Regina G Palma-dibb - Academia.edu (original) (raw)

Papers by Regina G Palma-dibb

Research paper thumbnail of Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface

Brazilian Dental Journal

Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen ... more Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months stor...

Research paper thumbnail of Avaliação clínica de diferentes métodos de diagnóstico de lesões de cárie de superfícies proximal e oclusal

Research paper thumbnail of Assessing the surface roughness of a posterior resin composite: effect of surface sealing

Operative dentistry

This study assessed the in vitro influence of surface sealing on the surface roughness of a poste... more This study assessed the in vitro influence of surface sealing on the surface roughness of a posterior resin composite before and after toothbrushing. Thirty specimens (13 mm diameter x 1 mm high) were fabricated from Filtek-P60 resin composite and randomly assigned to three groups (n = 10): a non-sealed control and two groups sealed with one of the tested materials-a surface-penetrating sealant (Protect-it!-PI) and a one bottle adhesive system (Single Bond-SB). The samples were subjected to a surface roughness reading to determine the initial roughness, then submitted to simulated toothbrushing with 35,600 cycles for 100 minutes. Specimens were then cleaned and a post-abrasion surface roughness reading accomplished. Means (microm), recorded before (B) and after (A) toothbrushing, and standard deviations were: Control--(B): 0.032 (+/- 0.005), (A): 0.054 (+/- 0.005); PI-(B): 0.034 (+/- 0.005), (A): 0.060 (+/- 0.034); SB (B): 0.031 (+/- 0.004), (A): 0.047 (+/- 0.007). Data were tabulat...

Research paper thumbnail of I nfluence of salivary contamination on marginal microleakage of pit and fissure sealants

Research paper thumbnail of Efficiency and effectiveness of Er: YAG laser on carious tissue removal

Research paper thumbnail of Assessing microleakage in resin composite restorations rebonded with a surface sealant and three low-viscosity resin systems

Quintessence international (Berlin, Germany : 1985), 2002

The aim of this study was to assess the ability of three low-viscosity resin systems (Unibond, Si... more The aim of this study was to assess the ability of three low-viscosity resin systems (Unibond, Single Bond, and Fluorseal), used as rebonding agents, and a specific surface-penetrating sealant (Fortify) to prevent microleakage in Class V resin composite restorations. Fifty Class V cavities with the occlusal margin in enamel and the cervical margin in dentin and cementum were prepared on both the buccal and lingual surfaces of sound extracted premolars and restored with a hybrid light-cured resin composite. After finishing and polishing, restorations were randomly assigned to one of five equal groups (n = 10): a control, without surface sealing, and four experimental groups in which margins were etched and rebonded. Specimens were thermocycled, immersed in a 50% silver nitrate solution, sectioned longitudinally, and analyzed for leakage at the occlusal and cervical interfaces. Statistical analysis showed significantly less leakage at the enamel margins for all groups. Fortify and Uni...

Research paper thumbnail of Mechanical properties and bond strength of glass-ionomer cements

The journal of adhesive dentistry, 2002

The objective of this study was to evaluate the mechanical properties and bond strength of glass-... more The objective of this study was to evaluate the mechanical properties and bond strength of glass-ionomer cements (GICs) and resin-modified GICs (RM-GICs) that are indicated as restorative materials for the Atraumatic Restorative Treatment (ART) technique. Fifteen disk specimens for the diametral tensile strength (DTS) test and fifteen cylindrical specimens for the compressive strength (CS) test were made of each GIC: Ketac-Fil, Ketac-Molar (ESPE), Fuji IX and Fuji PLUS (GC). Forty human molars were sectioned and embedded in resin with either buccal or lingual surfaces exposed for the tensile bond strength (TBS) test. The surface was ground until a flattened area of enamel or dentin was obtained. After conditioning, inverted truncated cones of GICs were prepared on the flat tooth surfaces. The powder:liquid ratio of Fuji PLUS was adjusted for restorative purposes. Prior to testing, specimens were stored for 24 h (TBS test) and for 1 h, 24 h, and 7 days (CS and DTS tests) in deionized...

Research paper thumbnail of Avaliação quantitativain vitro'da microinfiltração marginal em restaurações Classe V em resina composta, utilizando-se sistemas adesivos total e self-etching; …

Rev. paul. odontol, 2004

Resumo: O objetivo do presente estudo foi avaliar quantitativamente in vitro a microinfiltração m... more Resumo: O objetivo do presente estudo foi avaliar quantitativamente in vitro a microinfiltração marginal em restaurações classe V em resina composta, utilizando-se diferentes sistemas adesivos: Clearfill SE Bond (Kuraray), One-up bond F (Toruyama) e ...

