Rogerio Lopes - Profile on Academia.edu (original) (raw)

Papers by Rogerio Lopes

Research paper thumbnail of FRANZ KAFKA — O ROMANCISTA DO "ABSURDO

FRANZ KAFKA — O ROMANCISTA DO "ABSURDO

Research paper thumbnail of JAVA APPLETS PARA A ANÁLISE DA CINEMÁTICA DO MECANISMO DE QUATRO BARRAS

JAVA APPLETS PARA A ANÁLISE DA CINEMÁTICA DO MECANISMO DE QUATRO BARRAS

Research paper thumbnail of A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams

A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams

Advances in Civil Engineering, 2011

Page 1. Hindawi Publishing Corporation Advances in Civil Engineering Volume 2011, Article ID 7253... more Page 1. Hindawi Publishing Corporation Advances in Civil Engineering Volume 2011, Article ID 725395, 9 pages doi:10.1155/2011/725395 Research Article A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams ...

Research paper thumbnail of Prediction of directional spectral emittance of packed ceramic spheres at high temperature

Prediction of directional spectral emittance of packed ceramic spheres at high temperature

High Temperatures-high Pressures, 1999

Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alum... more Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alumina, Al 2 O 3 ) is predicted by the use of a radiative model based on the discrete ordinates method associated with the control volume technique to solve the radiative transfer ...

Research paper thumbnail of A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

High Temperatures-high Pressures, 2000

Research paper thumbnail of A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

High Temperatures-High Pressures, 2000

Research paper thumbnail of Directional spectral emittance of ceramic material - Comparison of theoretical prediction and experimental data

Directional spectral emittance of ceramic material - Comparison of theoretical prediction and experimental data

7th AIAA/ASME Joint Thermophysics and Heat Transfer Conference, 1998

Research paper thumbnail of Prediction of directional spectral emittance of packed ceramic spheres at high temperature

Prediction of directional spectral emittance of packed ceramic spheres at high temperature

High Temperatures-High Pressures, 1999

Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alum... more Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alumina, Al 2 O 3 ) is predicted by the use of a radiative model based on the discrete ordinates method associated with the control volume technique to solve the radiative transfer ...

Research paper thumbnail of Criação e observações preliminares da biologia de Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) em feijão-de-porco Canavalia ensiformis (L.)

Due to the difficulty of collecting thrips in the field, laboratory rearing is essential for the ... more Due to the difficulty of collecting thrips in the field, laboratory rearing is essential for the development of basic research on biology and control. The objective of the present study was to develop a technique to rear Frankliniella occidentalis (Pergande) under laboratory conditions. Insects were multiplied on plants of Canavalia ensiformis (L.) enclosed in an acrylic container, and pollen was offered as a supplement to the adults. The ranges of temperature and humidity inside the rearing container were 25 to 280C and 80 to 95%, respectively. The development time from larva to adult was nine days, and each female produced a progeny of 14.3 larvae. The longevity of females was 8.3 days, and the viability of the larvae and pupae was 83 and 90%, respectively. Females that were kept in groups for a period of 96 hours for mating and oviposition produced 22.5 larvae each. Between 50 and 150 second-instar larvae were obtained per leaf, after seven days of incubation in the rearing system. The technique for rearing F. occidentalis developed in this study is a feasible way of obtaining large numbers of thrips for experiments on biology and on methods of control of this pest.

Research paper thumbnail of Controle do cupim subterrâneo Heterotermes tenuis (Hagen) com iscas termitrap impregnadas com inseticidas e associadas ao fungo entomopatogênico Beauveria bassiana (Bals.) Vuill

