional 10mg of NIVO and IPI (1, 5 or 10 mg) was administered iCA in cohort-C. All pts received biweekly postoperative NIVO 10 mg IV and iCA administrations of NIVO (3 dose levels were investigated in cohorts-A an...

BMC Cancer, 2022

Background The detection of suspicious microcalcifications on mammography represents one of the e... more Background The detection of suspicious microcalcifications on mammography represents one of the earliest signs of a malignant breast tumor. Assessing microcalcifications’ characteristics based on their appearance on 2D breast imaging modalities is in many cases challenging for radiologists. The aims of this study were to: (a) analyse the association of shape and texture properties of breast microcalcifications (extracted by scanning breast tissue with a high resolution 3D scanner) with malignancy, (b) evaluate microcalcifications’ potential to diagnose benign/malignant patients. Methods Biopsy samples of 94 female patients with suspicious microcalcifications detected during a mammography, were scanned using a micro-CT scanner at a resolution of 9 μm. Several preprocessing techniques were applied on 3504 extracted microcalcifications. A high amount of radiomic features were extracted in an attempt to capture differences among microcalcifications occurring in benign and malignant lesi...

Journal of Thoracic Oncology, 2012

International Journal of Cancer, 2021

In non‐small cell lung cancer (NSCLC), activating mutations in the epidermal growth factor recept... more In non‐small cell lung cancer (NSCLC), activating mutations in the epidermal growth factor receptor (EGFR) induce sensitivity to EGFR tyrosine kinase inhibitors. Despite impressive clinical responses, patients ultimately relapse as a reservoir of drug‐tolerant cells persist, which ultimately leads to acquired resistance mechanisms. We performed an unbiased high‐throughput siRNA screen to identify proteins that abrogate the response of EGFR‐mutant NSCLC to EGFR‐targeted therapy. The deubiquitinase USP13 was a top hit resulting from this screen. Targeting USP13 increases the sensitivity to EGFR inhibition with small molecules in vitro and in vivo. USP13 selectively stabilizes mutant EGFR in a peptidase‐independent manner by counteracting the action of members of the Cbl family of E3 ubiquitin ligases. We conclude that USP13 is a strong mutant EGFR‐specific cotarget that could improve the treatment efficacy of EGFR‐targeted therapies.

The Netherlands journal of medicine, 2018

Langerhans cell histiocytosis (LCH) is a rare disorder, characterised by a monoclonal proliferati... more Langerhans cell histiocytosis (LCH) is a rare disorder, characterised by a monoclonal proliferation of aberrant histiocytes that accumulate in and infiltrate into different organs. When the hypothalamic-pituitary axis is involved, central diabetes insipidus (CDI) can be its first manifestation. Three cases of LCH with central diabetes insipidus were retrospectively analyzed: Case 1 is a 41-year old female presenting with polyuria and polydipsia. Diabetes insipidus was diagnosed and treated with desmopressin. MRI pituitary showed hypophysitis. Subsequently, she developed bone lesions and a biopsy demonstrated LCH. Case 2 is a 51-year old female presenting in 2009 with polyuria and polydipsia. Diabetes insipidus was diagnosed and treated with desmopressin. MRI pituitary revealed hypophysitis. LCH was suspected because of known pulmonary histiocytosis. Coexisting bone lesions were biopsied and confirmed LCH. Case 3 is a 44-year old female presenting with diabetes insipidus. She was tre...

Journal of Neuropathology & Experimental Neurology, 2021

Aesthetic Surgery Journal, 2020

Background Despite the popularity of hyaluronic acid (HA) filler treatments, few publications foc... more Background Despite the popularity of hyaluronic acid (HA) filler treatments, few publications focus on their effects on adipose tissue. Objectives The authors assessed the deposition pattern in the subcutis of injected HA, the tissue response at short and intermediate term, and the effects of remodeling the filler by strong finger pressure immediately after the treatment. Methods Two brands, specifically developed by the industry for deep injection, were compared. The gels were injected subcutaneously in 5 candidates for abdominoplasty or breast reduction, in the area of excision, 6 to 98 days before surgery. Ultrasound measurements and films were compared with postoperative histological findings. Tissue response was scored semi-quantitatively. Results Real-time ultrasound showed a slightly different deposition pattern of the 2 brands. Histologically, both were present in large pools of the same magnitude and looked the same. Linear retrograde injection sometimes resulted in a globu...