Research paper thumbnail of Bonding performance of different adhesive systems to deproteinized dentin: Microtensile bond strength and scanning electron microscopy

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005

Deproteinization has been shown to optimize dentin bonding, but differences in adhesive compositi... more Deproteinization has been shown to optimize dentin bonding, but differences in adhesive composition should be considered. The objective of this study was to evaluate the effect of dentin deproteinization on microtensile bond strength (microTBS) of four total-etch adhesive systems (Single Bond/SB, Prime & Bond NT/PB, One Coat Bond/OC, and PQ1/PQ). The ultrastructure of the resin-dentin interfaces was also examined using scanning electron microscopy. Tukey's multiple-comparison tests indicated that PB and PQ produced significantly higher microTBS (p<0.05) after dentin deproteinization (PB=61.53 MPa, PQ=58.18 MPa). This treatment provided statistically lower results for SB (39.08 MPa), but the microTBS of OC to dentin was unaffected by dentin deproteinization. The bonding performance on deproteinized dentin surfaces depended on the characteristics of each adhesive system, as well as the adhesive dentin specificity to the oxidant effect of sodium hypochlorite. Incorporation of fillers in the adhesive, a possible self-etching action, and the presence of a volatile solvent (acetone) were the main factors for a better union between the adhesive system and deproteinized substrate.

Research paper thumbnail of Clareamento caseiro de dentes vitais; Home vital bleaching

Research paper thumbnail of Avaliação da eficiência de desgaste de pontas diamantadas

O objetivo do presente estudo foi avaliar a eficiencia de desgaste sobre dentes bovinos de pontas... more O objetivo do presente estudo foi avaliar a eficiencia de desgaste sobre dentes bovinos de pontas diamantada de diferentes marcas comerciais (KG Sorensen, Microdont, Fava e SSWhite). Para tal finalidade, foram testadas 10 pontas de cada marca comercial, cujo substrato para o desgaste constituia-se de incisivos bovinos, seccionados em 4 partes iguais (2 cervicais e 2 incisais) e incluidos em resina de poliester, totalizando 4 especimes para cada instrumento rotatorio. Para padronizacao do desgaste, foi utilizado um dispositivo, no qual acoplou-se a caneta de alta-rotacao. Todos os testes foram executados sob refrigeracao constante e as pontas foram divididas aleatoriamente e sem identificacao. Os dados foram analisados atraves da analise de variância a dois criterios (p>0,01) e teste de Tukey (p>0,05). Pode-se observar que as pontas diamantadas KG Sorensen demonstraram estatisticamente maior eficiencia no desgaste do que as demais pontas testadas, sendo mais evidenciado apos o 2° seccao de dente. Atraves dos resultados obtidos, pode-se concluir que as pontas diamantadas KG apresentaram melhor desempenho tanto na porcao incisal quanto na cervical. Termos de Indexacao: pontas diamantadas; eficiencia de desgaste; estrutura denta.

Research paper thumbnail of Análise quantitativa in vitro da microinfiltração marginal em restaurações classe V com materiais restauradores adesivos. Influência do uso do laser de Er: YAG no preparo cavitário

Research paper thumbnail of Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals

Research paper thumbnail of Changes in enamel after bleaching pre-treatment with non-thermal atmospheric plasma

Clinical Plasma Medicine

Abstract The aim of this study was to evaluate color change, mineral composition and topography o... more Abstract The aim of this study was to evaluate color change, mineral composition and topography of the enamel after the pre-treatment with non-thermal atmospheric plasma (NTAP) followed by 38% hydrogen peroxide (HP) bleaching. Buccal enamel of bovine incisors teeth was stained with black tea solution and then divided into five groups (n = 12), according to the plasma application time: PL1 (one minute) or PL2 (2 min) and number of HP applications: HP1 (one) and HP3 (three). The following groups were investigated: HP3, PL1+HP1, PL1+HP3, PL2+HP1 and PL2+HP3. Four color measurements: initial (untreated), after staining and after bleaching (first and second sessions), were performed using an intraoral spectrophotometer (Vita Easyshade). Energy Dispersive X-ray Spectroscopy (EDX) was used to analyze the mineral composition of the enamel before and after two bleaching sessions. Laser confocal microscopy was used to analyze the enamel topography. The pre-treatment with NTAP did not improve the whitening results. The use of NTAP preserves the concentration of calcium and phosphorus, only HP3 group experimented a significant decrease in the mineral concentration. All the bleached groups, with or without NTAP treatment increased their enamel roughness. The application of NTAP did not influence the color change, but it was important to maintain the mineral composition of bleached teeth. All the groups increased their roughness level.