The control of the termite Heterotermes tenuis (Hagen) (Isoptera: Rhinotermitidae) was evaluated ... more The control of the termite Heterotermes tenuis (Hagen) (Isoptera: Rhinotermitidae) was evaluated using the bait/trap Termitrap impregnated with insecticides in low concentrations and associated to the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. isolate 634 (from Solenopsis invicta F. Smith) in sugarcane (Saccharum officinarum L.). The experiment was conducted in Rio Claro-SP, in a sugarcane field highly infested with termites. The treatments consisted of: imidacloprid 0,01%; imidacloprid 0,01% + B. bassiana; WG 0,003%; WG 0,003% + B. bassiana; B. bassiana; and Untreated control. Each treatment was replicated five times. The insecticides were impregnated on baits by immersion in water, being their concentrations calculated according to the weigth of the bait, and the B. bassiana was applied as pure conidia (109 conidia/bait). The research was conducted during November 1996 through April 1997. The evaluations were made after 15, 30, 41, 63, 86 e 136 days by assigning indexes to populations levels. All treatments significantly reduced termite populations when compared to the control. However, it took longer for B. bassiana alone to reduced H. tenuis population. The treatments with imidacloprid and WG were the most efficient in the control of termites in sugarcane. The baits/traps did not repel the termites.

Research paper thumbnail of Culture Media Selection for Mass Production of Isaria fumosorosea and Isaria farinosa

Brazilian Archives of Biology and Technology, 2010

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biph... more This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and whole rice and broken rice for I. fumosorosea. Results demonstrated that the two fungal species could be rapidly produced with higher yield of conidia on agro-industrial resources by using biphasic fermentation techniques.

Research paper thumbnail of Emulsifiable oils protect Metarhizium robertsii and Metarhizium pingshaense conidia from imbibitional damage

Research paper thumbnail of Biological control of insects in Brazil and China: history, current programs and reasons for their successes using entomopathogenic fungi

Biocontrol Science and Technology, 2010

Research paper thumbnail of Conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi

Journal of Invertebrate Pathology, 2015

We tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring > 16 h t... more We tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring > 16 h to germinate) are less virulent than vigorous conidia exhibiting fast germination (requiring 6 16 h to germinate). Preparations of Beauveria bassiana s.l. strain CG 1027 with variable ratios of vigorous to debilitated conidia were assayed against third-instar larvae of Spodoptera frugiperda. As the proportion of debilitated conidia in test preparations increased, LC 50 expressed in terms of total viable conidia increased, while LC 50 expressed solely in terms of vigorous conidia remained constant, indicating that vigorous conidia were responsible for nearly all mortality observed in the assays. Larvae treated with conidia from low-quality batches (with high proportions of debilitated conidia) survived consistently longer than those treated with comparable doses of conidia from high-quality batches. These results confirm our previous hypotheses that inclusion of debilitated conidia in viability assessments can lead to overestimation of the quality (potency) of mycoinsecticide preparations and support our recommendation for use of short incubation periods for assessing viability whenever viability is relied upon as an indicator of product quality.

Research paper thumbnail of Suscetibilidade da cochonilha branca dos citros, Planococcus citri, a Metarhizium anisopliae

Citrus Research & Technology, 2011

A suscetibilidade de fêmeas adultas de Planococcus citri aos fungos entomopatogênicos Metarhizium... more A suscetibilidade de fêmeas adultas de Planococcus citri aos fungos entomopatogênicos Metarhizium anisopliae, Beauveria bassiana, Isaria farinosa, I. fumosorosea, Lecanicillium muscarium e L. longisporum foi avaliada sob condições de laboratório. Os insetos foram transferidos para discos foliares de laranja doce e inoculados com as suspensões de conídios por meio de pulverização em Torre de Potter. Em seguida, os insetos foram incubados em câmara climatizada (26±1°C e 12h fotofase) por 12 dias. O fungo M. anisopliae (ESALQ-1037) foi patogênico a P. citri, causando 78% de mortalidade para fêmeas adultas e esporulação sobre todos os cadáveres. A mortalidade para os isolados das demais espécies não diferiu significativamente do controle e não foi observada colonização dos indivíduos mortos. Testes de virulência com o isolado ESALQ-1037 e com outros isolados de M. anisopliae, bem como a subsequente avaliação desse agente em condições de campo, devem ser conduzidos para futura recomendação de uso do patógeno pelo citricultor.

