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Papers by Reetesh Pratap Singh

Journal of experimental agriculture international, Apr 4, 2024

Green lacewing, Chrysoperla zastrowi (Esben-Petersons) (Neuroptera: Chrysopidae) is the most effe... more Green lacewing, Chrysoperla zastrowi (Esben-Petersons) (Neuroptera: Chrysopidae) is the most effective polyphagous predator of different species of aphids and is commonly known as "aphid lion." During the study, the green lacewing adults were collected from the flowers of different crop plants in Meerut region. The experiment on feeding potential of green lacewing was studied in the

International Journal of Environment and Climate Change

The chickpea pod borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a destructive pe... more The chickpea pod borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a destructive pest of chickpeas that is difficult to control using conventional methods. We isolated and evaluated the virulence of five isolates of Metarhizium anisopliae against larvae of H. armigera. All isolates of M. anisopliae, SVPUAT 1, SVPUAT 2, SVPUAT 3, SVPUAT 4, and SVPUAT 5, were most effective against the second instar H. armigera at 2 ×108 conidia/ml. Among all isolates, SVPUAT 1 Accession no. ON183248 had the highest virulence 100% mortality, whereas LT50 and LT90 were 3.16 and 5.16 days.

International Journal of Environment and Climate Change

The experiment on biology of fruit borer Helicoverpa armigera (Hubner) was carried out in the Bio... more The experiment on biology of fruit borer Helicoverpa armigera (Hubner) was carried out in the Bio-control Laboratory, Department of Entomology, S.V.P.U.A.&T., Meerut. Results showed that the mean pre-oviposition, oviposition and post-oviposition period of fruit borer on tomato lasted for 2.46 ± 0.29, 6.33 ± 0.14 and 1.67 ± 0.14 days, respectively. A female laid on an average 329.40 ± 68.17 eggs. The incubation period was observed to be 3.08 ± 0.32 days on tomato. The first instar larva was very active with body length of 1.54 ± 0.81 mm and body breadth of 0.46 ± 0.05 mm. The larval development completed within 22.45 ± 0.44 days. The duration of each instar on tomato was found to be 2.58 ± 0.26, 3.50 ± 0.14, 3.42 ± 0.26, 3.71 ± 0.28, 4.38 ± 0.16 and 4.83 ± 0.14 days, respectively. The mean pre-pupal period lasted for 2.00 ± 0.24 days on tomato. The mean pupal period lasted for 12.33 ± 0.62 days. Longevity of adult ranged from 8 to 11 days with an average of 9.30 ± 1.25 days in males,...

Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a signif... more Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for acquiring complete transcriptome information of insect species when there is no reference genome available. In case of Hellula undalis, only 17 nucleotide records are currently available throughout NCBI nucleotide database. Genes associated with metabolic processes, general development, reproduction, defense and functional genomics were not previously predicted in the Hellula undalis at the genomic level. Methods & Results To address this issue, we constructed Hellula undalis transcriptome using Illumina NovaSeq6000 technology. Approximately 48 million 125 bp paired-end reads were obtained from sequencing. A total of 30,451 contigs were ge...

Egyptian Journal of Biological Pest Control, 2020

The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative optio... more The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative option for high-risk insecticides. Beauveria bassiana is one of the most potential EPF of pest control all over the world. The EPF isolate Y-F_ITS1 was isolated from Egyptian fauna on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolate, using PCR amplification with B. bassiana-specific primers and nucleotide sequence analysis of the internal transcribed spacer (ITS) region, confirmed the isolate as B. bassiana (accession no. [MK773644.1]). Efficacy of the isolate Y-F_ITS1 was examined against fourth instar larvae of Galleria mellonella L. as a model insect using concentrations from 104 to 107 spores ml−1 in a contact toxicity assay under laboratory conditions. The pathogenicity experiment showed that all the tested concentrations caused mortalities ranged from 75 to 98.33%, within 5 days posttreatment with cumulative mortalities, reached 100%, befor...

