Renata Franzon - Academia.edu (original) (raw)
Papers by Renata Franzon
Revista da Faculdade de Odontologia de Porto Alegre
Objetivo: descrever o tipo de aleitamento materno, a idade em que ocorreu a introdução alimentar,... more Objetivo: descrever o tipo de aleitamento materno, a idade em que ocorreu a introdução alimentar, as características da instalação dos hábitos orais e a ocorrência das más oclusões em crianças de três a cinco anos. Materiais e métodos: estudo transversal realizado com 93 crianças por meio da aplicação de questionário aos responsáveis acerca do aleitamento materno, introdução alimentar e hábitos orais. Foi realizado exame clínico odontológico para avaliação da oclusão dentária. O nível de significância utilizado foi de 5%. Resultados: 93,5% das crianças foram amamentados no seio materno, destas, 6,4% foram amamentadas exclusivamente por mais de 6 meses. A mediana de idade da introdução da água e chá foi de cinco meses. O uso da mamadeira foi observado em 86% das crianças e a utilização da chupeta em 49,5%. A associação entre o tempo de aleitamento materno exclusivo e a presença de hábitos orais foi significativa (p=0,035). Crianças com o hábito de ficar com a boca aberta durante a no...
The purpose of this study was to investigate dentin rehardening in the remaining carious dentin a... more The purpose of this study was to investigate dentin rehardening in the remaining carious dentin after indirect pulp treatment (IPT) using microhardness analysis after 37 to 71 months. Eighteen teeth submitted to IPT and capped with calcium hydroxide (CH) or gutta-percha (GP) were evaluated (treated group). Ten sound molars and 10 molars with deep acute carious lesions were selected to serve as positive and negative control groups, respectively. In the treated group, restorations and pulp-capping materials were removed. In the positive control group, 3- to 4-mm deep cavities were prepared. In the negative control group, the carious tissue was removed. Microhardness analysis was performed at 10-, 35-, 60-, 85-, and 110-microm depths. Data were analyzed using 1-way analysis of variance (P<.05). Microhardness values for sound, carious, and treated groups at 10-, 35-, 60-, 85-, and 110-microm depths showed a statistically significant difference (P<or=.01) among the groups for microhardness. No difference was observed between CH- and GP-treated groups for microhardness. The results showed a hardness increase in treated teeth when compared to carious teeth in all dentin depths investigated, suggesting mineral gain after treatment.
Metabolic brain disease, 2002
Homocystinuria is a metabolic disorder caused by deficiency of cystathione beta-synthase activity... more Homocystinuria is a metabolic disorder caused by deficiency of cystathione beta-synthase activity leading to tissue accumulation of homocysteine (Hcy); affected patients present neurological dysfunction. Considering that Hcy induces free radical formation and that memory is impaired by oxidative stress, in the present study we investigated the effect of an acute administration of Hcy on retrieval of step-down inhibitory avoidance in adult rats. The action of vitamins E and C on the effects produced by Hcy was also tested. Adult Wistar rats were pretreated for 1 week with daily i.p. administration of saline (control group) and vitamins E and C (vitamin E 40 mg/kg and vitamin C 100 mg/kg). Hcy (11 mmol/kg) or an equivalent volume of 0.9% saline were administered 1 h before training, 1 h before testing, or immediately after training sessions. Memory was significantly impaired in Hcy-treated group, whereas the rats chronically treated with vitamins E and C had this effect prevented. Pre...
Although several advances have occurred concerning the use of electroconvulsive therapy, little p... more Although several advances have occurred concerning the use of electroconvulsive therapy, little progress has been made in understanding the mechanisms underlying its therapeutic or side effects. Na + ,K + -ATPase is an important enzyme of central nervous system, responsible for ionic gradient maintenance and consumption of approximately 40-50% of brain ATP. This work was performed in order to determine Na + ,K + -ATPase activity after acute and chronic electroconvulsive shock. Results showed an inhibition of Na + ,K + -ATPase activity in the hippocampus 48 h, 7, 30, 60 and 90 days after a single electroconvulsive shock. Chronic treatment diminished the enzyme activity in the hippocampus 7 and 30 days after electroconvulsive (ECS) sessions. Our findings demonstrated that Na + ,K + -ATPase activity is altered by ECS.
