Ricardo Teles - Academia.edu (original) (raw)

Papers by Ricardo Teles

Http Dx Doi Org 10 1080 17480272 2012 700644, Jun 14, 2013

Nondestructive testing (NDT) can play an important role in improving the quality and reliability ... more Nondestructive testing (NDT) can play an important role in improving the quality and reliability of tropical hardwood as an engineering material. By means of these methods, the stiffness of the material can be determined and the information used to improve its structural performance. Although, it is a usual approach for qualifying the material used to manufacture engineered wood products made

Journal of clinical periodontology, Jan 3, 2016

The goal of this study was to identify progressing periodontal sites by applying linear mixed mod... more The goal of this study was to identify progressing periodontal sites by applying linear mixed models (LMM) to longitudinal measurements of clinical attachment loss (CAL). 93 periodontally healthy and 236 periodontitis subjects had their CAL measured bi-monthly for 12 months. The proportions of sites demonstrating increases in CAL from baseline above specified thresholds were calculated for each visit. The proportions of sites reversing from the progressing state were also computed. LMM were fitted for each tooth site and the predicted CAL levels used to categorize sites regarding progression or regression. The threshold for progression was established based on the model-estimated error in predictions. Over 12 months, 21.2%, 2.8%, and 0.3% of sites progressed, according to thresholds of 1, 2, and 3mm of CAL increase. However, on average, 42.0%, 64.4% and 77.7% of progressing sites for the different thresholds reversed in subsequent visits. Conversely, 97.1%, 76.9% and 23.1% of sites ...

Journal of Dental Research, Sep 1, 2009

Periodontal diseases, such as gingivitis and periodontitis, are characterized by bacterial plaque... more Periodontal diseases, such as gingivitis and periodontitis, are characterized by bacterial plaque accumulation around the gingival crevice and the subsequent inflammation and destruction of host tissues. To test the hypothesis that cellular metabolism is altered as a result of host-bacteria interaction, we performed an unbiased metabolomic profiling of gingival crevicular fluid (GCF) collected from healthy, gingivitis, and periodontitis sites in humans, by liquid and gas chromatography mass spectrometry. The purine degradation pathway, a major biochemical source for reactive oxygen species (ROS) production, was significantly accelerated at the disease sites. This suggests that periodontal-disease-induced oxidative stress and inflammation are mediated through this pathway. The complex host-bacterial interaction was further highlighted by depletion of anti-oxidants, degradation of host cellular components, and accumulation of bacterial products in GCF. These findings provide new mechanistic insights and a panel of comprehensive biomarkers for periodontal disease progression.

Journal of clinical periodontology, Jan 28, 2016

This exploratory randomized, controlled clinical trial sought to evaluate anti-inflammatory and -... more This exploratory randomized, controlled clinical trial sought to evaluate anti-inflammatory and -microbial effects of triclosan during experimental gingivitis as assessed by host response biomarkers and biofilm microbial pathogens. Thirty participants were randomized to triclosan or control dentifrice groups who ceased homecare for 21 days in an experimental gingivitis (EG) protocol. Plaque and gingival indices and saliva, plaque, and gingival crevicular fluid (GCF) were assessed/collected at days 0, 14, 21 and 35. Levels and proportions of 40 bacterial species from plaque samples were determined using checkerboard DNA-DNA hybridization. Ten biomarkers associated with inflammation, matrix degradation, and host protection were measured from GCF and saliva and analyzed using a multiplex array. Participants were stratified as 'high' or 'low' responders based on gingival index and GCF biomarkers and bacterial biofilm were combined to generate receiver operating character...

