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Papers by Roberto Luchetti

Research paper thumbnail of Long term outcome in ulcerative colitis patients with corticosteroids-induced remission

Long term outcome in ulcerative colitis patients with corticosteroids-induced remission

Digestion

Research paper thumbnail of L’anemia nelle malattie infiammatorie croniche dell’intestino

L’anemia nelle malattie infiammatorie croniche dell’intestino

Research paper thumbnail of Emorragia intramurale dell’intestino tenue come manifestazione di Celiachia misconosciuta

Emorragia intramurale dell’intestino tenue come manifestazione di Celiachia misconosciuta

Research paper thumbnail of Giovannini C, Luchetti R, Mancini E, De Vincenzi M. Effects of cereal prolamin peptides on differentiated CaCo-2 cells. Alternatives to laboratory animals: ATLA 1996;24:547-552

Giovannini C, Luchetti R, Mancini E, De Vincenzi M. Effects of cereal prolamin peptides on differentiated CaCo-2 cells. Alternatives to laboratory animals: ATLA 1996;24:547-552

Alternatives to laboratory animals: ATLA

Research paper thumbnail of 38. Giovannini C, Luchetti R, De Vincenzi M. The activities of peptides "31-43", "44-55" and "56-68" of A-gliadin on in vitro cultures of CaCo-2 cells. Alternatives to laboratory animals: ATLA 1997;25:437-443

38. Giovannini C, Luchetti R, De Vincenzi M. The activities of peptides "31-43", "44-55" and "56-68" of A-gliadin on in vitro cultures of CaCo-2 cells. Alternatives to laboratory animals: ATLA 1997;25:437-443

Alternatives to laboratory animals: ATLA

Research paper thumbnail of Agglutinating activity of alcohol-soluble proteins from quinoa seed flour in celiac disease

Plant foods for human nutrition (Dordrecht, Netherlands), 1999

The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, a... more The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, after peptic-tryptic digestion, are unable to agglutinate K562(s) cells. When separated by affinity chromatography on sepharose-6B coupled with mannan, peptic-tryptic digest separated in two fractions. Fraction B peptides (about 1% of total protein) were shown to agglutinate K562(s) cells at a very low concentration, whereas peptides in fraction A and in the mixed fraction A+B were inactive, suggesting that fraction A contains protective peptides that interfere with the agglutinating activity of toxic peptides in fraction B.

Research paper thumbnail of In vitro toxicity testing of alcohol-soluble proteins from diploid wheat triticum monococcum in celiac disease

Journal of Biochemical Toxicology, 1996

Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Triticum monoc... more Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Triticum monococcum with contrasting storage protein compositions and bread-making characteristics were found unable to agglutinate K562(S) cells even at a peptide concentration as high as 14 g/L, agglutination being strongly correlated with toxicity in celiac disease. When fractionated by affinity chromatography on Sepharose-6B coupled with mannan, peptic-tryptic digests separated into three fractions. Fraction C peptides were shown to agglutinate K562(S) cells, whereas peptides in fractions A and B and in the mixed fraction B ‫‬ C were inactive, suggesting that fraction B contains "protective" peptides that interfere with toxic peptides in fraction C in their agglutinating activity. These results offer an opportunity to study the biochemical and genetic bases of wheat toxicity at the diploid level. Moreover, the reduced toxicity, if any, of Triticum monococcum in the celiac disease, along with the good grain characteristics of some "monococcum" accessions, greatly increases the economical prospects of this wheat species.

Research paper thumbnail of In vitro assessment of acetic‐acid‐soluble proteins (glutenin) toxicity in celiac disease

Journal of Biochemical Toxicology, 1996

Acetic-acid-soluble storage proteins from gluten of the bread wheat cv. Sprint 3 were fractionate... more Acetic-acid-soluble storage proteins from gluten of the bread wheat cv. Sprint 3 were fractionated by adsorption chromatography on 2000 Å controlledpore glass (CPG) beads, and glutenin polymers with molecular mass higher than 10 7 Da and free from monomeric gliadins were recovered. The glutenin polymers were found to consist of high-molecular-weight (HMW) and low-molecular-weight (LMW) glutenin subunits. Peptic-tryptic (PT) digests of glutenins were examined for their agglutination activity on human myelogenous leukemia K 562(S) cells, agglutination being strongly correlated with toxicity for the celiac intestine. The peptide fraction at a concentration of 1 g/L of culture medium was able to agglutinate 30% of K 562(S) cells, suggesting a moderate toxic effect. This toxicity may be accounted for by homologies in amino acid sequences between glutenin subunits and ␣/band c-gliadins. ᭧ 1997 John Wiley & Sons, Inc.