Guanidinoacetate methyltransferase deficiency (GAMT-deficiency) is an inherited neurometabolic di... more Guanidinoacetate methyltransferase deficiency (GAMT-deficiency) is an inherited neurometabolic disorder clinically characterized by epilepsy and mental retardation and biochemically by accumulation of guanidinoacetate (GAA) and depletion of creatine. Although the neurological symptoms are predominant, the pathogenesis of the brain dysfunction in this disorder is not yet established. In the present study we investigated the in vitro effect of GAA on Na + , K + -ATPase and Mg 2+ -ATPase activities in synaptic plasma membrane from hippocampus of young rats. Results showed that GAA significantly inhibited Na + , K + -ATPase activity without affecting Mg 2+ -ATPase activity. We also evaluated the effect of glutathione (GSH), trolox, N -nitro-l-arginine methyl ester (L-NAME) and taurine (Tau) on the inhibition elicited by GAA on Na + , K + -ATPase activity. GSH, trolox, L-NAME and Tau per se did not alter Na + , K + -ATPase activity. However, L-NAME and taurine prevented the inhibitory effect of GAA on this enzyme activity. Our findings suggest that the inhibition of Na + , K + -ATPase activity caused by GAA is possibly mediated by nitric oxide (NO) formation and/or synaptic membrane alteration. The present data may contribute to the understanding of the neurological dysfunction characteristic of GAMT-deficient patients.
Na ϩ ,K ϩ -ATPase and Mg 2ϩ -ATPase activities were determined in the synaptic plasma membranes f... more Na ϩ ,K ϩ -ATPase and Mg 2ϩ -ATPase activities were determined in the synaptic plasma membranes from hippocampus of rats subjected to chronic and acute proline administration. Na ϩ ,K ϩ -ATPase activity was significantly reduced in chronic and acute treatment by 33% and 40%, respectively. Mg 2ϩ -ATPase activity was not altered by any treatment. In another set of experiments, synaptic plasma membranes were prepared from hippocampus and incubated with proline or glutamate at final concentrations ranging from 0.2 to 2.0 mM. Na ϩ ,K ϩ -ATPase, but not Mg 2ϩ -ATPase was inhibited (30%) by the two amino acids. In addition, competition between proline and glutamate for the enzyme activity was observed, suggesting a common binding site for these amino acids. Considering that Na ϩ ,K ϩ -ATPase activity is critical for normal brain function, the results of the present study showing a marked inhibition of this enzyme by proline may be associated with the neurological dysfunction found in patients affected by type II hyperprolinemia.
In the present study we investigate the effect of homocysteine (Hcy) administration, the main met... more In the present study we investigate the effect of homocysteine (Hcy) administration, the main metabolite accumulating in homocystinuria, on butyrylcholinesterase (BuChE) activity in serum of rats. For the acute treatment, 29-day-old Wistar rats received one subcutaneous injection of Hcy (0.6 µmol/g) or saline (control) and were killed 1 h later. For the chronic treatment, Hcy was administered subcutaneously to rats from the 6th to the 28th day of life. Control rats received saline. The rats were killed 12 h after the last injection. In another set of experiments, rats were pretreated for one week with vitamins E and C or saline and 12 h after the last injection received one single injection of Hcy or saline, being killed 1 h later. Serum was used to determine BuChE activity. Our results showed that acute and chronic administration of Hcy significantly decreased BuChE activity. Furthermore, vitamins E and C per se did not alter BuChE activity, but prevented the reduction of this enzyme activity caused by acute administration of Hcy. The data suggest that the inhibitory effect of Hcy on BuChE activity is probably mediated by free radicals, since vitamins E and C administration prevented such effect.
Diabetes research and clinical practice, 2005
The aim of this study was to investigate Na+, K(+)-ATPase activity in cerebral cortex, hippocampu... more The aim of this study was to investigate Na+, K(+)-ATPase activity in cerebral cortex, hippocampus and hypothalamus of diabetic rats. The action of dietary soy protein on the effect produced by diabetes on this activity was also tested. Forty-nine-day-old Wistar were divided into two groups: diabetes streptozotocin (50 mg/kg body weight) and control (citrate solution). Rats were sacrificed 56 days later. In other set of experiments, rats received a dietary with casein (control) from day 21 to the 49 of postnatal-age and were subjected to diabetes or received citrate (control). One week later, rats received a special dietary with soy protein with isoflavones or casein (control) from day 56 to the 105 of postnatal-age. Results showed that diabetic rats presented a reduction ( approximately 40%) of Na+, K(+)-ATPase activity in all structures studied. Pretreatment with soy protein prevented the inhibitory effects of diabetes on the enzyme activity. Assuming the possibility that these ef...
