Renata Jurišić Grubešić - Academia.edu (original) (raw)

Papers by Renata Jurišić Grubešić

Plants

Free volatile compounds were isolated from 21 Croatian Veronica species studied by hydrodistillat... more Free volatile compounds were isolated from 21 Croatian Veronica species studied by hydrodistillation (HD) and microwave extraction (ME) and analyzed by gas chromatography coupled with mass spectrometry. Principal Component Analysis (PCA) distinguished some clusters based on the relative proportion of major compounds, such as hexadecanoic acid, hexahydrofarnesyl acetone, phytol, E-caryophyllene, and caryophyllene oxide, which were identified in all species studied by both isolation methods. In addition to these compounds, germacrene D, δ-selinene, and eicosane were also identified in five samples from dry habitats isolated using ME. Allo-aromadendrene and β-ionone are particularly abundant in five species from wet habitats isolated by both methods. The peculiarities of Veronica species from moderate habitats isolated with HD are benzene acetaldehyde, n-nonanal, and the identification of significant compounds from the hydrocarbon class, while the peculiarity of ME is (E)-β-damascenone...

Farmaceutski glasnik, 2016

Phytochemical characterization of bioactive pol yp henolic compounds from endemic species Centaur... more Phytochemical characterization of bioactive pol yp henolic compounds from endemic species Centaurea ragusina L. (Asteraceae) A b s t r a c t-Phytochemical characterization of the Croatian ende rnic species Centaurea ragusintt L. (Asteraceae) was carried out. The pre sence of polyphenols ina bove ground plant parts was proven by using chemi cal reactions ofdeveloping colored products and precipitates. lhe thin layer chromatography showed the presence of quercetin, rutin, and chlorogenic acid in the rnethanolic extract of C. ragusina. Quantitative analysis of totaf po61phenols (TP), tannins (T), fiavonoids (F), and phenolic acids (PA) was carried out using three dif.ferent spectrophotometric methods. 7he contents of ana61zed po6phenolic cornpounds were as follows: 3.04±0.04% (TP), 0.36±0.04% (T), 0.25±0.00% (F), and 0.38±0.01% (PA). 7his phyto chemical characterization is a contribution to the scientific study of Croa tian endemic species and completes the existing knowledge about phytothe rapeutic potential of C. ragusina, especially in relation to the content of bioactive polyphenolic substances.

Acta botanica Croatica, 2020

As a contribution to chemotaxonomic relations, a quantitative analysis of bioactive phenolic comp... more As a contribution to chemotaxonomic relations, a quantitative analysis of bioactive phenolic compounds was carried out for the first time in Drypis spinosa L. subsp. spinosa and D. spinosa subsp. jacquiniana Murb. et Wettst. ex Murb. in Croatia. Total polyphenols (TP), tannins (T) and total flavonoids (TF) were determined in samples of leaves, stems, and roots using UV-Vis spectrophotometric methods. For the subsp. spinosa, the highest content of TF was in leaves (0.09%), as was the highest amount of TP (2.36%) and T (1.12%). In the subsp. jacquiniana, the highest contents of TF (0.10%), TP (1.96%), and T (0.88%) were measured in stems. Coumaric, ferulic and rosmarinic acid were identified and quantified by HPLC analysis in both subspecies. Quercetin and sinapic acid were identified only in subsp. spinosa, while rutin and naringenin were found only in subsp. jacquiniana. Among them, ferulic acid was identified only in flowers of both subspecies. The results of this study represent a...

5. hrvatski kongres farmacije s međunarodnim sudjelovanjem. Farmaceutska izvrsnost u službi zdravlja. Knjiga sažetaka, 2015

Morinda citrifolia L. (noni) pantropski je rasprostranjena biljna vrsta iz porodice Rubiaceae, ci... more Morinda citrifolia L. (noni) pantropski je rasprostranjena biljna vrsta iz porodice Rubiaceae, cija se ljekovita i nutritivna svojstva cijene vec vise od 2000 godina. Prema rezultatima suvremenih znanstvenih istraživanja noni regulira stanicne funkcije, jaca imunoloski sustav, suzbija rast tumora, djeluje protuupalno, antimikrobno, antioksidativno i sedativno, te ublažava bol i snižava visoki krvni tlak. Fitokemijskim istraživanjima utvrđeno je da noni obiluje brojnim bioaktivnim tvarima, kao sto su alkaloidi, antrakinoni, kumarini, polisaharidi, iridoidi, flavonoidi, lignani, terpeni, aminokiseline, masne kiseline, minerali i vitamini. U okviru ovoga rada određen je sadržaj makro- i mikroelemenata u dehidriranoj pulpi i koncentratu soka svježih plodova vrste Morinda citrifolia havajskog podrijetla primjenom metode atomske apsorpcijske spektrofotometrije. Plamenom tehnikom (FAAS) izmjerene su koncentracije Ca, Cu (samo pulpa), Fe, K, Mg, Mn, Na i Zn. Primjenom grafitne tehnike (GFAA...