Research paper thumbnail of Atypical Multiple Ileum Metastases of Renal Cell Carcinoma

Atypical Multiple Ileum Metastases of Renal Cell Carcinoma

Journal of Gastrointestinal Cancer, 2013

Research paper thumbnail of M1257 Rifaximin® Reduces Symptoms and Complications in Patients With Symptomatic Diverticular Disease (SDD): A Meta-Analysis

M1257 Rifaximin® Reduces Symptoms and Complications in Patients With Symptomatic Diverticular Disease (SDD): A Meta-Analysis

Gastroenterology, 2010

Research paper thumbnail of Effects on gastric emptying, orocecal transit time (OCTT), gas production and abdominal symptoms of guar gum (GG), ispaghula (I) and mycrocristalline cellulose (MC) on healthy volunteers

Effects on gastric emptying, orocecal transit time (OCTT), gas production and abdominal symptoms of guar gum (GG), ispaghula (I) and mycrocristalline cellulose (MC) on healthy volunteers

Gastroenterology, 2000

Research paper thumbnail of Antipyrine metabolism as predictor of response to r-interferon in chronic C hepatitis patients

Gastroenterology, 1995

HY Apolipoprotein (apo) B, the major protein component of atherogenic lipuproteins, occurs in two... more HY Apolipoprotein (apo) B, the major protein component of atherogenic lipuproteins, occurs in two forms, apo B100 and apo B48 that are expressed from the same gene by posttranscriptional mRNA editing. An enzyme-catalyzed site-specific base change from C to U introduces a premature stop codon within the mRNA, resulting in the formation of the truncated form, apo B48. In several mammalian species, including horse, dog, rat and mouse, apo B mRNA editing takes place in both the intestine and the liver, resulting in low plasma levels of apo B 100-containing lipoproteins that give rise to LDL. In contrast, human, monkey and rabbit livers do not edit the apo B mRNA, therefore, these species have high LDL levels.

Research paper thumbnail of Supplementation of microcrystalline cellulose to a solid meal causes less symptoms than other dietary fibers. A study on dietary fibers physiological effects on human gut

Supplementation of microcrystalline cellulose to a solid meal causes less symptoms than other dietary fibers. A study on dietary fibers physiological effects on human gut

Digestive and Liver Disease, 2000

Research paper thumbnail of Budesonide is less effective than conventional corticosteroids in the treatment of Crohn's disease. A meta-analysis

Budesonide is less effective than conventional corticosteroids in the treatment of Crohn's disease. A meta-analysis

Digestive and Liver Disease, 2000

Research paper thumbnail of PO.34 JEJUNAL GIST. AN UNUSUAL CAUSE OF GASTROINTESTINAL BLEEDING: A CASE REPORT

Digestive and Liver Disease, 2008

Research paper thumbnail of T-cell response to different cultivars of farro wheat, Triticum turgidum ssp. dicoccum, in celiac disease patients

Research paper thumbnail of Budesonide in the treatment of Crohn's disease: a meta-analysis

Alimentary Pharmacology and Therapeutics, 2000

Research paper thumbnail of Colectomy rate in acute severe ulcerative colitis in the infliximab era

Digestive and Liver Disease, 2008

Severe ulcerative colitis is a potentially life-threatening condition. Due to advances in medical... more Severe ulcerative colitis is a potentially life-threatening condition. Due to advances in medical therapy, the mortality rate has dropped to <2% over the past 30 years, but the colectomy rate reaches 30%. Recently, infliximab has been shown to be effective as rescue therapy but little is known about long-term benefits.

