Rinaldo Gandra - Academia.edu (original) (raw)

Papers by Rinaldo Gandra

RESUMO O objetivo deste artigo foi apresentar uma revisão de literatura acerca da ação antimicrob... more RESUMO O objetivo deste artigo foi apresentar uma revisão de literatura acerca da ação antimicrobiana das micocinas produzidas por Wickerhamomyces anomalus. Uma busca em publicações de 2016 a 2021 foi realizada nas bases de dados Biblioteca Virtual em Saúde (BVS), Scientific Eletronic Library Online (SciElo) e Pubmed, cruzando-se os termos "Wickerhamomyces anomalus", "killer toxins" e "mycocins". Foi utilizado como critério de exclusão os artigos de revisão de literatura e aqueles não relacionados ao tema. Foram encontrados 16 artigos, e após uma leitura crítica de títulos e resumos, selecionouse 13 para a escrita deste artigo.

Background: The immunofluorescence pattern of antinuclear antibodies (ANA) called rings and rods ... more Background: The immunofluorescence pattern of antinuclear antibodies (ANA) called rings and rods (RR) has only recently been described. This pattern has been associated with patients with Hepatitis C at treatment. There are few studies that demonstrate their appearance in healthy individuals or with other health conditions. This study reports positivity of this autoantibody in a patient never exposed to the HCV virus, in order to understand its clinical significance. Case summary: A 10-year-old male patient was admitted to a university hospital with suspected bronchopneumonia. A carrier of chronic lung disease, he underwent several tests including ANA, that was reagent for the nucleus (fine speckled pattern) and cytoplasm (RR pattern). The serological results for hepatitis and HIV were negative. The patient was discharged after 22 days in hospital and is being followed-up by the outpatient clinic. There was a subsequent suspicion of immunodeficiency and cystic fibrosis, but there re...

Paracoccidioidomicose (PCM) e uma micose profunda, causada pelo fungo dimorfico Paracoccidioides ... more Paracoccidioidomicose (PCM) e uma micose profunda, causada pelo fungo dimorfico Paracoccidioides brasiliensis (Pb), a doenca acomete mais frequentemente individuos do sexo masculino, entre com idade superior a 30 anos e em fase produtiva da vida. A regiao do lago de Itaipu que abrange as fronteiras do Brasil, Paraguai e Argentina, parece possuir condicoes favoraveis para o desenvolvimento desde patogeno e assim tem apresentado autos indices desta doenca. O presente estudo objetivou investigar a incidencia da doenca e dos eventos de morte entre os portadores de PCM residentes na regiao do Lago de Itaipu no periodo de 2008 a 2009. Para tanto, foi realizado levantamento em registros e prontuarios de pacientes do Setor de Tuberculose do Centro de Epidemiologia da Cidade de Foz do Iguacu/Parana entre o periodo de janeiro de 2008 a julho de 2009. Os resultados apontam 102 novos casos de PCM no periodo descrito, destes 72 eram homens e 30 eram mulheres, foram registrados 15 obitos durante ...

The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containi... more The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containing the conserved β-Xylosidase and α-l-Arabinofuranosidase (β-Xyl I-α-l-Ara) domains was amplified by PCR and cloned into the vector pJet1.2Blunt. The xynB1 gene was subcloned into the vector pPROEX-hta that produces a histidine-fused translation product. The overexpression of recombinant β-Xyl I-α-l-Ara was induced with IPTG in BL21 (DE3) and the resulting intracellular protein was purified with pre-packaged nickel-Sepharose columns. The recombinant β-Xyl I-α-l-Ara exhibited a specific β-Xylosidase I activity of 1.25 U mg−1 to oNPX and a specific α-l-Arabinofuranosidase activity of 0.47 U mg−1 to pNPA. The predominant activity of the recombinant enzyme was its β-Xylosidase I activity, and the enzymatic characterization was focused on it. The β-Xylosidase I activity was high over the pH range 3–10, with maximal activity at pH 6. The enzyme activity was optimal at 45 °C, and a high degree ...

The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated fro... more The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possi...

Objectives The aim of presented cross-sectional and observational study was to determine the prev... more Objectives The aim of presented cross-sectional and observational study was to determine the prevalence of late oral complications of patients with head and neck cancer who underwent radiotherapy, by clinical and laboratory analyses. Material and Methods Fifty-five patients, 43 (78.2%) men and 12 (21.8%) women, mean age 60; range 38 to 87 years, who have completed radiotherapy for head and neck cancer for at least 6 months were enrolled. The presence of xerostomia, hyposalivation, oral candidiasis, and type of oral yeasts were correlated with post-radiotherapy period. A control group, age and gender matched, was used for comparisons. The Pearson’s Chi-square or Fischer’s exact test was used at a significance level of 5%. Results The mean post-radiotherapy period was 32 months. The oral complications found were xerostomia (45/55, [81.8%]), hyposalivation (44/55 [80%]) and oral candidiasis (15/55 [27.2%]). Xerostomia and hyposalivation was statistically higher in the study group when ...