Research paper thumbnail of Laser managed in the treatment of hypomineralized occlusal defects in teeth enamel affected by molar-incisor hypomineralization: A randomized controlled clinical study / Uso do laser no tratamento dos defeitos oclusais hipomineralizados em esmalte de dentes afetados pela hipomineralização molar-i...

Brazilian Journal of Health Review

Background: Nowadays, observed a growing rate of teeth affected by molar incisor hypomineralizati... more Background: Nowadays, observed a growing rate of teeth affected by molar incisor hypomineralization (MIH). These teeth can present persistent inflammation of the pulp, increasing the dentin hypersensitivity and consequently, directly affecting the cleaning of the affected tooth and contributing to the progression of caries lesions. Aims: This study aims to compare the use of diode laser (DL) irradiation (970nm) with the application of a glass ionomer-based sealant (GIC) for the most clinically and cost-effective strategy for the management of first permanent molars affected with MIH. Methods: This is a two-arm, parallel-group, patient randomized superiority-controlled trial, children present MIH molars will be selected and treated either with the application of DL or with the application of GIC-based sealants. Baseline and follow-up evaluations will be assessed through clinical examination and analysis of dental impressions that will be carried out of the affected teeth. The outcome...

Research paper thumbnail of Effect of manual and electrical brushing on the enamel of sound primary teeth and teeth with induced white spot lesions

American journal of dentistry, 2020

PURPOSE To evaluate the effect of different electrical brushing systems on the surface roughness ... more PURPOSE To evaluate the effect of different electrical brushing systems on the surface roughness and wear profile of the enamel of sound primary teeth and teeth with induced white spot lesions. METHODS 45 specimens were obtained from sound primary incisors, and the buccal surface was divided into four parts: sound enamel; enamel with white spot lesions; sound enamel with brushing; and enamel with white spot lesions and brushing. Specimens were randomly divided into three groups (n =15), according to the different brushing systems: Group 1 - Electric rotating toothbrush (Kid's Power Toothbrush - Oral B); Group 2 - Sonic electric toothbrush (Baby Sonic Toothbrush); and Group 3 - Manual toothbrush (Curaprox infantil) (control). The specimens were analyzed for surface roughness and wear profile. Data were analyzed by appropriate statistical tests, with a significance level of 5%. RESULTS Regarding the surface roughness, no significant difference was observed between the groups. Howe...

Research paper thumbnail of Radiotherapy Activates Matrix Metalloproteinases in the Dentinoenamel Junction of Primary Teeth

Journal of dentistry for children, 2020

Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel ... more Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel junction (DEJ). We investigated the activity and expression of the matrix metalloproteinases (MMPs) -2 and -9 in order to understand disease pathogenesis in teeth submitted or not to radiotherapy (RT). Methods: In situ zymography and immunofluorescence assays were performed to evaluate the activity and expression of MMPs -2 and -9, respectively. Twelve primary second molars were randomly assigned into two experimental subgroups: irradiated and nonirradiated. Dental fragments were exposed to radiation at a dose fraction of two Gy for five consecutive days until reaching the total dose of 60 Gy. The percentage of fluorescence in the DEJ was evaluated in three distinct regions of the tooth (cervical, cusp, and pit). The regions were photographed under fluorescence microscopy at 1.25× and 5× magnification. Results: The intensity of fluorescence per mm 2 in the DEJ was higher in the cervical ...

Research paper thumbnail of Influence of Er,Cr:YSGG laser on dentin acid resistance after erosive challenge

American journal of dentistry, 2019

PURPOSE To evaluate the influence of the Er,Cr:YSGG laser with or without the 5% fluoride varnish... more PURPOSE To evaluate the influence of the Er,Cr:YSGG laser with or without the 5% fluoride varnish on the acid resistance of dentin after erosive challenge. METHODS 36 incisors were selected and sectioned, obtaining 72 specimens of 4 mm × 4 mm and randomly divided into eight groups (n = 9). In G1: application of Er,Cr:YSGG (0.1W; 5Hz, air 55%); G2: laser (0.25W; 5Hz, air 55%); G3: fluoride varnish + laser (0.1W; 5Hz, air 55%); G4: fluoride varnish + laser (0.25W, 5Hz, air 55%); G5: fluoride varnish + laser (0.1W; 5Hz, without air); G6: fluoride varnish + laser (0.25W, 5Hz, without air); G7: fluoride varnish and G8: no treatment. When used, the laser was irradiated without water cooling, scanning mode during 10 seconds. The surface roughness data were subjected to ANOVA. For wear profile, we used Kruskal-Wallis test and Dunn post-hoc, all with α= 0.05. RESULTS The results showed no statistically significant difference when comparing the groups as regards to the surface roughness (P&gt...

