Rana H.Raheema - Academia.edu (original) (raw)

Papers by Rana H.Raheema

Research paper thumbnail of Effect of Pomegranate Peel Extract on some Biochemical and Histopathological Parameters in Experimental Induced Mice with Staphylococcus aureus

Journal of Animal Health and Production, 2016

This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatin... more This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatinine, serum uric acid and serum total protein in normal and Staphylococcus aureus infected mice. Thirty seven milk samples were collected from Al-Kut city from cows suffering acute mastitis. After biochemical tests and API Staph System it was revealed that 19 out of 37 milk samples contained S. aureus. Ethanolic extract of pomegranate peels was prepared and the highest in vitro antibacterial effect was observed by 300mg/mL against S. aureus with 30.00 ± 0.57 mm of the diameter zone. Thirty lactating mice were divided into three groups. Group (1): negative control (Normal). Group (2): positive control, mice were infected by intramammary injection of S. aureus at 7, 15, 21 and 30 days and left untreated. Group (3): mice infected with intramammary injection of S. aureus and treated orally with 300 mg/kg BW of ethanolic extract of pomegranate peel after 48 h of infection for 15, 21 and 30 days. At the end of experiment, blood samples were taken from anaesthetized mice then the mice were killed for histopathological examination and measured the following: serum glucose, creatinine, serum uric acid and serum total proteins. Results showed significant increase (P <0.05) in the levels of serum glucose, creatinine, serum uric acid and serum total proteins in positive control mice as compared with negative control while pomegranate peel treated group showed a significant (P <0.05) decrease in the levels of serum glucose, creatinine, serum uric acid and serum total proteins. Histological section in mammary gland showed clear pathological effects of S. aureus as compared with those treated with pomegranate peel extracts.

Research paper thumbnail of Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella pneumonia and Enterobacter aerogenes Isolated from patients with Ocular eye infection in Wasit province, Iraq

World Journal of Advanced Research and Reviews

In Iraq and other developing countries, ocular infections are a significant public health concern... more In Iraq and other developing countries, ocular infections are a significant public health concern, particularly gram-negative bacterial infections, which are common. The emergence of multidrug resistance is a critical concern because it can lead to treatment failure when bacterial strains become resistant to antimicrobial drugs. This study aimed to investigate, Phylogenetic tree analysis of Klebsiella pneumonia and Enterobacter aerogenes isolated from patients with Ocular eye infection. The tree provided evidence of a unique species that was represented by a single nucleic acid sequence. This species encompassed both Klebsiella pneumoniae and Enterobacter aerogenes. Through phylogenetic tree analysis, it was determined that the 16SrRNA gene exhibited effective discriminatory power in identifying these bacterial strains. Based on the sequencing data, it can be inferred that there is a significant sequence similarity between the Klebsiella pneumoniae samples and strains from Banglades...

Research paper thumbnail of Assessment of causes, knowledge and risk factors of Parkinson's disease among general population in Wasit Province, Iraq

World Journal of Advanced Research and Reviews

Background: Millions of individuals worldwide are afflicted by the widespread, progressive neurol... more Background: Millions of individuals worldwide are afflicted by the widespread, progressive neurological condition known as Parkinson's disease. This study sought to identify the causes, risk factors, and general public understanding of Parkinson disease in Wasit, Iraq. Methods: This cross-sectional research in Iraq involved 484 people in total. The period of data collection is from December 1, 2021, to April 15, 2022. A face-to-face interview and an electronic platform made public on social media were used to fill out a pre-tested questionnaire. Both descriptive and analytical statistics were used to analyze the data. Result: According to the individuals' sociodemographic characteristics, there were 305 females (63%) and 179 men (37%) among them. 307 respondents, or 63.2%, were from Wasit. The majority of participants are aware that the brain is the organ most commonly impacted, and they also ask additional questions. Conclusion: The patients and medical student may have acc...

Research paper thumbnail of Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

World Journal Of Advanced Research and Reviews, Nov 30, 2022

Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infect... more Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections.. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)Proteus mirabilis, 2 (2.4%) Acinetobacter baumannii , 1 (1.2%) Serratia marcescens and 1 (1.2%) Burkholderia cepacia group bacteria. The results of this study showed that the diagnostic test for 16S rRNA bacteria (Proteus mirabilis), which numbered 5 (100%), bacteria (Acinetobacter baumannii), which numbered 2 (100%), bacteria (Burkholderia cepacia), which numbered 1 (100%), bacteria (Serratia marcescens), which numbered 1 (100%), and Bacteria (klebsiella oxytoca), which numbered 1 (100%) after completing the phenotypic and biochemical diagnosis diagnostic test (16S rRNA).

