Rashmi Kumar - Academia.edu (original) (raw)

Rashmi Kumar

Uploads

Papers by Rashmi Kumar

Research paper thumbnail of Different population histories of the Mundari- and Mon-Khmer-speaking Austro-Asiatic tribes inferred from the mtDNA 9-bp deletion/insertion polymorphism in Indian populations

Research paper thumbnail of Role of oligometric TCR complexes and TCR-interacting molecules in T cell develoment and activation

Abbreviations TCR T cell antigen receptor tet Tetracycline T H cell Helper T cell TM Transmembran... more Abbreviations TCR T cell antigen receptor tet Tetracycline T H cell Helper T cell TM Transmembrane WT Wild Type WB Western blot ZAP70 Zeta-associated protein of 70 kD containing one immunoreceptor tyrosine-based activation motif (ITAM)(Reth, 1989). The cytoplasmic domain of CD3ε contains in addition two more recently identified regions, showing the coexistence of monomeric and oligomeric TCR complexes. The coexistence of TCR complexes of different sizes is supported by the analysis via electron microscopy of the cell surface replicas of resting primary T cells and T cell lines labelled 1.2 TCR oligomers in resting T cells 5. Differential signaling through TCR-CD3 complex leading to alternate fates of DP thymocytes 5.1. Bcl-2 family member proteins subcellular compartmentalization of RasGRP/Ras/Erk to the Golgi membrane, where as negative selection inducing signals led to localization of Grb2-Sos/Ras/Erk to the plasma membrane. (Daniels et al., 2006). 5.2. Ras 5.3. MAPK phosphorylation of GRK2 at Ser685 by PKA (Cong et al., 2001b). As described previously, GRKs play a key role in GPCR signaling and 6.4. Subcellular localization and regulation of GRK2 activity Bibliography

Research paper thumbnail of Antibody repertoire diversification through VH gene replacement in mice cloned from an IgA plasma cell

Proceedings of the National Academy of Sciences of the United States of America, Jan 3, 2015

In mammals, VDJ recombination is responsible for the establishment of a highly diversified preimm... more In mammals, VDJ recombination is responsible for the establishment of a highly diversified preimmune antibody repertoire. Acquisition of a functional Ig heavy (H) chain variable (V) gene rearrangement is thought to prevent further recombination at the IgH locus. Here, we describe VHQ52(NT); Vκgr32(NT) Ig monoclonal mice reprogrammed from the nucleus of an intestinal IgA(+) plasma cell. In VHQ52(NT) mice, IgA replaced IgM to drive early B-cell development and peripheral B-cell maturation. In VHQ52(NT) animals, over 20% of mature B cells disrupted the single productive, nonautoimmune IgH rearrangement through VH replacement and exchanged it with a highly diversified pool of IgH specificities. VH replacement occurred in early pro-B cells, was independent of pre-B-cell receptor signaling, and involved predominantly one adjacent VH germ-line gene. VH replacement was also identified in 5% of peripheral B cells of mice inheriting a different productive VH rearrangement expressed in the for...

Research paper thumbnail of Different population histories of the Mundari- and Mon-Khmer-speaking Austro-Asiatic tribes inferred from the mtDNA 9-bp deletion/insertion polymorphism in Indian populations

Research paper thumbnail of Role of oligometric TCR complexes and TCR-interacting molecules in T cell develoment and activation

Abbreviations TCR T cell antigen receptor tet Tetracycline T H cell Helper T cell TM Transmembran... more Abbreviations TCR T cell antigen receptor tet Tetracycline T H cell Helper T cell TM Transmembrane WT Wild Type WB Western blot ZAP70 Zeta-associated protein of 70 kD containing one immunoreceptor tyrosine-based activation motif (ITAM)(Reth, 1989). The cytoplasmic domain of CD3ε contains in addition two more recently identified regions, showing the coexistence of monomeric and oligomeric TCR complexes. The coexistence of TCR complexes of different sizes is supported by the analysis via electron microscopy of the cell surface replicas of resting primary T cells and T cell lines labelled 1.2 TCR oligomers in resting T cells 5. Differential signaling through TCR-CD3 complex leading to alternate fates of DP thymocytes 5.1. Bcl-2 family member proteins subcellular compartmentalization of RasGRP/Ras/Erk to the Golgi membrane, where as negative selection inducing signals led to localization of Grb2-Sos/Ras/Erk to the plasma membrane. (Daniels et al., 2006). 5.2. Ras 5.3. MAPK phosphorylation of GRK2 at Ser685 by PKA (Cong et al., 2001b). As described previously, GRKs play a key role in GPCR signaling and 6.4. Subcellular localization and regulation of GRK2 activity Bibliography

Research paper thumbnail of Antibody repertoire diversification through VH gene replacement in mice cloned from an IgA plasma cell

Proceedings of the National Academy of Sciences of the United States of America, Jan 3, 2015

In mammals, VDJ recombination is responsible for the establishment of a highly diversified preimm... more In mammals, VDJ recombination is responsible for the establishment of a highly diversified preimmune antibody repertoire. Acquisition of a functional Ig heavy (H) chain variable (V) gene rearrangement is thought to prevent further recombination at the IgH locus. Here, we describe VHQ52(NT); Vκgr32(NT) Ig monoclonal mice reprogrammed from the nucleus of an intestinal IgA(+) plasma cell. In VHQ52(NT) mice, IgA replaced IgM to drive early B-cell development and peripheral B-cell maturation. In VHQ52(NT) animals, over 20% of mature B cells disrupted the single productive, nonautoimmune IgH rearrangement through VH replacement and exchanged it with a highly diversified pool of IgH specificities. VH replacement occurred in early pro-B cells, was independent of pre-B-cell receptor signaling, and involved predominantly one adjacent VH germ-line gene. VH replacement was also identified in 5% of peripheral B cells of mice inheriting a different productive VH rearrangement expressed in the for...

Log In