Journal of Periodontology, 2015

A subset of periodontal microorganisms has been associated with cardiovascular disease (CVD), whi... more A subset of periodontal microorganisms has been associated with cardiovascular disease (CVD), which is the leading complication of type 1 diabetes. We therefore evaluated the association between periodontal microorganism groups and early markers of CVD in youth with type 1 diabetes. A cross-sectional analysis was conducted among youth aged 12-19 years old at enrollment, 105 had type1 diabetes for at least 5 years and were seeking care at the Barbara Davis Center, University of Colorado from 2009 through 2011, and 71 did not have diabetes. Subgingival plaque samples were assessed for counts of 41 periodontal microorganisms using DNA-DNA hybridization. Microorganisms were classified into 4 groups using cluster analysis named Red-Orange, Orange-Green, Blue-Other, and Yellow-Other modified from Socransky's color scheme for periodontal microorganisms. Subsamples (n=54 with type1 diabetes, and n=48 without diabetes) also received a periodontal examination at the University of Colorado, School of Dental Medicine. Participants were approximately 15 years old on average and 74% were white. Mean periodontal pocket depth was 2 mm (se=0.02), and 17% had bleeding on probing. In multivariable analyses HbA1c was inversely associated with the Yellow-Other cluster (consisting of microorganisms that are not associated with periodontal disease) among youth with type1 diabetes. Blood pressure, triglycerides, LDL, HDL, and total cholesterol were not associated with microorganism clusters in this group. HbA1c was not associated with periodontal microorganism clusters among youth without diabetes. Among youth with type 1 diabetes who had good oral health, periodontal microorganisms were not associated with CVD risk factors.

Genome medicine, Jan 27, 2015

Infection and Immunity

Specific immunity has been implicated in the pathogenesis of periapical lesions, although the ext... more Specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. To investigate this question we compared periapical-lesion pathogenesis in RAG-2 severe combined immunodeficient (SCID) mice with immunocompetent control mice following surgical pulp exposure. In order to equalize the bacterial challenge, an infection protocol using Prevotella intermedia, Fusobacterium nucleatum, Peptostreptococcus micros, and Streptococcus intermedius was devised. The results demonstrated that after infection, the proportion of the root canal flora represented by the four pathogens was almost identical in both groups (39.9 and 42.2% for RAG-2 and immunocompetent control mice, respectively). The effects of abrogation of T- and B-cell mechanisms on periapical pathogenesis were then assessed. Approximately one-third of the RAG-2 mic...

Objectives: As the periodontal science has shown, gingival crevicular fluid (GCF) in sites with p... more Objectives: As the periodontal science has shown, gingival crevicular fluid (GCF) in sites with periodontal disease normally presents increased. Thus, the aim of this study was to investigate the amount of gingival crevicular fluid (GCF) in mesial/distal faces of superior cuspids. from Methods: The GCF was collected from 14 subjects (3 males and 11 females) submitted to orthodontic tooth movement (retraction) with a standard absorbent paper stripe (Periopaper) and measured by Periotron, in seven distinct periods (day -7, day 0, 1 hour, 24 hours, 14 days, 21 days and 80 days). The Student t Test was performed in order to compare the collected data (p<0.05). Results: The results shown that distal sites present higher amounts of GCF (pression area) than mesial sites (tension area). The highest period was 80 days after the beginning of the orthodontic tooth movement and the lowest, in 24 hours of activation. Conclusion: After all, we concluded that, as in periodontal disease sites, t...

Objectives: To determine if Fusobacterium nucleatum spp. nucleatum in vitro biofilm formation ind... more Objectives: To determine if Fusobacterium nucleatum spp. nucleatum in vitro biofilm formation induces β-lactamase production and to test if β-lactamase producing F. nucleatum biofilms could confer β-lactam resistance to β-lactam sensitive species such as Streptococcus pyogenes. Methods: F. nucleatum biofilms were grown on Calgary Biofilm Device for 6 days and exposed to different concentrations of amoxicillin to determine the biofilm inhibitory concentration (BIC). An inoculum of planktonically grown F. nucleatum with the same number of cells as the biofilm was used to test the minimum inhibitory concentration (MIC). Conversion of chromogenic nitrocefin was used to detect β-lactamase in the supernatant of the biofilms and planktonic cells. The number of cells in the biofilm and cells grown planktonically was determined using checkerboard DNA-DNA hybridization. The amoxicillin MIC of S. pyogenes was measured in presence or absence of a 7-day old F. nucleatum biofilm. Results: 1) F. n...