European Journal of Radiology, 2019

Purpose: To perform an MR (magnetic resonance) imaging, anatomical, and histological evaluation o... more Purpose: To perform an MR (magnetic resonance) imaging, anatomical, and histological evaluation of the abdominal muscles and adductor tendon insertions Method: Four fresh cadaveric pelvis specimens were imaged at 3T with the following imaging parameters: TE (echo time)/TR (repetition time): 20 4090, slice thickness: 2 mm, FOV: 270 x 90, matrix size: 512. Anatomical slices were obtained with a band saw and photographed. MR images and photographs were evaluated by an anatomist and radiologist. Selected 3 mm thick slices were placed in formalin and decalcified, cut, placed on large slides, and stained with hematoxylin eosin stain (HES). Results: The main adductor tendon insertions are: the anterosuperior aspect of the pubic bone for the adductor longus, the anteroinferior aspect of the pubic bone-for the adductor brevis, and the inferior aspect of the pubic bone for the adductor magnus. On histology, the adductor longus tendon fibers inserted perpendicularly into the bone and the fibrocartilage enthesis and cross connected along the anterior pubic ligament into the controlateral tendon. The rectus abdominis-pyramidalis unit was covered by a thin anterior and posterior aponeurosis. The posterior aponeurosis inserted into the superior aspect of the anterior pubic ligament, whereas the anterior aponeurosis fused distally with the adductor longus tendons. Conclusion: Our findings demonstrate the insertions of the adductor tenodnson the pubic ligament ad pubic bone.Histologically, the adductor longus tendon fibers inserted perpendicularly into the bone through a fibrocartilage enthesis, and cross connected along the anterior pubic ligament into the contralateral tendon.

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 ... more This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence.

Virchows Archiv, 2014

Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and... more Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and occasionally metastatic disease which are unpredictable by morphology. Additional routinely usable biomarkers do not exist. Gene expression profiles of six clinically defined groups of GCTB and one group of aneurysmal bone cyst (ABC) were determined by microarray (n=33). The most promising differentially expressed genes were validated by Q-PCR as potential biomarkers in a larger patient group (n=41). Corresponding protein expression was confirmed by immunohistochemistry. Unsupervised hierarchical clustering reveals a metastatic GCTB cluster, a heterogeneous, non-metastatic GCTB cluster, and a primary ABC cluster. Balanced score testing indicates that lumican (LUM) and decorin (DCN) are the most promising biomarkers as they have lower level of expression in the metastatic group. Expression of dermatopontin (DPT) was significantly lower in recurrent tumors. Validation of the results was performed by paired and unpaired t test in primary

PubMed, Dec 1, 2003

Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release ... more Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release of sensory neuropeptides Substance P (SP) and Calcitonin Gene-Related Peptide (CGRP) by the peripheral endings of these ultra-thin nerve fibres. These neuropeptides have, among others, a vasodilatory effect, which explains why they play a role in the healing of soft tissues. Burst TENS (Transcutaneous Electric Nerve Stimulation) is known to be most effective in influencing C fibre-evoked activity. This is why burst TENS was used in a randomised study as a stimulus for the healing of the sutured Achilles tendon in 10 patients, versus 10 others who received no stimulus. There was one drop-out in each group, so that 2 x 9 patients remained available for the study. A needle biopsy, performed after six weeks, showed no significant influence of burst TENS on the histological healing stage, as compared with a rat study. However, a semi-quantitative evaluation of the number of fibroblasts showed a significant advantage for the stimulated group: p = 0.007. This means that burst TENS might influence healing of Achilles tendon sutures in man. But above all, it means that a histochemical study of the influence of burst TENS on the release of substance P and CGRP, after suture of the Achilles tendon in man, would be worthwhile.

