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Papers by Ramutė Mišeikienė
Animals
The aim of this study was to collect samples of bovine colostrum (BCOL) from different sources (a... more The aim of this study was to collect samples of bovine colostrum (BCOL) from different sources (agricultural companies A, B, C, D and E) in Lithuania and to ascertain the influence of lacto-fermentation with Lactiplantibacillus plantarum strain 135 and Lacticaseibacillus paracasei strain 244 on the changes in bovine colostrum amino (AA), biogenic amine (BA), and fatty acid (FA) profiles. It was established that the source of the bovine colostrum, the used LAB, and their interaction had significant effects (p < 0.05) on AA contents; lactic acid bacteria (LAB) used for fermentation was a significant factor for aspartic acid, threonine, glycine, alanine, methionine, phenylalanine, lysine, histidine, and tyrosine; and these factor’s interaction is significant on most of the detected AA concentrations. Total BA content showed significant correlations with glutamic acid, serine, aspartic acid, valine, methionine, phenylalanine, histidine, and gamma amino-butyric acid content in bovine ...
Medycyna Weterynaryjna, 2005
Veterinarija Ir Zootechnika, 2014
Bulgarian Journal of Veterinary Medicine, 2015
The primary objective of premilking udder preparation and teats antiseptic is to achieve an accep... more The primary objective of premilking udder preparation and teats antiseptic is to achieve an acceptable level of decontamination of teat skin. This aids in reducing the spread of microorganisms and incidence of new intramammary infections, and in minimising the number of bacteria. The aim of our studies was to investigate the influence of pre-milking teat antiseptic solutions on total bacterial contamination of teat skin. Three udder antiseptics were applied: Dermisan 0.5% (active ingredientaminopropyl laurylamine), 0.2% solution with active ingredient iodine, and foaming solution of natural compounds (lactic acid+glycerol+allantoin). Cow teats were swabbed before and after application of udder preparations. The total bacterial contamination on cows teat skin was determined employing serial dilutions and plate count method. Microorganisms were identified under uniform conditions on McConkey (for Gram-negative bacteria), Columbia blood agar medium (for staphylococci), containing 5% sheep's blood, Edwards (for streptococci) agars. The statistical analysis of the data was performed using descriptive statistics and independent-sample T test procedures in SPSS 13.0 for Windows. The udder applications with lactic acid and iodine had the highest probability (P<0.05) of reducing total bacterial contamination. The use of udder antiseptics for premilking teats preparation reduced the levels of coliforms, coagulase negative staphylococci and Streptococcus uberis but with exception of iodine, no effect was found on reducing Candida genus yeasts.
Polish Journal of Veterinary Sciences, 2007
The intrabreed and interbreed genetic diversity of Lithuanian cattle breeds - two native, namely ... more The intrabreed and interbreed genetic diversity of Lithuanian cattle breeds - two native, namely Lithuanian Light Grey and Lithuanian White-Backed and two modern, namely Lithuanian Red and Lithuanian Black and White was investigated by determination of genetic markers: 4 milk protein systems, Alpha(s1)-casein, Kappa-casein, Beta-casein and Beta-lactoglobulin, which are comprised of 12 different milk protein types. According to results, the B type of Alpha(s1)-casein was found as predominant in all four studied breeds. The most common A and B types of Kappa-casein were found at high frequency in all investigated cattle breeds. All investigated Lithuanian dairy cattle breeds had high frequency of Beta-lactoglobulin whey protein B types, with the highest frequency in Lithuanian Red breed, and the lowest in Lithuanian Light Grey. After investigation the diversity of alleles and genotypes of milk proteins in Lithuanian dairy cattle breeds was determined that, Lithuanian Red breed was distinguished private C allele and BC genotype of Beta-lactoglobulin and CC genotype of Alpha(s1)-casein. The interbreed genetic diversity was estimated by a principal component analysis (PCA). The first principal component (PC) explains 63.39% and the second principal component (PC) explains 33.67% of the genetics diversity between the breeds. Principal component analysis, suggests the hypothesis that native Lithuanian White Backed and Lithuanian Light Grey breeds still have traits tracing to old native populations.
Journal of Animal and Feed Sciences, Dec 6, 2011
The impact of two tillage systems (conventional and no-tillage) on cereal contamination with fung... more The impact of two tillage systems (conventional and no-tillage) on cereal contamination with fungi and Fusarium mycotoxins was investigated. Samples of soil, cereal seeds, seedlings and freshly harvested grain were taken from plots of an agricultural cooperative in Lithuania. All samples were subjected to mycological analyses. Grain samples were also used for analysis of mycotoxins. In the soil under no-tillage, contamination with fungal spores was 92.9% higher compared to the soil under conventional tillage. In the soil of the no-tillage system we found 20 fungal genera, while in the soil under conventional tillage the number of genera was 15% lower. On cereal seedlings in the no-tillage system fungal spore content was 24.6% higher (P>0.05) and detection frequency of Fusarium fungi was 22.9% higher (P>0.05) than on seedlings in the conventional system. During harvest in feeding grain grown in the conventional tillage system, 2.2-8.0 log 10 CFU g-1 fungal spores were detected, compared to 2.5-12.0 log 10 CFU g-1 in grain in the no-tillage system. A significantly higher grain contamination with Fusarium spp. was detected in the no-tillage system. However, the deoxynivalenol, zearalenone and T-2 toxin content of grain was not considerably influenced by the different tillage systems, although it varied between species.