Research paper thumbnail of Long term outcome in ulcerative colitis patients with corticosteroids-induced remission

Long term outcome in ulcerative colitis patients with corticosteroids-induced remission

Digestion

Research paper thumbnail of L’anemia nelle malattie infiammatorie croniche dell’intestino

L’anemia nelle malattie infiammatorie croniche dell’intestino

Research paper thumbnail of Emorragia intramurale dell’intestino tenue come manifestazione di Celiachia misconosciuta

Emorragia intramurale dell’intestino tenue come manifestazione di Celiachia misconosciuta

Research paper thumbnail of Giovannini C, Luchetti R, Mancini E, De Vincenzi M. Effects of cereal prolamin peptides on differentiated CaCo-2 cells. Alternatives to laboratory animals: ATLA 1996;24:547-552

Giovannini C, Luchetti R, Mancini E, De Vincenzi M. Effects of cereal prolamin peptides on differentiated CaCo-2 cells. Alternatives to laboratory animals: ATLA 1996;24:547-552

Alternatives to laboratory animals: ATLA

Research paper thumbnail of 38. Giovannini C, Luchetti R, De Vincenzi M. The activities of peptides "31-43", "44-55" and "56-68" of A-gliadin on in vitro cultures of CaCo-2 cells. Alternatives to laboratory animals: ATLA 1997;25:437-443

38. Giovannini C, Luchetti R, De Vincenzi M. The activities of peptides "31-43", "44-55" and "56-68" of A-gliadin on in vitro cultures of CaCo-2 cells. Alternatives to laboratory animals: ATLA 1997;25:437-443

Alternatives to laboratory animals: ATLA

Research paper thumbnail of Agglutinating activity of alcohol-soluble proteins from quinoa seed flour in celiac disease

Plant foods for human nutrition (Dordrecht, Netherlands), 1999

The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, a... more The edible seeds of the quinoa plant contain small quantities of alcohol-soluble protein which, after peptic-tryptic digestion, are unable to agglutinate K562(s) cells. When separated by affinity chromatography on sepharose-6B coupled with mannan, peptic-tryptic digest separated in two fractions. Fraction B peptides (about 1% of total protein) were shown to agglutinate K562(s) cells at a very low concentration, whereas peptides in fraction A and in the mixed fraction A+B were inactive, suggesting that fraction A contains protective peptides that interfere with the agglutinating activity of toxic peptides in fraction B.

Research paper thumbnail of In vitro toxicity testing of alcohol-soluble proteins from diploid wheat triticum monococcum in celiac disease

Journal of Biochemical Toxicology, 1996

Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Triticum monoc... more Peptic-tryptic digests of alcohol-soluble proteins from flours of 10 accessions of Triticum monococcum with contrasting storage protein compositions and bread-making characteristics were found unable to agglutinate K562(S) cells even at a peptide concentration as high as 14 g/L, agglutination being strongly correlated with toxicity in celiac disease. When fractionated by affinity chromatography on Sepharose-6B coupled with mannan, peptic-tryptic digests separated into three fractions. Fraction C peptides were shown to agglutinate K562(S) cells, whereas peptides in fractions A and B and in the mixed fraction B ‫‬ C were inactive, suggesting that fraction B contains "protective" peptides that interfere with toxic peptides in fraction C in their agglutinating activity. These results offer an opportunity to study the biochemical and genetic bases of wheat toxicity at the diploid level. Moreover, the reduced toxicity, if any, of Triticum monococcum in the celiac disease, along with the good grain characteristics of some "monococcum" accessions, greatly increases the economical prospects of this wheat species.

Research paper thumbnail of In vitro assessment of acetic‐acid‐soluble proteins (glutenin) toxicity in celiac disease

Journal of Biochemical Toxicology, 1996

Acetic-acid-soluble storage proteins from gluten of the bread wheat cv. Sprint 3 were fractionate... more Acetic-acid-soluble storage proteins from gluten of the bread wheat cv. Sprint 3 were fractionated by adsorption chromatography on 2000 Å controlledpore glass (CPG) beads, and glutenin polymers with molecular mass higher than 10 7 Da and free from monomeric gliadins were recovered. The glutenin polymers were found to consist of high-molecular-weight (HMW) and low-molecular-weight (LMW) glutenin subunits. Peptic-tryptic (PT) digests of glutenins were examined for their agglutination activity on human myelogenous leukemia K 562(S) cells, agglutination being strongly correlated with toxicity for the celiac intestine. The peptide fraction at a concentration of 1 g/L of culture medium was able to agglutinate 30% of K 562(S) cells, suggesting a moderate toxic effect. This toxicity may be accounted for by homologies in amino acid sequences between glutenin subunits and ␣/band c-gliadins. ᭧ 1997 John Wiley & Sons, Inc.

