Robiul Fuadi - Academia.edu (original) (raw)

Papers by Robiul Fuadi

Research paper thumbnail of Effect of MTHFR A1298C Gene Polymorphism on Acute Coronary Syndrome

PubMed, Feb 1, 2023

Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Acute coron... more Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Acute coronary syndrome is a manifestation of CVD. In Indonesia, limited studies have been conducted on genetics as a potential risk factor for acute coronary syndrome (ACS). Consequently, this study aimed to examine the effect of the methylenetetrahydrofolate reductase (MTHFR) A1298C gene polymorphism on the incidence of ACS. Method: The study employed a case-control design. Outpatients from the cardiology and internal medicine clinics at the University of Airlangga (UNAIR) Hospital in Surabaya, Indonesia, constituted the study population. The case group comprised 60 patients with a history of ACS, while the control group consisted of 30 patients without a history of cardiovascular complaints. MTHFR A12980C gene polymorphism examination was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP) method at the Tropical Disease Center UNAIR Laboratory. Results: Among the ACS group, 29 (48.1%), 13 (21.7%), and 18 (30%) of the individuals had AA, AC, and CC genotype patterns, respectively. In the control group, 16 individuals had AA (53.3%), 6 AC (20%), and 8 CC (26.7%). The C allele variant was identified in 41% of the ACS group and 37% of the control group. The odds ratio (OR) for the incidence of ACS was 1.195 (95% confidence interval [CI]; 0.381-3.752), 1.241 (95% CI; 0.481-3.486), and 1.222 (95% CI; 0.381-3.752). Chi-square analysis revealed no association between MTHFR A1298C gene polymorphism and the incidence of ACS (P > 0.05). Conclusions: MTHFR A1298C gene polymorphism did not significantly affect ACS incidence.

Research paper thumbnail of Differences in Glutamate Dehydrogenase (GLDH) and Other Liver Biochemistry Levels before and after Remdesivir Treatment in COVID-19

Research journal of pharmacy and technology, Apr 22, 2024

Research paper thumbnail of Massive subcutaneous abscess: A case report of management and source control

International journal of surgery case reports, May 1, 2024

Research paper thumbnail of Association between Homocysteine Level and Vitamin D Deficiency with Acute Coronary Syndrome in Indonesia

Research journal of pharmacy and technology, Feb 20, 2024

Research paper thumbnail of Role of MMP-9, TIMP-1 as markers of lung imaging and functional abnormality of COVID-19

Annals of Medicine and Surgery, Dec 3, 2023

Research paper thumbnail of Korelasi Cara Pemeriksaan Sedimen Urine Eritrosit Dan Leukosit Secara Kuantitatif Dengan Metode Flowcytometry Dibandingkan Metode Shih-Yung

Research paper thumbnail of Effect of Dyslipidemia Therapy on Creatinine Kinase Activity Level in Patients with Heart Disease

Indonesian Journal of Clinical Pathology and Medical Laboratory, Apr 15, 2021

Cardiovascular disease remains a significant health problem in the Asia Pacific region. Several s... more Cardiovascular disease remains a significant health problem in the Asia Pacific region. Several studies have found that dyslipidemia is a cause of morbidity and mortality and requires high medical costs. Dyslipidemia is a risk factor for atherosclerosis. The most widely used therapy for dyslipidemia is statins. Statins often cause muscle disorders such as myalgia, myopathy, and rhabdomyolysis, which can cause death. A prospective cohort study design was carried out at

Research paper thumbnail of Relationship between Serum Dehydroepiandrosterone Levels and Heart Ejection Fraction in Heart Failure Patients

