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Research paper thumbnail of Cloning and Characterization of Cucumber Mosaic Virus Coat Protein Gene from Infected Banana Plants in Egypt

Egyptian Journal of Genetics and Cytology, 2013

ucumber mosaic virus (CMV) is an important plant virus, affecting hundreds of plant species and c... more ucumber mosaic virus (CMV) is an important plant virus, affecting hundreds of plant species and causing numerous diseases (Palukaitis et al., 1992). CMV is the type member of the genus Cucumovirus, family Bromoviridae. It is one of the most economically important plant viruses in the world because it causes great losses in vegetables, ornamentals and fruits. The destructive effect is due to its rapid spread by more than 60 aphid species vectors in the field (Edwardson and Christie, 1991). The CMV genome is organized into three single-stranded messenger-sense genomic RNAs (RNAs 1, 2 and 3) and two major subgenomic RNAs (RNAs 4 and 4A, which serve for the expression of the 3′proximal gene of RNAs 3 and 2, respectively). RNAs 1 and 2 codes for components of the replicase complex. RNA 2 codes for the 2b protein, which is expressed from subgenomic RNA 4A and is involved in the suppression of gene silencing. RNA 3 encodes the 3a protein, which is essential for virus movement (Palukaitis and Garcia-Arenal, 2003). The coat protein (CP) is expressed from RNA4 (Habili and Francki, 1974; Palukaitis and Garcia-Arenal, 2003). The CP is required for host

Research paper thumbnail of Cloning and Characterization of Cucumber Mosaic Virus Coat Protein Gene from Infected Banana Plants in Egypt

Egyptian Journal of Genetics and Cytology, 2013

ucumber mosaic virus (CMV) is an important plant virus, affecting hundreds of plant species and c... more ucumber mosaic virus (CMV) is an important plant virus, affecting hundreds of plant species and causing numerous diseases (Palukaitis et al., 1992). CMV is the type member of the genus Cucumovirus, family Bromoviridae. It is one of the most economically important plant viruses in the world because it causes great losses in vegetables, ornamentals and fruits. The destructive effect is due to its rapid spread by more than 60 aphid species vectors in the field (Edwardson and Christie, 1991). The CMV genome is organized into three single-stranded messenger-sense genomic RNAs (RNAs 1, 2 and 3) and two major subgenomic RNAs (RNAs 4 and 4A, which serve for the expression of the 3′proximal gene of RNAs 3 and 2, respectively). RNAs 1 and 2 codes for components of the replicase complex. RNA 2 codes for the 2b protein, which is expressed from subgenomic RNA 4A and is involved in the suppression of gene silencing. RNA 3 encodes the 3a protein, which is essential for virus movement (Palukaitis and Garcia-Arenal, 2003). The coat protein (CP) is expressed from RNA4 (Habili and Francki, 1974; Palukaitis and Garcia-Arenal, 2003). The CP is required for host

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