Research paper thumbnail of Effects of prolonged use of over‐the‐counter bleaching agents on enamel: An in vitro study

Microscopy Research and Technique, 2021

This study evaluated the effects of four over-the-counter (OTC) bleaching products on the propert... more This study evaluated the effects of four over-the-counter (OTC) bleaching products on the properties of enamel. Extracted human molars were randomly assigned into four groups (n = 5): PD: Poladay (SDI), WG: White Teeth Global (White Teeth Global), CW: Crest3DWhite (Procter & Gamble), and HS: HiSmile (HiSmile). The hydrogen peroxide (H2 O2 ) content in each product was analyzed via titration. Twenty teeth were sectioned into quarters, embedded in epoxy resin, and polished. Each quarter-tooth surface was treated with one of the four beaching times: T0: control/no-bleaching, T14: 14 days, T28: 28 days, and T56: 56 days. Materials were applied to enamel surfaces as recommended. Enamel surfaces were examined for ultramicrohardness (UMH), elastic modulus (EM), superficial roughness (Sa), and scanning electron microscopy (SEM). Ten additional teeth were used to evaluate color and degree of demineralization (DD) (n = 5). Data were statistically tested by two-way ANOVA and Tukey's tests (α = 5%). Enamel surfaces treated with PD and WG presented UMH values significantly lower than the controls (p < .05). Elastic modulus (E) was significantly reduced at T14 and T28 for PD, and at T14 for HS (p < .05). A significant increase in Sa was observed for CW at T14 (p < .05). Color changes were observed in the PD and WG groups. Additionally, DD analysis showed significant demineralization at T56 for CW. Overall, more evident morphological alterations were observed for bleaching products with higher concentrations of H2 O2 (p < .05), PD, and WG. Over-the-counter bleaching products containing H2 O2 can significantly alter enamel properties, especially when application time is extended.

Research paper thumbnail of Effects of aging on shear bond strength to dentin and mechanical properties of restorative glass ionomer cements

International Journal of Adhesion and Adhesives, 2020

Abstract Objectives To evaluate the shear bond strength to dentin (SBS), flexural strength (FS), ... more Abstract Objectives To evaluate the shear bond strength to dentin (SBS), flexural strength (FS), and diametral tensile strength (DTS) of four restorative glass ionomer cements after aging in artificial saliva. Materials and methods For SBS testing, sound molars were ground to flat occlusal dentin and fixed with acrylic resin. Teeth were randomly divided into four groups along with the following materials: Ketac Nano, Ketac Molar, Fuji II LC and Equia. Glass ionomer cylinders were bonded to each dentin surface. For testing the mechanical properties, FS bars (25 mm × 2 mm x 2 mm) and DTS discs (4 mm × 2 mm) were fabricated. All specimens were stored in artificial saliva (37 °C) and tested after 24 h and 6 months. Data was analyzed with two-way ANOVA and post-hoc Tukey's tests (α = 5%). Results The SBS results were statistically similar among the four glass ionomer cements (p > 0.05). No significant differences in SBS after 24-h and 6-month storage were found, except for in the Ketac Nano group, where a significant decrease in SBS was found after aging (p Conclusions Aging for 6 months did not affect the SBS of restorative glass ionomer cements, except in the case of Ketac Nano. All glass ionomer cements showed increased FS over time. The DTS remained unchanged – except for the Fuji II LC, which experienced increased DTS.

