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Papers by Remigijus Daubaras

Research paper thumbnail of Determining of genetic diversity and genetic relationships among Lithuanian selections of Actinidia kolomikta

The assessing of Lithuanian cultivars and clones of Actinidia kolomikta DNA by RAPD method corrob... more The assessing of Lithuanian cultivars and clones of Actinidia kolomikta DNA by RAPD method corroborated the significant genetic diversity and defined the level of their relationship. Genomic DNA was isolated from fresh leaves following CTAB method. Six decamer oligonucleotide primers Akt-1, Akt-2, Akt-3, 2B, OPA-02 and OPC-02 were used for PCR and a total of 42 scorable fragments yielded. The

Research paper thumbnail of Antioxidant properties, phenolic composition and potentiometric sensor array evaluation of commercial and new blueberry (Vaccinium corymbosum) and bog blueberry (Vaccinium uliginosum) genotypes

Food Chemistry, 2015

Antioxidant properties of juices of newly bred and known blueberry (Vaccinium corymbosum) genotyp... more Antioxidant properties of juices of newly bred and known blueberry (Vaccinium corymbosum) genotypes and wild bog blueberry (Vaccinium uliginosum) were evaluated by ABTS Å+ scavenging capacity (RSC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total phenolic content (TPC) and total anthocyanin content (TAC) assays. TPC varied in the range of 0.85-2.81 mg gallic acid equiv./mL, RSC, FRAP and ORAC values were 6.38-20.9, 3.07-17.8 and 4.21-45.68 lmol Trolox equiv./g, respectively. New blueberry genotypes and bog blueberry demonstrated stronger antioxidant properties and TAC than other studied genotypes. The content of quinic (203-3614 lg/mL), chlorogenic (20.0-346.8 lg/mL) acids and rutin (0.00-26.88 lg/mL) measured by UPLC/ESI-QTOF-MS varied depending on the genotype. Juices were evaluated by electronic tongue; PCA score plot showed that the method discriminates different genotypes although some juice samples were located very closely and overlapping. Significant differences were observed between L ⁄ , a ⁄ , b ⁄ colour parameters of some genotypes.

Research paper thumbnail of Evaluation of the Biochemical Components and Chromatic Properties of the Juice of Vaccinium macrocarpon Aiton and Vaccinium oxycoccos L

Plant Foods for Human Nutrition, 2011

Benzoic acid, total anthocyanins, soluble solids, titratable acidity, and colour properties in ju... more Benzoic acid, total anthocyanins, soluble solids, titratable acidity, and colour properties in juice of the American cranberry Vaccinium macrocarpon and the European cranberry Vaccinium oxycoccos were investigated. Berry juices of V. macrocarpon cultivars were distinguished by their higher total anthocyanin and benzoic acid amounts. These cultivars accumulated on average 43.11 mg/l of benzoic acid and 92.45 mg/l of total anthocyanins. The levels of benzoic acid and total anthocyanins in V. oxycoccos cultivars were 17.52 mg/l and 42.54 mg/l, respectively. The V. macrocarpon cultivars 'Franklin', 'Le Munyon', 'Searles', and 'Early Richard' were selected as the best according to the enhanced total anthocyanins and benzoic acid amounts. The separation of anthocyanins by HPLC-UV-VIS revealed the presence of six anthocyanins, with peonidin-3-galactoside being the most prevalent. Galactoside together with glucoside conjugates comprised the largest percentage of total anthocyanins in the juices of V. macrocarpon and V. oxycoccos cultivars.