Parasitology Research

The current treatment of leishmaniasis presents some problems, such as cell toxicity, parenteral ... more The current treatment of leishmaniasis presents some problems, such as cell toxicity, parenteral route, and time of treatment. Ozone emerges as an option to accelerate the standard treatment due to the immunomodulatory, antioxidant, and wound healing activity reported in the literature. This work aimed to evaluate the efficacy of aqueous ozone as an adjuvant to the standard treatment of cutaneous lesions caused by Leishmania amazonensis in an experimental model. For in vivo experiments, mice were randomly distributed in 6 groups, which were infected with L. amazonensis and treated in five different schedules using the standard treatment with Glucantime® with or without aqueous ozone. After the last day of treatment, the animals were euthanized and were analyzed: the thickness of lesions; collagen deposition, the parasitic burden of the lesions; blood leukocyte number; NO; and cytokine dosages and arginase activity from peritoneal macrophages. All treated groups showed a decrease in the lesion, but with a significative deposition of collagen in lesions with local ozone treatment. The parasite burden showed that ozone enhanced the leishmanicidal activity of the reference drug. The reduction of NO production and blood leukocyte count and increases in the arginase activity showed an immunomodulatory activity of ozone in the treated animals. Thus, ozone therapy has been shown to work as an adjuvant in the treatment of Leishmania lesions, enhancing leishmanicidal and wound healing activity of standard treatment.

Food Science and Technology

Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contamina... more Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contaminated with S. aureus coagulase positive is considered a public health risk because the bacterium is resistant to several conventional antimicrobials. Mycocins are substances produced by yeasts that secrete glycoproteins, which can also be called toxin killers, they have an inhibitory action on other microorganisms. The Wickerhamomyces anomalus, is one of the microorganisms capable of producing these mycocins, resulting in the action of disturbances on the cell wall of the pathogen causing deleterious effects. This work aims to evaluate the antimicrobial activity of the mycocins produced by W. anomalus WA45 against the 29 strains of S. aureus coagulase positive isolated from bovine, porcine and chicken meat and 1 standard strain. The antimicrobial action of the mycocins present on the culture supernatant of W. anomalus WA45 was tested by microdilution and the results were satisfactory, since 100% inhibition of strains of S. aureus coagulase positive. We concluded that the mycocins present in the supernatant of W. anomalus WA45 showed antimicrobial action, being candidates for the development of new products for the biocontrol and bioconservation of meat.

Journal of Oral and Maxillofacial Research

The aim of presented cross-sectional and observational study was to determine the prevalence of l... more The aim of presented cross-sectional and observational study was to determine the prevalence of late oral complications of patients with head and neck cancer who underwent radiotherapy, by clinical and laboratory analyses. Material and Methods: Fifty-five patients, 43 (78.2%) men and 12 (21.8%) women, mean age 60; range 38 to 87 years, who have completed radiotherapy for head and neck cancer for at least 6 months were enrolled. The presence of xerostomia, hyposalivation, oral candidiasis, and type of oral yeasts were correlated with post-radiotherapy period. A control group, age and gender matched, was used for comparisons. The Pearson's Chi-square or Fischer's exact test was used at a significance level of 5%. Results: The mean post-radiotherapy period was 32 months. The oral complications found were xerostomia (45/55, [81.8%]), hyposalivation (44/55 [80%]) and oral candidiasis (15/55 [27.2%]). Xerostomia and hyposalivation was statistically higher in the study group when compared to the control group (P < 0.05). The presence of yeast occurred in 39 (70.9%) of the patients in the study group, and Candida albicans was the most prevalent etiological agent in 25 (64.1%) of those patients (P < 0.05). Conclusions: Xerostomia and hyposalivation were the more prevalent late oral complications related to radiotherapy. Oral candidiasis was also observed, although its prevalence was lower. The need for long-term dental follow-up of patients who underwent radiotherapy of the head and neck cancer is mandatory.

