Piyanee Ratanachamnong - Academia.edu (original) (raw)

Papers by Piyanee Ratanachamnong

Heliyon

Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and ... more Citrus hystrix or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine for health benefits including oral and gum health, stress relief, hair care, and skincare. In this study, we demonstrated the antioxidant activity of C. hystrix water extract and its effect on human keratinocyte and fibroblast migration. The extract showed a high amount of phenolic and flavonoid contents. The HPLC analysis indicated the presence of gallic acid, catechin, caffeic acid, rutin, and quercetin. We showed that C. hystrix water extract exhibited free radical scavenging capacity, determined by DPPH assay, with IC 50 of 14.91 mg/mL, and nitrite radical scavenging capacity, determined by NO assay, with IC 50 of 4.46 mg/mL. The C. hystrix water extract displayed unnoticeable toxicity at all tested doses. We showed that the treatment of water extracts as low as 50 μg/mL decreased the reactive oxygen species (ROS) from H 2 O 2-induced ROS formation in both cell lines. Besides, C. hystrix water extract promoted cell migration in a dose-dependent manner. Together, these results demonstrated the positive benefit of C. hystrix water extract as a wound-healing accelerator. Its health benefits may be due to the antioxidant capability of its phytochemical compounds contained in C. hystrix water extract that enhances the migration of two major cell types: fibroblast and keratinocytes, responsible for the proliferation and remodeling phase of wound healing.

Comparison of salivary cortisol, amylase, and chromogranin A diurnal profiles in healthy volunteers

Archives of Oral Biology

Oxidative Medicine and Cellular Longevity, 2021

The impairment in the regulation of the physiological process in the inflammatory phase of wound ... more The impairment in the regulation of the physiological process in the inflammatory phase of wound healing results in oxidative stress damage, which increases the severity and extends the healing time. In this study, we aimed to evaluate the radical scavenging properties of Coccinia leaf extract and its ability to ameliorate a migration process in vitro. Coccinia is a medicinal plant that was used in ancient times for relieving insect bite itching and swelling. However, the role of Coccinia leaf extract as an antioxidant related to the process of wound healing has never been studied. In this study, we demonstrated that the leaf extract possessed antioxidant properties that acted as a proton donor to neutralize reactive oxygen species with the IC50 value of 4.85 mg/mL of the extract. It could chelate iron with the IC50 value of 21.39 mg/mL of the extract. The leaf extract protected the human fibroblasts and keratinocytes from hydrogen peroxide-induced oxidative stress by increasing cel...

Thermal Steam Aerosolization Protects Against Lipopolysaccharide-Induced Sepsis in Rats

Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2016

Background Sepsis is a severe systemic inflammatory state in response to infection. The induction... more Background Sepsis is a severe systemic inflammatory state in response to infection. The induction of heat shock protein 70 (HSP70) by heat stress has been reported to protect against lethal effects of sepsis. In clinical situation, inhalation of thermal water has been used empirically in the treatment of chronic diseases of respiratory tract. Thus, thermal steam aerosolization may have beneficial effects on sepsis via HPS70 induction. Objective The present study tested the hypothesis that thermal steam aerosolization could protect against lipopolysaccharideinduced sepsis in rats. Material and Method Male Spraque-Dawley rats were subjected to steam aerosolization at 40°C for 1 hour before intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS). Blood pressure, heart rate and core temperature were recorded in two hours interval. At the end of the study period, vascular response to vasoconstrictor of isolated aortic rings ex vivo was assessed. Serum proinflammatory cytokines, IL...