Research paper thumbnail of Fungo Metarhizium anisopliae e o controle de Frankliniella occidentalis em alface hidropônico

Scientia Agricola, 2000

Autor correspondente rblopes@carpa.ciagri.usp.br RESUMO: O tripes Frankliniella occidentalis (P... more Autor correspondente <rblopes@carpa.ciagri.usp.br> RESUMO: O tripes Frankliniella occidentalis (Pergande) provoca grandes prejuízos em cultivos comerciais de alface, através do dano direto, durante a alimentação no tecido vegetal, e pela transmissão de virose. O desenvolvimento de novas estratégias de controle da praga em cultivos protegidos vem se tornando necessária em função dos problemas acarretados pelo uso constante de inseticidas químicos. Foi avaliada a eficiência de pulverizações do fungo Metarhizium anisopliae (Metsch.) Sorokin (isolado 1104) nas concentrações 5x10 6 e 1x10 8 conídios ml -1 , visando o controle da praga em cultivos de alface hidropônico. A eficiência de controle do fungo nas duas concentrações chegou a 60%, seis dias após a primeira pulverização. Nas parcelas que não receberam o tratamento com o patógeno, observou-se um aumento populacional de 46,5%. Contudo, o número de adultos capturados em armadilhas adesivas foi semelhante para áreas tratadas e não tratadas, devido à alta mobilidade da praga na cultura. Constatou-se também um número crescente de adultos mortos com estruturas do patógeno, nas parcelas pulverizadas com o fungo. A ausência de toxicidade e de período de carência dos fungos entomopatogênicos torna estes microrganismos importantes na substituição de alguns inseticidas químicos na cultura do alface. Estudos mais detalhados sobre a utilização de M. anisopliae em cultivos protegidos são necessários para aplicações em grande escala. Palavras-chave: tripes, controle microbiano

Research paper thumbnail of Estabilidade de uma formulação de Bacillus sphaericus armazenada sob diferentes temperaturas

Scientia Agricola, 2001

RESUMO: O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacil... more RESUMO: O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacillus sphaericus à base de sabugo de milho, armazenada em condições ambientais e a 50°C, tendo como comparação, uma suspensão concentrada de esporos e cristais da bactéria. A análise periódica dos materiais armazenados revelaram o efeito protetor da formulação sobre o microrganismo, de forma que ao longo de 12 meses de armazenamento, em condições ambiente, a viabilidade dos esporos foi maior que na suspensão concentrada. Além disso, a atividade inseticida para larvas de Culex quinquefasciatus, avaliada nos criadouros artificiais foi comparável ao patógeno não formulado. Quando mantidos a 50°C, a ação protetora da formulação mostrou-se mais eficiente, sendo que ao final de 60 dias não houve qualquer atividade do patógeno não formulado, enquanto que na formulação obteve-se cerca de 30% de atividade, determinada em condições de laboratório. Palavras-chave: Culex quinquefasciatus, entomopatógeno, controle microbiano ABSTRACT: The stability of a Bacillus sphaericus formulation on granular corncob stored at two diferent temperatures (room temperature and 50°C) during 12 months was evaluated. A concentrated spore-cristal suspension was stored under the same conditions and used for comparison. The larvicidal activity of fresh corncobs and concentrated pathogen formulations were similar when inoculated onto artificial Culex quinquefasciatus breeding sites. Periodic analysis showed, however, higher spore viability in the corncob formulation under both conditions over a period of 12 months. The corncob formulation stored under high temperature (50°C) permitted a higher spore viability (30%) and larvicidal activity up to 60 days. Under the same conditions, the concentrated spore-cristal suspension showed total loss of both spore viability and larvicidal activity.