Research paper thumbnail of Biochemical and agro-biological diversity of Viburnum opulus genotypes

Central European Journal of Biology, 2010

Interest in the biochemical composition of Viburnum opulus fruit has intensified due to the food ... more Interest in the biochemical composition of Viburnum opulus fruit has intensified due to the food industry's demand for natural vitamins, pigments and other substances that enhance the value of different foods. The present study was conducted to determine the agro-biological and biochemical variability of V. opulus and to select the genotypes that could best serve as sources of health promoting substances. Twelve selected genotypes were evaluated. 'Leningradskaya Otbornaya', V. opulus var. americanum, 'Zarnitsa', and local clone P2 were determined to be the best genotypes for growth in commercial plantations. Fruits of the local clone P3 were characterised by large amounts of total phenolics, ascorbic acid, and reducing sugars. V. opulus var. sargentii and V. opulus var. americanum contained exceptionally large amounts of total phenolics, 1460.0 and 1400.0 mg/100 g, respectively. The amount of ascorbic acid varied from 12.4 to 41.4 mg/100 g, the amount of carotenoids varied from 1.4 to 2.8 mg/100 g, the amount of anthocyanins varied from 23.2 to 44.6 mg/100 g, and the amount of total phenolics varied from 753.0 to 1460.0 mg/100 g. The presence of these large amounts of biologically active compounds enables their use as potent antioxidants. The data describing agro-biological characteristics, biochemical components, and health promoting activities of V. opulus fruits will increase the understanding of this plant and facilitate its use in the food and pharmaceuticals industry.

Research paper thumbnail of Genetic Structure of Isolated Vaccinium oxycoccus Populations in Lithuania

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., 2009

Research paper thumbnail of Evaluation of productivity and biochemical components in fruit of different <i>Viburnum</i> accessions

Biologija, 2008

Accessions of the genus Viburnum with edible fruits were investigated in the field collection of ... more Accessions of the genus Viburnum with edible fruits were investigated in the field collection of the Kaunas Botanical Garden of VMU and those most valuable were selected. Three accessions of V. opulus and the species V. trilobum were distinguished for productivity and raceme size. Biochemical examination of fruit revealed a considerable variation among the accessions in regarding the content of ascorbic acid, anthocyanins, phenolic compounds and carotenoids. V. trilobum and V. sargentii fruits should be appreciated as a source of valuable biochemical components, phenolic in particular.

Research paper thumbnail of Morphological and genetic diversity of European cranberry (Vaccinium oxycoccos L., Ericaceae) clones in Lithuanian reserves

Acta Societatis Botanicorum Poloniae, 2013

Research paper thumbnail of Determining of genetic diversity and genetic relationships among Lithuanian selections of Actinidia kolomikta

The assessing of Lithuanian cultivars and clones of Actinidia kolomikta DNA by RAPD method corrob... more The assessing of Lithuanian cultivars and clones of Actinidia kolomikta DNA by RAPD method corroborated the significant genetic diversity and defined the level of their relationship. Genomic DNA was isolated from fresh leaves following CTAB method. Six decamer oligonucleotide primers Akt-1, Akt-2, Akt-3, 2B, OPA-02 and OPC-02 were used for PCR and a total of 42 scorable fragments yielded. The

Research paper thumbnail of Antioxidant properties, phenolic composition and potentiometric sensor array evaluation of commercial and new blueberry (Vaccinium corymbosum) and bog blueberry (Vaccinium uliginosum) genotypes

Food Chemistry, 2015

Antioxidant properties of juices of newly bred and known blueberry (Vaccinium corymbosum) genotyp... more Antioxidant properties of juices of newly bred and known blueberry (Vaccinium corymbosum) genotypes and wild bog blueberry (Vaccinium uliginosum) were evaluated by ABTS Å+ scavenging capacity (RSC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total phenolic content (TPC) and total anthocyanin content (TAC) assays. TPC varied in the range of 0.85-2.81 mg gallic acid equiv./mL, RSC, FRAP and ORAC values were 6.38-20.9, 3.07-17.8 and 4.21-45.68 lmol Trolox equiv./g, respectively. New blueberry genotypes and bog blueberry demonstrated stronger antioxidant properties and TAC than other studied genotypes. The content of quinic (203-3614 lg/mL), chlorogenic (20.0-346.8 lg/mL) acids and rutin (0.00-26.88 lg/mL) measured by UPLC/ESI-QTOF-MS varied depending on the genotype. Juices were evaluated by electronic tongue; PCA score plot showed that the method discriminates different genotypes although some juice samples were located very closely and overlapping. Significant differences were observed between L ⁄ , a ⁄ , b ⁄ colour parameters of some genotypes.

