Rezki amelia - Academia.edu (original) (raw)
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Experimental Cell Research, 1973
RNA synthesized in animal cells in the presence of high doses of actinomycin had many of the prop... more RNA synthesized in animal cells in the presence of high doses of actinomycin had many of the properties associated with hnRNA. The nucleoplasm was established as the site of this synthesis, as determined by radioautography and by differential extraction. No synthesis was observed in nucleoli. Therefore this RNA, which was unmethylated, did not represent abortively synthesized incomplete ribosomal RNA chains. Resistance to ethidium bromide also ruled out mitochondria as the site of synthesis of this RNA. RNA synthesized in the presence of actinomycin like hnRNA was resistant to inhibition by cordycepin. RNA synthesized in the presence of actinomycin contained a ribonuclease-resistant core. When first synthesized this RNA was associated with a high molecular weight RNA which was degraded rapidly. This unique species of RNA synthesized in the nucleoplasm of animal cells may exert a control function in the transcription of hnRNA in the cell nucleus or may play a role in the transport of mRNA from nucleus to cytoplasm.
Experimental Cell Research, 1973
RNA synthesized in animal cells in the presence of high doses of actinomycin had many of the prop... more RNA synthesized in animal cells in the presence of high doses of actinomycin had many of the properties associated with hnRNA. The nucleoplasm was established as the site of this synthesis, as determined by radioautography and by differential extraction. No synthesis was observed in nucleoli. Therefore this RNA, which was unmethylated, did not represent abortively synthesized incomplete ribosomal RNA chains. Resistance to ethidium bromide also ruled out mitochondria as the site of synthesis of this RNA. RNA synthesized in the presence of actinomycin like hnRNA was resistant to inhibition by cordycepin. RNA synthesized in the presence of actinomycin contained a ribonuclease-resistant core. When first synthesized this RNA was associated with a high molecular weight RNA which was degraded rapidly. This unique species of RNA synthesized in the nucleoplasm of animal cells may exert a control function in the transcription of hnRNA in the cell nucleus or may play a role in the transport of mRNA from nucleus to cytoplasm.