Indonesian Journal of Clinical Pathology and Medical Laboratory, Apr 15, 2021

Cardiovascular disease is still a serious problem in the world of health. Life expectancy after b... more Cardiovascular disease is still a serious problem in the world of health. Life expectancy after being diagnosed with heart failure is 50% and 10% for 5 and 10 years. Steroid hormones such as Dehydroepiandrosterone (DHEAS) have cardioprotective effects by inhibiting the formation of atherosclerotic plaque, pulmonary artery vasodilators, and protecting cardiomyocytes. DHEAS levels decrease with age. Decreased DHEAS levels are associated with an increased risk of cardiovascular disease. This study aimed to know the relationship between DHEAS levels in serum and ejection fractions in heart failure patients. This cross-sectional study used a sample of 34 people aged > 30 years who had been diagnosed with heart failure by a specialist in the Department of Cardiology and Vascular Medicine. The diagnosis of heart failure uses Echocardiography to determine the ejection heart fraction. DHEAS levels were taken from venous blood and examined using the CLEIA method with an IMMULITE device (Siemens Healthineers, Germany). Statistical analysis was performed by Spearman correlation test, with a significance level of p < 0.05. Thirty-four research subjects found that 13 patients had an ejection fraction of 40% (Heart Failure with Reduced Ejection Fraction/HFrEF), 12 patients had an ejection fraction of 41-49% (borderline) and 9 patients had an ejection fraction of ≥ 50% (Heart Failure with Preserved Ejection Fraction/HFpEF). Spearman correlation test results obtained a correlation coefficient or r=0.357 with a value of p=0.038, which meant there was a significant relationship between DHEAS with ejection fraction (p < 0.05). The lower the DHEAS level, the ejection fraction would also be lower. Further with age, DHEAS levels get lower. The lower the DHEAS level, the lower the ejection fraction.

Research paper thumbnail of Kinetics of anti-SARS-CoV-2 responses post complete vaccination with coronavac: A prospective study in 50 health workers

Journal of Public Health Research, Jul 1, 2022

Research paper thumbnail of Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods

International Journal of Nephrology, May 27, 2022

Background. Rapid and reliable tests are essential for the diagnostic laboratory con rmation of u... more Background. Rapid and reliable tests are essential for the diagnostic laboratory con rmation of urinary tract infections (UTIs). Until now, UTI has been con rmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine ow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. is study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine ow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. e measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine ow cytometry and the Shih-Yung method. e diagnostic value was obtained from ROC analysis of urine ow cytometry and the culture. Results. A leukocyte cuto value of 87.2/μL had a sensitivity and speci city of 98.33% and 95%, respectively, and 98.33% sensitivity and 75% speci city at a bacterial cuto of 582.22/μL. Interestingly, our study identi ed strong and consistent agreement of leukocyte and bacterial parameters between urine ow cytometry and Shih-Yung (k 0.959, p < 0.001 and k 0.939, p < 0.001, respectively). Furthermore, through analyzing the dominance angle of the scattergram, a strong agreement was obtained with the culture result (k 0.880, p < 0.001). Conclusions. e Shih-Yung method showed consistent agreement with urine ow cytometry for the detection of leukocytes and bacteria. e use of certain cuto s for bacterial and leukocyte parameters in urine ow cytometry demonstrated very good performance in detecting acquired symptomatic UTIs.

Research paper thumbnail of Profile of Major Risk Factors in Acute Coronary Syndrome (ACS) at Pusat Pelayanan Jantung Terpadu (PPJT) Dr. Soetomo Public Hospital Surabaya Between the Period of January-December 2019

Cardiovascular and Cardiometabolic Journal (CCJ), 2021

Abstract: Background: Coronary heart disease (CHD) is a leading cause of death worldwide. One typ... more Abstract: Background: Coronary heart disease (CHD) is a leading cause of death worldwide. One type of CHD that most often causes clinical manifestations and death is Acute Coronary Syndrome (ACS). In 2013 the prevalence of SKA in Indonesia reached 1.5% and it is estimated that it will continue to increase every year. Objective: This study aims to determine the profile of major risk factors for ACS sufferers in the Pusat Pelayanan Jantung Terpadu (PPJT) Dr. Soetomo Public Hospital Surabaya in the period January-December 2019. Methods: This study used a retrospective descriptive method to analyze the patient's electronic medical record (e-MR). Results: Out of 623 patients diagnosed with ACS, 429 were excluded from the research. 194 patients who met the inclusion criteria were studied with the following details: 19 APTS patients, 43 N-STEMI patients, and 132 STEMI patients. It was found that 73% of ACS patients were male, with the 55-64 years’ age group dominating by 46%. Based on ...