Objective: Prevalence data generated using the Human Oral Microbial Identification Microarray hav... more Objective: Prevalence data generated using the Human Oral Microbial Identification Microarray have implicated certain uncultivated/unrecognized bacterial taxa in the etiology of periodontitis. The goal of the present study was to examine levels of these uncultivated/unrecognized taxa in periodontal health and disease subjects. Method: 17 oligonucleotide probes targeting 30 uncultivated/unrecognized taxa were employed. Probes for 4 cultivated taxa and a Eubacterial probe were also used. Subgingival biofilm samples from 8 periodontitis (n=56) and 4 periodontally healthy (n=28) subjects were individually examined using the RNA-oligonucleotide quantification technique. Signals were converted to picomolars (pM) of RNA based on standard curves. Significance of differences between groups was tested using the Mann-Whitney test. Oral taxon (OT) designations were used in accord with the Human Oral Microbiome Database. Result: The levels of P.gingivalis, F.nucleatum and the uncultivated/unreco...

Fanconi anemia (FA) is a genetic disorder that leads to increased cancer predisposition due to hy... more Fanconi anemia (FA) is a genetic disorder that leads to increased cancer predisposition due to hypersensitivity to DNA damage. FA patients develop oral squamous cell carcinoma much earlier and more frequently than the general population. Previous studies have suggested an association between oral microorganisms and cancer. Objectives: to examine the salivary microbiota of FA patients and their non-FA relatives. Methods: Levels and proportions of 108 microbial taxa were determined in unstimulated whole saliva using checkerboard DNA- DNA hybridization. Prevalence of uncultivated/unrecognized was assessed using the human oral microbial identification microarray (HOMIM). Significant differences between groups were sought using the Mann-Whitney test. Results: FA patients (n=18) were younger (mean age± SD; 18.9±9.8 years) than non-FA subjects (n=8) (32.1±19.8), but gender distribution was similar (61% and 62%females, respectively). FA patients presented higher microbial load (mean total D...

Objective: to compare the microbiota of endodontic infections in necrotic pulp from HIV negative ... more Objective: to compare the microbiota of endodontic infections in necrotic pulp from HIV negative and HIV positive subjects. Method: Root canal samples from necrotic pulp were collected from 40 HIV- and 20 HIV+ subjects. Samples were amplified using multiple displacement amplification (MDA). Then, checkerboard DNA-DNA hybridization was employed to assess the levels of 107 microbial taxa. The percentage of DNA probe count and the percentage of teeth colonized by each test species were investigated. Significant differences between groups regarding proportions of taxa and prevalence of the test species was sought using the Mann Whitney test and the Chi-square analysis, respectively. Result: Among the most prevalent taxa detected were Dialister pneumosintes, Stenotrophomonas maltophilia, Corynebacterium diphtheriae, and Helicobacter pylori among HIV- subjects and D. pneumosintes, Prevotella tannerae, Prevotella gingivalis, and Corynebacterium diphtheriae among HIV+ individuals. D. pneumo...

Objectives: The aim of this study was to examine relationships among clinical parameters of perio... more Objectives: The aim of this study was to examine relationships among clinical parameters of periodontitis, measures of obesity, serum levels of adipokines and serum C-reactive protein (CRP) in chronic periodontitis subjects. Methods: 120 chronic periodontitis subjects were recruited and after a periodontal examination had their % body fat (BF) and body mass index (BMI) measured. Serum samples were collected and tested for levels of Adiponectin, Leptin, Resistin and CRP using ELISA. Correlations among clinical and serological parameters were examined using the Spearman correlation coefficient. Subjects were classified as having severe periodontitis if their mean pocket depth (PD) was >3.4mm (upper quartile of the distribution, n=29). Logistic regression was used to determine parameters associated with an increased risk for severe periodontitis. Results: Statistically significant positive correlations among mean serum levels of CRP and mean attachment level (AL) (rs=0.23), BF (rs=0...

Cancer causes & control : CCC, Jan 3, 2015

Periodontitis, a chronic inflammatory response to pathogenic bacteria in the oral microbiome, is ... more Periodontitis, a chronic inflammatory response to pathogenic bacteria in the oral microbiome, is common among adults. It is associated with several medical conditions, including cardiovascular diseases, and potentially with esophageal, lung, oral, and pancreatic cancer. One of the proposed mechanisms behind these associations is systemic inflammation, which has also been implicated in ovarian cancer etiology. The aim of this study was to evaluate association between ovarian cancer and periodontal bone loss. The association between periodontal bone loss, a marker of periodontitis, and risk of epithelial ovarian cancer was estimated among 60,560 participants of the prospective Nurses' Health Study using Cox proportional hazards analysis. Competing risks analysis was used to estimate association by histologic subtype. We did not observe an increased risk of ovarian cancer among participants with periodontal bone loss (HR 0.86, 95 % CI 0.64-1.15). Among women younger than 69 years, ...

