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Papers by Robert Good

The Journal of Immunology, Jul 1, 2006

immunologic defect of the bursectomized-irradiated chickens as 19S and 7S agalnmaglobulinemia wit... more immunologic defect of the bursectomized-irradiated chickens as 19S and 7S agalnmaglobulinemia with complete failure of specific antibody production. The thymectomized-irradiated chickens had depressed antibody production resembling that of neonataUy thymectomized mice and rats.

Canadian Medical Association Journal, Apr 16, 1966

Phenylketonuria and severe focal scleroderma were observed in a white male child. This is the fir... more Phenylketonuria and severe focal scleroderma were observed in a white male child. This is the first instance in which the association of these two rare disorders has been reported. Studies carried out on this patient provide a possible explanation for the abnormalities of indole metabolism in phenylketonuria. On an unrestricted diet, when serum phenylalanine levels were elevated, excessive urinary excretion of indolic tryptophan metabolites was seen 18-24 hours after oral tryptophan loading, and tryptophan was demonstrable in the stool. This was not observed when the serum phenylalanine was within normal limits on a low phenylalanine diet. Impaired intestinal tryptophan absorption secondary to elevated serum phenylalanine, by providing tryptophan substrate for bacterial degradation to indolic compounds which are absorbed and excreted in the urine, may partially explain the abnormalities of indole metabolism in phenylketonuria.

Clinical Immunology and Immunopathology, 1979

Abstract Human T cell subpopulations (Tμ and Tγ) were examined for their distribution in the peri... more Abstract Human T cell subpopulations (Tμ and Tγ) were examined for their distribution in the peripheral blood, cord blood, bone marrow, tonsils, thymus, lymph nodes and spleen. The proportions of Tμ and Tγ cells are comparable in the peripheral blood, tonsils and bone ...

Infection and Immunity, 1972

J Allerg Clin Immunol, 1978

The Journal of Immunology, Sep 1, 1993

We have previously shown that a synthetic peptide (CKS-17) homologous to retroviral envelope prot... more We have previously shown that a synthetic peptide (CKS-17) homologous to retroviral envelope protein suppresses the accumulation of superantigen staphylococcal enterotoxin-induced TNF-alpha mRNA in human PBMC and in highly purified human monocytes. The present study was designed to examine the underlying mechanism(s) by which CKS-17 down-regulates the TNF-alpha mRNA expression using a human acute monocytic leukemia cell line THP-1 stimulated with the superantigen staphylococcal enterotoxin E. A cyclooxygenase inhibitor indomethacin does not reverse the inhibition of TNF-alpha mRNA expression by CKS-17, suggesting that prostaglandins are not responsible for the suppressive action of CKS-17. The inhibitory effect of CKS-17 is, however, significantly blocked by a protein synthesis inhibitor cycloheximide, indicating that CKS-17 requires de novo protein synthesis to induce the suppressive activity. The mRNA stability assays using actinomycin D show that CKS-17 does not decrease the TNF-alpha mRNA stability. Nuclear run-on transcription assays further reveal that CKS-17 suppresses the TNF-alpha mRNA transcription rate. Taken together, these results suggest that the synthetic retroviral peptide CKS-17 down-regulates TNF-alpha mRNA expression through inhibition of transcriptional activation of the TNF-alpha gene, which requires de novo synthesis of a transcriptional repressor protein(s).

N Engl J Med, 1969

... Copenhagen, Munksgaard, 1968, pp 117-122 32. ... A., 189 Hong, R., 205 Hosking, CS, 78 Inamda... more ... Copenhagen, Munksgaard, 1968, pp 117-122 32. ... A., 189 Hong, R., 205 Hosking, CS, 78 Inamdar, Savita, 126 Johnsen, Stanley D., 183 Jose, David G., 198 Kersey, John H., 198 Kokubu, NL, 23 Kretschmer, RR, 88 Lamenza, C, 23 Levy, Arthur L., 61 Lewis-McKinley, C, 23 ...

Int J Immunopharmacol, 1991

We studied the tolerance, acceptance and immunologic effects of hydrolytic enzymes as BRM in asym... more We studied the tolerance, acceptance and immunologic effects of hydrolytic enzymes as BRM in asymptomatic HIV-I carriers.