Research paper thumbnail of The Correlation between total lymphocyte count, hemoglobin levels, lymphocyte/leukocyte ratio (LLR), and lymphocyte/neutrophil ratio (LNR) to CD4 levels in patients with Human Immunodeficiency Virus infection at Sanglah Hospital

Bali Medical Journal, Aug 1, 2019

Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency vir... more Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency virus (HIV) infection and characterized by a progressive decrease in the immune system function which eventually leads to the development of opportunistic infections and other complications. In order to monitor the disease progression the CD4 examination and additional Complete Blood Count (CBC) were commonly used. Objectives: To determine the correlation of total lymphocyte count, hemoglobin level, lymphocyte/leukocyte ratio (LLR) and lymphocyte/ neutrophil ratio (LNR) to CD4 levels in patients with HIV infection. Methods: This a retrospective cross-sectional analytic study conducted from July to August 2017. The total lymphocyte count, hemoglobin level, lymphocyte/leukocyte ratio (LLR) and lymphocyte/neutrophil ratio (LNR) and CD4 level were measured in the study. The data normality and the correlation were analyzed using the Kolmogorov-Smirnov test and the Pearson Correlation test, respectively, performed in SPSS version 14. The p-value less than 0.05 was considered significant for the results. Results: A total of 60 samples conducted CD4 and complete blood counts (CBC) examinations during the period July-August 2017. The mean CD4 levels were 341.73 ± 243.48 cells/µL, the total leukocyte count was 6.98 ± 2.93 x 10 3 /µL, the total lymphocyte count was 2.09 ± 0.87 x 10 3 /µL, hemoglobin level 13.38 ± 2.09 g/dL, lymphocyte/ leukocyte ratio (LLR) 0.31 ± 0.11, lymphocyte/neutrophil ratio (LNR) 0.67 ± 0.40. Statistical analysis showed that data is normally distributed. Pearson correlation analysis showed that there was a significant relationship between CD4 levels with total lymphocytes counts, hemoglobin levels, RLL, RLN with correlation coefficients of 0.571 (p = 0.000), 0.324 (p = 0.012), 0.509 (p = 0.000), 0.463 (p = 0.006), respectively. There was no significant correlation between CD4 levels and total leukocytes with a correlation coefficient of 0.171 (p = 0.19). Conclusion: There is a positive correlation between CD4 levels and total lymphocytes, hemoglobin levels, RLL and RLN in patients with HIV at Sanglah Hospital.

Research paper thumbnail of Correlation of Apo E Gene Polymorphism with Recurrent Acute Coronary Syndrome

Research paper thumbnail of Implementation of Six Sigma in Glucose POCT Quality Control at Dr. Soetomo General Academic Hospital

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Point of Care Testing (POCT) is important for the examination of critically ill patients in the e... more Point of Care Testing (POCT) is important for the examination of critically ill patients in the emergency room and intensive care unit. The evaluation of the analytic quality of POCT is needed to ensure the quality of patient care at Dr. Soetomo General Academic Hospital. The purpose of this study is to evaluate the analytical quality of POCT at Dr. Soetomo General Academic Hospital. This was an observational analytical study that was done at Dr. Soetomo General Academic Hospital Surabaya in July 2017. Point of care testing quality analysis was based on 20 within run Internal Quality Control (IQC). Internal quality control data was used to calculate the mean, SD, and CV%. CV% was calculated with the following equation "CV%=(SD í— 100)/mean". Bias % = [ control mean – control true value]/control true value x 100. The sigma value was obtained from Total Error Allowance (TEa) – Bias/CV. TEa in this study was based on ISO 15197:2003. The results of the glucose examination (58 ...

Research paper thumbnail of Kinetics of anti-SARS-CoV-2 responses post complete vaccination with coronavac: A prospective study in 50 health workers

Journal of Public Health Research, Jul 1, 2022

Research paper thumbnail of SPECIFIC IGE IMMUNOBLOT METHOD IN ALLERGIC RHINITIS (IgE Spesifik Menurut Metode Imunoblot di Rinitis Alergi)

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY, 2018

Rinitis alergi merupakan penyakit bukan akibat non-infeksi yang ditemukan antara 10−30% pendudu... more Rinitis alergi merupakan penyakit bukan akibat non-infeksi yang ditemukan antara 10−30% penduduk dewasa dunia dan dapatmenyebabkan penurunan mutu kehidupan seseorang. Rinitis alergi merupakan manifestasi alergi tipe 1 atau tipe cepat yang dimediasioleh IgE. Pemeriksaan utama rinitis alergi adalah Skin Prick Test (SPT) dan IgE spesifik. Pemeriksaan IgE spesifik mempunyai kepekaandan kekhasan yang menyerupai SPT, tidak memerlukan tenaga terlatih dan menyebabkan anafilaktik. Penelitian ini untuk mengetahuiadakah kesesuaian nilai diagnostik IgE spesifik menurut metode imunoblot dengan SPT di pasien rinitis alergi dengan mengujinya.Rancangan penelitian adalah potong lintang yang dilakukan terhadap pasien yang datang di Unit Rawat Jalan THT-KL RSUDDr. Soetomo pada bulan Mei sampai dengan Oktober 2014. Pasien dikelompokkan berdasarkan diagnosis rinitis alergi dan yang nonalergidan non-infeksi serta ditetapkan secara klinis, ada riwayat alergi, pemeriksaan fisik, serta tingkat jumlah kese...