Research paper thumbnail of Phosphoric acid containing proanthocyanidin enhances bond stability of resin/dentin interface

Brazilian Dental Journal

Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen ... more Proanthocyanidin (PA) is a promising dentin biomodifier due to its ability to stabilize collagen fibrils against degradation by matrix metalloproteinases (MMPs); however, the most effective protocol to incorporate PA into bonding procedures is still unclear. This study evaluated the effect of dentin biomodification with a PA acid etchant on MMP activity, adhesive interface morphology and resin-dentin microtensile bond strength. Sound extracted human molars were flattened to expose dentin and acid-etched for 15 s according to the groups: EXP - experimental phosphoric acid; EXP+PA - experimental phosphoric acid 10% PA; TE - total-etching system; SE - self-etching system. Samples were restored with composite resin and stored in distilled water (37ºC). MMP activity and interface morphology were analyzed after 24 h by in situ zymography (n=6) and scanning electron microscopy (n=3), respectively. The resin-dentin microtensile bond strength (μTBS) was evaluated after 24 h and 6 months stor...

Research paper thumbnail of Avaliação clínica de diferentes métodos de diagnóstico de lesões de cárie de superfícies proximal e oclusal

Research paper thumbnail of Assessing the surface roughness of a posterior resin composite: effect of surface sealing

Operative dentistry

This study assessed the in vitro influence of surface sealing on the surface roughness of a poste... more This study assessed the in vitro influence of surface sealing on the surface roughness of a posterior resin composite before and after toothbrushing. Thirty specimens (13 mm diameter x 1 mm high) were fabricated from Filtek-P60 resin composite and randomly assigned to three groups (n = 10): a non-sealed control and two groups sealed with one of the tested materials-a surface-penetrating sealant (Protect-it!-PI) and a one bottle adhesive system (Single Bond-SB). The samples were subjected to a surface roughness reading to determine the initial roughness, then submitted to simulated toothbrushing with 35,600 cycles for 100 minutes. Specimens were then cleaned and a post-abrasion surface roughness reading accomplished. Means (microm), recorded before (B) and after (A) toothbrushing, and standard deviations were: Control--(B): 0.032 (+/- 0.005), (A): 0.054 (+/- 0.005); PI-(B): 0.034 (+/- 0.005), (A): 0.060 (+/- 0.034); SB (B): 0.031 (+/- 0.004), (A): 0.047 (+/- 0.007). Data were tabulat...

Research paper thumbnail of I nfluence of salivary contamination on marginal microleakage of pit and fissure sealants

Research paper thumbnail of Efficiency and effectiveness of Er: YAG laser on carious tissue removal

Research paper thumbnail of Assessing microleakage in resin composite restorations rebonded with a surface sealant and three low-viscosity resin systems

Quintessence international (Berlin, Germany : 1985), 2002

The aim of this study was to assess the ability of three low-viscosity resin systems (Unibond, Si... more The aim of this study was to assess the ability of three low-viscosity resin systems (Unibond, Single Bond, and Fluorseal), used as rebonding agents, and a specific surface-penetrating sealant (Fortify) to prevent microleakage in Class V resin composite restorations. Fifty Class V cavities with the occlusal margin in enamel and the cervical margin in dentin and cementum were prepared on both the buccal and lingual surfaces of sound extracted premolars and restored with a hybrid light-cured resin composite. After finishing and polishing, restorations were randomly assigned to one of five equal groups (n = 10): a control, without surface sealing, and four experimental groups in which margins were etched and rebonded. Specimens were thermocycled, immersed in a 50% silver nitrate solution, sectioned longitudinally, and analyzed for leakage at the occlusal and cervical interfaces. Statistical analysis showed significantly less leakage at the enamel margins for all groups. Fortify and Uni...

Research paper thumbnail of Mechanical properties and bond strength of glass-ionomer cements

The journal of adhesive dentistry, 2002

The objective of this study was to evaluate the mechanical properties and bond strength of glass-... more The objective of this study was to evaluate the mechanical properties and bond strength of glass-ionomer cements (GICs) and resin-modified GICs (RM-GICs) that are indicated as restorative materials for the Atraumatic Restorative Treatment (ART) technique. Fifteen disk specimens for the diametral tensile strength (DTS) test and fifteen cylindrical specimens for the compressive strength (CS) test were made of each GIC: Ketac-Fil, Ketac-Molar (ESPE), Fuji IX and Fuji PLUS (GC). Forty human molars were sectioned and embedded in resin with either buccal or lingual surfaces exposed for the tensile bond strength (TBS) test. The surface was ground until a flattened area of enamel or dentin was obtained. After conditioning, inverted truncated cones of GICs were prepared on the flat tooth surfaces. The powder:liquid ratio of Fuji PLUS was adjusted for restorative purposes. Prior to testing, specimens were stored for 24 h (TBS test) and for 1 h, 24 h, and 7 days (CS and DTS tests) in deionized...