J Pediat, 1967

The ]ournal of PEDIATRICS 645

Proceedings of the Society For Experimental Biology and Medicine, May 1, 1992

Suppression of immune functions was demonstrated in both humans and animals when exogenous RNA wa... more Suppression of immune functions was demonstrated in both humans and animals when exogenous RNA was eliminated from the diet. However, direct actions of RNA/nucleotide on the immune system are virtually unknown. Thus, in this study, we explored effects of RNA and nucleotide on lymphocyte functions in vitro. Yeast whole RNA, which is free of endotoxin, was supplemented to culture media, and changes in mitogen responses, thymocyte proliferation, or in vitro antibody production by murine spleen lymphocytes were analyzed. Yeast whole RNA potentiated the proliferation of spleen lymphocytes and it also strikingly enhanced in vitro antibody production in response to sheep red blood cells at least 10-fold. However, it did not potentiate the proliferation of thymocytes (immature lymphocytes). These enhancing activities of yeast RNA were significantly reduced by RNAse treatment, but not by treatments with DNAse or polymyxin B. Certain mononucleotides exhibited less, but similar, action on murine spleen lymphocytes. The whole yeast RNA employed was already degraded to small nucleotide (less than 1 kb). Therefore, it may be suggested that certain components of RNA degraders can function as powerful immunomodulators, indicating that exogenous RNA or nucleotide may be important in facilitating immune responses under certain circumstances.

Cell Immunol, 1981

... 331337. Academic Press, New York, 1975. 4. Brummerstedt, E., Flagstad, T., Basse, A., andAndr... more ... 331337. Academic Press, New York, 1975. 4. Brummerstedt, E., Flagstad, T., Basse, A., andAndresen, E., Ada Pathol. Microbiol. Scand. (A) 79,686,1971. ... 34. Martell, AE, and Calvin, M., "Chemistry of the Metal Chelate Compounds." PrenticeHall, Englewood Cliffs, NJ, 1952. 35. ...

The Journal of Immunology, Mar 1, 1976

The influence of a low protein (6%) diet on the immunologic function of NZB mice was investigated... more The influence of a low protein (6%) diet on the immunologic function of NZB mice was investigated. The low protein intake was associated with decreased weight gain in both male and female NZB mice. The mice fed the low protein diet did not develop splenomegaly, which generally occurs by 7 to 10 months of age in NZB mice fed a normal amount of protein. Further, 7- to 10-month-old NZB mice fed the low protein(6%) diet, maintained: 1) more vigorous antibody production to sheep red blood cells; 2) greater capacity to produce graft-vs-host reactions, and 3) more vigorous cell-mediated &amp;quot;killer&amp;quot; cell immunity after immunization against DBA/2 mastocytoma cells than did NZB mice on a normal (22%) protein diet. The decrease of PHA and Con A response which normally occurs with aging in NZB mice was abrogated to some degree by protein restriction. However, response to LPS, which also declines with age in NZB mice, did not appear to be influenced by diet.

Proceedings of the National Academy of Sciences of the United States of America, Apr 1, 1997

HIV-1 Nef protein shares a significant homology with the immunosuppressive and highly conserved r... more HIV-1 Nef protein shares a significant homology with the immunosuppressive and highly conserved retroviral transmembrane protein p15E. In the present study, extracellular Nef protein is shown to induce interleukin (IL)-10 mRNA expression in human peripheral blood mononuclear cells as well as in cells of H9 T and U937 promonocytic human cell lines. Release of IL-10 protein into supernatants of peripheral blood mononuclear cells stimulated with Nef is dose-dependent. Expression of cytokines IL-2, IL-4, IL-5, IL-12 p40, IL-13, and interferon γ is not affected by Nef stimulation. IL-10 protein production induced by Nef is inhibited by the calcium/calmodulin phosphodiesterase inhibitor W-7 but not by the protein kinase A inhibitor H-89 nor the protein kinase C inhibitors staurosporine and calphostin C. The calcium chelating agent EGTA also inhibits the IL-10 production induced by Nef, and this inhibition is reversed by the addition of calcium along with Nef. These findings indicate that extracellular Nef may contribute to the immunopathogenesis of HIV infection by inducing IL-10.

Cancer Research, Jun 1, 1966

There are 2 pathways of normal lymphoid differentiation. The thymus is responsible for initiation... more There are 2 pathways of normal lymphoid differentiation. The thymus is responsible for initiation and control of cellular differ entiation directed toward the small lymphocyte and cellular immunity. The avian bursa of Fabricius, and perhaps Peyer's patches of man, initiates and controls another lymphoid differ entiation pathway directed toward the plasma cell and immunoglobulin production. Thymus-dependent and bursa-dependent lymphocytic malignancies have been defined in the mouse and chicken, respectively, and are featured by abnormal Lymphoid differentiation beginning in one or the other central lymphoid organ. It is proposed that clinical lymphoid malignancies may also be usefully classified as either thymus system or immunoglobulin-producing system malignancies. In this view Hodgkin's disease appears to be a thymus system disease, and more focused study of its central organ seems indicated.

Cancer Research, Feb 1, 1982

The destructive effects of malnutrition on healthy bodily functioning have been recognized throug... more The destructive effects of malnutrition on healthy bodily functioning have been recognized throughout history. Recent scientific inquiries have begun to analyze the precise relation ships between various nutritional factors and immunological function and the consequences of these relationships for pathogenetic processes involving several of the major organ sys tems. Strikingly different biological relationships have been revealed.