Research paper thumbnail of Potential Marker for Diagnosis and Screening of Iron Deficiency Anemia in Children

Iron Metabolism - Iron a Double‐Edged Sword [Working Title], 2022

Iron plays a role in multiple physiological functions, naming oxygen transport, gene regulation, ... more Iron plays a role in multiple physiological functions, naming oxygen transport, gene regulation, DNA synthesis, DNA repair, and brain function. Iron deficiency anemia (IDA) may happen following iron deficiency, but iron deficiency alone may cause negative impacts on the health risk of pediatric patients. The degree of iron deficiency is described by total body iron (measured by ferritin), transport iron (measured by transferrin saturation), serum iron, and other hematologic and biochemical markers. Iron deficiency anemia is a result of insufficient iron supply causing the inability to maintain normal levels of hemoglobin. The most common causes of microcytic anemia in children are iron deficiency and thalassemia minor. There are various hematologic and biochemical parameters used for screening and diagnosis of iron deficiency anemia in children, but there is no single “best” test to diagnose iron deficiency with or without anemia. The “gold standard” for identifying iron deficiency ...

Research paper thumbnail of Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods

International Journal of Nephrology

Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of ... more Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of urinary tract infections (UTIs). Until now, UTI has been confirmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine flow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. This study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine flow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. The measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine flow cytometry and the Shih-Yung method. The diagnost...

Research paper thumbnail of ANALYSIS OF THE DIFFERENCE OF COMPLETENESS OF REPORTING AND DOCUMENTATION OF LABORATORY CRITICAL VALUES PRE AND POST-INTERVENTION IN BONA WARD RSUD Dr. SOETOMO SURABAYA

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY, 2019

Completeness of laboratory critical values reporting and documentation in medical records is impo... more Completeness of laboratory critical values reporting and documentation in medical records is important for patient safety, hospital accreditation, and legality. Preliminary study in Dr. Soetomo Hospital’s ward showed the laboratory critical value report and documentation was 0% complete, 4% incomplete, and 96% undocumented. This was a quasi experimental study with one group pretest and posttest design. Technical guidance of laboratory critical values reporting and documentation in medical records and supervision were given to 26 doctors. The results evaluated were doctor knowledge and attitude towards critical value reporting, completeness of documentation in medical records, and turn around time (TAT). Reporting critical values samples number was 72 before and after the intervention. The critical values samples taken by purposive sampling. The data was analyzed using Mann-Whitney test. There were significant differences in the level of knowledge, doctor’s attitudes, and completenes...

Research paper thumbnail of Relationship between Serum Dehydroepiandrosterone Levels and Heart Ejection Fraction in Heart Failure Patients

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Cardiovascular disease is still a serious problem in the world of health. Life expectancy after b... more Cardiovascular disease is still a serious problem in the world of health. Life expectancy after being diagnosed with heartfailure is 50% and 10% for 5 and 10 years. Steroid hormones such as Dehydroepiandrosterone (DHEAS) havecardioprotective effects by inhibiting the formation of atherosclerotic plaque, pulmonary artery vasodilators, and protectingcardiomyocytes. DHEAS levels decrease with age. Decreased DHEAS levels are associated with an increased risk ofcardiovascular disease. This study aimed to know the relationship between DHEAS levels in serum and ejection fractions inheart failure patients. This cross-sectional study used a sample of 34 people aged > 30 years who had been diagnosed withheart failure by a specialist in the Department of Cardiology and Vascular Medicine. The diagnosis of heart failure usesEchocardiography to determine the ejection heart fraction. DHEAS levels were taken from venous blood and examinedusing the CLEIA method with an IMMULITE device (Siemens He...