Molecular Biology Reports

Arquivos de Ciências da Saúde da UNIPAR

A presença de enteroparasitoses em crianças em idade pré-escolar tem sido motivo de preocupação p... more A presença de enteroparasitoses em crianças em idade pré-escolar tem sido motivo de preocupação pelos órgãos de saúde principalmente devido ao impacto negativo sobre o crescimento e desenvolvimento intelectual dessas crianças. As infecções por parasitas intestinais podem levar a quadros de desnutrição e formas de gastroenterite crônica. O presente trabalho analisou crianças em idade pré-escolar frequentadoras de 11 unidades CMEIs (Centros Municipais de Educação Infantil) na cidade de Guarapuava-PR em 11 unidades. Foram estudadas 287 crianças e o índice de positividade para enteroparasitoses foi de 13,9%. A idade média das 287 crianças foi de 2,7 anos e a maioria das crianças analisadas foram domicialiadas em zona urbana 84,3% e somente 15,7% em zona rural, com média de filhos por família de 2,08 membros. A prevalência das parasitoses mostrou que G. duodenalis foi o protozoário mais isolado com 70,4%, seguido de Ascaris lumbricoides 6,8% o único helminto encontrado. Com relação à ter...

Scientia Medica

AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatmen... more AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when com...

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin Americ... more Introduction: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. This study aimed to evaluate the natural history of Paracoccidioides brasiliensis-induced experimental arthritis of the knee joints in Wistar rats. Methods: Rats were randomly allocated to either an absolute control group, or 15-day, 45-day, or 90-day experimental (fungusinoculated) groups. Results: Experimental groups developed classic signs of articular PCM. Titers of anti-gp43 were observed to increase during the interval from 15 to 45 days post-inoculation. Conclusions: Articular arthritic lesions were induced and progressed during the study period in all experimental groups.

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: Acrylic resins are used in the preparation of facial prostheses and may be colonize... more Introduction: Acrylic resins are used in the preparation of facial prostheses and may be colonized by fungi. Here, we verified the antifungal efficacy of this material after surface treatment using poly (diallyldimethylammonium chloride). Methods: Acrylic resin specimens with and without surface treatment were subjected to tests for fungistatic and fungicidal activities. Standard strains of Candida albicans and Aspergillus niger were used. Results: After surface treatment, the fungistatic and fungicidal efficacies of the resins against C. albicans and fungistatic action against A. niger were verified. Conclusions: The surface treatment was a determinant of the antifungal activity of the material.

Journal of Advances in Biology & Biotechnology

The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated fro... more The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possi...

RESUMO O objetivo deste artigo foi apresentar uma revisão de literatura acerca da ação antimicrob... more RESUMO O objetivo deste artigo foi apresentar uma revisão de literatura acerca da ação antimicrobiana das micocinas produzidas por Wickerhamomyces anomalus. Uma busca em publicações de 2016 a 2021 foi realizada nas bases de dados Biblioteca Virtual em Saúde (BVS), Scientific Eletronic Library Online (SciElo) e Pubmed, cruzando-se os termos "Wickerhamomyces anomalus", "killer toxins" e "mycocins". Foi utilizado como critério de exclusão os artigos de revisão de literatura e aqueles não relacionados ao tema. Foram encontrados 16 artigos, e após uma leitura crítica de títulos e resumos, selecionouse 13 para a escrita deste artigo.

Background: The immunofluorescence pattern of antinuclear antibodies (ANA) called rings and rods ... more Background: The immunofluorescence pattern of antinuclear antibodies (ANA) called rings and rods (RR) has only recently been described. This pattern has been associated with patients with Hepatitis C at treatment. There are few studies that demonstrate their appearance in healthy individuals or with other health conditions. This study reports positivity of this autoantibody in a patient never exposed to the HCV virus, in order to understand its clinical significance. Case summary: A 10-year-old male patient was admitted to a university hospital with suspected bronchopneumonia. A carrier of chronic lung disease, he underwent several tests including ANA, that was reagent for the nucleus (fine speckled pattern) and cytoplasm (RR pattern). The serological results for hepatitis and HIV were negative. The patient was discharged after 22 days in hospital and is being followed-up by the outpatient clinic. There was a subsequent suspicion of immunodeficiency and cystic fibrosis, but there re...

Paracoccidioidomicose (PCM) e uma micose profunda, causada pelo fungo dimorfico Paracoccidioides ... more Paracoccidioidomicose (PCM) e uma micose profunda, causada pelo fungo dimorfico Paracoccidioides brasiliensis (Pb), a doenca acomete mais frequentemente individuos do sexo masculino, entre com idade superior a 30 anos e em fase produtiva da vida. A regiao do lago de Itaipu que abrange as fronteiras do Brasil, Paraguai e Argentina, parece possuir condicoes favoraveis para o desenvolvimento desde patogeno e assim tem apresentado autos indices desta doenca. O presente estudo objetivou investigar a incidencia da doenca e dos eventos de morte entre os portadores de PCM residentes na regiao do Lago de Itaipu no periodo de 2008 a 2009. Para tanto, foi realizado levantamento em registros e prontuarios de pacientes do Setor de Tuberculose do Centro de Epidemiologia da Cidade de Foz do Iguacu/Parana entre o periodo de janeiro de 2008 a julho de 2009. Os resultados apontam 102 novos casos de PCM no periodo descrito, destes 72 eram homens e 30 eram mulheres, foram registrados 15 obitos durante ...