Research paper thumbnail of Avaliação quantitativain vitro'da microinfiltração marginal em restaurações Classe V em resina composta, utilizando-se sistemas adesivos total e self-etching; …

Rev. paul. odontol, 2004

Resumo: O objetivo do presente estudo foi avaliar quantitativamente in vitro a microinfiltração m... more Resumo: O objetivo do presente estudo foi avaliar quantitativamente in vitro a microinfiltração marginal em restaurações classe V em resina composta, utilizando-se diferentes sistemas adesivos: Clearfill SE Bond (Kuraray), One-up bond F (Toruyama) e ...

Research paper thumbnail of Bonding performance of different adhesive systems to deproteinized dentin: Microtensile bond strength and scanning electron microscopy

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005

Deproteinization has been shown to optimize dentin bonding, but differences in adhesive compositi... more Deproteinization has been shown to optimize dentin bonding, but differences in adhesive composition should be considered. The objective of this study was to evaluate the effect of dentin deproteinization on microtensile bond strength (microTBS) of four total-etch adhesive systems (Single Bond/SB, Prime &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp; Bond NT/PB, One Coat Bond/OC, and PQ1/PQ). The ultrastructure of the resin-dentin interfaces was also examined using scanning electron microscopy. Tukey&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s multiple-comparison tests indicated that PB and PQ produced significantly higher microTBS (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.05) after dentin deproteinization (PB=61.53 MPa, PQ=58.18 MPa). This treatment provided statistically lower results for SB (39.08 MPa), but the microTBS of OC to dentin was unaffected by dentin deproteinization. The bonding performance on deproteinized dentin surfaces depended on the characteristics of each adhesive system, as well as the adhesive dentin specificity to the oxidant effect of sodium hypochlorite. Incorporation of fillers in the adhesive, a possible self-etching action, and the presence of a volatile solvent (acetone) were the main factors for a better union between the adhesive system and deproteinized substrate.

Research paper thumbnail of Clareamento caseiro de dentes vitais; Home vital bleaching

Research paper thumbnail of Avaliação da eficiência de desgaste de pontas diamantadas

O objetivo do presente estudo foi avaliar a eficiencia de desgaste sobre dentes bovinos de pontas... more O objetivo do presente estudo foi avaliar a eficiencia de desgaste sobre dentes bovinos de pontas diamantada de diferentes marcas comerciais (KG Sorensen, Microdont, Fava e SSWhite). Para tal finalidade, foram testadas 10 pontas de cada marca comercial, cujo substrato para o desgaste constituia-se de incisivos bovinos, seccionados em 4 partes iguais (2 cervicais e 2 incisais) e incluidos em resina de poliester, totalizando 4 especimes para cada instrumento rotatorio. Para padronizacao do desgaste, foi utilizado um dispositivo, no qual acoplou-se a caneta de alta-rotacao. Todos os testes foram executados sob refrigeracao constante e as pontas foram divididas aleatoriamente e sem identificacao. Os dados foram analisados atraves da analise de variância a dois criterios (p>0,01) e teste de Tukey (p>0,05). Pode-se observar que as pontas diamantadas KG Sorensen demonstraram estatisticamente maior eficiencia no desgaste do que as demais pontas testadas, sendo mais evidenciado apos o 2° seccao de dente. Atraves dos resultados obtidos, pode-se concluir que as pontas diamantadas KG apresentaram melhor desempenho tanto na porcao incisal quanto na cervical. Termos de Indexacao: pontas diamantadas; eficiencia de desgaste; estrutura denta.

Research paper thumbnail of Análise quantitativa in vitro da microinfiltração marginal em restaurações classe V com materiais restauradores adesivos. Influência do uso do laser de Er: YAG no preparo cavitário

Research paper thumbnail of Physical and mechanical properties of a dental resin adhesive containing hydrophobic chitin nanocrystals

Research paper thumbnail of Changes in enamel after bleaching pre-treatment with non-thermal atmospheric plasma