Research paper thumbnail of Evaluation of the Biochemical Components and Chromatic Properties of the Juice of Vaccinium macrocarpon Aiton and Vaccinium oxycoccos L

Plant Foods for Human Nutrition, 2011

Benzoic acid, total anthocyanins, soluble solids, titratable acidity, and colour properties in ju... more Benzoic acid, total anthocyanins, soluble solids, titratable acidity, and colour properties in juice of the American cranberry Vaccinium macrocarpon and the European cranberry Vaccinium oxycoccos were investigated. Berry juices of V. macrocarpon cultivars were distinguished by their higher total anthocyanin and benzoic acid amounts. These cultivars accumulated on average 43.11 mg/l of benzoic acid and 92.45 mg/l of total anthocyanins. The levels of benzoic acid and total anthocyanins in V. oxycoccos cultivars were 17.52 mg/l and 42.54 mg/l, respectively. The V. macrocarpon cultivars 'Franklin', 'Le Munyon', 'Searles', and 'Early Richard' were selected as the best according to the enhanced total anthocyanins and benzoic acid amounts. The separation of anthocyanins by HPLC-UV-VIS revealed the presence of six anthocyanins, with peonidin-3-galactoside being the most prevalent. Galactoside together with glucoside conjugates comprised the largest percentage of total anthocyanins in the juices of V. macrocarpon and V. oxycoccos cultivars.

Research paper thumbnail of Biochemical and agro-biological diversity of Viburnum opulus genotypes

Central European Journal of Biology, 2010

Interest in the biochemical composition of Viburnum opulus fruit has intensified due to the food ... more Interest in the biochemical composition of Viburnum opulus fruit has intensified due to the food industry's demand for natural vitamins, pigments and other substances that enhance the value of different foods. The present study was conducted to determine the agro-biological and biochemical variability of V. opulus and to select the genotypes that could best serve as sources of health promoting substances. Twelve selected genotypes were evaluated. 'Leningradskaya Otbornaya', V. opulus var. americanum, 'Zarnitsa', and local clone P2 were determined to be the best genotypes for growth in commercial plantations. Fruits of the local clone P3 were characterised by large amounts of total phenolics, ascorbic acid, and reducing sugars. V. opulus var. sargentii and V. opulus var. americanum contained exceptionally large amounts of total phenolics, 1460.0 and 1400.0 mg/100 g, respectively. The amount of ascorbic acid varied from 12.4 to 41.4 mg/100 g, the amount of carotenoids varied from 1.4 to 2.8 mg/100 g, the amount of anthocyanins varied from 23.2 to 44.6 mg/100 g, and the amount of total phenolics varied from 753.0 to 1460.0 mg/100 g. The presence of these large amounts of biologically active compounds enables their use as potent antioxidants. The data describing agro-biological characteristics, biochemical components, and health promoting activities of V. opulus fruits will increase the understanding of this plant and facilitate its use in the food and pharmaceuticals industry.

Research paper thumbnail of Genetic Structure of Isolated Vaccinium oxycoccus Populations in Lithuania

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., 2009

Research paper thumbnail of Evaluation of productivity and biochemical components in fruit of different <i>Viburnum</i> accessions

Biologija, 2008

Accessions of the genus Viburnum with edible fruits were investigated in the field collection of ... more Accessions of the genus Viburnum with edible fruits were investigated in the field collection of the Kaunas Botanical Garden of VMU and those most valuable were selected. Three accessions of V. opulus and the species V. trilobum were distinguished for productivity and raceme size. Biochemical examination of fruit revealed a considerable variation among the accessions in regarding the content of ascorbic acid, anthocyanins, phenolic compounds and carotenoids. V. trilobum and V. sargentii fruits should be appreciated as a source of valuable biochemical components, phenolic in particular.

Research paper thumbnail of Morphological and genetic diversity of European cranberry (Vaccinium oxycoccos L., Ericaceae) clones in Lithuanian reserves

Acta Societatis Botanicorum Poloniae, 2013