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Papers by Ramshanker Ramanathan

Research paper thumbnail of Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer

International Journal of Cancer, Jan 28, 2011

Research paper thumbnail of Review for "Effect of cilostazol on carotid plaque volume measured by <scp>3D</scp> ultrasound in patients with type 2 diabetes: <scp>FANCY</scp> study

Research paper thumbnail of P228Association of vessel wall changes with cardiovascular risk markers in healthy individuals

Cardiovascular Research, Jun 27, 2014

Objective: To characterize subclinical atherosclerosis in healthy, non-medicated middle-aged indi... more Objective: To characterize subclinical atherosclerosis in healthy, non-medicated middle-aged individuals and to compare these findings with classical cardiovascular risk factors and biochemical markers of inflammation and endothelial dysfunction. Methods: Participants were recruited from the Danish Risk Score Study. A total of 277 individuals, 66% of the invited, aged 50 or 60 years, without known cardiovascular disease or diabetes were randomly selected from national registries. Persons who did not take any medication represented the study population, N = 209. The population was separated in two groups according to coronary artery calcification (CAC) by non-enhanced cardiac-CT, and carotid plaque by ultrasound. Inflammation (C-reactive protein (CRP), fibrinogen and D-dimer) and endothelial dysfunction (von Willebrand factor, tissueplasminogen activator (t-PA), and plasminogen activator inhibitor-1 (PAI-1)) were studied. Results: Coronary calcification was seen in 39 % while carotid plaque was observed in 28 % of individuals. Age, gender, smoking, blood pressure and triglycerides were significantly associated with CAC (P , 0.05), while age and smoking were significantly associated with carotid plaque (P , 0.005).The concentrations of t-PA and PAI-1 were significantly associated with CAC (P , 0.01), whereas fibrinogen levels were associated with plaque (P , 0.05). Logistic multivariate regression revealed that age, gender, systolic blood pressure and total cholesterol (P , 0.1) were associated with CAC, while no associations were found with carotid plaque. Conclusion: Even in apparantly healthy middle-aged individuals manifest morphological and biochemical signs of subclinical atherosclerosis were observed in more than 50 %. Markers of inflammation and endothelial dysfunction were associated with subclinical atherosclerosis in asymptomatic healthy individuals, but these changes seemed to be mediated through classical risk factors. Furthermore, we demonstrate that classical risk factors and biochemical markers are associated differently with atherosclerotic lesions depending on the vascular location.

Research paper thumbnail of Sex difference in clot lysability and association to coronary artery calcification

Biology of Sex Differences, Feb 13, 2018

Background: Incidence and prevalence of cardiovascular disease (CVD) differ between sexes, and wo... more Background: Incidence and prevalence of cardiovascular disease (CVD) differ between sexes, and women experience CVD later than men. Changes in fibrin clot lysability are associated with CVD, and the present study addresses sex differences in fibrin clot lysability in asymptomatic middle-aged individuals and the relation to coronary artery calcification (CAC). Methods: Participants free of morbidities and medication, N = 163, were randomly chosen from a national registry among citizens, 50 or 60 years of age, and were followed for 5 years. CAC was determined by the Agatston (Ag) score both at baseline and at follow-up. Based on the changes in Ag, the population was divided into two groups: ΔAg = 0 U or ΔAg > 0 U. Fibrin clot analyses were based on turbidimetric methods. Results: At baseline, 116 women and 97 men were included; 84 women and 79 men completed the 5-year follow-up (77%). Independently of covariates, women with ΔAg > 0 had reduced mean (SD) fibrin lysability at follow-up, 40.2% (15.9), both in comparison to baseline, 47.8% (20.4), p = 0.001, to women with ΔAg = 0 U, 51. 2% (24.5), p = 0.028, and to men with ΔAg > 0 U, 54.4% (21.0), p = 0.002. Conclusions: Fibrin clot lysability changes over time with considerable sex differences. Women with progression of CAC have reduced fibrin clot lysability compared to men, indicating a sex-specific association between morphological vessel wall changes and fibrin clot lysability.

Research paper thumbnail of Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 5 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Targets down-regulated after 24 h Targets down-regulated after 48 h

Research paper thumbnail of Supplementary Table 3 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Figure 3 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Supplementary Fig. S3. Real time RT-PCR validation of miR-21 transfection efficacy (above) and mi... more Supplementary Fig. S3. Real time RT-PCR validation of miR-21 transfection efficacy (above) and miR-21 target regulation (below). PCR validation of miR-21 regulation 6, 16 and 24 hours following transfection of T24 cells with either a miR-21 precursor or a miR-21 LNA knockdown probe. Expression levels are normalized by comparing to the expression level of miR-21 when transfecting with a scrambled sequence. The fold changes on the y-axis are log2 transformed.