Journal of experimental agriculture international, Apr 4, 2024

Green lacewing, Chrysoperla zastrowi (Esben-Petersons) (Neuroptera: Chrysopidae) is the most effe... more Green lacewing, Chrysoperla zastrowi (Esben-Petersons) (Neuroptera: Chrysopidae) is the most effective polyphagous predator of different species of aphids and is commonly known as "aphid lion." During the study, the green lacewing adults were collected from the flowers of different crop plants in Meerut region. The experiment on feeding potential of green lacewing was studied in the

International Journal of Environment and Climate Change

The chickpea pod borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a destructive pe... more The chickpea pod borer Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a destructive pest of chickpeas that is difficult to control using conventional methods. We isolated and evaluated the virulence of five isolates of Metarhizium anisopliae against larvae of H. armigera. All isolates of M. anisopliae, SVPUAT 1, SVPUAT 2, SVPUAT 3, SVPUAT 4, and SVPUAT 5, were most effective against the second instar H. armigera at 2 ×108 conidia/ml. Among all isolates, SVPUAT 1 Accession no. ON183248 had the highest virulence 100% mortality, whereas LT50 and LT90 were 3.16 and 5.16 days.

International Journal of Environment and Climate Change

The experiment on biology of fruit borer Helicoverpa armigera (Hubner) was carried out in the Bio... more The experiment on biology of fruit borer Helicoverpa armigera (Hubner) was carried out in the Bio-control Laboratory, Department of Entomology, S.V.P.U.A.&T., Meerut. Results showed that the mean pre-oviposition, oviposition and post-oviposition period of fruit borer on tomato lasted for 2.46 ± 0.29, 6.33 ± 0.14 and 1.67 ± 0.14 days, respectively. A female laid on an average 329.40 ± 68.17 eggs. The incubation period was observed to be 3.08 ± 0.32 days on tomato. The first instar larva was very active with body length of 1.54 ± 0.81 mm and body breadth of 0.46 ± 0.05 mm. The larval development completed within 22.45 ± 0.44 days. The duration of each instar on tomato was found to be 2.58 ± 0.26, 3.50 ± 0.14, 3.42 ± 0.26, 3.71 ± 0.28, 4.38 ± 0.16 and 4.83 ± 0.14 days, respectively. The mean pre-pupal period lasted for 2.00 ± 0.24 days on tomato. The mean pupal period lasted for 12.33 ± 0.62 days. Longevity of adult ranged from 8 to 11 days with an average of 9.30 ± 1.25 days in males,...

Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a signif... more Background The cabbage webworm, Hellula undalis (Fabricius) (Lepidoptera: Pyralidae), is a significant pest of brassicas and other cruciferous plants in warm regions worldwide. Transcriptome analysis is valuable for investigation of molecular mechanisms underlying the insect development and reproduction. De novo assembly is particularly useful for acquiring complete transcriptome information of insect species when there is no reference genome available. In case of Hellula undalis, only 17 nucleotide records are currently available throughout NCBI nucleotide database. Genes associated with metabolic processes, general development, reproduction, defense and functional genomics were not previously predicted in the Hellula undalis at the genomic level. Methods & Results To address this issue, we constructed Hellula undalis transcriptome using Illumina NovaSeq6000 technology. Approximately 48 million 125 bp paired-end reads were obtained from sequencing. A total of 30,451 contigs were ge...

Egyptian Journal of Biological Pest Control, 2020

The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative optio... more The entomopathogenic fungi (EPF), as a biological control agent, can provide an alternative option for high-risk insecticides. Beauveria bassiana is one of the most potential EPF of pest control all over the world. The EPF isolate Y-F_ITS1 was isolated from Egyptian fauna on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolate, using PCR amplification with B. bassiana-specific primers and nucleotide sequence analysis of the internal transcribed spacer (ITS) region, confirmed the isolate as B. bassiana (accession no. [MK773644.1]). Efficacy of the isolate Y-F_ITS1 was examined against fourth instar larvae of Galleria mellonella L. as a model insect using concentrations from 104 to 107 spores ml−1 in a contact toxicity assay under laboratory conditions. The pathogenicity experiment showed that all the tested concentrations caused mortalities ranged from 75 to 98.33%, within 5 days posttreatment with cumulative mortalities, reached 100%, befor...