The Journal of clinical dentistry, 2009

The purpose of the present investigation was to determine if antimicrobial mouthrinses with diffe... more The purpose of the present investigation was to determine if antimicrobial mouthrinses with different formulations could affect the composition of the subgingival microbiota and clinical parameters of adjacent tissues in periodontal maintenance subjects. One-hundred and sixteen subjects, who had been treated for chronic periodontitis and were in a maintenance program, were randomly assigned one of four mouthrinses, to be used twice daily for three months. The mouthrinses were herbal 1, herbal 2, essential oil, and chlorhexidine. Clinical measurements and subgingival plaque samples were taken at baseline and at three months. Plaque samples were individually evaluated for 18 test species/taxa using checkerboard DNA-DNA hybridization. Significance of differences between baseline and three months for both microbiological and clinical parameters were determined using the Wilcoxon Signed Ranks test. Significance of difference among groups for change in clinical and microbiological paramet...

Infection and immunity, 1997

Specific immunity has been implicated in the pathogenesis of periapical lesions, although the ext... more Specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. To investigate this question we compared periapical-lesion pathogenesis in RAG-2 severe combined immunodeficient (SCID) mice with immunocompetent control mice following surgical pulp exposure. In order to equalize the bacterial challenge, an infection protocol using Prevotella intermedia, Fusobacterium nucleatum, Peptostreptococcus micros, and Streptococcus intermedius was devised. The results demonstrated that after infection, the proportion of the root canal flora represented by the four pathogens was almost identical in both groups (39.9 and 42.2% for RAG-2 and immunocompetent control mice, respectively). The effects of abrogation of T- and B-cell mechanisms on periapical pathogenesis were then assessed. Approximately one-third of the RAG-2 mic...

Http Dx Doi Org 10 1080 17480272 2012 700644, Jun 14, 2013

Nondestructive testing (NDT) can play an important role in improving the quality and reliability ... more Nondestructive testing (NDT) can play an important role in improving the quality and reliability of tropical hardwood as an engineering material. By means of these methods, the stiffness of the material can be determined and the information used to improve its structural performance. Although, it is a usual approach for qualifying the material used to manufacture engineered wood products made

Journal of clinical periodontology, Jan 3, 2016

The goal of this study was to identify progressing periodontal sites by applying linear mixed mod... more The goal of this study was to identify progressing periodontal sites by applying linear mixed models (LMM) to longitudinal measurements of clinical attachment loss (CAL). 93 periodontally healthy and 236 periodontitis subjects had their CAL measured bi-monthly for 12 months. The proportions of sites demonstrating increases in CAL from baseline above specified thresholds were calculated for each visit. The proportions of sites reversing from the progressing state were also computed. LMM were fitted for each tooth site and the predicted CAL levels used to categorize sites regarding progression or regression. The threshold for progression was established based on the model-estimated error in predictions. Over 12 months, 21.2%, 2.8%, and 0.3% of sites progressed, according to thresholds of 1, 2, and 3mm of CAL increase. However, on average, 42.0%, 64.4% and 77.7% of progressing sites for the different thresholds reversed in subsequent visits. Conversely, 97.1%, 76.9% and 23.1% of sites ...

Journal of Dental Research, Sep 1, 2009

Periodontal diseases, such as gingivitis and periodontitis, are characterized by bacterial plaque... more Periodontal diseases, such as gingivitis and periodontitis, are characterized by bacterial plaque accumulation around the gingival crevice and the subsequent inflammation and destruction of host tissues. To test the hypothesis that cellular metabolism is altered as a result of host-bacteria interaction, we performed an unbiased metabolomic profiling of gingival crevicular fluid (GCF) collected from healthy, gingivitis, and periodontitis sites in humans, by liquid and gas chromatography mass spectrometry. The purine degradation pathway, a major biochemical source for reactive oxygen species (ROS) production, was significantly accelerated at the disease sites. This suggests that periodontal-disease-induced oxidative stress and inflammation are mediated through this pathway. The complex host-bacterial interaction was further highlighted by depletion of anti-oxidants, degradation of host cellular components, and accumulation of bacterial products in GCF. These findings provide new mechanistic insights and a panel of comprehensive biomarkers for periodontal disease progression.