The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containi... more The xynB1 gene (CCNA 01040) of Caulobacter crescentus that encodes a bifunctional enzyme containing the conserved β-Xylosidase and α-l-Arabinofuranosidase (β-Xyl I-α-l-Ara) domains was amplified by PCR and cloned into the vector pJet1.2Blunt. The xynB1 gene was subcloned into the vector pPROEX-hta that produces a histidine-fused translation product. The overexpression of recombinant β-Xyl I-α-l-Ara was induced with IPTG in BL21 (DE3) and the resulting intracellular protein was purified with pre-packaged nickel-Sepharose columns. The recombinant β-Xyl I-α-l-Ara exhibited a specific β-Xylosidase I activity of 1.25 U mg−1 to oNPX and a specific α-l-Arabinofuranosidase activity of 0.47 U mg−1 to pNPA. The predominant activity of the recombinant enzyme was its β-Xylosidase I activity, and the enzymatic characterization was focused on it. The β-Xylosidase I activity was high over the pH range 3–10, with maximal activity at pH 6. The enzyme activity was optimal at 45 °C, and a high degree ...

The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated fro... more The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possi...

Objectives The aim of presented cross-sectional and observational study was to determine the prev... more Objectives The aim of presented cross-sectional and observational study was to determine the prevalence of late oral complications of patients with head and neck cancer who underwent radiotherapy, by clinical and laboratory analyses. Material and Methods Fifty-five patients, 43 (78.2%) men and 12 (21.8%) women, mean age 60; range 38 to 87 years, who have completed radiotherapy for head and neck cancer for at least 6 months were enrolled. The presence of xerostomia, hyposalivation, oral candidiasis, and type of oral yeasts were correlated with post-radiotherapy period. A control group, age and gender matched, was used for comparisons. The Pearson’s Chi-square or Fischer’s exact test was used at a significance level of 5%. Results The mean post-radiotherapy period was 32 months. The oral complications found were xerostomia (45/55, [81.8%]), hyposalivation (44/55 [80%]) and oral candidiasis (15/55 [27.2%]). Xerostomia and hyposalivation was statistically higher in the study group when ...

Parasitology Research

The current treatment of leishmaniasis presents some problems, such as cell toxicity, parenteral ... more The current treatment of leishmaniasis presents some problems, such as cell toxicity, parenteral route, and time of treatment. Ozone emerges as an option to accelerate the standard treatment due to the immunomodulatory, antioxidant, and wound healing activity reported in the literature. This work aimed to evaluate the efficacy of aqueous ozone as an adjuvant to the standard treatment of cutaneous lesions caused by Leishmania amazonensis in an experimental model. For in vivo experiments, mice were randomly distributed in 6 groups, which were infected with L. amazonensis and treated in five different schedules using the standard treatment with Glucantime® with or without aqueous ozone. After the last day of treatment, the animals were euthanized and were analyzed: the thickness of lesions; collagen deposition, the parasitic burden of the lesions; blood leukocyte number; NO; and cytokine dosages and arginase activity from peritoneal macrophages. All treated groups showed a decrease in the lesion, but with a significative deposition of collagen in lesions with local ozone treatment. The parasite burden showed that ozone enhanced the leishmanicidal activity of the reference drug. The reduction of NO production and blood leukocyte count and increases in the arginase activity showed an immunomodulatory activity of ozone in the treated animals. Thus, ozone therapy has been shown to work as an adjuvant in the treatment of Leishmania lesions, enhancing leishmanicidal and wound healing activity of standard treatment.

Food Science and Technology

Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contamina... more Staphylococcus aureus is among the major pathogens involved in food poisoning, and meat contaminated with S. aureus coagulase positive is considered a public health risk because the bacterium is resistant to several conventional antimicrobials. Mycocins are substances produced by yeasts that secrete glycoproteins, which can also be called toxin killers, they have an inhibitory action on other microorganisms. The Wickerhamomyces anomalus, is one of the microorganisms capable of producing these mycocins, resulting in the action of disturbances on the cell wall of the pathogen causing deleterious effects. This work aims to evaluate the antimicrobial activity of the mycocins produced by W. anomalus WA45 against the 29 strains of S. aureus coagulase positive isolated from bovine, porcine and chicken meat and 1 standard strain. The antimicrobial action of the mycocins present on the culture supernatant of W. anomalus WA45 was tested by microdilution and the results were satisfactory, since 100% inhibition of strains of S. aureus coagulase positive. We concluded that the mycocins present in the supernatant of W. anomalus WA45 showed antimicrobial action, being candidates for the development of new products for the biocontrol and bioconservation of meat.