Antioxidative Effects of Pueraria mirifica in Cholesterol-Fed Rabbits

White Kwao Krua (Pueraria mirifica) is a thai rejuvenating folk medicine which contains many phyt... more White Kwao Krua (Pueraria mirifica) is a thai rejuvenating folk medicine which contains many phytoestrogens including miroestrol, deoxymiroestrol and isoflavonoid group like kwakhurin, kwakhurin hydrate, daidzein, genistein which posses estrogenic-like activities. This study aimed to investigate the effect of White Kwao Krua on cholesterol level and the resistance of low density lipoprotein (LDL) to oxidation promoted by copper ion ex vivo in cholesterol-fed rabbits. Rabbits were fed diet containing no additive (control), 1% cholesterol (cholesterol group) or 1 % cholesterol with 10 mg/kg/day extracted-White Kwao Krua (White Kwao Krua group) for 12 weeks. Plasma total cholesterol level (T-chol), LDL-cholesterol (LDL-chol), high density lipoprotein-cholesterol (HDL-chol) and triglyceride were measured before treatment and every 4 weeks thereafter. White Kwao Krua reduced plasma T-chol (6% lower), LDL-chol (7% lower) and triglyceride (6% lower) significantly, but no altered HDL-chol l...

Pharmacological Characterization of the NMDA Receptor in the Human Platelet

N-methyl-D-aspartate (NMDA) roceptors have critical roles in excitatory synaptic transmission, pl... more N-methyl-D-aspartate (NMDA) roceptors have critical roles in excitatory synaptic transmission, plasticity and excitotoxicity in the CNS. These receptors also have been implicated in many physiological processes such as information processing; learning and memory, and in pathological processes such as hypoxia, degenerative diseases and drug-addicted brain damage. More recently, NMDA receptors were found in non-neuronal tissues such as bone, pancreas and skin. This study aimed to investigate NMDA receptor in human platelet. By using platelet aggregation study, the MK801 which is a non-competitive antagonist in the channel of NMDA receptor can inhibit platelet aggregation induced by ADP around 40-50%. By using radioligand binding study with centrifugation technique, [ 3 H] MK801 can bind to platelet with high affinity (Kd 27.99+/-6.12 nM, Bmax 888.76 +/-67.95 fmol/mg protein). The displacement of 0 . 5 nM [ 3 H] MK801 in platelet by channel blockers was monophasic (rank order: MK801&gt...

Study on organic acid and anti-oxidant properties of Elaeagnus latifolia L

Effect of Temperature and Feed Solution on Microencapsulation of Quercetin by Spray Drying Technique

World Academy of Science, Engineering and Technology, International Journal of Nutrition and Food Engineering, 2016

The Anti-apoptosis Effect of Curcumin I on bax/bcl-2 Ratio Against 6-OHDA Induced SH-SH5Y Toxicity

Curcumin is a naturally occurring polyphenolic compound. It has been reported that it exerts anti... more Curcumin is a naturally occurring polyphenolic compound. It has been reported that it exerts anti-oxidative and anti-apoptotic activities. 6-Hydroxydopamine (6-OHDA) is used as a neurotoxin to generate a model of Parkinson’s disease. In the present study, we investigate whether curcumin I (diferuloylmethane) could protect SH-SY5Y, dopaminergic cells from 6- OHDA-induced neurotoxicity. The results showed that pretreatment with curcumin I significantly prevented 6-OHDA induced cell viability reduction. Further experiments indicated that curcumin I could protect 6-OHDA-induced apoptosis signaling cascades activation. The results showed that pretreatment with curcumin I could prevent 6-OHDAinduced increasing of Bax/Bcl-2 ratio. This study suggested that curcumin I exerts its protective effects against 6-OHDA induced neurotoxicity. Therefore, curcumin I may be used as a potential compound for preventing oxidative stress-induced neurodegeneration.

