Rania Ewida - Academia.edu (original) (raw)
Papers by Rania Ewida
New Valley Veterinary Journal (Online), 2023
Gut Pathogens
Background Non-typhoidal Salmonella (NTS) is a major foodborne zoonotic pathogen worldwide. In th... more Background Non-typhoidal Salmonella (NTS) is a major foodborne zoonotic pathogen worldwide. In the current study, Various NTS strains were isolated from (cows, milk and dairy products in addition to humans) in New Valley and Assiut Governorate, Egypt. NTS were firstly serotyped and tested by antibiotic sensitivity test. Secondly, some virulence genes and Antibiotic resistance genes have been identified by using PCR. Finally, Phylogenesis was performed depending on the invA gene, for two S. typhimurium isolates (one of animal origin and the other of human origin for evaluating zoonotic potential). Results Out of 800 examined samples, the total number of isolates was 87 (10.88%), which were classified into 13 serotypes, with the most prevalent being S. Typhimurium and S. enteritidis. Both bovine and human isolates showed the highest resistance to clindamycin and streptomycin, with 90.80% of the tested isolates exhibiting MDR. The occurrence of the invA gene was 100%, while 72.22%, 30....
BMC Veterinary Research
Background Helicobacter pylori is one of the most common bacterial infections and is widespread g... more Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR). Results The prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant as...
Foods
Listeria monocytogenes is one of the most important emerging foodborne pathogens. The objectives ... more Listeria monocytogenes is one of the most important emerging foodborne pathogens. The objectives of this work were to investigate the incidence of Listeria spp. and L. monocytogenes in soft cheese and ice cream in Assiut city, Egypt, and to examine the effect of some probiotic Bifidobacterium spp. (Bifidobacterium breve, Bifidobacterium animalis, or a mixture of the two) on the viability of L. monocytogenes in soft cheese. The existence of Listeria spp. and L. monocytogenes was examined in 30 samples of soft cheese and 30 samples of ice cream. Bacteriological analyses and molecular identification (using 16S rRNA gene and hlyA gene for Listeria spp. and L. monocytogenes, respectively) were performed on those samples. Additionally, Bifidobacterium spp. were incorporated in the making of soft cheese to study their inhibitory impacts on L. monocytogenes. Out of 60 samples of soft cheese and ice cream, 25 samples showed Listeria spp., while L. monocytogenes was found in only 2 soft chees...
Egyptian journal of Immunology
COVID-19 pandemic is a substantial challenge for healthcare systems. Severe acute respiratory syn... more COVID-19 pandemic is a substantial challenge for healthcare systems. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) reverse transcription-polymerase chain reaction (RT-PCR) tests are considered the gold standard technique for diagnosis of symptomatic and asymptomatic infectious viral carriers and for screening special or at-risk populations. The pooled testing procedure is commonly used to reduce the cost of screening a large number of individuals for infectious diseases. This work was conducted to verify the accuracy of the standard SARS COV-2 RT- real-time PCR kit for detecting a single positive sample in a pool of negative samples. Kit verification using negative and positive samples was performed for the selection of the target pool sizes. RNA extracts from 443 healthcare workers, after 15 days’ rotation in EL-Raghy Isolation COVID-19 Hospital, Assiut University during the first outbreak of COVID-19 pandemic (the period from June to September 2020) were obtained and ...
Egyptian Journal of Food Safety, 2018
DOAJ (DOAJ: Directory of Open Access Journals), Oct 3, 2021
Journal of Advanced Veterinary Research, 2018
This study was undertaken to detect the enteropathogenic and shiga toxin-producing Escherichia co... more This study was undertaken to detect the enteropathogenic and shiga toxin-producing Escherichia coli (EPEC and STEC) in 120 milk products samples (soft cheese, hard cheese, yoghurt and ice cream). All samples were submitted for bacteriological examination, serological and molecular identification of virulent and antibiotic resistant genes using eaeA, hylA, blaTEM, blaCTX-M1, blaOXA and blaSHV primers. The bacteriological examination revealed that the incidence of occurrence of EPEC was 3.33% in ice cream samples, while it could not isolated from other types of milk products. In addition, STEC failed to detect in all examined milk products. The isolated EPEC strain following E. coli O18 serotyping. Moreover, the molecular identification of the isolated strain revealed that the strain contains eaeA, blaTEM and blaCTX-M1 genes.