Research paper thumbnail of Effect of MTHFR A1298C Gene Polymorphism on Acute Coronary Syndrome

PubMed, Feb 1, 2023

Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Acute coron... more Background: Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Acute coronary syndrome is a manifestation of CVD. In Indonesia, limited studies have been conducted on genetics as a potential risk factor for acute coronary syndrome (ACS). Consequently, this study aimed to examine the effect of the methylenetetrahydrofolate reductase (MTHFR) A1298C gene polymorphism on the incidence of ACS. Method: The study employed a case-control design. Outpatients from the cardiology and internal medicine clinics at the University of Airlangga (UNAIR) Hospital in Surabaya, Indonesia, constituted the study population. The case group comprised 60 patients with a history of ACS, while the control group consisted of 30 patients without a history of cardiovascular complaints. MTHFR A12980C gene polymorphism examination was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP) method at the Tropical Disease Center UNAIR Laboratory. Results: Among the ACS group, 29 (48.1%), 13 (21.7%), and 18 (30%) of the individuals had AA, AC, and CC genotype patterns, respectively. In the control group, 16 individuals had AA (53.3%), 6 AC (20%), and 8 CC (26.7%). The C allele variant was identified in 41% of the ACS group and 37% of the control group. The odds ratio (OR) for the incidence of ACS was 1.195 (95% confidence interval [CI]; 0.381-3.752), 1.241 (95% CI; 0.481-3.486), and 1.222 (95% CI; 0.381-3.752). Chi-square analysis revealed no association between MTHFR A1298C gene polymorphism and the incidence of ACS (P > 0.05). Conclusions: MTHFR A1298C gene polymorphism did not significantly affect ACS incidence.

Research paper thumbnail of Differences in Glutamate Dehydrogenase (GLDH) and Other Liver Biochemistry Levels before and after Remdesivir Treatment in COVID-19

Research journal of pharmacy and technology, Apr 22, 2024

Research paper thumbnail of Massive subcutaneous abscess: A case report of management and source control

International journal of surgery case reports, May 1, 2024

Research paper thumbnail of Association between Homocysteine Level and Vitamin D Deficiency with Acute Coronary Syndrome in Indonesia

Research journal of pharmacy and technology, Feb 20, 2024

Research paper thumbnail of Role of MMP-9, TIMP-1 as markers of lung imaging and functional abnormality of COVID-19

Annals of Medicine and Surgery, Dec 3, 2023

Research paper thumbnail of Korelasi Cara Pemeriksaan Sedimen Urine Eritrosit Dan Leukosit Secara Kuantitatif Dengan Metode Flowcytometry Dibandingkan Metode Shih-Yung

Research paper thumbnail of Effect of Dyslipidemia Therapy on Creatinine Kinase Activity Level in Patients with Heart Disease

Indonesian Journal of Clinical Pathology and Medical Laboratory, Apr 15, 2021

Cardiovascular disease remains a significant health problem in the Asia Pacific region. Several s... more Cardiovascular disease remains a significant health problem in the Asia Pacific region. Several studies have found that dyslipidemia is a cause of morbidity and mortality and requires high medical costs. Dyslipidemia is a risk factor for atherosclerosis. The most widely used therapy for dyslipidemia is statins. Statins often cause muscle disorders such as myalgia, myopathy, and rhabdomyolysis, which can cause death. A prospective cohort study design was carried out at

Research paper thumbnail of Relationship between Serum Dehydroepiandrosterone Levels and Heart Ejection Fraction in Heart Failure Patients

Indonesian Journal of Clinical Pathology and Medical Laboratory, Apr 15, 2021

Cardiovascular disease is still a serious problem in the world of health. Life expectancy after b... more Cardiovascular disease is still a serious problem in the world of health. Life expectancy after being diagnosed with heart failure is 50% and 10% for 5 and 10 years. Steroid hormones such as Dehydroepiandrosterone (DHEAS) have cardioprotective effects by inhibiting the formation of atherosclerotic plaque, pulmonary artery vasodilators, and protecting cardiomyocytes. DHEAS levels decrease with age. Decreased DHEAS levels are associated with an increased risk of cardiovascular disease. This study aimed to know the relationship between DHEAS levels in serum and ejection fractions in heart failure patients. This cross-sectional study used a sample of 34 people aged > 30 years who had been diagnosed with heart failure by a specialist in the Department of Cardiology and Vascular Medicine. The diagnosis of heart failure uses Echocardiography to determine the ejection heart fraction. DHEAS levels were taken from venous blood and examined using the CLEIA method with an IMMULITE device (Siemens Healthineers, Germany). Statistical analysis was performed by Spearman correlation test, with a significance level of p < 0.05. Thirty-four research subjects found that 13 patients had an ejection fraction of 40% (Heart Failure with Reduced Ejection Fraction/HFrEF), 12 patients had an ejection fraction of 41-49% (borderline) and 9 patients had an ejection fraction of ≥ 50% (Heart Failure with Preserved Ejection Fraction/HFpEF). Spearman correlation test results obtained a correlation coefficient or r=0.357 with a value of p=0.038, which meant there was a significant relationship between DHEAS with ejection fraction (p < 0.05). The lower the DHEAS level, the ejection fraction would also be lower. Further with age, DHEAS levels get lower. The lower the DHEAS level, the lower the ejection fraction.

