Rodolfo Olivera - Academia.edu (original) (raw)
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Papers by Rodolfo Olivera
Immunochemistry, 1978
Previous mvestigations which have referred to red cell Fc receptor m rabbit. guinea pig. sheep an... more Previous mvestigations which have referred to red cell Fc receptor m rabbit. guinea pig. sheep and man. have been extended to other vertebrates. such as reptiles. amphihia. birds and mammalians. In these investigations. red cells from different animal spectes have been analyzed. As ligand DNP-BSA anti-DNP non-precipitating antibody, aggregated IgG by the bis-diazotized henzidine method. IgM 7s and non-modified IgG. IgA and IgM were used. The ligand binding to red cells was detected by Coomhs test with specific anti-immunoglohulin serum. The analyzed red cells showed exposed Fc receptor. In the case of human red cells. to render such receptor evident it is necessary to submit the cells to trypsinization. All the analyzed erythrocytes hind IgM 7s. Ab-Ag complex and aggregated IgG. and some of these cells hind non-modified immunoglobulins. * Members of the Scientific Researcher Career. National Research Council of Argentina. MATERIALS AND METHODS t+~~rhroc~~~lc~.s o/' tliffiwnt nnimul ~spccics These were obtained from heparinized blood. The cells were washed 5 times with 0. I5 M NaCI. 0.01 M phosphate, pH 7.6. phosphate buffered saline (PBS) before using. These were obtained by the Morton and Pickles (1947) method. The trypsin treatment time was carried out for 10 min. Antigen-antihocij~ wntple.ws ! Ag-Ah J These were prepared by mixing 2 mg ml of antibody w'ith 250 ng,ml of antigen.
Immunochemistry, 1978
Previous mvestigations which have referred to red cell Fc receptor m rabbit. guinea pig. sheep an... more Previous mvestigations which have referred to red cell Fc receptor m rabbit. guinea pig. sheep and man. have been extended to other vertebrates. such as reptiles. amphihia. birds and mammalians. In these investigations. red cells from different animal spectes have been analyzed. As ligand DNP-BSA anti-DNP non-precipitating antibody, aggregated IgG by the bis-diazotized henzidine method. IgM 7s and non-modified IgG. IgA and IgM were used. The ligand binding to red cells was detected by Coomhs test with specific anti-immunoglohulin serum. The analyzed red cells showed exposed Fc receptor. In the case of human red cells. to render such receptor evident it is necessary to submit the cells to trypsinization. All the analyzed erythrocytes hind IgM 7s. Ab-Ag complex and aggregated IgG. and some of these cells hind non-modified immunoglobulins. * Members of the Scientific Researcher Career. National Research Council of Argentina. MATERIALS AND METHODS t+~~rhroc~~~lc~.s o/' tliffiwnt nnimul ~spccics These were obtained from heparinized blood. The cells were washed 5 times with 0. I5 M NaCI. 0.01 M phosphate, pH 7.6. phosphate buffered saline (PBS) before using. These were obtained by the Morton and Pickles (1947) method. The trypsin treatment time was carried out for 10 min. Antigen-antihocij~ wntple.ws ! Ag-Ah J These were prepared by mixing 2 mg ml of antibody w'ith 250 ng,ml of antigen.