Journal of Advanced Veterinary Research, 2018
Cronobacter sakazakii is one of emerging foodborne pathogens around the world. A total of 90 dair... more Cronobacter sakazakii is one of emerging foodborne pathogens around the world. A total of 90 dairy-based desserts samples (ice cream, Muhallabia and rice pudding) were examined for detecting C. sakazakii. All samples were submitted for bacteriological examination and confirmed by molecular identification using 16S rRNA gene for C. sakazakii. The bacteriological and molecular examination revealed that the incidence of occurrence of C. sakazakii was 5.55% from the total dairy-based desserts samples, the highest percentage occurred in rice pudding samples (10%), while the incidence of C. sakazakii in ice cream and Muhallabia were 3.33% for each type. The results pointed out that high risk for human may occur by contaminated dairy-based desserts. The hygienic precautions must be taken during the processing of these types of products.
Veterinary World, 2020
Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock p... more Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock production with subsequent considerable economic losses. This study was conducted to diagnose Anaplasma and Babesia infection using molecular techniques in imported Aberdeen Angus cattle imported from Uruguay to El-Kharga Oasis in New Valley, Egypt, and to investigate the effects of disease on some serum biochemical and oxidative stress parameters. Materials and Methods: Blood samples were collected from 31 cattle, 21 diseased and ten apparently normal, of varying ages and sex. The blood was used for the preparation of blood smears, polymerase chain reaction assay, and separation of serum for biochemical investigation. The experimental production farm at the Faculty of Agriculture, New Valley University, was infested with ticks and variable clinical manifestations during the period from December 2017 to March 2018. One calf died of a suspected blood parasite infection. Results: The blood ...
June-2020, 2020
Background and Aim: Milk production is one of the main props for the national economy. One of the... more Background and Aim: Milk production is one of the main props for the national economy. One of the crucial problems in this industry is subclinical mastitis, which harms this industry that considered the backbone of the economy. It is an infectious and zoonotic disease; the infection can spread between dairy animals through milkers' hands, and milking machines, while the human infection occurs due to the consumption of apparently hygienic milk. Staphylococcus aureus is one of the main causative agents of clinical and subclinical mastitis. It is also considered one of the bacteria incriminated in food intoxication of humans due to its virulence factors as enterotoxins and toxic shock syndrome. The current study was designed to assess the prevalence of S. aureus and its enterotoxins, as well as, its other virulence factors in milk collected from cows that suffer from subclinical mastitis. Materials and Methods: Sixty cows were collected from different dairy farms located in Assiut ...
Veterinary World, 2018
Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public heal... more Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk sold in Assiut City, Egypt. Materials and Methods: A total of 50 raw buffalo's milk samples were collected and examined for adulteration by addition of cow's milk. The examination carried out by applying polymerase chain reaction-restriction fragment length polymorphism technique using cytochrome b (cyt b) gene primers and Hinf I enzymes. The size of target gene was 360 bp in both animal species and amplicon can be digested using Hinf I enzyme, this restriction enzyme divided the essential band to clear three bands at 360, 210, and 150 bp in cows' milk, while, the enzyme could not be cleaved the amplicon in buffalo's samples. Results: The obtained results cleared that the incidence of adulteration of buffalo's milk very high percentage reaches 90%. Conclusion: It could be concluded that the raw buffalo's milk sold in Assiut City subject to fraudulent practice and thus can lead to public health hazards.