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT, Apr 19, 2023
Kaunas : Lietuvos sveikatos mokslų universiteto Leidybos namai, 2021
Journal of Applied Animal Research, 2022
Animals
The aim of this study was to collect samples of bovine colostrum (BCOL) from different sources (a... more The aim of this study was to collect samples of bovine colostrum (BCOL) from different sources (agricultural companies A, B, C, D and E) in Lithuania and to ascertain the influence of lacto-fermentation with Lactiplantibacillus plantarum strain 135 and Lacticaseibacillus paracasei strain 244 on the changes in bovine colostrum amino (AA), biogenic amine (BA), and fatty acid (FA) profiles. It was established that the source of the bovine colostrum, the used LAB, and their interaction had significant effects (p < 0.05) on AA contents; lactic acid bacteria (LAB) used for fermentation was a significant factor for aspartic acid, threonine, glycine, alanine, methionine, phenylalanine, lysine, histidine, and tyrosine; and these factor’s interaction is significant on most of the detected AA concentrations. Total BA content showed significant correlations with glutamic acid, serine, aspartic acid, valine, methionine, phenylalanine, histidine, and gamma amino-butyric acid content in bovine ...
Medycyna Weterynaryjna, 2005
Veterinarija Ir Zootechnika, 2014
Bulgarian Journal of Veterinary Medicine, 2015
The primary objective of premilking udder preparation and teats antiseptic is to achieve an accep... more The primary objective of premilking udder preparation and teats antiseptic is to achieve an acceptable level of decontamination of teat skin. This aids in reducing the spread of microorganisms and incidence of new intramammary infections, and in minimising the number of bacteria. The aim of our studies was to investigate the influence of pre-milking teat antiseptic solutions on total bacterial contamination of teat skin. Three udder antiseptics were applied: Dermisan 0.5% (active ingredientaminopropyl laurylamine), 0.2% solution with active ingredient iodine, and foaming solution of natural compounds (lactic acid+glycerol+allantoin). Cow teats were swabbed before and after application of udder preparations. The total bacterial contamination on cows teat skin was determined employing serial dilutions and plate count method. Microorganisms were identified under uniform conditions on McConkey (for Gram-negative bacteria), Columbia blood agar medium (for staphylococci), containing 5% sheep's blood, Edwards (for streptococci) agars. The statistical analysis of the data was performed using descriptive statistics and independent-sample T test procedures in SPSS 13.0 for Windows. The udder applications with lactic acid and iodine had the highest probability (P<0.05) of reducing total bacterial contamination. The use of udder antiseptics for premilking teats preparation reduced the levels of coliforms, coagulase negative staphylococci and Streptococcus uberis but with exception of iodine, no effect was found on reducing Candida genus yeasts.
Polish Journal of Veterinary Sciences, 2007
The intrabreed and interbreed genetic diversity of Lithuanian cattle breeds - two native, namely ... more The intrabreed and interbreed genetic diversity of Lithuanian cattle breeds - two native, namely Lithuanian Light Grey and Lithuanian White-Backed and two modern, namely Lithuanian Red and Lithuanian Black and White was investigated by determination of genetic markers: 4 milk protein systems, Alpha(s1)-casein, Kappa-casein, Beta-casein and Beta-lactoglobulin, which are comprised of 12 different milk protein types. According to results, the B type of Alpha(s1)-casein was found as predominant in all four studied breeds. The most common A and B types of Kappa-casein were found at high frequency in all investigated cattle breeds. All investigated Lithuanian dairy cattle breeds had high frequency of Beta-lactoglobulin whey protein B types, with the highest frequency in Lithuanian Red breed, and the lowest in Lithuanian Light Grey. After investigation the diversity of alleles and genotypes of milk proteins in Lithuanian dairy cattle breeds was determined that, Lithuanian Red breed was distinguished private C allele and BC genotype of Beta-lactoglobulin and CC genotype of Alpha(s1)-casein. The interbreed genetic diversity was estimated by a principal component analysis (PCA). The first principal component (PC) explains 63.39% and the second principal component (PC) explains 33.67% of the genetics diversity between the breeds. Principal component analysis, suggests the hypothesis that native Lithuanian White Backed and Lithuanian Light Grey breeds still have traits tracing to old native populations.
Journal of Animal and Feed Sciences, Dec 6, 2011
The impact of two tillage systems (conventional and no-tillage) on cereal contamination with fung... more The impact of two tillage systems (conventional and no-tillage) on cereal contamination with fungi and Fusarium mycotoxins was investigated. Samples of soil, cereal seeds, seedlings and freshly harvested grain were taken from plots of an agricultural cooperative in Lithuania. All samples were subjected to mycological analyses. Grain samples were also used for analysis of mycotoxins. In the soil under no-tillage, contamination with fungal spores was 92.9% higher compared to the soil under conventional tillage. In the soil of the no-tillage system we found 20 fungal genera, while in the soil under conventional tillage the number of genera was 15% lower. On cereal seedlings in the no-tillage system fungal spore content was 24.6% higher (P>0.05) and detection frequency of Fusarium fungi was 22.9% higher (P>0.05) than on seedlings in the conventional system. During harvest in feeding grain grown in the conventional tillage system, 2.2-8.0 log 10 CFU g-1 fungal spores were detected, compared to 2.5-12.0 log 10 CFU g-1 in grain in the no-tillage system. A significantly higher grain contamination with Fusarium spp. was detected in the no-tillage system. However, the deoxynivalenol, zearalenone and T-2 toxin content of grain was not considerably influenced by the different tillage systems, although it varied between species.
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT, Apr 19, 2023
Kaunas : Lietuvos sveikatos mokslų universiteto Leidybos namai, 2021
Journal of Applied Animal Research, 2022