Raunel Tinoco - Academia.edu (original) (raw)

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Papers by Raunel Tinoco

Biotechnology Letters, 2014

Fuel Processing Technology, 1998

Aquaculture Nutrition, 2008

Applied and environmental microbiology, 1999

We studied the metabolism of polycyclic aromatic hydrocarbons (PAHs) by using white rot fungi pre... more We studied the metabolism of polycyclic aromatic hydrocarbons (PAHs) by using white rot fungi previously identified as organisms that metabolize polychlorinated biphenyls. Bran flakes medium, which has been shown to support production of high levels of laccase and manganese peroxidase, was used as the growth medium. Ten fungi grown for 5 days in this medium in the presence of anthracene, pyrene, or phenanthrene, each at a concentration of 5 microg/ml could metabolize these PAHs. We studied the oxidation of 10 PAHs by using laccase purified from Coriolopsis gallica. The reaction mixtures contained 20 microM PAH, 15% acetonitrile in 60 mM phosphate buffer (pH 6), 1 mM 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), and 5 U of laccase. Laccase exhibited 91% of its maximum activity in the absence of acetonitrile. The following seven PAHs were oxidized by laccase: benzo[a]pyrene, 9-methylanthracene, 2-methylanthracene, anthracene, biphenylene, acenaphthene, and phenanthrene...

Lignin peroxidase, cytochrome c and haemoglobin were tested for oxidation of polycyclic aromatic ... more Lignin peroxidase, cytochrome c and haemoglobin were tested for oxidation of polycyclic aromatic hydrocarbon (PAH) in the presence of hydrogen peroxide. The reaction mixture contained water-miscible organic solvents in order to reduce the mass transfer limitation of ...