PubMed, Jun 1, 2005

Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release ... more Retrograde or antidromic stimulation of the nociceptive C fibres is known to lead to the release of sensory neuropeptides, such as substance P (SP), by the peripheral endings of sensory unmyelinated C nerve fibres. These neuropeptides play a role in the healing of soft tissues. Burst TENS (Transcutaneous Electric Nerve Stimulation) is known to be most effective in influencing retrograde C fibre-evoked activity. This is why burst TENS was used in a randomised study as a stimulus for the healing of the sutured Achilles tendon in 9 patients, versus 9 others who received no stimulus. Originally, each group consisted of 10 patients, but there was a single drop-out in each group. Six weeks after surgery a needle biopsy sample was obtained, and stained with Movat's pentachrome stain. It showed a statistically significant influence of burst TENS on new collagen production, maturation of newly formed collagen and organisation of collagen. This suggests that burst TENS might positively influence healing of Achilles tendon suture in man.

Anatomia Histologia Embryologia, Oct 8, 2012

Foot & Ankle International, Oct 1, 2005

In the last few years much research has been conducted in methods to promote tendon healing. The ... more In the last few years much research has been conducted in methods to promote tendon healing. The aim of this study was to determine if the healing process after operative repair of rat Achilles tendons could be stimulated by the paratendinous injection of a sensory peptide, substance P (SP). Ninety-six male Sprague-Dawley rats were randomly allocated to four groups: (I) control buffer injections, (II) injections of SP 10(-6) mol/kg body weight combined with a carrier, (III) injections of SP 10(-8) mol/kg BW with the carrier, and (IV) injections with the carrier only (thiorphan 1 micromol/kg BW and captopril 30 micromol/kg BW, both neutral endopeptidase inhibitors). The influence on tissue repair was determined from the histologic measurement of fibroblast proliferation, angiogenesis, and collagen organization. On days 7, 14, 28 and 42, animals were sacrificed and histologic evaluations were performed on the injured Achilles tendon constructs. The two groups subjected to SP injections showed a significant initial fibroblast proliferation on day 7 (p < 0.05), which rapidly declined by day 14 to the level of cellular proliferation observed with the use of thiorphan and captopril. Capillary proliferation showed a similar evolution, except that in the second week angiogenesis in the treated groups was below the level of the control group. Strikingly, collagen orientation increased faster in the groups injected with SP. This was obvious from the second week already and the difference remained until the completion of the study. This is the first study to demonstrate that paratendinous injections of SP after operative repair of the Achilles tendon in rats appears to provide a boost to the initial stages of healing and significantly accelerate the reparative phase of the healing process.

Cancers

In recent years, there has been an increasing scientific interest in the interaction between anae... more In recent years, there has been an increasing scientific interest in the interaction between anaesthesia and cancer development. Retrospective studies show that the choice of anaesthetics may influence cancer outcome and cancer recurrence; however, these studies show contradictory results. Recently, some large randomized clinical trials have been completed, yet they show no significant effect of anaesthetics on cancer outcomes. In this scoping review, we compiled a body of in vivo and in vitro studies with the goal of evaluating the biological effects of anaesthetics on cancer cells in comparison to clinical effects as described in recent studies. It was found that sevoflurane, propofol, opioids and lidocaine are likely to display direct biological effects on cancer cells; however, significant effects are only found in studies with exposure to high concentrations of anaesthetics and/or during longer exposure times. When compared to clinical data, these differences in exposure and do...

The International Journal of Cardiovascular Imaging

Journal for ImmunoTherapy of Cancer

BackgroundIntratumoral (IT) myeloid dendritic cells (myDCs) play a pivotal role in initiating ant... more BackgroundIntratumoral (IT) myeloid dendritic cells (myDCs) play a pivotal role in initiating antitumor immune responses and relicensing of anti-tumor cytotoxic T lymphocytes within the tumor microenvironment. Talimogene laherparepvec (T-VEC) induces immunogenic cell death, thereby providing maturation signals and enhancing the release of tumor antigens that can be captured and processed by CD1c (BDCA-1)+ / CD141 (BDCA-3)+ myDCs, in order to reinvigorate the cancer-immunity cycle.MethodsIn this phase I trial, patients with advanced melanoma who failed standard therapy were eligible for IT injections of ≥1 non-visceral metastases with T-VEC on day 1 followed by IT injection of CD1c (BDCA-1)+ myDCs +/- CD141 (BDCA-3)+ myDCs on day 2. T-VEC injections were repeated on day 21 and every 14 days thereafter. The number of IT administered CD1c (BDCA-1)+ myDCs was escalated from 0.5×106, to 1×106, to a maximum of 10×106 cells in three sequential cohorts. In cohort 4, all isolated CD1c (BDCA-...