Veterinary World, 2018
Background and Aim: Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal ... more Background and Aim: Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal and human health. Ingestion of contaminated raw milk has been suggested as a vehicle for transmission of Toxoplasma gondii to human. The present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques (enzyme-linked immunosorbent assay [ELISA] and quantitative polymerase chain reaction [qPCR]). Materials and Methods: This study was conducted to determine the T. gondii prevalence using ELISA and qPCR in raw goat, sheep, and camels milk (30 samples for each) collected from different locations in Upper Egypt. Results: T. gondii IgG antibodies were detected in 90.0, 60.0, and 3.33% of goat, sheep, and camel milk samples, respectively. From the positive samples of T. gondii IgG, the parasitic DNA was detected only in two examined milk samples, one of them was present in goat milk sample and another one was found in sheep milk sample. On the other hand, the parasite was not detected in camels' milk samples. Conclusion: These results concluded that the raw milk was contaminated by T. gondii tachyzoites which could be a source of human infection. Restricted hygienic programs should be implemented in the animal farms to decrease the risk of milk contamination by this parasite.
This study aimed to evaluate kareish cheese sold in two cities in Egypt (New Valley and Cairo). A... more This study aimed to evaluate kareish cheese sold in two cities in Egypt (New Valley and Cairo). A total of 105 kareish cheese samples were collected from New Valley, Cairo and different cheese factories (35 samples for each). The samples were examined for the occurrence of some food poisoning microorganisms as well as their quality. All samples were submitted for physical, chemical, and microbiological examination. The physical examination revealed that 100% of the examined samples were normal in color, odor, and consistency. The mean values of salinity and pH were 2.0±0.26 and 4.4±0.04, respectively. The positive % of coliforms, E. coli, and molds & yeasts were 42.8, 30.5, and 89.5%, respectively with the mean count 3.45±0.22, 1.92±0.22, and 4.62±0.13 cfu/g, respectively. Moreover, Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes could not be detected in the examined kareish cheese samples. The results clarified that the high risk for humans that may be occurred by...
New Valley Veterinary Journal (Online), 2022
A hundred fifty randomly collected samples of ice cream, rice pudding and mahlabia (50 samples ea... more A hundred fifty randomly collected samples of ice cream, rice pudding and mahlabia (50 samples each) from different localities in El-Dakhla city, New Valley Governorate, Egypt, were microbiologically examined. The average counts of the aerobic plate, psychrotrophic bacteria, total coliforms, fecal coliforms, Staphylococcus aureus counts and yeast and mold in the examined ice-cream samples were 6
New Valley Veterinary Journal
Staphylococcus aureus (S. aureus) is one of the most important microorganisms causing foodborne o... more Staphylococcus aureus (S. aureus) is one of the most important microorganisms causing foodborne outbreaks worldwide. the present work was planned to study the occurrence, count, and identification of S. aureus and investigation of the obtained isolates for the presence of enterotoxin A encoding gene (sea) in milk and some milk products in New Valley governorate, Egypt. A total of 150 random samples of marketable milk and some milk products including soft cheese (Kareish, Domiati) & ice cream (30 for each) were collected from different localities in the New Valley governorate. S. aureus occurred in 11.6, 10, 3.3 and10% for marketable milk, Kariesh cheese, Domiati cheese, and ice cream, respectively. The presence of the enterotoxin sea gene was identified in 4 (57%) and 2 (50%) in milk and soft cheese, respectively, while none of the isolates obtained from ice cream samples harbored this gene. The investigation revealed that the examined milk samples and its products were contaminated with S. aureus with average counts of 2.8×10 8 , 7.5×10 5 , 7.6×10 6, and 1.7×10 7 for milk, Kariesh cheese, Domiati cheese, and ice cream samples, respectively. In conclusion, the presence of S. aureus in milk and some milk products indicated that the examined samples were of low quality which reflects unhygienic measures during milking, processing, storage, and distribution chain.