Journal of Traditional and Complementary Medicine, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Neuroscience letters, Feb 5, 2018

γ-Mangostin is a xanthone with hydroxyl groups that confer the substance-free radical scavenging ... more γ-Mangostin is a xanthone with hydroxyl groups that confer the substance-free radical scavenging effects. As opposed to the other more extensively studied mangostins, scarce research has been conducted on neuroprotective effects of γ-mangostin on models of Parkinson's disease (PD). Therefore, this investigation aimed to elucidate its antioxidant and neuroprotective effects on 6-OHDA-induced toxicity in SH-SY5Y cells. 6-OHDA treatment, an inducer of PD pathology in vitro studies, decreased cell viability and increased the level of intracellular ROS production. Furthermore, the substance-induced the expression of phosphorylated p38 MAPK, negatively affected the Bax/Bcl-2 ratio and increased caspase-3 activity; all of which were factors that are associated with apoptosis. Pretreatment of cells with γ-mangostin at concentrations of 0.5, 1, and 2.5μM markedly increased cell survival and reduced the level of intracellular ROS formation as shown by DPPH radical scavenging activity of t...

Wnt signaling inhibitor FH535 selectively inhibits cell proliferation and potentiates imatinib-induced apoptosis in myeloid leukemia cell lines

International Journal of Hematology, 2016

Wnt signaling pathway plays a major role in leukemogenesis of myeloid leukemia. Aberrancy in its ... more Wnt signaling pathway plays a major role in leukemogenesis of myeloid leukemia. Aberrancy in its regulation results in hyperactivity of the pathway contributing to leukemia propagation and maintenance. To investigate effects of Wnt pathway inhibition in leukemia, we used human leukemia cell lines (i.e., K562, HL60, THP1, and Jurkat) and several Wnt inhibitors, including XAV939, IWP2 and FH535. Our results showed that leukemia cell lines (&amp;gt;95 % cells) had increased endogenous levels of β-catenin as compared to mononuclear cells from healthy donors (0 %). Among the tested inhibitors, FH535 demonstrated a markedly suppressive effect (IC50 = 358 nM) on mRNA levels of β-catenin target genes (LEF1, CCND1, and cMYC). In addition, FH535 significantly potentiated imatinib-induced apoptosis. Evaluation of erythrocyte and megakaryocyte lineage using flow cytometry demonstrated that the potentiation mechanism is independent of the developmental stage, and is more likely due to crosstalk between other pathways and β-catenin. FH535 also displayed antiproliferative properties in other cell lines used in this study. In summary, FH535 showed significantly high antiproliferative effects at submicromolar dosages, and additionally enhanced imatinib-induced apoptosis in human leukemia cell lines. Our results highlight its potential antileukemic promise when used in conjunction with other conventional therapeutic regimens.

Study on Physical Properties, Hydrolyzable Tannin Contents and Free Radical Scavenging Activities of Phyllanthus emblica Linn. from Kanchanaburi Province

วารสารเภสัชวิทยา, 2013

Phyllanthus emblica Linn. (Indian gooseberry) is an indigenous fruit crop growing in all region o... more Phyllanthus emblica Linn. (Indian gooseberry) is an indigenous fruit crop growing in all region of Thailand. The fruit has antioxidant properties and have been used as both food and herbal medicine. The objectives of this work were to study the physical properties, hydrolysable tannin contents and antioxidant activity of 12 selected clones of Indian gooseberry from Kanchanaburi province. The results of the study showed significant differences ( p <0.05) in all physical properties including fruit diameter (2.46±0.2-3.38±0.1 cm), fruit height (2.43±0.1-3.03±0.1 cm), fresh weight (8.47±1.1-20.67±1.8 g), percentage of flesh weight (85.48±1.8-92.38±1.0%) and percentage of flesh dry matter (13.21±2.6-17.14±1.2%). Among all clones of Indian gooseberry, PK1, PK8 and PK16 had the highest fruit fresh weights. The results also demonstrated significant differences ( p <0.05) in the contents of hydrolyzable tannin and antioxidant activity among 12 selected clones of Indian gooseberry. The contents of hydrolyzable tannin and DPPH radical scavenging activity in all Indian gooseberry varied from 192.8±8.2-445.9±8.4 mg tannic acid equivalents/g of dry weight and antioxidant activity (IC 50 ) varied from 0.194-0.807. PK5 have highest hydrolyzable tannin contents and antioxidant properties. It was concluded that antioxidant properties of Indian gooseberry varied in accordance with tannin contents of the fruits.