Research paper thumbnail of Occurence of thrips on Niagara table grape and its control with the insecticides thiacloprid and methiocarb associated with Metarhizium anisopliae

Revista Brasileira De Fruticultura, 2002

Thrips are reported as important pests on table grapes in United States and several countries of ... more Thrips are reported as important pests on table grapes in United States and several countries of Europe. Damage caused by thrips, particulary Frankliniella occidentalis, was observed on niagara table grape crop in Limeira-SP, Brazil. During the blooming period, high thrips densities were observed feeding on pollen and small berries. The symptoms left were more visible after the development of the berries and were characterized by dark scars and suberized surface on berries, sometimes causing the berry to crack, and the seed to prolapse. The effect of insecticides thiacloprid or methiocarb, associated or not with the entomopathogenic fungus Metarhizium anisopliae were evaluated during the blooming period. For evaluation of thrips damage on fruits, the treatments were applied three additional times, 7, 14 and 21 days after the first application. The treatments were: a) M. anisopliae (strain 1037) 1x10 7 conidia/mL; b) thiacloprid 20mL/100L; c-d) methiocarb 100 and 150mL/100L; e) methiocarb 100mL/100L + M. anisopliae 1x10 7 conidia/ mL. Only methiocarb, associated or not with the fungus, was effective in reducing thrips infestation, and no phytotoxic damage was observed. The efficiency of methiocarb 150mL/100L and the insecticide associated with the fungus for the control of the thrips population was 84.2 and 95.5%, respectively. In both cases, there was a reduction of approximately 70% in the number of berries with scars symptoms. For control of thrips on table grapes, chemical insecticides associated or not with M. anisopliae should be applied during the blooming period of the crop.

Research paper thumbnail of <strong>Notes on the Neotropical <em>Zethus</em> Fabricius, 1804 (Hymenoptera, Vespidae, Eumeninae) with the description of two new species from Brazil</strong>

Notes on the Neotropical Zethus Fabricius, 1804 (Hymenoptera, Vespidae, Eumeninae) with the description of two new species from Brazil

Zootaxa, 2014

Two new species, Zethus (Zethus) aliceae Lopes, sp. nov. (Brazil) and Z. (Zethus) sinuostylus Lop... more Two new species, Zethus (Zethus) aliceae Lopes, sp. nov. (Brazil) and Z. (Zethus) sinuostylus Lopes, sp. nov. (Brazil) are described and figured. New synonymy is proposed for Zethus (Zethoides) biglumis Spinola, 1841 (=Zethus ferrugineus de Saussure, 1852, syn. nov.

Research paper thumbnail of Avaliação de estirpes e de uma nova formulação granulada de Bacillus sphaericus Neide para o controle de mosquitos

Neotropical Entomology, 2006

Nineteen Bacillus sphaericus Neide strains obtained in Brazil were evaluated in addition to a sta... more Nineteen Bacillus sphaericus Neide strains obtained in Brazil were evaluated in addition to a standard strain (2362) supplied by Pasteur Institute. Most strains were more efficient than the standard, and seven of them caused mortality equal to or higher than 80%, at a low concentration (7 × 10 2 spores/ml). Strain ESALQ MS6 was selected for formulation, since it showed better yield in liquid culture medium (3 × 10 9 CFU/ml). The G4 granular formulation was tested in artificial rearing sites, consisting of plastic buckets containing 10 L water and twenty 3 rd -instar larvae of Culex quinquefasciatus Say (Diptera: Culicidae). The efficiency of formulation was compared against a commercial product, at three different concentrations. Evaluations were taken every 24h, at seven and 18 days after inoculation, with subsequent replacement of larvae in the bucket. The G4 formulation was similar to the commercial product, and controlled 100% of the larvae at the concentrations tested; however, the granules remained at the surface for a longer period. In tannery effluent treatment ponds, the G4 formulation at a concentration of 2 kg/ha reduced the number of larvae by 21%, 47%, 85%, and 94%, after 1, 3, 7, and 15 days, respectively.

Research paper thumbnail of FRANZ KAFKA — O ROMANCISTA DO "ABSURDO

FRANZ KAFKA — O ROMANCISTA DO "ABSURDO

Research paper thumbnail of JAVA APPLETS PARA A ANÁLISE DA CINEMÁTICA DO MECANISMO DE QUATRO BARRAS

JAVA APPLETS PARA A ANÁLISE DA CINEMÁTICA DO MECANISMO DE QUATRO BARRAS

Research paper thumbnail of A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams

A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams

Advances in Civil Engineering, 2011

Page 1. Hindawi Publishing Corporation Advances in Civil Engineering Volume 2011, Article ID 7253... more Page 1. Hindawi Publishing Corporation Advances in Civil Engineering Volume 2011, Article ID 725395, 9 pages doi:10.1155/2011/725395 Research Article A Matrix Formulation for the Moment Distribution Method Applied to Continuous Beams ...