Journal of clinical periodontology, Jan 28, 2016

This exploratory randomized, controlled clinical trial sought to evaluate anti-inflammatory and -... more This exploratory randomized, controlled clinical trial sought to evaluate anti-inflammatory and -microbial effects of triclosan during experimental gingivitis as assessed by host response biomarkers and biofilm microbial pathogens. Thirty participants were randomized to triclosan or control dentifrice groups who ceased homecare for 21 days in an experimental gingivitis (EG) protocol. Plaque and gingival indices and saliva, plaque, and gingival crevicular fluid (GCF) were assessed/collected at days 0, 14, 21 and 35. Levels and proportions of 40 bacterial species from plaque samples were determined using checkerboard DNA-DNA hybridization. Ten biomarkers associated with inflammation, matrix degradation, and host protection were measured from GCF and saliva and analyzed using a multiplex array. Participants were stratified as 'high' or 'low' responders based on gingival index and GCF biomarkers and bacterial biofilm were combined to generate receiver operating character...

Journal of Periodontology, 2015

A subset of periodontal microorganisms has been associated with cardiovascular disease (CVD), whi... more A subset of periodontal microorganisms has been associated with cardiovascular disease (CVD), which is the leading complication of type 1 diabetes. We therefore evaluated the association between periodontal microorganism groups and early markers of CVD in youth with type 1 diabetes. A cross-sectional analysis was conducted among youth aged 12-19 years old at enrollment, 105 had type1 diabetes for at least 5 years and were seeking care at the Barbara Davis Center, University of Colorado from 2009 through 2011, and 71 did not have diabetes. Subgingival plaque samples were assessed for counts of 41 periodontal microorganisms using DNA-DNA hybridization. Microorganisms were classified into 4 groups using cluster analysis named Red-Orange, Orange-Green, Blue-Other, and Yellow-Other modified from Socransky&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s color scheme for periodontal microorganisms. Subsamples (n=54 with type1 diabetes, and n=48 without diabetes) also received a periodontal examination at the University of Colorado, School of Dental Medicine. Participants were approximately 15 years old on average and 74% were white. Mean periodontal pocket depth was 2 mm (se=0.02), and 17% had bleeding on probing. In multivariable analyses HbA1c was inversely associated with the Yellow-Other cluster (consisting of microorganisms that are not associated with periodontal disease) among youth with type1 diabetes. Blood pressure, triglycerides, LDL, HDL, and total cholesterol were not associated with microorganism clusters in this group. HbA1c was not associated with periodontal microorganism clusters among youth without diabetes. Among youth with type 1 diabetes who had good oral health, periodontal microorganisms were not associated with CVD risk factors.

Genome medicine, Jan 27, 2015

Infection and Immunity

Specific immunity has been implicated in the pathogenesis of periapical lesions, although the ext... more Specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. To investigate this question we compared periapical-lesion pathogenesis in RAG-2 severe combined immunodeficient (SCID) mice with immunocompetent control mice following surgical pulp exposure. In order to equalize the bacterial challenge, an infection protocol using Prevotella intermedia, Fusobacterium nucleatum, Peptostreptococcus micros, and Streptococcus intermedius was devised. The results demonstrated that after infection, the proportion of the root canal flora represented by the four pathogens was almost identical in both groups (39.9 and 42.2% for RAG-2 and immunocompetent control mice, respectively). The effects of abrogation of T- and B-cell mechanisms on periapical pathogenesis were then assessed. Approximately one-third of the RAG-2 mic...