Research paper thumbnail of Kinetics of anti-SARS-CoV-2 responses post complete vaccination with coronavac: A prospective study in 50 health workers

Journal of Public Health Research, Jul 1, 2022

Research paper thumbnail of Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods

International Journal of Nephrology, May 27, 2022

Background. Rapid and reliable tests are essential for the diagnostic laboratory con rmation of u... more Background. Rapid and reliable tests are essential for the diagnostic laboratory con rmation of urinary tract infections (UTIs). Until now, UTI has been con rmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine ow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. is study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine ow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. e measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine ow cytometry and the Shih-Yung method. e diagnostic value was obtained from ROC analysis of urine ow cytometry and the culture. Results. A leukocyte cuto value of 87.2/μL had a sensitivity and speci city of 98.33% and 95%, respectively, and 98.33% sensitivity and 75% speci city at a bacterial cuto of 582.22/μL. Interestingly, our study identi ed strong and consistent agreement of leukocyte and bacterial parameters between urine ow cytometry and Shih-Yung (k 0.959, p < 0.001 and k 0.939, p < 0.001, respectively). Furthermore, through analyzing the dominance angle of the scattergram, a strong agreement was obtained with the culture result (k 0.880, p < 0.001). Conclusions. e Shih-Yung method showed consistent agreement with urine ow cytometry for the detection of leukocytes and bacteria. e use of certain cuto s for bacterial and leukocyte parameters in urine ow cytometry demonstrated very good performance in detecting acquired symptomatic UTIs.

Research paper thumbnail of Profile of Major Risk Factors in Acute Coronary Syndrome (ACS) at Pusat Pelayanan Jantung Terpadu (PPJT) Dr. Soetomo Public Hospital Surabaya Between the Period of January-December 2019

Cardiovascular and Cardiometabolic Journal (CCJ), 2021

Abstract: Background: Coronary heart disease (CHD) is a leading cause of death worldwide. One typ... more Abstract: Background: Coronary heart disease (CHD) is a leading cause of death worldwide. One type of CHD that most often causes clinical manifestations and death is Acute Coronary Syndrome (ACS). In 2013 the prevalence of SKA in Indonesia reached 1.5% and it is estimated that it will continue to increase every year. Objective: This study aims to determine the profile of major risk factors for ACS sufferers in the Pusat Pelayanan Jantung Terpadu (PPJT) Dr. Soetomo Public Hospital Surabaya in the period January-December 2019. Methods: This study used a retrospective descriptive method to analyze the patient's electronic medical record (e-MR). Results: Out of 623 patients diagnosed with ACS, 429 were excluded from the research. 194 patients who met the inclusion criteria were studied with the following details: 19 APTS patients, 43 N-STEMI patients, and 132 STEMI patients. It was found that 73% of ACS patients were male, with the 55-64 years’ age group dominating by 46%. Based on ...

Research paper thumbnail of The Correlation between total lymphocyte count, hemoglobin levels, lymphocyte/leukocyte ratio (LLR), and lymphocyte/neutrophil ratio (LNR) to CD4 levels in patients with Human Immunodeficiency Virus infection at Sanglah Hospital