Research paper thumbnail of Prediction of directional spectral emittance of packed ceramic spheres at high temperature

Prediction of directional spectral emittance of packed ceramic spheres at high temperature

High Temperatures-high Pressures, 1999

Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alum... more Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alumina, Al 2 O 3 ) is predicted by the use of a radiative model based on the discrete ordinates method associated with the control volume technique to solve the radiative transfer ...

Research paper thumbnail of A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

High Temperatures-high Pressures, 2000

Research paper thumbnail of A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

A new experimental device to measure directional spectral emittance of semitransparent media at high temperatures

High Temperatures-High Pressures, 2000

Research paper thumbnail of Directional spectral emittance of ceramic material - Comparison of theoretical prediction and experimental data

Directional spectral emittance of ceramic material - Comparison of theoretical prediction and experimental data

7th AIAA/ASME Joint Thermophysics and Heat Transfer Conference, 1998

Research paper thumbnail of Prediction of directional spectral emittance of packed ceramic spheres at high temperature

Prediction of directional spectral emittance of packed ceramic spheres at high temperature

High Temperatures-High Pressures, 1999

Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alum... more Abstract. The directional spectral emittance of absorbing and scattering isothermal ceramic (alumina, Al 2 O 3 ) is predicted by the use of a radiative model based on the discrete ordinates method associated with the control volume technique to solve the radiative transfer ...

Research paper thumbnail of Criação e observações preliminares da biologia de Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) em feijão-de-porco Canavalia ensiformis (L.)

Due to the difficulty of collecting thrips in the field, laboratory rearing is essential for the ... more Due to the difficulty of collecting thrips in the field, laboratory rearing is essential for the development of basic research on biology and control. The objective of the present study was to develop a technique to rear Frankliniella occidentalis (Pergande) under laboratory conditions. Insects were multiplied on plants of Canavalia ensiformis (L.) enclosed in an acrylic container, and pollen was offered as a supplement to the adults. The ranges of temperature and humidity inside the rearing container were 25 to 280C and 80 to 95%, respectively. The development time from larva to adult was nine days, and each female produced a progeny of 14.3 larvae. The longevity of females was 8.3 days, and the viability of the larvae and pupae was 83 and 90%, respectively. Females that were kept in groups for a period of 96 hours for mating and oviposition produced 22.5 larvae each. Between 50 and 150 second-instar larvae were obtained per leaf, after seven days of incubation in the rearing system. The technique for rearing F. occidentalis developed in this study is a feasible way of obtaining large numbers of thrips for experiments on biology and on methods of control of this pest.

Research paper thumbnail of Controle do cupim subterrâneo Heterotermes tenuis (Hagen) com iscas termitrap impregnadas com inseticidas e associadas ao fungo entomopatogênico Beauveria bassiana (Bals.) Vuill

The control of the termite Heterotermes tenuis (Hagen) (Isoptera: Rhinotermitidae) was evaluated ... more The control of the termite Heterotermes tenuis (Hagen) (Isoptera: Rhinotermitidae) was evaluated using the bait/trap Termitrap impregnated with insecticides in low concentrations and associated to the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. isolate 634 (from Solenopsis invicta F. Smith) in sugarcane (Saccharum officinarum L.). The experiment was conducted in Rio Claro-SP, in a sugarcane field highly infested with termites. The treatments consisted of: imidacloprid 0,01%; imidacloprid 0,01% + B. bassiana; WG 0,003%; WG 0,003% + B. bassiana; B. bassiana; and Untreated control. Each treatment was replicated five times. The insecticides were impregnated on baits by immersion in water, being their concentrations calculated according to the weigth of the bait, and the B. bassiana was applied as pure conidia (109 conidia/bait). The research was conducted during November 1996 through April 1997. The evaluations were made after 15, 30, 41, 63, 86 e 136 days by assigning indexes to populations levels. All treatments significantly reduced termite populations when compared to the control. However, it took longer for B. bassiana alone to reduced H. tenuis population. The treatments with imidacloprid and WG were the most efficient in the control of termites in sugarcane. The baits/traps did not repel the termites.