American journal of …, 2007
1. Am J Dent. 2007 Jun;20(3):189-92. Clinical and radiographic evaluation of indirect pulp treatm... more 1. Am J Dent. 2007 Jun;20(3):189-92. Clinical and radiographic evaluation of indirect pulp treatment in primary molars: 36 months follow-up. Franzon R, Casagrande L, Pinto AS, García-Godoy F, Maltz M, de Araujo FB. Department ...
Caries Research, 2014
To compare 24-month pulp health outcomes of partial caries removal (PCR) and total caries removal... more To compare 24-month pulp health outcomes of partial caries removal (PCR) and total caries removal (TCR) with composite restoration in primary molars. 48 children aged 3-8 years with at least one molar with a deep carious lesion were included. 120 teeth were randomized to control (TCR; n = 54; 69% class II) and test (PCR; n = 66; 63% class II) groups. Total absence of carious tissue was confirmed using a blunt-tipped probe in the TCR group. For PCR, excavation was stopped when hardened, dried dentin with a leathery consistency was achieved. Pulpotomy was performed in cases of pulp exposure. Pulp exposure occurred in 2 and 27.5% of teeth treated with PCR and TCR, respectively (p < 0.01). The operative time was significantly higher for TCR than PCR. Success rates were 92 and 96% in the PCR and TCR groups, respectively (p = 0.34). The success rate tended to be lower in occlusoproximal (92%) than in occlusal (100%) lesions (p = 0.08). The clinical and radiographic success rates of PCR and TCR in primary teeth with deep carious lesions were high and did not differ significantly, indicating that PCR is a reliable minimally invasive approach in primary teeth and that the retention of carious dentin does not interfere with pulp vitality. Moreover, PCR provided other clinically relevant advantages over TCR, especially lower incidence of pulp exposure and lower operative time.
Neurobiology of learning …, 2005
This study was undertaken to verify the effects of chronic stress and lithium treatments on the h... more This study was undertaken to verify the effects of chronic stress and lithium treatments on the hippocampal Na + ,K + -ATPase activity of rats, as well as to investigate the effects of stress interruption and post-stress lithium treatment on this enzyme activity and on spatial memory. Two ...
Neurochemical …, 2001
The objective of the present study was to investigate the effects of preincubation of hippocampus... more The objective of the present study was to investigate the effects of preincubation of hippocampus homogenates in the presence of homocysteine or methionine on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes of rats. Homocysteine significantly inhibited Na+, K+-ATPase activity, whereas methionine had no effect. Mg2+-ATPase activity was not altered by the metabolites. We also evaluated the effect of incubating glutathione, cysteine, dithiothreitol, trolox, superoxide dismutase and GM1 ganglioside alone or incubation with homocysteine on Na+, K+-ATPase activity. Tested compounds did not alter Na+, K+-ATPase and Mg2+-ATPase activities, but except for trolox, prevented the inhibitory effect of homocysteine on Na+, K+-ATPase activity. These results suggest that inhibition of this enzyme activity by homocysteine is possibly mediated by free radicals and may contribute to the neurological dysfunction found in homocystinuric patients.
International Journal of …, 2003
In the present study, we investigated the effect of Vitamins E and C on the inhibition of Na + ,K... more In the present study, we investigated the effect of Vitamins E and C on the inhibition of Na + ,K + -ATPase activity provoked by proline (Pro) administration in rat hippocampus. Five-day-old rats were pretreated for 1 week with daily ip administration of saline (control) or Vitamin E ( ...
American journal of dentistry, 2012
To clinically evaluate the status of caries lesions adjacent to restorations (AdjCL) in primary m... more To clinically evaluate the status of caries lesions adjacent to restorations (AdjCL) in primary molars, and its relationship to the child's carious activity and marginal restoration integrity. Three independent examiners (trained, calibrated and blinded) evaluated 64 randomly selected restorations (occlusal and occluso-proximal) by the AdjCL status (kappa = 0.844), the restoration marginal integrity (kappa = 1) and the radiographic presence of lesions in the occlusal restoration margins (kappa = 1). One of the examiners also evaluated the child's carious activity (kappa = 1). The variables were related to the outcome through Chi-square and Fisher's Exact analysis (alpha = 5%). A prevalence of 40.63% AdjCL (88.46% inactive) was seen, with no significant association to the child's carious activity (P = 0.23). The association of the absence of AdjCL and the presence of marginal integrity was statistically significant (P = 0.013). Also, the presence of AdjCL occurred esp...