Sveu ilište u Zagrebu, Farmaceutsko-biokemijski fakultet (FBF), A. Kova i a 1, Zagreb, Hrvatska 1... more Sveu ilište u Zagrebu, Farmaceutsko-biokemijski fakultet (FBF), A. Kova i a 1, Zagreb, Hrvatska 1 Centar za primijenjenu farmaciju, 2 Zavod za farmakologiju, 3 Zavod za farmaceutsku tehnologiju, 4 Zavod za farmakognoziju 5 Zavod za analitiku i kontrolu lijekova

Skripta obrađuje kvalitativne i kvantitativne metode analize farmaceutskih uzoraka prema propisim... more Skripta obrađuje kvalitativne i kvantitativne metode analize farmaceutskih uzoraka prema propisima Europske farmakopeje, a ukljucuje identifikaciju, ispitivanje cistoce ljekovitih i pomocnih tvari, određivanje sadržaja farmaceutskih tvari i njihovih oneciscenja te analizu ljekovitih oblika i validaciju analitickih postupaka.

Skripta obrađuje kvantitativne metode analize farmaceutskih uzoraka prema propisima Europske farm... more Skripta obrađuje kvantitativne metode analize farmaceutskih uzoraka prema propisima Europske farmakopeje, a ukljucuje ispitivanje cistoce ljekovitih i pomocnih tvari, određivanje sadržaja farmaceutskih tvari i njihovih oneciscenja te analizu ljekovitih oblika i validaciju analitickih postupaka.Cistoca farmaceutskih tvari ispituje se UV-Vis i atomskom apsorpcijskom spektrometrijom, tankoslojnom, tekucinskom i plinskom kromatografijom, utvrđivanjem granicnih vrijednosti anorganskih iona i teskih metala, određivanjem fizikalnih konstanti, ispitivanjem obojenosti, bistrine i stupnja zamucenja te kiselosti/lužnatosti otopine. Titrimetrijskim metodama analize, UV-Vis spektrofotometrijom i tekucinskom kromatografijom visoke djelotvornosti određuje se sadržaj ljekovitih i pomocnih tvari.

ln this work qualitative analysis of some bioactive compounds of February Daphne (Daphne mezereum... more ln this work qualitative analysis of some bioactive compounds of February Daphne (Daphne mezereum L.) and Alpine Daphne (D. alpina L.) was performed. The following bioactive compounds were detected using thin-layer chromatography methods (TLC) in methanolic extracts of leaves, stems and roots: flavonoids, coumarins, phenolic acids, saponins, sterols, triterpenes and triterpenic acids. Qualitative analysis of tannins was performed by general reactions of developing coloured products and sediments. Hydrolysable tannins were detected in all examined extracts, while the reaction with condensed tannins was negative for all investigated specimens.

Plants, 2020

Chemical profile and antioxidant activity of the species Veronica saturejoides Vis. ssp. saturejo... more Chemical profile and antioxidant activity of the species Veronica saturejoides Vis. ssp. saturejoides (Plantaginaceae)—which is endemic to Croatia, Bosnia and Herzegovina and Montenegro —were investigated. Volatile compounds produced by glandular trichomes (composed of one stalk cell and two elliptically formed head cells according to scanning electron microscope investigation) were isolated from the plants collected in two locations. Additionally, as a part of specialized metabolites, total polyphenols, total tannins, total flavonoids and total phenolic acids were determined spectrophotometrically. In the lipophilic volatile fractions-essential oils, the most abundant compounds identified were hexahydrofarnesyl acetone, caryophyllene oxide and hexadecanoic acid. In total, the class of oxygenated sesquiterpenes and the group of fatty aldehydes, acids and alcoholic compounds dominated in the essential oils. In the hydrophilic volatile fractions-hydrosols, the most abundant compounds ...

Natural Product Communications, 2019

The aim of this study included the phytochemical screening of extracts and essential oil of Erodi... more The aim of this study included the phytochemical screening of extracts and essential oil of Erodium cicutarium (L.) L’Hér. ex Aiton (Geraniaceae), a native traditional medicinal plant from Croatia, as well as a highly detailed micromorphological characterization of its aerial parts. The contents of total polyphenols and tannins (TP and T), total flavonoids (TF), and total hydroxycinnamic derivatives (THD) in plant extracts were quantified in plant material from 4 localities (Plitvice, Podvinje, Buzin, and Trešnjevka). The contents of TP, T, TF, and THD significantly varied depending on the collection site, and were in the range of 4.78%-12.85% (TP), 3.23%-5.80% (T), 0.42%-1.09% (TF), and 1.08%-2.59% (THD) of dry weight of plant material, with the Plitvice collection having the highest polyphenol content. GC-MS analysis of the essential oils showed a similarity in composition of the major compounds from all investigated populations. Fifty compounds were identified in all 4 investigat...