Research paper thumbnail of Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients in wasit province, Iraqin wasit province, Iraq

World Journal of Biology Pharmacy and Health Sciences, Nov 30, 2022

ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contami... more ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment and devices is a significant cause of nosocomial infections. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. 30(36.5%) were female and 52(63.5%) were male patients admitted to the intensive care unit distributed 69 (84.1%) gram-negative bacteria .while gram positive 13(15.8%), as well as 20 pure isolates of some places, materials and equipment distributed 12 (75%) Gram negative bacteria .While 8 (25%) it is gram positive bacteria. Results of antibiotic susceptibility test only Trimethoprime was effective choice against Acinetobacter baumannii. Phenotypically survey of carbapenem klebsiella pneumoniae were 9(47%), pseudomonas aeruginosa was 7(35% of MBL and Carbapenemase and the result of MBL and Carbapenemase for E. Coli 7(43%) similar results (40%). Whereas, Genotypically the most prevalent gene in E.Coli 1 (10%), the result was for Burkholderia cepacia blaVIM 1(100%), its prevalence was 7 (53%) among bacteria Klebsiella pneumonia for NDM, the result of bacteria Pseudomonas aeruginosa for this gene was 4(33.3%) and Escherichia Coli was 1(10%), its prevalence was (100%) among klebsiella oxytoca for KPC.

Research paper thumbnail of World Sight Day 2021

Research paper thumbnail of Virulence factors

Research paper thumbnail of دليل الترقيات العلمية حسب التعليمات رقم 167 لعام 2017

Research paper thumbnail of Francisella Contents

Research paper thumbnail of Actinomycetes

Research paper thumbnail of Pre-eclampsia 2019

Research paper thumbnail of Plasmid

Research paper thumbnail of Biofilm

Research paper thumbnail of Eczema

Research paper thumbnail of Gardnerella Vaginitis

Research paper thumbnail of COVID-19 immunity and pathogenicity

Research paper thumbnail of Gene therapy

Research paper thumbnail of Auto immune disease

Research paper thumbnail of Happy Mother's Day

Research paper thumbnail of Sepsis

Research paper thumbnail of Effect of Pomegranate Peel Extract on some Biochemical and Histopathological Parameters in Experimental Induced Mice with Staphylococcus aureus

Journal of Animal Health and Production, 2016

This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatin... more This study was aimed to evaluate the effect of pomegranate peel extract on serum glucose, creatinine, serum uric acid and serum total protein in normal and Staphylococcus aureus infected mice. Thirty seven milk samples were collected from Al-Kut city from cows suffering acute mastitis. After biochemical tests and API Staph System it was revealed that 19 out of 37 milk samples contained S. aureus. Ethanolic extract of pomegranate peels was prepared and the highest in vitro antibacterial effect was observed by 300mg/mL against S. aureus with 30.00 ± 0.57 mm of the diameter zone. Thirty lactating mice were divided into three groups. Group (1): negative control (Normal). Group (2): positive control, mice were infected by intramammary injection of S. aureus at 7, 15, 21 and 30 days and left untreated. Group (3): mice infected with intramammary injection of S. aureus and treated orally with 300 mg/kg BW of ethanolic extract of pomegranate peel after 48 h of infection for 15, 21 and 30 days. At the end of experiment, blood samples were taken from anaesthetized mice then the mice were killed for histopathological examination and measured the following: serum glucose, creatinine, serum uric acid and serum total proteins. Results showed significant increase (P <0.05) in the levels of serum glucose, creatinine, serum uric acid and serum total proteins in positive control mice as compared with negative control while pomegranate peel treated group showed a significant (P <0.05) decrease in the levels of serum glucose, creatinine, serum uric acid and serum total proteins. Histological section in mammary gland showed clear pathological effects of S. aureus as compared with those treated with pomegranate peel extracts.