Objectives: As the periodontal science has shown, gingival crevicular fluid (GCF) in sites with p... more Objectives: As the periodontal science has shown, gingival crevicular fluid (GCF) in sites with periodontal disease normally presents increased. Thus, the aim of this study was to investigate the amount of gingival crevicular fluid (GCF) in mesial/distal faces of superior cuspids. from Methods: The GCF was collected from 14 subjects (3 males and 11 females) submitted to orthodontic tooth movement (retraction) with a standard absorbent paper stripe (Periopaper) and measured by Periotron, in seven distinct periods (day -7, day 0, 1 hour, 24 hours, 14 days, 21 days and 80 days). The Student t Test was performed in order to compare the collected data (p<0.05). Results: The results shown that distal sites present higher amounts of GCF (pression area) than mesial sites (tension area). The highest period was 80 days after the beginning of the orthodontic tooth movement and the lowest, in 24 hours of activation. Conclusion: After all, we concluded that, as in periodontal disease sites, t...

Objectives: To determine if Fusobacterium nucleatum spp. nucleatum in vitro biofilm formation ind... more Objectives: To determine if Fusobacterium nucleatum spp. nucleatum in vitro biofilm formation induces β-lactamase production and to test if β-lactamase producing F. nucleatum biofilms could confer β-lactam resistance to β-lactam sensitive species such as Streptococcus pyogenes. Methods: F. nucleatum biofilms were grown on Calgary Biofilm Device for 6 days and exposed to different concentrations of amoxicillin to determine the biofilm inhibitory concentration (BIC). An inoculum of planktonically grown F. nucleatum with the same number of cells as the biofilm was used to test the minimum inhibitory concentration (MIC). Conversion of chromogenic nitrocefin was used to detect β-lactamase in the supernatant of the biofilms and planktonic cells. The number of cells in the biofilm and cells grown planktonically was determined using checkerboard DNA-DNA hybridization. The amoxicillin MIC of S. pyogenes was measured in presence or absence of a 7-day old F. nucleatum biofilm. Results: 1) F. n...

Objective: Prevalence data generated using the Human Oral Microbial Identification Microarray hav... more Objective: Prevalence data generated using the Human Oral Microbial Identification Microarray have implicated certain uncultivated/unrecognized bacterial taxa in the etiology of periodontitis. The goal of the present study was to examine levels of these uncultivated/unrecognized taxa in periodontal health and disease subjects. Method: 17 oligonucleotide probes targeting 30 uncultivated/unrecognized taxa were employed. Probes for 4 cultivated taxa and a Eubacterial probe were also used. Subgingival biofilm samples from 8 periodontitis (n=56) and 4 periodontally healthy (n=28) subjects were individually examined using the RNA-oligonucleotide quantification technique. Signals were converted to picomolars (pM) of RNA based on standard curves. Significance of differences between groups was tested using the Mann-Whitney test. Oral taxon (OT) designations were used in accord with the Human Oral Microbiome Database. Result: The levels of P.gingivalis, F.nucleatum and the uncultivated/unreco...

Fanconi anemia (FA) is a genetic disorder that leads to increased cancer predisposition due to hy... more Fanconi anemia (FA) is a genetic disorder that leads to increased cancer predisposition due to hypersensitivity to DNA damage. FA patients develop oral squamous cell carcinoma much earlier and more frequently than the general population. Previous studies have suggested an association between oral microorganisms and cancer. Objectives: to examine the salivary microbiota of FA patients and their non-FA relatives. Methods: Levels and proportions of 108 microbial taxa were determined in unstimulated whole saliva using checkerboard DNA- DNA hybridization. Prevalence of uncultivated/unrecognized was assessed using the human oral microbial identification microarray (HOMIM). Significant differences between groups were sought using the Mann-Whitney test. Results: FA patients (n=18) were younger (mean age± SD; 18.9±9.8 years) than non-FA subjects (n=8) (32.1±19.8), but gender distribution was similar (61% and 62%females, respectively). FA patients presented higher microbial load (mean total D...