Research paper thumbnail of Culture Media Selection for Mass Production of Isaria fumosorosea and Isaria farinosa

Brazilian Archives of Biology and Technology, 2010

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biph... more This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and whole rice and broken rice for I. fumosorosea. Results demonstrated that the two fungal species could be rapidly produced with higher yield of conidia on agro-industrial resources by using biphasic fermentation techniques.

Research paper thumbnail of Emulsifiable oils protect Metarhizium robertsii and Metarhizium pingshaense conidia from imbibitional damage

Research paper thumbnail of Biological control of insects in Brazil and China: history, current programs and reasons for their successes using entomopathogenic fungi

Biocontrol Science and Technology, 2010

Research paper thumbnail of Conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi

Journal of Invertebrate Pathology, 2015

We tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring > 16 h t... more We tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring > 16 h to germinate) are less virulent than vigorous conidia exhibiting fast germination (requiring 6 16 h to germinate). Preparations of Beauveria bassiana s.l. strain CG 1027 with variable ratios of vigorous to debilitated conidia were assayed against third-instar larvae of Spodoptera frugiperda. As the proportion of debilitated conidia in test preparations increased, LC 50 expressed in terms of total viable conidia increased, while LC 50 expressed solely in terms of vigorous conidia remained constant, indicating that vigorous conidia were responsible for nearly all mortality observed in the assays. Larvae treated with conidia from low-quality batches (with high proportions of debilitated conidia) survived consistently longer than those treated with comparable doses of conidia from high-quality batches. These results confirm our previous hypotheses that inclusion of debilitated conidia in viability assessments can lead to overestimation of the quality (potency) of mycoinsecticide preparations and support our recommendation for use of short incubation periods for assessing viability whenever viability is relied upon as an indicator of product quality.

Research paper thumbnail of Suscetibilidade da cochonilha branca dos citros, Planococcus citri, a Metarhizium anisopliae

Citrus Research & Technology, 2011

A suscetibilidade de fêmeas adultas de Planococcus citri aos fungos entomopatogênicos Metarhizium... more A suscetibilidade de fêmeas adultas de Planococcus citri aos fungos entomopatogênicos Metarhizium anisopliae, Beauveria bassiana, Isaria farinosa, I. fumosorosea, Lecanicillium muscarium e L. longisporum foi avaliada sob condições de laboratório. Os insetos foram transferidos para discos foliares de laranja doce e inoculados com as suspensões de conídios por meio de pulverização em Torre de Potter. Em seguida, os insetos foram incubados em câmara climatizada (26±1°C e 12h fotofase) por 12 dias. O fungo M. anisopliae (ESALQ-1037) foi patogênico a P. citri, causando 78% de mortalidade para fêmeas adultas e esporulação sobre todos os cadáveres. A mortalidade para os isolados das demais espécies não diferiu significativamente do controle e não foi observada colonização dos indivíduos mortos. Testes de virulência com o isolado ESALQ-1037 e com outros isolados de M. anisopliae, bem como a subsequente avaliação desse agente em condições de campo, devem ser conduzidos para futura recomendação de uso do patógeno pelo citricultor.

Research paper thumbnail of Fungo Metarhizium anisopliae e o controle de Frankliniella occidentalis em alface hidropônico