Bali Medical Journal, Aug 1, 2019

Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency vir... more Background: Acquired immunodeficiency syndrome (AIDS) is caused by the Human immunodeficiency virus (HIV) infection and characterized by a progressive decrease in the immune system function which eventually leads to the development of opportunistic infections and other complications. In order to monitor the disease progression the CD4 examination and additional Complete Blood Count (CBC) were commonly used. Objectives: To determine the correlation of total lymphocyte count, hemoglobin level, lymphocyte/leukocyte ratio (LLR) and lymphocyte/ neutrophil ratio (LNR) to CD4 levels in patients with HIV infection. Methods: This a retrospective cross-sectional analytic study conducted from July to August 2017. The total lymphocyte count, hemoglobin level, lymphocyte/leukocyte ratio (LLR) and lymphocyte/neutrophil ratio (LNR) and CD4 level were measured in the study. The data normality and the correlation were analyzed using the Kolmogorov-Smirnov test and the Pearson Correlation test, respectively, performed in SPSS version 14. The p-value less than 0.05 was considered significant for the results. Results: A total of 60 samples conducted CD4 and complete blood counts (CBC) examinations during the period July-August 2017. The mean CD4 levels were 341.73 ± 243.48 cells/µL, the total leukocyte count was 6.98 ± 2.93 x 10 3 /µL, the total lymphocyte count was 2.09 ± 0.87 x 10 3 /µL, hemoglobin level 13.38 ± 2.09 g/dL, lymphocyte/ leukocyte ratio (LLR) 0.31 ± 0.11, lymphocyte/neutrophil ratio (LNR) 0.67 ± 0.40. Statistical analysis showed that data is normally distributed. Pearson correlation analysis showed that there was a significant relationship between CD4 levels with total lymphocytes counts, hemoglobin levels, RLL, RLN with correlation coefficients of 0.571 (p = 0.000), 0.324 (p = 0.012), 0.509 (p = 0.000), 0.463 (p = 0.006), respectively. There was no significant correlation between CD4 levels and total leukocytes with a correlation coefficient of 0.171 (p = 0.19). Conclusion: There is a positive correlation between CD4 levels and total lymphocytes, hemoglobin levels, RLL and RLN in patients with HIV at Sanglah Hospital.

Research paper thumbnail of Correlation of Apo E Gene Polymorphism with Recurrent Acute Coronary Syndrome

Research paper thumbnail of Implementation of Six Sigma in Glucose POCT Quality Control at Dr. Soetomo General Academic Hospital

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Point of Care Testing (POCT) is important for the examination of critically ill patients in the e... more Point of Care Testing (POCT) is important for the examination of critically ill patients in the emergency room and intensive care unit. The evaluation of the analytic quality of POCT is needed to ensure the quality of patient care at Dr. Soetomo General Academic Hospital. The purpose of this study is to evaluate the analytical quality of POCT at Dr. Soetomo General Academic Hospital. This was an observational analytical study that was done at Dr. Soetomo General Academic Hospital Surabaya in July 2017. Point of care testing quality analysis was based on 20 within run Internal Quality Control (IQC). Internal quality control data was used to calculate the mean, SD, and CV%. CV% was calculated with the following equation "CV%=(SD í— 100)/mean". Bias % = [ control mean – control true value]/control true value x 100. The sigma value was obtained from Total Error Allowance (TEa) – Bias/CV. TEa in this study was based on ISO 15197:2003. The results of the glucose examination (58 ...

Research paper thumbnail of Kinetics of anti-SARS-CoV-2 responses post complete vaccination with coronavac: A prospective study in 50 health workers

Journal of Public Health Research, Jul 1, 2022

Research paper thumbnail of SPECIFIC IGE IMMUNOBLOT METHOD IN ALLERGIC RHINITIS (IgE Spesifik Menurut Metode Imunoblot di Rinitis Alergi)

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY, 2018

Rinitis alergi merupakan penyakit bukan akibat non-infeksi yang ditemukan antara 10−30% pendudu... more Rinitis alergi merupakan penyakit bukan akibat non-infeksi yang ditemukan antara 10−30% penduduk dewasa dunia dan dapatmenyebabkan penurunan mutu kehidupan seseorang. Rinitis alergi merupakan manifestasi alergi tipe 1 atau tipe cepat yang dimediasioleh IgE. Pemeriksaan utama rinitis alergi adalah Skin Prick Test (SPT) dan IgE spesifik. Pemeriksaan IgE spesifik mempunyai kepekaandan kekhasan yang menyerupai SPT, tidak memerlukan tenaga terlatih dan menyebabkan anafilaktik. Penelitian ini untuk mengetahuiadakah kesesuaian nilai diagnostik IgE spesifik menurut metode imunoblot dengan SPT di pasien rinitis alergi dengan mengujinya.Rancangan penelitian adalah potong lintang yang dilakukan terhadap pasien yang datang di Unit Rawat Jalan THT-KL RSUDDr. Soetomo pada bulan Mei sampai dengan Oktober 2014. Pasien dikelompokkan berdasarkan diagnosis rinitis alergi dan yang nonalergidan non-infeksi serta ditetapkan secara klinis, ada riwayat alergi, pemeriksaan fisik, serta tingkat jumlah kese...