New Valley Veterinary Journal (Online), 2023
Gut Pathogens
Background Non-typhoidal Salmonella (NTS) is a major foodborne zoonotic pathogen worldwide. In th... more Background Non-typhoidal Salmonella (NTS) is a major foodborne zoonotic pathogen worldwide. In the current study, Various NTS strains were isolated from (cows, milk and dairy products in addition to humans) in New Valley and Assiut Governorate, Egypt. NTS were firstly serotyped and tested by antibiotic sensitivity test. Secondly, some virulence genes and Antibiotic resistance genes have been identified by using PCR. Finally, Phylogenesis was performed depending on the invA gene, for two S. typhimurium isolates (one of animal origin and the other of human origin for evaluating zoonotic potential). Results Out of 800 examined samples, the total number of isolates was 87 (10.88%), which were classified into 13 serotypes, with the most prevalent being S. Typhimurium and S. enteritidis. Both bovine and human isolates showed the highest resistance to clindamycin and streptomycin, with 90.80% of the tested isolates exhibiting MDR. The occurrence of the invA gene was 100%, while 72.22%, 30....
BMC Veterinary Research
Background Helicobacter pylori is one of the most common bacterial infections and is widespread g... more Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR). Results The prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant as...
Foods
Listeria monocytogenes is one of the most important emerging foodborne pathogens. The objectives ... more Listeria monocytogenes is one of the most important emerging foodborne pathogens. The objectives of this work were to investigate the incidence of Listeria spp. and L. monocytogenes in soft cheese and ice cream in Assiut city, Egypt, and to examine the effect of some probiotic Bifidobacterium spp. (Bifidobacterium breve, Bifidobacterium animalis, or a mixture of the two) on the viability of L. monocytogenes in soft cheese. The existence of Listeria spp. and L. monocytogenes was examined in 30 samples of soft cheese and 30 samples of ice cream. Bacteriological analyses and molecular identification (using 16S rRNA gene and hlyA gene for Listeria spp. and L. monocytogenes, respectively) were performed on those samples. Additionally, Bifidobacterium spp. were incorporated in the making of soft cheese to study their inhibitory impacts on L. monocytogenes. Out of 60 samples of soft cheese and ice cream, 25 samples showed Listeria spp., while L. monocytogenes was found in only 2 soft chees...
Egyptian journal of Immunology
COVID-19 pandemic is a substantial challenge for healthcare systems. Severe acute respiratory syn... more COVID-19 pandemic is a substantial challenge for healthcare systems. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) reverse transcription-polymerase chain reaction (RT-PCR) tests are considered the gold standard technique for diagnosis of symptomatic and asymptomatic infectious viral carriers and for screening special or at-risk populations. The pooled testing procedure is commonly used to reduce the cost of screening a large number of individuals for infectious diseases. This work was conducted to verify the accuracy of the standard SARS COV-2 RT- real-time PCR kit for detecting a single positive sample in a pool of negative samples. Kit verification using negative and positive samples was performed for the selection of the target pool sizes. RNA extracts from 443 healthcare workers, after 15 days’ rotation in EL-Raghy Isolation COVID-19 Hospital, Assiut University during the first outbreak of COVID-19 pandemic (the period from June to September 2020) were obtained and ...
Egyptian Journal of Food Safety, 2018
DOAJ (DOAJ: Directory of Open Access Journals), Oct 3, 2021
Journal of Advanced Veterinary Research, 2018
This study was undertaken to detect the enteropathogenic and shiga toxin-producing Escherichia co... more This study was undertaken to detect the enteropathogenic and shiga toxin-producing Escherichia coli (EPEC and STEC) in 120 milk products samples (soft cheese, hard cheese, yoghurt and ice cream). All samples were submitted for bacteriological examination, serological and molecular identification of virulent and antibiotic resistant genes using eaeA, hylA, blaTEM, blaCTX-M1, blaOXA and blaSHV primers. The bacteriological examination revealed that the incidence of occurrence of EPEC was 3.33% in ice cream samples, while it could not isolated from other types of milk products. In addition, STEC failed to detect in all examined milk products. The isolated EPEC strain following E. coli O18 serotyping. Moreover, the molecular identification of the isolated strain revealed that the strain contains eaeA, blaTEM and blaCTX-M1 genes.
