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Papers by Robert Matson
Applying Genomic and Proteomic Microarray Technology in Drug Discovery, 2004
SOJ Biochemistry, 2016
Open Access Research Article prepared extracts of various allergens onto a nitrocellulose slide (... more Open Access Research Article prepared extracts of various allergens onto a nitrocellulose slide (FAST Slide, Schleicher & Schull). The presence of sIgE In sera For Allergens (Dermatophagoides pteronyssinus (Dp), Egg White, Soybean, Milk, and Wheat) was determined by immune fluorescence laser scanning of the microarray. The microarray results correlated well with those determined using UniCap (Pharmacia & Upjohn Diagnostics AB). Bacarese-Hamilton, [3] prepared allergen microarrays from extracts onto activated glass slides and evaluated sensitivity and specificity using the Tyramide Signal Amplification (TSA) catalyzed by HRP (Molecular Probes). A lower limit of detection of the microarray bound sIgE < 1 fg was achieved from 100 µl sera corresponding to about 10 fg/ mL equivalent to ~ 4.1 X 10-6 IU/ mL (1 IU/ mL IgE = 2.44 ng/ mL) or a cutoff value at 4.1 k IU/ L. Lebrun, [4] adapted the standard ELISA for quantitative determinations on a nitrocellulose substrate (Zeta-Grip, Miragene) using a flat-bed scanner for colorimetric detection of the developed microarray. Sensitivities for sIgE were achieved that fell below the World Health Organization (WHO) cutoff value of 0.35 k IU/ L. Hiller, et al. [5] first immobilized purified or recombinant allergenic proteins (rather than crude extracts) onto amine reactive glass slides (VBC-Genomics, Vienna) to measure allergen sIgE. This approach subsequently led to the co-development with Phadia of the ISAC (Immuno Solid-phase Allergen Chip) microarray ushering in Component-Resolved Diagnostic (CRD) for allergen testing. The ImmunoCAP ISAC chip products are commercially available from Thermo Fisher Scientific for the assessment of sIgE. Ott, et al. [6] used purified allergenic proteins from milk and eggs spotted onto a microarray slide in an effort to assess the clinical predictive value of component resolved diagnosis. The study encompassed 130 children with allergy to milk or eggs and included correlation of the microarray to that of UniCap (Phadia). The authors concluded, " that microarray-based IgE
Lipids, 1981
The accumulation of (1-palmitoyl)lysophosphatidylcholine, lysolecithin, in gallbladder bile was o... more The accumulation of (1-palmitoyl)lysophosphatidylcholine, lysolecithin, in gallbladder bile was observed during the first week of cholesterol-induced experimentals cholelithiasis using the prairie dog model for cholesterol gallstone formation. Gallbladder fluid transport function decreased as bile lysolecithin concentration increased. These observations suggest that lysolecithin play an important, early role in the etiology of gallstone disease. Furthermore, the relative activities of hepatic and
Applying Genomic and Proteomic Microarray Technology in Drug Discovery, 2004
Open Forum Infectious Diseases
Applying Genomic and Proteomic Microarray Technology in Drug Discovery, 2004
SOJ Biochemistry, 2016
Open Access Research Article prepared extracts of various allergens onto a nitrocellulose slide (... more Open Access Research Article prepared extracts of various allergens onto a nitrocellulose slide (FAST Slide, Schleicher & Schull). The presence of sIgE In sera For Allergens (Dermatophagoides pteronyssinus (Dp), Egg White, Soybean, Milk, and Wheat) was determined by immune fluorescence laser scanning of the microarray. The microarray results correlated well with those determined using UniCap (Pharmacia & Upjohn Diagnostics AB). Bacarese-Hamilton, [3] prepared allergen microarrays from extracts onto activated glass slides and evaluated sensitivity and specificity using the Tyramide Signal Amplification (TSA) catalyzed by HRP (Molecular Probes). A lower limit of detection of the microarray bound sIgE < 1 fg was achieved from 100 µl sera corresponding to about 10 fg/ mL equivalent to ~ 4.1 X 10-6 IU/ mL (1 IU/ mL IgE = 2.44 ng/ mL) or a cutoff value at 4.1 k IU/ L. Lebrun, [4] adapted the standard ELISA for quantitative determinations on a nitrocellulose substrate (Zeta-Grip, Miragene) using a flat-bed scanner for colorimetric detection of the developed microarray. Sensitivities for sIgE were achieved that fell below the World Health Organization (WHO) cutoff value of 0.35 k IU/ L. Hiller, et al. [5] first immobilized purified or recombinant allergenic proteins (rather than crude extracts) onto amine reactive glass slides (VBC-Genomics, Vienna) to measure allergen sIgE. This approach subsequently led to the co-development with Phadia of the ISAC (Immuno Solid-phase Allergen Chip) microarray ushering in Component-Resolved Diagnostic (CRD) for allergen testing. The ImmunoCAP ISAC chip products are commercially available from Thermo Fisher Scientific for the assessment of sIgE. Ott, et al. [6] used purified allergenic proteins from milk and eggs spotted onto a microarray slide in an effort to assess the clinical predictive value of component resolved diagnosis. The study encompassed 130 children with allergy to milk or eggs and included correlation of the microarray to that of UniCap (Phadia). The authors concluded, " that microarray-based IgE
Lipids, 1981
The accumulation of (1-palmitoyl)lysophosphatidylcholine, lysolecithin, in gallbladder bile was o... more The accumulation of (1-palmitoyl)lysophosphatidylcholine, lysolecithin, in gallbladder bile was observed during the first week of cholesterol-induced experimentals cholelithiasis using the prairie dog model for cholesterol gallstone formation. Gallbladder fluid transport function decreased as bile lysolecithin concentration increased. These observations suggest that lysolecithin play an important, early role in the etiology of gallstone disease. Furthermore, the relative activities of hepatic and
Applying Genomic and Proteomic Microarray Technology in Drug Discovery, 2004
Open Forum Infectious Diseases