Revista da Faculdade de Odontologia de Porto Alegre
Objetivo: descrever o tipo de aleitamento materno, a idade em que ocorreu a introdução alimentar,... more Objetivo: descrever o tipo de aleitamento materno, a idade em que ocorreu a introdução alimentar, as características da instalação dos hábitos orais e a ocorrência das más oclusões em crianças de três a cinco anos. Materiais e métodos: estudo transversal realizado com 93 crianças por meio da aplicação de questionário aos responsáveis acerca do aleitamento materno, introdução alimentar e hábitos orais. Foi realizado exame clínico odontológico para avaliação da oclusão dentária. O nível de significância utilizado foi de 5%. Resultados: 93,5% das crianças foram amamentados no seio materno, destas, 6,4% foram amamentadas exclusivamente por mais de 6 meses. A mediana de idade da introdução da água e chá foi de cinco meses. O uso da mamadeira foi observado em 86% das crianças e a utilização da chupeta em 49,5%. A associação entre o tempo de aleitamento materno exclusivo e a presença de hábitos orais foi significativa (p=0,035). Crianças com o hábito de ficar com a boca aberta durante a no...
The purpose of this study was to investigate dentin rehardening in the remaining carious dentin a... more The purpose of this study was to investigate dentin rehardening in the remaining carious dentin after indirect pulp treatment (IPT) using microhardness analysis after 37 to 71 months. Eighteen teeth submitted to IPT and capped with calcium hydroxide (CH) or gutta-percha (GP) were evaluated (treated group). Ten sound molars and 10 molars with deep acute carious lesions were selected to serve as positive and negative control groups, respectively. In the treated group, restorations and pulp-capping materials were removed. In the positive control group, 3- to 4-mm deep cavities were prepared. In the negative control group, the carious tissue was removed. Microhardness analysis was performed at 10-, 35-, 60-, 85-, and 110-microm depths. Data were analyzed using 1-way analysis of variance (P<.05). Microhardness values for sound, carious, and treated groups at 10-, 35-, 60-, 85-, and 110-microm depths showed a statistically significant difference (P<or=.01) among the groups for microhardness. No difference was observed between CH- and GP-treated groups for microhardness. The results showed a hardness increase in treated teeth when compared to carious teeth in all dentin depths investigated, suggesting mineral gain after treatment.
Metabolic brain disease, 2002
Homocystinuria is a metabolic disorder caused by deficiency of cystathione beta-synthase activity... more Homocystinuria is a metabolic disorder caused by deficiency of cystathione beta-synthase activity leading to tissue accumulation of homocysteine (Hcy); affected patients present neurological dysfunction. Considering that Hcy induces free radical formation and that memory is impaired by oxidative stress, in the present study we investigated the effect of an acute administration of Hcy on retrieval of step-down inhibitory avoidance in adult rats. The action of vitamins E and C on the effects produced by Hcy was also tested. Adult Wistar rats were pretreated for 1 week with daily i.p. administration of saline (control group) and vitamins E and C (vitamin E 40 mg/kg and vitamin C 100 mg/kg). Hcy (11 mmol/kg) or an equivalent volume of 0.9% saline were administered 1 h before training, 1 h before testing, or immediately after training sessions. Memory was significantly impaired in Hcy-treated group, whereas the rats chronically treated with vitamins E and C had this effect prevented. Pre...
Although several advances have occurred concerning the use of electroconvulsive therapy, little p... more Although several advances have occurred concerning the use of electroconvulsive therapy, little progress has been made in understanding the mechanisms underlying its therapeutic or side effects. Na + ,K + -ATPase is an important enzyme of central nervous system, responsible for ionic gradient maintenance and consumption of approximately 40-50% of brain ATP. This work was performed in order to determine Na + ,K + -ATPase activity after acute and chronic electroconvulsive shock. Results showed an inhibition of Na + ,K + -ATPase activity in the hippocampus 48 h, 7, 30, 60 and 90 days after a single electroconvulsive shock. Chronic treatment diminished the enzyme activity in the hippocampus 7 and 30 days after electroconvulsive (ECS) sessions. Our findings demonstrated that Na + ,K + -ATPase activity is altered by ECS.