Journal of Oral and Maxillofacial Research

The aim of presented cross-sectional and observational study was to determine the prevalence of l... more The aim of presented cross-sectional and observational study was to determine the prevalence of late oral complications of patients with head and neck cancer who underwent radiotherapy, by clinical and laboratory analyses. Material and Methods: Fifty-five patients, 43 (78.2%) men and 12 (21.8%) women, mean age 60; range 38 to 87 years, who have completed radiotherapy for head and neck cancer for at least 6 months were enrolled. The presence of xerostomia, hyposalivation, oral candidiasis, and type of oral yeasts were correlated with post-radiotherapy period. A control group, age and gender matched, was used for comparisons. The Pearson's Chi-square or Fischer's exact test was used at a significance level of 5%. Results: The mean post-radiotherapy period was 32 months. The oral complications found were xerostomia (45/55, [81.8%]), hyposalivation (44/55 [80%]) and oral candidiasis (15/55 [27.2%]). Xerostomia and hyposalivation was statistically higher in the study group when compared to the control group (P < 0.05). The presence of yeast occurred in 39 (70.9%) of the patients in the study group, and Candida albicans was the most prevalent etiological agent in 25 (64.1%) of those patients (P < 0.05). Conclusions: Xerostomia and hyposalivation were the more prevalent late oral complications related to radiotherapy. Oral candidiasis was also observed, although its prevalence was lower. The need for long-term dental follow-up of patients who underwent radiotherapy of the head and neck cancer is mandatory.

Molecular Biology Reports

Arquivos de Ciências da Saúde da UNIPAR

A presença de enteroparasitoses em crianças em idade pré-escolar tem sido motivo de preocupação p... more A presença de enteroparasitoses em crianças em idade pré-escolar tem sido motivo de preocupação pelos órgãos de saúde principalmente devido ao impacto negativo sobre o crescimento e desenvolvimento intelectual dessas crianças. As infecções por parasitas intestinais podem levar a quadros de desnutrição e formas de gastroenterite crônica. O presente trabalho analisou crianças em idade pré-escolar frequentadoras de 11 unidades CMEIs (Centros Municipais de Educação Infantil) na cidade de Guarapuava-PR em 11 unidades. Foram estudadas 287 crianças e o índice de positividade para enteroparasitoses foi de 13,9%. A idade média das 287 crianças foi de 2,7 anos e a maioria das crianças analisadas foram domicialiadas em zona urbana 84,3% e somente 15,7% em zona rural, com média de filhos por família de 2,08 membros. A prevalência das parasitoses mostrou que G. duodenalis foi o protozoário mais isolado com 70,4%, seguido de Ascaris lumbricoides 6,8% o único helminto encontrado. Com relação à ter...

Scientia Medica

AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatmen... more AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when com...

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin Americ... more Introduction: Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. This study aimed to evaluate the natural history of Paracoccidioides brasiliensis-induced experimental arthritis of the knee joints in Wistar rats. Methods: Rats were randomly allocated to either an absolute control group, or 15-day, 45-day, or 90-day experimental (fungusinoculated) groups. Results: Experimental groups developed classic signs of articular PCM. Titers of anti-gp43 were observed to increase during the interval from 15 to 45 days post-inoculation. Conclusions: Articular arthritic lesions were induced and progressed during the study period in all experimental groups.

Revista da Sociedade Brasileira de Medicina Tropical

Introduction: Acrylic resins are used in the preparation of facial prostheses and may be colonize... more Introduction: Acrylic resins are used in the preparation of facial prostheses and may be colonized by fungi. Here, we verified the antifungal efficacy of this material after surface treatment using poly (diallyldimethylammonium chloride). Methods: Acrylic resin specimens with and without surface treatment were subjected to tests for fungistatic and fungicidal activities. Standard strains of Candida albicans and Aspergillus niger were used. Results: After surface treatment, the fungistatic and fungicidal efficacies of the resins against C. albicans and fungistatic action against A. niger were verified. Conclusions: The surface treatment was a determinant of the antifungal activity of the material.

Journal of Advances in Biology & Biotechnology

The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated fro... more The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possi...