Periodicum Biologorum, Sep 1, 2010

Background and Purpose: Fraxinus americana L. and F. pennsylvanica were introduced 100 years ago ... more Background and Purpose: Fraxinus americana L. and F. pennsylvanica were introduced 100 years ago in forest management in the lowland regions of Croatia to afforestation sites where the native narrow-leaved ash could not survive. These species are not distinguished in the practice in Croatia. The aim of the article was to determine distinguishing traits between these introduced North American ash species based on morphological traits of samaras. Reliable determination is the necessary precondition for successful forest management of plantations of these two species. Materials and Methods: Samaras were collected from 50 trees in Croatian plantations on 5 localities (Karlovac, Dugo Selo, \ur|evac, Batina, Gunja), and from 3 voucher trees of F. americana and from 1 voucher tree of F. pennsylvanica. The following traits were measured: length of samara, length and width of samara wing, length and width of seed cavity, and the length of wing along the cavity. The results were evaluated using UPGMA method of cluster analysis with Euclidean distance (D E). Results: The most variable trait was absolute length of wing along seed cavity with coefficient variability (CV) from 8.38% to 23.41%, while the least variable trait was the ratio between the length of seed cavity and the length of samara (CV = 3.64-7.84%). Samaras from Croatian plantations showed similarity with F. pennsylvanica. Cluster analysis separated voucher trees of F. americana from trees from Croatian plantations of North American ash species, while the tree of F. pennsylvanica fitted among the trees from Croatian plantations. Conclusions: Only the presence of F. pennsylvanica in Croatian plantations was confirmed.

The relation between the contents of biologically active phenolic compounds (total polyphenols, T... more The relation between the contents of biologically active phenolic compounds (total polyphenols, TP; tannins, T; total flavonoids, TF; total phenolic acids, TPA) and soil traits in ten Moltkia petraea (Tratt.) Griseb. populations, growing in Croatia, Bosnia and Herzegovina, and Montenegro was investigated. The concentration of TP was 3.97–6.61 %, 3.91–6.07 %, and 3.26–5.53 % of dried weight in leaves, flowers, and stems, respectively. The content of TF was 0.37–1.13 %, 0.18–0.52 %, and 0.04–0.31 % in leaves, flowers and stems, while the content of TPA was 1.58–3.43 %, 1.71–3.26 %, and 1.50–2.92 % in the same organs (leaves, flowers, stems). The soil was characterized by very high content of organic matter (4.19–36.26 %) and by high variability in the content of CaCO3 (from below the limit of quantification to 50.59 %). pH (in 1 M solution of KCl) was 6.59–7.33, while the content of nitrogen was 0.33–0.70 %. The content of P2O5 and K2O was 1.22–11.09 mg per 100 g of soil and 26.61–125...

Zeitschrift für Naturforschung C, 2004

Micellar electrokinetic chromatography (MEKC) was used for the separation and determination of tw... more Micellar electrokinetic chromatography (MEKC) was used for the separation and determination of two iridoid glycosides, aucubin and catalpol, in several Plantago species growing in Croatia: P. altissima L., P. argentea Chaix, P. coronopus L., P. holosteum Scop. (subsp. depauperata, subsp. holosteum and subsp. scopulorum), P. lagopus L., P. lanceolata L., and P. maritima L. Hot water extraction (HWE) was applied for the isolation of iridoid substances. Significant differences appeared between the iridoid contents in the examined species. The yield of aucubin and catalpol was up to 0.27% and 1.81% of the dry mass of the leaves, respectively. Besides aucubin and catalpol, two related compounds were determined in the plant samples.

Zeitschrift für Naturforschung C, 2003

Hydride generation atomic absorption spectrometry (HGAAS) was applied for determination of seleni... more Hydride generation atomic absorption spectrometry (HGAAS) was applied for determination of selenium content in dried aerial parts of wild and cultivated Teucrium species (Lamiaceae) growing in Croatia: T. arduini L., T. chamaedrys L., T. flavum L., T. montanum L., T. polium L., and T. scordium L. subsp. scordioides Schreb. Special attention was paid to the wet oxidation procedure for the sample dissolution. The proposed procedure involved microwave-assisted sample digestion using a mixture of HNO3/H2O2. Wild specimens generally had a higher content of selenium, with concentrations of 0.030-0.095 mg/kg of the dry drug. Cultivated plants contained 0.020-0.055 mg Se/kg