Guanidinoacetate methyltransferase deficiency (GAMT-deficiency) is an inherited neurometabolic di... more Guanidinoacetate methyltransferase deficiency (GAMT-deficiency) is an inherited neurometabolic disorder clinically characterized by epilepsy and mental retardation and biochemically by accumulation of guanidinoacetate (GAA) and depletion of creatine. Although the neurological symptoms are predominant, the pathogenesis of the brain dysfunction in this disorder is not yet established. In the present study we investigated the in vitro effect of GAA on Na + , K + -ATPase and Mg 2+ -ATPase activities in synaptic plasma membrane from hippocampus of young rats. Results showed that GAA significantly inhibited Na + , K + -ATPase activity without affecting Mg 2+ -ATPase activity. We also evaluated the effect of glutathione (GSH), trolox, N -nitro-l-arginine methyl ester (L-NAME) and taurine (Tau) on the inhibition elicited by GAA on Na + , K + -ATPase activity. GSH, trolox, L-NAME and Tau per se did not alter Na + , K + -ATPase activity. However, L-NAME and taurine prevented the inhibitory effect of GAA on this enzyme activity. Our findings suggest that the inhibition of Na + , K + -ATPase activity caused by GAA is possibly mediated by nitric oxide (NO) formation and/or synaptic membrane alteration. The present data may contribute to the understanding of the neurological dysfunction characteristic of GAMT-deficient patients.
Na ϩ ,K ϩ -ATPase and Mg 2ϩ -ATPase activities were determined in the synaptic plasma membranes f... more Na ϩ ,K ϩ -ATPase and Mg 2ϩ -ATPase activities were determined in the synaptic plasma membranes from hippocampus of rats subjected to chronic and acute proline administration. Na ϩ ,K ϩ -ATPase activity was significantly reduced in chronic and acute treatment by 33% and 40%, respectively. Mg 2ϩ -ATPase activity was not altered by any treatment. In another set of experiments, synaptic plasma membranes were prepared from hippocampus and incubated with proline or glutamate at final concentrations ranging from 0.2 to 2.0 mM. Na ϩ ,K ϩ -ATPase, but not Mg 2ϩ -ATPase was inhibited (30%) by the two amino acids. In addition, competition between proline and glutamate for the enzyme activity was observed, suggesting a common binding site for these amino acids. Considering that Na ϩ ,K ϩ -ATPase activity is critical for normal brain function, the results of the present study showing a marked inhibition of this enzyme by proline may be associated with the neurological dysfunction found in patients affected by type II hyperprolinemia.
In the present study we investigate the effect of homocysteine (Hcy) administration, the main met... more In the present study we investigate the effect of homocysteine (Hcy) administration, the main metabolite accumulating in homocystinuria, on butyrylcholinesterase (BuChE) activity in serum of rats. For the acute treatment, 29-day-old Wistar rats received one subcutaneous injection of Hcy (0.6 µmol/g) or saline (control) and were killed 1 h later. For the chronic treatment, Hcy was administered subcutaneously to rats from the 6th to the 28th day of life. Control rats received saline. The rats were killed 12 h after the last injection. In another set of experiments, rats were pretreated for one week with vitamins E and C or saline and 12 h after the last injection received one single injection of Hcy or saline, being killed 1 h later. Serum was used to determine BuChE activity. Our results showed that acute and chronic administration of Hcy significantly decreased BuChE activity. Furthermore, vitamins E and C per se did not alter BuChE activity, but prevented the reduction of this enzyme activity caused by acute administration of Hcy. The data suggest that the inhibitory effect of Hcy on BuChE activity is probably mediated by free radicals, since vitamins E and C administration prevented such effect.
Diabetes research and clinical practice, 2005
The aim of this study was to investigate Na+, K(+)-ATPase activity in cerebral cortex, hippocampu... more The aim of this study was to investigate Na+, K(+)-ATPase activity in cerebral cortex, hippocampus and hypothalamus of diabetic rats. The action of dietary soy protein on the effect produced by diabetes on this activity was also tested. Forty-nine-day-old Wistar were divided into two groups: diabetes streptozotocin (50 mg/kg body weight) and control (citrate solution). Rats were sacrificed 56 days later. In other set of experiments, rats received a dietary with casein (control) from day 21 to the 49 of postnatal-age and were subjected to diabetes or received citrate (control). One week later, rats received a special dietary with soy protein with isoflavones or casein (control) from day 56 to the 105 of postnatal-age. Results showed that diabetic rats presented a reduction ( approximately 40%) of Na+, K(+)-ATPase activity in all structures studied. Pretreatment with soy protein prevented the inhibitory effects of diabetes on the enzyme activity. Assuming the possibility that these ef...