Neuro-Oncology

Background Perioperative intracerebral (iCE) administration of ipilimumab (IPI) and nivolumab (NI... more Background Perioperative intracerebral (iCE) administration of ipilimumab (IPI) and nivolumab (NIVO) in combination with IV NIVO was shown to be feasible, safe and associated with an encouraging survival benefit (Duerinck et al. JITC 2021). In subsequent cohorts, combination of iCE administration with biweekly intracavitary (iCA, via an Ommaya reservoir) administration of increasing doses of IPI and NIVO was investigated. Methods Three cohorts were defined according to resectability and postoperative treatment schedule. Patients (pts) in cohort-A and -C underwent a maximal safe resection, pts in cohort-B stereotactic biopsy only. All pts received iCE administration of 10 mg NIVO and 5 mg IPI at the end of the surgical intervention, after which an OR was implanted and an additional 10mg of NIVO and IPI (1, 5 or 10 mg) was administered iCA in cohort-C. All pts received biweekly postoperative NIVO 10 mg IV and iCA administrations of NIVO (3 dose levels were investigated in cohorts-A an...

BMC Cancer, 2022

Background The detection of suspicious microcalcifications on mammography represents one of the e... more Background The detection of suspicious microcalcifications on mammography represents one of the earliest signs of a malignant breast tumor. Assessing microcalcifications’ characteristics based on their appearance on 2D breast imaging modalities is in many cases challenging for radiologists. The aims of this study were to: (a) analyse the association of shape and texture properties of breast microcalcifications (extracted by scanning breast tissue with a high resolution 3D scanner) with malignancy, (b) evaluate microcalcifications’ potential to diagnose benign/malignant patients. Methods Biopsy samples of 94 female patients with suspicious microcalcifications detected during a mammography, were scanned using a micro-CT scanner at a resolution of 9 μm. Several preprocessing techniques were applied on 3504 extracted microcalcifications. A high amount of radiomic features were extracted in an attempt to capture differences among microcalcifications occurring in benign and malignant lesi...

Journal of Thoracic Oncology, 2012

International Journal of Cancer, 2021

In non‐small cell lung cancer (NSCLC), activating mutations in the epidermal growth factor recept... more In non‐small cell lung cancer (NSCLC), activating mutations in the epidermal growth factor receptor (EGFR) induce sensitivity to EGFR tyrosine kinase inhibitors. Despite impressive clinical responses, patients ultimately relapse as a reservoir of drug‐tolerant cells persist, which ultimately leads to acquired resistance mechanisms. We performed an unbiased high‐throughput siRNA screen to identify proteins that abrogate the response of EGFR‐mutant NSCLC to EGFR‐targeted therapy. The deubiquitinase USP13 was a top hit resulting from this screen. Targeting USP13 increases the sensitivity to EGFR inhibition with small molecules in vitro and in vivo. USP13 selectively stabilizes mutant EGFR in a peptidase‐independent manner by counteracting the action of members of the Cbl family of E3 ubiquitin ligases. We conclude that USP13 is a strong mutant EGFR‐specific cotarget that could improve the treatment efficacy of EGFR‐targeted therapies.

The Netherlands journal of medicine, 2018

Langerhans cell histiocytosis (LCH) is a rare disorder, characterised by a monoclonal proliferati... more Langerhans cell histiocytosis (LCH) is a rare disorder, characterised by a monoclonal proliferation of aberrant histiocytes that accumulate in and infiltrate into different organs. When the hypothalamic-pituitary axis is involved, central diabetes insipidus (CDI) can be its first manifestation. Three cases of LCH with central diabetes insipidus were retrospectively analyzed: Case 1 is a 41-year old female presenting with polyuria and polydipsia. Diabetes insipidus was diagnosed and treated with desmopressin. MRI pituitary showed hypophysitis. Subsequently, she developed bone lesions and a biopsy demonstrated LCH. Case 2 is a 51-year old female presenting in 2009 with polyuria and polydipsia. Diabetes insipidus was diagnosed and treated with desmopressin. MRI pituitary revealed hypophysitis. LCH was suspected because of known pulmonary histiocytosis. Coexisting bone lesions were biopsied and confirmed LCH. Case 3 is a 44-year old female presenting with diabetes insipidus. She was tre...