Research paper thumbnail of Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Data from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associat... more Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associat... more Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer

International Journal of Cancer, Jan 28, 2011

Research paper thumbnail of Review for "Effect of cilostazol on carotid plaque volume measured by <scp>3D</scp> ultrasound in patients with type 2 diabetes: <scp>FANCY</scp> study

Research paper thumbnail of P228Association of vessel wall changes with cardiovascular risk markers in healthy individuals

Cardiovascular Research, Jun 27, 2014

Objective: To characterize subclinical atherosclerosis in healthy, non-medicated middle-aged indi... more Objective: To characterize subclinical atherosclerosis in healthy, non-medicated middle-aged individuals and to compare these findings with classical cardiovascular risk factors and biochemical markers of inflammation and endothelial dysfunction. Methods: Participants were recruited from the Danish Risk Score Study. A total of 277 individuals, 66% of the invited, aged 50 or 60 years, without known cardiovascular disease or diabetes were randomly selected from national registries. Persons who did not take any medication represented the study population, N = 209. The population was separated in two groups according to coronary artery calcification (CAC) by non-enhanced cardiac-CT, and carotid plaque by ultrasound. Inflammation (C-reactive protein (CRP), fibrinogen and D-dimer) and endothelial dysfunction (von Willebrand factor, tissueplasminogen activator (t-PA), and plasminogen activator inhibitor-1 (PAI-1)) were studied. Results: Coronary calcification was seen in 39 % while carotid plaque was observed in 28 % of individuals. Age, gender, smoking, blood pressure and triglycerides were significantly associated with CAC (P , 0.05), while age and smoking were significantly associated with carotid plaque (P , 0.005).The concentrations of t-PA and PAI-1 were significantly associated with CAC (P , 0.01), whereas fibrinogen levels were associated with plaque (P , 0.05). Logistic multivariate regression revealed that age, gender, systolic blood pressure and total cholesterol (P , 0.1) were associated with CAC, while no associations were found with carotid plaque. Conclusion: Even in apparantly healthy middle-aged individuals manifest morphological and biochemical signs of subclinical atherosclerosis were observed in more than 50 %. Markers of inflammation and endothelial dysfunction were associated with subclinical atherosclerosis in asymptomatic healthy individuals, but these changes seemed to be mediated through classical risk factors. Furthermore, we demonstrate that classical risk factors and biochemical markers are associated differently with atherosclerotic lesions depending on the vascular location.

Research paper thumbnail of Sex difference in clot lysability and association to coronary artery calcification

Biology of Sex Differences, Feb 13, 2018

Background: Incidence and prevalence of cardiovascular disease (CVD) differ between sexes, and wo... more Background: Incidence and prevalence of cardiovascular disease (CVD) differ between sexes, and women experience CVD later than men. Changes in fibrin clot lysability are associated with CVD, and the present study addresses sex differences in fibrin clot lysability in asymptomatic middle-aged individuals and the relation to coronary artery calcification (CAC). Methods: Participants free of morbidities and medication, N = 163, were randomly chosen from a national registry among citizens, 50 or 60 years of age, and were followed for 5 years. CAC was determined by the Agatston (Ag) score both at baseline and at follow-up. Based on the changes in Ag, the population was divided into two groups: ΔAg = 0 U or ΔAg > 0 U. Fibrin clot analyses were based on turbidimetric methods. Results: At baseline, 116 women and 97 men were included; 84 women and 79 men completed the 5-year follow-up (77%). Independently of covariates, women with ΔAg > 0 had reduced mean (SD) fibrin lysability at follow-up, 40.2% (15.9), both in comparison to baseline, 47.8% (20.4), p = 0.001, to women with ΔAg = 0 U, 51. 2% (24.5), p = 0.028, and to men with ΔAg > 0 U, 54.4% (21.0), p = 0.002. Conclusions: Fibrin clot lysability changes over time with considerable sex differences. Women with progression of CAC have reduced fibrin clot lysability compared to men, indicating a sex-specific association between morphological vessel wall changes and fibrin clot lysability.

Research paper thumbnail of Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 5 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Targets down-regulated after 24 h Targets down-regulated after 48 h

Research paper thumbnail of Supplementary Table 3 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Figure 3 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Supplementary Fig. S3. Real time RT-PCR validation of miR-21 transfection efficacy (above) and mi... more Supplementary Fig. S3. Real time RT-PCR validation of miR-21 transfection efficacy (above) and miR-21 target regulation (below). PCR validation of miR-21 regulation 6, 16 and 24 hours following transfection of T24 cells with either a miR-21 precursor or a miR-21 LNA knockdown probe. Expression levels are normalized by comparing to the expression level of miR-21 when transfecting with a scrambled sequence. The fold changes on the y-axis are log2 transformed.

Research paper thumbnail of Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Data from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death <i>In vitro</i&gt

Research paper thumbnail of Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 6 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associat... more Supplementary Figure 1 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associate... more Supplementary Table 4 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Research paper thumbnail of Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro

Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associat... more Supplementary Figure 2 from Genomic Profiling of MicroRNAs in Bladder Cancer: miR-129 Is Associated with Poor Outcome and Promotes Cell Death In vitro