Objective: to compare the microbiota of endodontic infections in necrotic pulp from HIV negative ... more Objective: to compare the microbiota of endodontic infections in necrotic pulp from HIV negative and HIV positive subjects. Method: Root canal samples from necrotic pulp were collected from 40 HIV- and 20 HIV+ subjects. Samples were amplified using multiple displacement amplification (MDA). Then, checkerboard DNA-DNA hybridization was employed to assess the levels of 107 microbial taxa. The percentage of DNA probe count and the percentage of teeth colonized by each test species were investigated. Significant differences between groups regarding proportions of taxa and prevalence of the test species was sought using the Mann Whitney test and the Chi-square analysis, respectively. Result: Among the most prevalent taxa detected were Dialister pneumosintes, Stenotrophomonas maltophilia, Corynebacterium diphtheriae, and Helicobacter pylori among HIV- subjects and D. pneumosintes, Prevotella tannerae, Prevotella gingivalis, and Corynebacterium diphtheriae among HIV+ individuals. D. pneumo...

Objectives: The aim of this study was to examine relationships among clinical parameters of perio... more Objectives: The aim of this study was to examine relationships among clinical parameters of periodontitis, measures of obesity, serum levels of adipokines and serum C-reactive protein (CRP) in chronic periodontitis subjects. Methods: 120 chronic periodontitis subjects were recruited and after a periodontal examination had their % body fat (BF) and body mass index (BMI) measured. Serum samples were collected and tested for levels of Adiponectin, Leptin, Resistin and CRP using ELISA. Correlations among clinical and serological parameters were examined using the Spearman correlation coefficient. Subjects were classified as having severe periodontitis if their mean pocket depth (PD) was >3.4mm (upper quartile of the distribution, n=29). Logistic regression was used to determine parameters associated with an increased risk for severe periodontitis. Results: Statistically significant positive correlations among mean serum levels of CRP and mean attachment level (AL) (rs=0.23), BF (rs=0...

Cancer causes & control : CCC, Jan 3, 2015

Periodontitis, a chronic inflammatory response to pathogenic bacteria in the oral microbiome, is ... more Periodontitis, a chronic inflammatory response to pathogenic bacteria in the oral microbiome, is common among adults. It is associated with several medical conditions, including cardiovascular diseases, and potentially with esophageal, lung, oral, and pancreatic cancer. One of the proposed mechanisms behind these associations is systemic inflammation, which has also been implicated in ovarian cancer etiology. The aim of this study was to evaluate association between ovarian cancer and periodontal bone loss. The association between periodontal bone loss, a marker of periodontitis, and risk of epithelial ovarian cancer was estimated among 60,560 participants of the prospective Nurses' Health Study using Cox proportional hazards analysis. Competing risks analysis was used to estimate association by histologic subtype. We did not observe an increased risk of ovarian cancer among participants with periodontal bone loss (HR 0.86, 95 % CI 0.64-1.15). Among women younger than 69 years, ...

The Journal of clinical dentistry, 2009

The purpose of the present investigation was to determine if antimicrobial mouthrinses with diffe... more The purpose of the present investigation was to determine if antimicrobial mouthrinses with different formulations could affect the composition of the subgingival microbiota and clinical parameters of adjacent tissues in periodontal maintenance subjects. One-hundred and sixteen subjects, who had been treated for chronic periodontitis and were in a maintenance program, were randomly assigned one of four mouthrinses, to be used twice daily for three months. The mouthrinses were herbal 1, herbal 2, essential oil, and chlorhexidine. Clinical measurements and subgingival plaque samples were taken at baseline and at three months. Plaque samples were individually evaluated for 18 test species/taxa using checkerboard DNA-DNA hybridization. Significance of differences between baseline and three months for both microbiological and clinical parameters were determined using the Wilcoxon Signed Ranks test. Significance of difference among groups for change in clinical and microbiological paramet...

Infection and immunity, 1997

Specific immunity has been implicated in the pathogenesis of periapical lesions, although the ext... more Specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. To investigate this question we compared periapical-lesion pathogenesis in RAG-2 severe combined immunodeficient (SCID) mice with immunocompetent control mice following surgical pulp exposure. In order to equalize the bacterial challenge, an infection protocol using Prevotella intermedia, Fusobacterium nucleatum, Peptostreptococcus micros, and Streptococcus intermedius was devised. The results demonstrated that after infection, the proportion of the root canal flora represented by the four pathogens was almost identical in both groups (39.9 and 42.2% for RAG-2 and immunocompetent control mice, respectively). The effects of abrogation of T- and B-cell mechanisms on periapical pathogenesis were then assessed. Approximately one-third of the RAG-2 mic...