Research paper thumbnail of Potential Marker for Diagnosis and Screening of Iron Deficiency Anemia in Children

Iron Metabolism - Iron a Double‐Edged Sword [Working Title], 2022

Iron plays a role in multiple physiological functions, naming oxygen transport, gene regulation, ... more Iron plays a role in multiple physiological functions, naming oxygen transport, gene regulation, DNA synthesis, DNA repair, and brain function. Iron deficiency anemia (IDA) may happen following iron deficiency, but iron deficiency alone may cause negative impacts on the health risk of pediatric patients. The degree of iron deficiency is described by total body iron (measured by ferritin), transport iron (measured by transferrin saturation), serum iron, and other hematologic and biochemical markers. Iron deficiency anemia is a result of insufficient iron supply causing the inability to maintain normal levels of hemoglobin. The most common causes of microcytic anemia in children are iron deficiency and thalassemia minor. There are various hematologic and biochemical parameters used for screening and diagnosis of iron deficiency anemia in children, but there is no single “best” test to diagnose iron deficiency with or without anemia. The “gold standard” for identifying iron deficiency ...

Research paper thumbnail of Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods

International Journal of Nephrology

Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of ... more Background. Rapid and reliable tests are essential for the diagnostic laboratory confirmation of urinary tract infections (UTIs). Until now, UTI has been confirmed by the microbiology culture of urine, requiring at least 48-hour turnaround time (TAT), with a standardized microscopic method being widely favored. Automated urine flow cytometry, however, has recently been used to improve the rapid TAT by analyzing the urine sediment. This study therefore aimed to compare the diagnostic value of the Shih-Yung conventional microscopic and urine flow cytometry methods in the detection of leukocyte and bacterial parameters of patients with UTIs in an outpatient clinic. Methods. A cross-sectional study was conducted on a total of 100 patients. Seventy urine samples were positive for leukocytes and nitrite chemistry, and 30 were negative for both. The measurements of urine leukocytes and bacteria were compared between Sysmex UF-5000 urine flow cytometry and the Shih-Yung method. The diagnost...

Research paper thumbnail of ANALYSIS OF THE DIFFERENCE OF COMPLETENESS OF REPORTING AND DOCUMENTATION OF LABORATORY CRITICAL VALUES PRE AND POST-INTERVENTION IN BONA WARD RSUD Dr. SOETOMO SURABAYA

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY, 2019

Completeness of laboratory critical values reporting and documentation in medical records is impo... more Completeness of laboratory critical values reporting and documentation in medical records is important for patient safety, hospital accreditation, and legality. Preliminary study in Dr. Soetomo Hospital’s ward showed the laboratory critical value report and documentation was 0% complete, 4% incomplete, and 96% undocumented. This was a quasi experimental study with one group pretest and posttest design. Technical guidance of laboratory critical values reporting and documentation in medical records and supervision were given to 26 doctors. The results evaluated were doctor knowledge and attitude towards critical value reporting, completeness of documentation in medical records, and turn around time (TAT). Reporting critical values samples number was 72 before and after the intervention. The critical values samples taken by purposive sampling. The data was analyzed using Mann-Whitney test. There were significant differences in the level of knowledge, doctor’s attitudes, and completenes...

Research paper thumbnail of Relationship between Serum Dehydroepiandrosterone Levels and Heart Ejection Fraction in Heart Failure Patients

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY

Cardiovascular disease is still a serious problem in the world of health. Life expectancy after b... more Cardiovascular disease is still a serious problem in the world of health. Life expectancy after being diagnosed with heartfailure is 50% and 10% for 5 and 10 years. Steroid hormones such as Dehydroepiandrosterone (DHEAS) havecardioprotective effects by inhibiting the formation of atherosclerotic plaque, pulmonary artery vasodilators, and protectingcardiomyocytes. DHEAS levels decrease with age. Decreased DHEAS levels are associated with an increased risk ofcardiovascular disease. This study aimed to know the relationship between DHEAS levels in serum and ejection fractions inheart failure patients. This cross-sectional study used a sample of 34 people aged > 30 years who had been diagnosed withheart failure by a specialist in the Department of Cardiology and Vascular Medicine. The diagnosis of heart failure usesEchocardiography to determine the ejection heart fraction. DHEAS levels were taken from venous blood and examinedusing the CLEIA method with an IMMULITE device (Siemens He...