Journal of Advanced Veterinary Research, 2018
Cronobacter sakazakii is one of emerging foodborne pathogens around the world. A total of 90 dair... more Cronobacter sakazakii is one of emerging foodborne pathogens around the world. A total of 90 dairy-based desserts samples (ice cream, Muhallabia and rice pudding) were examined for detecting C. sakazakii. All samples were submitted for bacteriological examination and confirmed by molecular identification using 16S rRNA gene for C. sakazakii. The bacteriological and molecular examination revealed that the incidence of occurrence of C. sakazakii was 5.55% from the total dairy-based desserts samples, the highest percentage occurred in rice pudding samples (10%), while the incidence of C. sakazakii in ice cream and Muhallabia were 3.33% for each type. The results pointed out that high risk for human may occur by contaminated dairy-based desserts. The hygienic precautions must be taken during the processing of these types of products.
Veterinary World, 2020
Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock p... more Background and Aim: Anaplasmosis and babesiosis are tick-borne diseases that threaten livestock production with subsequent considerable economic losses. This study was conducted to diagnose Anaplasma and Babesia infection using molecular techniques in imported Aberdeen Angus cattle imported from Uruguay to El-Kharga Oasis in New Valley, Egypt, and to investigate the effects of disease on some serum biochemical and oxidative stress parameters. Materials and Methods: Blood samples were collected from 31 cattle, 21 diseased and ten apparently normal, of varying ages and sex. The blood was used for the preparation of blood smears, polymerase chain reaction assay, and separation of serum for biochemical investigation. The experimental production farm at the Faculty of Agriculture, New Valley University, was infested with ticks and variable clinical manifestations during the period from December 2017 to March 2018. One calf died of a suspected blood parasite infection. Results: The blood ...
June-2020, 2020
Background and Aim: Milk production is one of the main props for the national economy. One of the... more Background and Aim: Milk production is one of the main props for the national economy. One of the crucial problems in this industry is subclinical mastitis, which harms this industry that considered the backbone of the economy. It is an infectious and zoonotic disease; the infection can spread between dairy animals through milkers' hands, and milking machines, while the human infection occurs due to the consumption of apparently hygienic milk. Staphylococcus aureus is one of the main causative agents of clinical and subclinical mastitis. It is also considered one of the bacteria incriminated in food intoxication of humans due to its virulence factors as enterotoxins and toxic shock syndrome. The current study was designed to assess the prevalence of S. aureus and its enterotoxins, as well as, its other virulence factors in milk collected from cows that suffer from subclinical mastitis. Materials and Methods: Sixty cows were collected from different dairy farms located in Assiut ...
Veterinary World, 2018
Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public heal... more Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk sold in Assiut City, Egypt. Materials and Methods: A total of 50 raw buffalo's milk samples were collected and examined for adulteration by addition of cow's milk. The examination carried out by applying polymerase chain reaction-restriction fragment length polymorphism technique using cytochrome b (cyt b) gene primers and Hinf I enzymes. The size of target gene was 360 bp in both animal species and amplicon can be digested using Hinf I enzyme, this restriction enzyme divided the essential band to clear three bands at 360, 210, and 150 bp in cows' milk, while, the enzyme could not be cleaved the amplicon in buffalo's samples. Results: The obtained results cleared that the incidence of adulteration of buffalo's milk very high percentage reaches 90%. Conclusion: It could be concluded that the raw buffalo's milk sold in Assiut City subject to fraudulent practice and thus can lead to public health hazards.