Research paper thumbnail of Atypical Multiple Ileum Metastases of Renal Cell Carcinoma

Atypical Multiple Ileum Metastases of Renal Cell Carcinoma

Journal of Gastrointestinal Cancer, 2013

Research paper thumbnail of M1257 Rifaximin® Reduces Symptoms and Complications in Patients With Symptomatic Diverticular Disease (SDD): A Meta-Analysis

M1257 Rifaximin® Reduces Symptoms and Complications in Patients With Symptomatic Diverticular Disease (SDD): A Meta-Analysis

Gastroenterology, 2010

Research paper thumbnail of Effects on gastric emptying, orocecal transit time (OCTT), gas production and abdominal symptoms of guar gum (GG), ispaghula (I) and mycrocristalline cellulose (MC) on healthy volunteers

Effects on gastric emptying, orocecal transit time (OCTT), gas production and abdominal symptoms of guar gum (GG), ispaghula (I) and mycrocristalline cellulose (MC) on healthy volunteers

Gastroenterology, 2000

Research paper thumbnail of Antipyrine metabolism as predictor of response to r-interferon in chronic C hepatitis patients

Gastroenterology, 1995

HY Apolipoprotein (apo) B, the major protein component of atherogenic lipuproteins, occurs in two... more HY Apolipoprotein (apo) B, the major protein component of atherogenic lipuproteins, occurs in two forms, apo B100 and apo B48 that are expressed from the same gene by posttranscriptional mRNA editing. An enzyme-catalyzed site-specific base change from C to U introduces a premature stop codon within the mRNA, resulting in the formation of the truncated form, apo B48. In several mammalian species, including horse, dog, rat and mouse, apo B mRNA editing takes place in both the intestine and the liver, resulting in low plasma levels of apo B 100-containing lipoproteins that give rise to LDL. In contrast, human, monkey and rabbit livers do not edit the apo B mRNA, therefore, these species have high LDL levels.

Research paper thumbnail of Supplementation of microcrystalline cellulose to a solid meal causes less symptoms than other dietary fibers. A study on dietary fibers physiological effects on human gut

Supplementation of microcrystalline cellulose to a solid meal causes less symptoms than other dietary fibers. A study on dietary fibers physiological effects on human gut

Digestive and Liver Disease, 2000

Research paper thumbnail of Budesonide is less effective than conventional corticosteroids in the treatment of Crohn's disease. A meta-analysis

Budesonide is less effective than conventional corticosteroids in the treatment of Crohn's disease. A meta-analysis

Digestive and Liver Disease, 2000

Research paper thumbnail of PO.34 JEJUNAL GIST. AN UNUSUAL CAUSE OF GASTROINTESTINAL BLEEDING: A CASE REPORT

Digestive and Liver Disease, 2008

Research paper thumbnail of T-cell response to different cultivars of farro wheat, Triticum turgidum ssp. dicoccum, in celiac disease patients

Research paper thumbnail of Budesonide in the treatment of Crohn's disease: a meta-analysis

Alimentary Pharmacology and Therapeutics, 2000

Research paper thumbnail of Colectomy rate in acute severe ulcerative colitis in the infliximab era

Digestive and Liver Disease, 2008

Severe ulcerative colitis is a potentially life-threatening condition. Due to advances in medical... more Severe ulcerative colitis is a potentially life-threatening condition. Due to advances in medical therapy, the mortality rate has dropped to <2% over the past 30 years, but the colectomy rate reaches 30%. Recently, infliximab has been shown to be effective as rescue therapy but little is known about long-term benefits.