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Papers by Robert Pinard
Nucleic acids …, 1994
Using a conditional expression system with the temperature-induclble XP L promoter, we previously... more Using a conditional expression system with the temperature-induclble XP L promoter, we previously showed that the single mutations 13U-A and 914A-U, and the double mutation 13U-A and 914A-U In Escherichia coll 16S ribosomal RNA Impair the binding of streptomycin (Pinard et at., The FASEB Journal, 1993, 7,173-176). In this study, we found that the two single mutations and the double mutation increase translational fidelity, reducing In vivo readthrough of nonsense codons and frameshifting, and decreasing In vitro mislncorporatlon In a poly(U)-dlrected system. Using ollgodeoxyribonucleotlde probes which hybridize to the 530 loop and to the 1400 region of 16S rRNA, two regions Involved In the control of tRNA binding to the A site, we observed that the mutations In rRNA Increase the binding of the probe to the 530 loop but not to the 1400 region. We suggest that the mutations at positions 13 and 914 of 16S rRNA induce a conformatlonal rearrangement In the 530 loop, which contributes to the Increased accuracy of the ribosome.
Nucleic acids …, 1994
Using a conditional expression system with the temperature-induclble XP L promoter, we previously... more Using a conditional expression system with the temperature-induclble XP L promoter, we previously showed that the single mutations 13U-A and 914A-U, and the double mutation 13U-A and 914A-U In Escherichia coll 16S ribosomal RNA Impair the binding of streptomycin (Pinard et at., The FASEB Journal, 1993, 7,173-176). In this study, we found that the two single mutations and the double mutation increase translational fidelity, reducing In vivo readthrough of nonsense codons and frameshifting, and decreasing In vitro mislncorporatlon In a poly(U)-dlrected system. Using ollgodeoxyribonucleotlde probes which hybridize to the 530 loop and to the 1400 region of 16S rRNA, two regions Involved In the control of tRNA binding to the A site, we observed that the mutations In rRNA Increase the binding of the probe to the 530 loop but not to the 1400 region. We suggest that the mutations at positions 13 and 914 of 16S rRNA induce a conformatlonal rearrangement In the 530 loop, which contributes to the Increased accuracy of the ribosome.