Veterinary World, 2018
Background and Aim: Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal ... more Background and Aim: Toxoplasmosis is a worldwide zoonotic disease with harmful effects on animal and human health. Ingestion of contaminated raw milk has been suggested as a vehicle for transmission of Toxoplasma gondii to human. The present study was performed for the detection of T. gondii in raw milk samples of goat, sheep, and camel in Upper Egypt using two different techniques (enzyme-linked immunosorbent assay [ELISA] and quantitative polymerase chain reaction [qPCR]). Materials and Methods: This study was conducted to determine the T. gondii prevalence using ELISA and qPCR in raw goat, sheep, and camels milk (30 samples for each) collected from different locations in Upper Egypt. Results: T. gondii IgG antibodies were detected in 90.0, 60.0, and 3.33% of goat, sheep, and camel milk samples, respectively. From the positive samples of T. gondii IgG, the parasitic DNA was detected only in two examined milk samples, one of them was present in goat milk sample and another one was found in sheep milk sample. On the other hand, the parasite was not detected in camels' milk samples. Conclusion: These results concluded that the raw milk was contaminated by T. gondii tachyzoites which could be a source of human infection. Restricted hygienic programs should be implemented in the animal farms to decrease the risk of milk contamination by this parasite.
This study aimed to evaluate kareish cheese sold in two cities in Egypt (New Valley and Cairo). A... more This study aimed to evaluate kareish cheese sold in two cities in Egypt (New Valley and Cairo). A total of 105 kareish cheese samples were collected from New Valley, Cairo and different cheese factories (35 samples for each). The samples were examined for the occurrence of some food poisoning microorganisms as well as their quality. All samples were submitted for physical, chemical, and microbiological examination. The physical examination revealed that 100% of the examined samples were normal in color, odor, and consistency. The mean values of salinity and pH were 2.0±0.26 and 4.4±0.04, respectively. The positive % of coliforms, E. coli, and molds & yeasts were 42.8, 30.5, and 89.5%, respectively with the mean count 3.45±0.22, 1.92±0.22, and 4.62±0.13 cfu/g, respectively. Moreover, Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes could not be detected in the examined kareish cheese samples. The results clarified that the high risk for humans that may be occurred by...
New Valley Veterinary Journal (Online), 2022
A hundred fifty randomly collected samples of ice cream, rice pudding and mahlabia (50 samples ea... more A hundred fifty randomly collected samples of ice cream, rice pudding and mahlabia (50 samples each) from different localities in El-Dakhla city, New Valley Governorate, Egypt, were microbiologically examined. The average counts of the aerobic plate, psychrotrophic bacteria, total coliforms, fecal coliforms, Staphylococcus aureus counts and yeast and mold in the examined ice-cream samples were 6
New Valley Veterinary Journal
Staphylococcus aureus (S. aureus) is one of the most important microorganisms causing foodborne o... more Staphylococcus aureus (S. aureus) is one of the most important microorganisms causing foodborne outbreaks worldwide. the present work was planned to study the occurrence, count, and identification of S. aureus and investigation of the obtained isolates for the presence of enterotoxin A encoding gene (sea) in milk and some milk products in New Valley governorate, Egypt. A total of 150 random samples of marketable milk and some milk products including soft cheese (Kareish, Domiati) & ice cream (30 for each) were collected from different localities in the New Valley governorate. S. aureus occurred in 11.6, 10, 3.3 and10% for marketable milk, Kariesh cheese, Domiati cheese, and ice cream, respectively. The presence of the enterotoxin sea gene was identified in 4 (57%) and 2 (50%) in milk and soft cheese, respectively, while none of the isolates obtained from ice cream samples harbored this gene. The investigation revealed that the examined milk samples and its products were contaminated with S. aureus with average counts of 2.8×10 8 , 7.5×10 5 , 7.6×10 6, and 1.7×10 7 for milk, Kariesh cheese, Domiati cheese, and ice cream samples, respectively. In conclusion, the presence of S. aureus in milk and some milk products indicated that the examined samples were of low quality which reflects unhygienic measures during milking, processing, storage, and distribution chain.