Clinical Plasma Medicine

Abstract The aim of this study was to evaluate color change, mineral composition and topography o... more Abstract The aim of this study was to evaluate color change, mineral composition and topography of the enamel after the pre-treatment with non-thermal atmospheric plasma (NTAP) followed by 38% hydrogen peroxide (HP) bleaching. Buccal enamel of bovine incisors teeth was stained with black tea solution and then divided into five groups (n = 12), according to the plasma application time: PL1 (one minute) or PL2 (2 min) and number of HP applications: HP1 (one) and HP3 (three). The following groups were investigated: HP3, PL1+HP1, PL1+HP3, PL2+HP1 and PL2+HP3. Four color measurements: initial (untreated), after staining and after bleaching (first and second sessions), were performed using an intraoral spectrophotometer (Vita Easyshade). Energy Dispersive X-ray Spectroscopy (EDX) was used to analyze the mineral composition of the enamel before and after two bleaching sessions. Laser confocal microscopy was used to analyze the enamel topography. The pre-treatment with NTAP did not improve the whitening results. The use of NTAP preserves the concentration of calcium and phosphorus, only HP3 group experimented a significant decrease in the mineral concentration. All the bleached groups, with or without NTAP treatment increased their enamel roughness. The application of NTAP did not influence the color change, but it was important to maintain the mineral composition of bleached teeth. All the groups increased their roughness level.

Research paper thumbnail of Laser managed in the treatment of hypomineralized occlusal defects in teeth enamel affected by molar-incisor hypomineralization: A randomized controlled clinical study / Uso do laser no tratamento dos defeitos oclusais hipomineralizados em esmalte de dentes afetados pela hipomineralização molar-i...

Brazilian Journal of Health Review

Background: Nowadays, observed a growing rate of teeth affected by molar incisor hypomineralizati... more Background: Nowadays, observed a growing rate of teeth affected by molar incisor hypomineralization (MIH). These teeth can present persistent inflammation of the pulp, increasing the dentin hypersensitivity and consequently, directly affecting the cleaning of the affected tooth and contributing to the progression of caries lesions. Aims: This study aims to compare the use of diode laser (DL) irradiation (970nm) with the application of a glass ionomer-based sealant (GIC) for the most clinically and cost-effective strategy for the management of first permanent molars affected with MIH. Methods: This is a two-arm, parallel-group, patient randomized superiority-controlled trial, children present MIH molars will be selected and treated either with the application of DL or with the application of GIC-based sealants. Baseline and follow-up evaluations will be assessed through clinical examination and analysis of dental impressions that will be carried out of the affected teeth. The outcome...

Research paper thumbnail of Effect of manual and electrical brushing on the enamel of sound primary teeth and teeth with induced white spot lesions

American journal of dentistry, 2020

PURPOSE To evaluate the effect of different electrical brushing systems on the surface roughness ... more PURPOSE To evaluate the effect of different electrical brushing systems on the surface roughness and wear profile of the enamel of sound primary teeth and teeth with induced white spot lesions. METHODS 45 specimens were obtained from sound primary incisors, and the buccal surface was divided into four parts: sound enamel; enamel with white spot lesions; sound enamel with brushing; and enamel with white spot lesions and brushing. Specimens were randomly divided into three groups (n =15), according to the different brushing systems: Group 1 - Electric rotating toothbrush (Kid's Power Toothbrush - Oral B); Group 2 - Sonic electric toothbrush (Baby Sonic Toothbrush); and Group 3 - Manual toothbrush (Curaprox infantil) (control). The specimens were analyzed for surface roughness and wear profile. Data were analyzed by appropriate statistical tests, with a significance level of 5%. RESULTS Regarding the surface roughness, no significant difference was observed between the groups. Howe...

Research paper thumbnail of Radiotherapy Activates Matrix Metalloproteinases in the Dentinoenamel Junction of Primary Teeth

Journal of dentistry for children, 2020

Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel ... more Purpose: Radiation-related caries is characterized by enamel delamination near the dentinoenamel junction (DEJ). We investigated the activity and expression of the matrix metalloproteinases (MMPs) -2 and -9 in order to understand disease pathogenesis in teeth submitted or not to radiotherapy (RT). Methods: In situ zymography and immunofluorescence assays were performed to evaluate the activity and expression of MMPs -2 and -9, respectively. Twelve primary second molars were randomly assigned into two experimental subgroups: irradiated and nonirradiated. Dental fragments were exposed to radiation at a dose fraction of two Gy for five consecutive days until reaching the total dose of 60 Gy. The percentage of fluorescence in the DEJ was evaluated in three distinct regions of the tooth (cervical, cusp, and pit). The regions were photographed under fluorescence microscopy at 1.25× and 5× magnification. Results: The intensity of fluorescence per mm 2 in the DEJ was higher in the cervical ...