Curcumin I protects the dopaminergic cell line SH-SY5Y from 6-hydroxydopamine-induced neurotoxicity through attenuation of p53-mediated apoptosis

Neuroscience Letters, 2011

Oxidative stress (OS) plays a pivotal role in the pathogenesis of Parkinson&amp;amp;amp;a... more Oxidative stress (OS) plays a pivotal role in the pathogenesis of Parkinson&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s disease (PD). 6-Hydroxydopamine (6-OHDA) is a neurotoxin used to induce oxidative cell death of dopaminergic neurons in experimental models of PD. Curcumin I, or diferuloylmethane is a pure compound isolated from Curcuma longa Linn. that has been reported to have neuroprotective properties. The precise mechanism, however, remains unclear. This study aims to elucidate the mechanisms by which curcumin I exerts its effects, using 6-OHDA-induced neurotoxicity in the human dopaminergic cell line SH-SY5Y. In our experiments, pretreatment with curcumin I improved cell viability, and significantly reduced reactive oxygen species (ROS). Further investigations revealed a reduction of p53 phosphorylation and decrease of the Bax/Bcl-2 ratio, as measured by mRNA expression and protein level. Taken together, these findings indicate that curcumin I protects dopaminergic neurons from 6-OHDA-induced toxicity via the reduction of ROS production, and subsequent attenuation of p53 phosphorylation and reduction of the Bax/Bcl-2 ratio.

Neuroscience Letters, 2011

Ya-Ba, a combination of the two potent psychostimulants methamphetamine (METH) and caffeine (CAF)... more Ya-Ba, a combination of the two potent psychostimulants methamphetamine (METH) and caffeine (CAF), is commonly used by drug abusers in Thailand and neighboring countries. While the neurotoxic effects of METH are well documented, the toxicity of this combination is mostly unknown. This study aimed to elucidate the effects of this particular drug combination using both in vitro and in vivo models. We found that combined treatment of METH and CAF at individually non-toxic concentrations significantly decreased viability of human neuroblastoma SK-N-SH cells. The reduction in cell survival was accompanied by an increase in reactive oxygen species (ROS) formation and the Bax/Bcl-2 ratio. In vivo data showed that combined administration of METH and CAF increased the mortality rate of rats, with an increase in the level of thiobarbituric acid reactive substances (TBARS), the indicator of oxidative stress, in striatal tissues. The results indicate that caffeine potentiates the toxic effects of methamphetamine, possibly via a mechanism involving an increase in dopamine release and excess ROS generation.

Journal of Ethnopharmacology, 2008

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activitie... more The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

ScienceAsia, 2016

Oxidative stress plays an important role in the pathogenesis of Parkinson's disease. The neurotox... more Oxidative stress plays an important role in the pathogenesis of Parkinson's disease. The neurotoxin 6-hydroxydopamine initiates cellular oxidative stress which has been implicated in neurodegenerative conditions related to Parkinson's disease. Eclipta prostrata, also known as false daisy, is a plant belonging to the sunflower family. It is a rich source of phenolic compounds that confer the plant an antioxidant activity. In this study, we investigated the protective effects of ethyl acetate extract of E. prostrata against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. Pretreatment with the ethyl acetate extract of E. prostrata before exposing the cells to 6-hydroxydopamine significantly increased cell survival and reduced intracellular reactive oxygen species formation in a concentrationdependent manner. In addition, the ethyl acetate extract of E. prostrata suppressed the Bax/Bcl-2 ratio and reduced caspase-3 activity, a key modulator of cell apoptosis. Our finding clearly shows that the ethyl acetate extract of E. prostrata possesses a potential antioxidant capacity and attenuates SH-SY5Y cell death by blocking apoptotic signalling caused by 6-hydroxydopamine. E. prostrata could therefore be used as a potential herb to prevent oxidative stress-induced neurodegeneration in Parkinson's disease.