Research paper thumbnail of Phylogenetic tree analysis based on the 16S sequence alignment for Klebsiella pneumonia and Enterobacter aerogenes Isolated from patients with Ocular eye infection in Wasit province, Iraq

World Journal of Advanced Research and Reviews

In Iraq and other developing countries, ocular infections are a significant public health concern... more In Iraq and other developing countries, ocular infections are a significant public health concern, particularly gram-negative bacterial infections, which are common. The emergence of multidrug resistance is a critical concern because it can lead to treatment failure when bacterial strains become resistant to antimicrobial drugs. This study aimed to investigate, Phylogenetic tree analysis of Klebsiella pneumonia and Enterobacter aerogenes isolated from patients with Ocular eye infection. The tree provided evidence of a unique species that was represented by a single nucleic acid sequence. This species encompassed both Klebsiella pneumoniae and Enterobacter aerogenes. Through phylogenetic tree analysis, it was determined that the 16SrRNA gene exhibited effective discriminatory power in identifying these bacterial strains. Based on the sequencing data, it can be inferred that there is a significant sequence similarity between the Klebsiella pneumoniae samples and strains from Banglades...

Research paper thumbnail of Assessment of causes, knowledge and risk factors of Parkinson's disease among general population in Wasit Province, Iraq

World Journal of Advanced Research and Reviews

Background: Millions of individuals worldwide are afflicted by the widespread, progressive neurol... more Background: Millions of individuals worldwide are afflicted by the widespread, progressive neurological condition known as Parkinson's disease. This study sought to identify the causes, risk factors, and general public understanding of Parkinson disease in Wasit, Iraq. Methods: This cross-sectional research in Iraq involved 484 people in total. The period of data collection is from December 1, 2021, to April 15, 2022. A face-to-face interview and an electronic platform made public on social media were used to fill out a pre-tested questionnaire. Both descriptive and analytical statistics were used to analyze the data. Result: According to the individuals' sociodemographic characteristics, there were 305 females (63%) and 179 men (37%) among them. 307 respondents, or 63.2%, were from Wasit. The majority of participants are aware that the brain is the organ most commonly impacted, and they also ask additional questions. Conclusion: The patients and medical student may have acc...

Research paper thumbnail of Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

World Journal Of Advanced Research and Reviews, Nov 30, 2022

Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infect... more Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections.. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)Proteus mirabilis, 2 (2.4%) Acinetobacter baumannii , 1 (1.2%) Serratia marcescens and 1 (1.2%) Burkholderia cepacia group bacteria. The results of this study showed that the diagnostic test for 16S rRNA bacteria (Proteus mirabilis), which numbered 5 (100%), bacteria (Acinetobacter baumannii), which numbered 2 (100%), bacteria (Burkholderia cepacia), which numbered 1 (100%), bacteria (Serratia marcescens), which numbered 1 (100%), and Bacteria (klebsiella oxytoca), which numbered 1 (100%) after completing the phenotypic and biochemical diagnosis diagnostic test (16S rRNA).

Research paper thumbnail of Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients in wasit province, Iraqin wasit province, Iraq

World Journal of Biology Pharmacy and Health Sciences, Nov 30, 2022

ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contami... more ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment and devices is a significant cause of nosocomial infections. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. 30(36.5%) were female and 52(63.5%) were male patients admitted to the intensive care unit distributed 69 (84.1%) gram-negative bacteria .while gram positive 13(15.8%), as well as 20 pure isolates of some places, materials and equipment distributed 12 (75%) Gram negative bacteria .While 8 (25%) it is gram positive bacteria. Results of antibiotic susceptibility test only Trimethoprime was effective choice against Acinetobacter baumannii. Phenotypically survey of carbapenem klebsiella pneumoniae were 9(47%), pseudomonas aeruginosa was 7(35% of MBL and Carbapenemase and the result of MBL and Carbapenemase for E. Coli 7(43%) similar results (40%). Whereas, Genotypically the most prevalent gene in E.Coli 1 (10%), the result was for Burkholderia cepacia blaVIM 1(100%), its prevalence was 7 (53%) among bacteria Klebsiella pneumonia for NDM, the result of bacteria Pseudomonas aeruginosa for this gene was 4(33.3%) and Escherichia Coli was 1(10%), its prevalence was (100%) among klebsiella oxytoca for KPC.

Research paper thumbnail of World Sight Day 2021

Research paper thumbnail of Virulence factors

Research paper thumbnail of دليل الترقيات العلمية حسب التعليمات رقم 167 لعام 2017

Research paper thumbnail of Francisella Contents

Research paper thumbnail of Actinomycetes

Research paper thumbnail of Pre-eclampsia 2019

Research paper thumbnail of Plasmid

Research paper thumbnail of Biofilm

Research paper thumbnail of Eczema

Research paper thumbnail of Gardnerella Vaginitis

Research paper thumbnail of COVID-19 immunity and pathogenicity

Research paper thumbnail of Gene therapy

Research paper thumbnail of Auto immune disease

Research paper thumbnail of Happy Mother's Day

Research paper thumbnail of Sepsis