American journal of …, 2007
1. Am J Dent. 2007 Jun;20(3):189-92. Clinical and radiographic evaluation of indirect pulp treatm... more 1. Am J Dent. 2007 Jun;20(3):189-92. Clinical and radiographic evaluation of indirect pulp treatment in primary molars: 36 months follow-up. Franzon R, Casagrande L, Pinto AS, García-Godoy F, Maltz M, de Araujo FB. Department ...
Caries Research, 2014
To compare 24-month pulp health outcomes of partial caries removal (PCR) and total caries removal... more To compare 24-month pulp health outcomes of partial caries removal (PCR) and total caries removal (TCR) with composite restoration in primary molars. 48 children aged 3-8 years with at least one molar with a deep carious lesion were included. 120 teeth were randomized to control (TCR; n = 54; 69% class II) and test (PCR; n = 66; 63% class II) groups. Total absence of carious tissue was confirmed using a blunt-tipped probe in the TCR group. For PCR, excavation was stopped when hardened, dried dentin with a leathery consistency was achieved. Pulpotomy was performed in cases of pulp exposure. Pulp exposure occurred in 2 and 27.5% of teeth treated with PCR and TCR, respectively (p < 0.01). The operative time was significantly higher for TCR than PCR. Success rates were 92 and 96% in the PCR and TCR groups, respectively (p = 0.34). The success rate tended to be lower in occlusoproximal (92%) than in occlusal (100%) lesions (p = 0.08). The clinical and radiographic success rates of PCR and TCR in primary teeth with deep carious lesions were high and did not differ significantly, indicating that PCR is a reliable minimally invasive approach in primary teeth and that the retention of carious dentin does not interfere with pulp vitality. Moreover, PCR provided other clinically relevant advantages over TCR, especially lower incidence of pulp exposure and lower operative time.
Neurobiology of learning …, 2005
This study was undertaken to verify the effects of chronic stress and lithium treatments on the h... more This study was undertaken to verify the effects of chronic stress and lithium treatments on the hippocampal Na + ,K + -ATPase activity of rats, as well as to investigate the effects of stress interruption and post-stress lithium treatment on this enzyme activity and on spatial memory. Two ...
Neurochemical …, 2001
The objective of the present study was to investigate the effects of preincubation of hippocampus... more The objective of the present study was to investigate the effects of preincubation of hippocampus homogenates in the presence of homocysteine or methionine on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes of rats. Homocysteine significantly inhibited Na+, K+-ATPase activity, whereas methionine had no effect. Mg2+-ATPase activity was not altered by the metabolites. We also evaluated the effect of incubating glutathione, cysteine, dithiothreitol, trolox, superoxide dismutase and GM1 ganglioside alone or incubation with homocysteine on Na+, K+-ATPase activity. Tested compounds did not alter Na+, K+-ATPase and Mg2+-ATPase activities, but except for trolox, prevented the inhibitory effect of homocysteine on Na+, K+-ATPase activity. These results suggest that inhibition of this enzyme activity by homocysteine is possibly mediated by free radicals and may contribute to the neurological dysfunction found in homocystinuric patients.
International Journal of …, 2003
In the present study, we investigated the effect of Vitamins E and C on the inhibition of Na + ,K... more In the present study, we investigated the effect of Vitamins E and C on the inhibition of Na + ,K + -ATPase activity provoked by proline (Pro) administration in rat hippocampus. Five-day-old rats were pretreated for 1 week with daily ip administration of saline (control) or Vitamin E ( ...
American journal of dentistry, 2012
To clinically evaluate the status of caries lesions adjacent to restorations (AdjCL) in primary m... more To clinically evaluate the status of caries lesions adjacent to restorations (AdjCL) in primary molars, and its relationship to the child's carious activity and marginal restoration integrity. Three independent examiners (trained, calibrated and blinded) evaluated 64 randomly selected restorations (occlusal and occluso-proximal) by the AdjCL status (kappa = 0.844), the restoration marginal integrity (kappa = 1) and the radiographic presence of lesions in the occlusal restoration margins (kappa = 1). One of the examiners also evaluated the child's carious activity (kappa = 1). The variables were related to the outcome through Chi-square and Fisher's Exact analysis (alpha = 5%). A prevalence of 40.63% AdjCL (88.46% inactive) was seen, with no significant association to the child's carious activity (P = 0.23). The association of the absence of AdjCL and the presence of marginal integrity was statistically significant (P = 0.013). Also, the presence of AdjCL occurred esp...