Research paper thumbnail of Influence of Er,Cr:YSGG laser on dentin acid resistance after erosive challenge

American journal of dentistry, 2019

PURPOSE To evaluate the influence of the Er,Cr:YSGG laser with or without the 5% fluoride varnish... more PURPOSE To evaluate the influence of the Er,Cr:YSGG laser with or without the 5% fluoride varnish on the acid resistance of dentin after erosive challenge. METHODS 36 incisors were selected and sectioned, obtaining 72 specimens of 4 mm × 4 mm and randomly divided into eight groups (n = 9). In G1: application of Er,Cr:YSGG (0.1W; 5Hz, air 55%); G2: laser (0.25W; 5Hz, air 55%); G3: fluoride varnish + laser (0.1W; 5Hz, air 55%); G4: fluoride varnish + laser (0.25W, 5Hz, air 55%); G5: fluoride varnish + laser (0.1W; 5Hz, without air); G6: fluoride varnish + laser (0.25W, 5Hz, without air); G7: fluoride varnish and G8: no treatment. When used, the laser was irradiated without water cooling, scanning mode during 10 seconds. The surface roughness data were subjected to ANOVA. For wear profile, we used Kruskal-Wallis test and Dunn post-hoc, all with α= 0.05. RESULTS The results showed no statistically significant difference when comparing the groups as regards to the surface roughness (P&gt...

Research paper thumbnail of Effects of prolonged use of over‐the‐counter bleaching agents on enamel: An in vitro study

Microscopy Research and Technique, 2021

This study evaluated the effects of four over-the-counter (OTC) bleaching products on the propert... more This study evaluated the effects of four over-the-counter (OTC) bleaching products on the properties of enamel. Extracted human molars were randomly assigned into four groups (n = 5): PD: Poladay (SDI), WG: White Teeth Global (White Teeth Global), CW: Crest3DWhite (Procter & Gamble), and HS: HiSmile (HiSmile). The hydrogen peroxide (H2 O2 ) content in each product was analyzed via titration. Twenty teeth were sectioned into quarters, embedded in epoxy resin, and polished. Each quarter-tooth surface was treated with one of the four beaching times: T0: control/no-bleaching, T14: 14 days, T28: 28 days, and T56: 56 days. Materials were applied to enamel surfaces as recommended. Enamel surfaces were examined for ultramicrohardness (UMH), elastic modulus (EM), superficial roughness (Sa), and scanning electron microscopy (SEM). Ten additional teeth were used to evaluate color and degree of demineralization (DD) (n = 5). Data were statistically tested by two-way ANOVA and Tukey's tests (α = 5%). Enamel surfaces treated with PD and WG presented UMH values significantly lower than the controls (p < .05). Elastic modulus (E) was significantly reduced at T14 and T28 for PD, and at T14 for HS (p < .05). A significant increase in Sa was observed for CW at T14 (p < .05). Color changes were observed in the PD and WG groups. Additionally, DD analysis showed significant demineralization at T56 for CW. Overall, more evident morphological alterations were observed for bleaching products with higher concentrations of H2 O2 (p < .05), PD, and WG. Over-the-counter bleaching products containing H2 O2 can significantly alter enamel properties, especially when application time is extended.

Research paper thumbnail of Effects of aging on shear bond strength to dentin and mechanical properties of restorative glass ionomer cements

International Journal of Adhesion and Adhesives, 2020

Abstract Objectives To evaluate the shear bond strength to dentin (SBS), flexural strength (FS), ... more Abstract Objectives To evaluate the shear bond strength to dentin (SBS), flexural strength (FS), and diametral tensile strength (DTS) of four restorative glass ionomer cements after aging in artificial saliva. Materials and methods For SBS testing, sound molars were ground to flat occlusal dentin and fixed with acrylic resin. Teeth were randomly divided into four groups along with the following materials: Ketac Nano, Ketac Molar, Fuji II LC and Equia. Glass ionomer cylinders were bonded to each dentin surface. For testing the mechanical properties, FS bars (25 mm × 2 mm x 2 mm) and DTS discs (4 mm × 2 mm) were fabricated. All specimens were stored in artificial saliva (37 °C) and tested after 24 h and 6 months. Data was analyzed with two-way ANOVA and post-hoc Tukey's tests (α = 5%). Results The SBS results were statistically similar among the four glass ionomer cements (p > 0.05). No significant differences in SBS after 24-h and 6-month storage were found, except for in the Ketac Nano group, where a significant decrease in SBS was found after aging (p Conclusions Aging for 6 months did not affect the SBS of restorative glass ionomer cements, except in the case of Ketac Nano. All glass ionomer cements showed increased FS over time. The DTS remained unchanged – except for the Fuji II LC, which experienced increased DTS.