Journal of Neuropathology & Experimental Neurology, 2021

Aesthetic Surgery Journal, 2020

Background Despite the popularity of hyaluronic acid (HA) filler treatments, few publications foc... more Background Despite the popularity of hyaluronic acid (HA) filler treatments, few publications focus on their effects on adipose tissue. Objectives The authors assessed the deposition pattern in the subcutis of injected HA, the tissue response at short and intermediate term, and the effects of remodeling the filler by strong finger pressure immediately after the treatment. Methods Two brands, specifically developed by the industry for deep injection, were compared. The gels were injected subcutaneously in 5 candidates for abdominoplasty or breast reduction, in the area of excision, 6 to 98 days before surgery. Ultrasound measurements and films were compared with postoperative histological findings. Tissue response was scored semi-quantitatively. Results Real-time ultrasound showed a slightly different deposition pattern of the 2 brands. Histologically, both were present in large pools of the same magnitude and looked the same. Linear retrograde injection sometimes resulted in a globu...

European Journal of Radiology, 2019

Purpose: To perform an MR (magnetic resonance) imaging, anatomical, and histological evaluation o... more Purpose: To perform an MR (magnetic resonance) imaging, anatomical, and histological evaluation of the abdominal muscles and adductor tendon insertions Method: Four fresh cadaveric pelvis specimens were imaged at 3T with the following imaging parameters: TE (echo time)/TR (repetition time): 20 4090, slice thickness: 2 mm, FOV: 270 x 90, matrix size: 512. Anatomical slices were obtained with a band saw and photographed. MR images and photographs were evaluated by an anatomist and radiologist. Selected 3 mm thick slices were placed in formalin and decalcified, cut, placed on large slides, and stained with hematoxylin eosin stain (HES). Results: The main adductor tendon insertions are: the anterosuperior aspect of the pubic bone for the adductor longus, the anteroinferior aspect of the pubic bone-for the adductor brevis, and the inferior aspect of the pubic bone for the adductor magnus. On histology, the adductor longus tendon fibers inserted perpendicularly into the bone and the fibrocartilage enthesis and cross connected along the anterior pubic ligament into the controlateral tendon. The rectus abdominis-pyramidalis unit was covered by a thin anterior and posterior aponeurosis. The posterior aponeurosis inserted into the superior aspect of the anterior pubic ligament, whereas the anterior aponeurosis fused distally with the adductor longus tendons. Conclusion: Our findings demonstrate the insertions of the adductor tenodnson the pubic ligament ad pubic bone.Histologically, the adductor longus tendon fibers inserted perpendicularly into the bone through a fibrocartilage enthesis, and cross connected along the anterior pubic ligament into the contralateral tendon.

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 ... more This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence.

Virchows Archiv, 2014

Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and... more Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and occasionally metastatic disease which are unpredictable by morphology. Additional routinely usable biomarkers do not exist. Gene expression profiles of six clinically defined groups of GCTB and one group of aneurysmal bone cyst (ABC) were determined by microarray (n=33). The most promising differentially expressed genes were validated by Q-PCR as potential biomarkers in a larger patient group (n=41). Corresponding protein expression was confirmed by immunohistochemistry. Unsupervised hierarchical clustering reveals a metastatic GCTB cluster, a heterogeneous, non-metastatic GCTB cluster, and a primary ABC cluster. Balanced score testing indicates that lumican (LUM) and decorin (DCN) are the most promising biomarkers as they have lower level of expression in the metastatic group. Expression of dermatopontin (DPT) was significantly lower in recurrent tumors. Validation of the results was performed by paired and unpaired t test in primary