Scientia Agricola, 2000

Autor correspondente rblopes@carpa.ciagri.usp.br RESUMO: O tripes Frankliniella occidentalis (P... more Autor correspondente <rblopes@carpa.ciagri.usp.br> RESUMO: O tripes Frankliniella occidentalis (Pergande) provoca grandes prejuízos em cultivos comerciais de alface, através do dano direto, durante a alimentação no tecido vegetal, e pela transmissão de virose. O desenvolvimento de novas estratégias de controle da praga em cultivos protegidos vem se tornando necessária em função dos problemas acarretados pelo uso constante de inseticidas químicos. Foi avaliada a eficiência de pulverizações do fungo Metarhizium anisopliae (Metsch.) Sorokin (isolado 1104) nas concentrações 5x10 6 e 1x10 8 conídios ml -1 , visando o controle da praga em cultivos de alface hidropônico. A eficiência de controle do fungo nas duas concentrações chegou a 60%, seis dias após a primeira pulverização. Nas parcelas que não receberam o tratamento com o patógeno, observou-se um aumento populacional de 46,5%. Contudo, o número de adultos capturados em armadilhas adesivas foi semelhante para áreas tratadas e não tratadas, devido à alta mobilidade da praga na cultura. Constatou-se também um número crescente de adultos mortos com estruturas do patógeno, nas parcelas pulverizadas com o fungo. A ausência de toxicidade e de período de carência dos fungos entomopatogênicos torna estes microrganismos importantes na substituição de alguns inseticidas químicos na cultura do alface. Estudos mais detalhados sobre a utilização de M. anisopliae em cultivos protegidos são necessários para aplicações em grande escala. Palavras-chave: tripes, controle microbiano

Research paper thumbnail of Estabilidade de uma formulação de Bacillus sphaericus armazenada sob diferentes temperaturas

Scientia Agricola, 2001

RESUMO: O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacil... more RESUMO: O objetivo deste trabalho foi avaliar a estabilidade de uma formulação granulada de Bacillus sphaericus à base de sabugo de milho, armazenada em condições ambientais e a 50°C, tendo como comparação, uma suspensão concentrada de esporos e cristais da bactéria. A análise periódica dos materiais armazenados revelaram o efeito protetor da formulação sobre o microrganismo, de forma que ao longo de 12 meses de armazenamento, em condições ambiente, a viabilidade dos esporos foi maior que na suspensão concentrada. Além disso, a atividade inseticida para larvas de Culex quinquefasciatus, avaliada nos criadouros artificiais foi comparável ao patógeno não formulado. Quando mantidos a 50°C, a ação protetora da formulação mostrou-se mais eficiente, sendo que ao final de 60 dias não houve qualquer atividade do patógeno não formulado, enquanto que na formulação obteve-se cerca de 30% de atividade, determinada em condições de laboratório. Palavras-chave: Culex quinquefasciatus, entomopatógeno, controle microbiano ABSTRACT: The stability of a Bacillus sphaericus formulation on granular corncob stored at two diferent temperatures (room temperature and 50°C) during 12 months was evaluated. A concentrated spore-cristal suspension was stored under the same conditions and used for comparison. The larvicidal activity of fresh corncobs and concentrated pathogen formulations were similar when inoculated onto artificial Culex quinquefasciatus breeding sites. Periodic analysis showed, however, higher spore viability in the corncob formulation under both conditions over a period of 12 months. The corncob formulation stored under high temperature (50°C) permitted a higher spore viability (30%) and larvicidal activity up to 60 days. Under the same conditions, the concentrated spore-cristal suspension showed total loss of both spore viability and larvicidal activity.

Research paper thumbnail of Occurence of thrips on Niagara table grape and its control with the insecticides thiacloprid and methiocarb associated with Metarhizium anisopliae

Revista Brasileira De Fruticultura, 2002

Thrips are reported as important pests on table grapes in United States and several countries of ... more Thrips are reported as important pests on table grapes in United States and several countries of Europe. Damage caused by thrips, particulary Frankliniella occidentalis, was observed on niagara table grape crop in Limeira-SP, Brazil. During the blooming period, high thrips densities were observed feeding on pollen and small berries. The symptoms left were more visible after the development of the berries and were characterized by dark scars and suberized surface on berries, sometimes causing the berry to crack, and the seed to prolapse. The effect of insecticides thiacloprid or methiocarb, associated or not with the entomopathogenic fungus Metarhizium anisopliae were evaluated during the blooming period. For evaluation of thrips damage on fruits, the treatments were applied three additional times, 7, 14 and 21 days after the first application. The treatments were: a) M. anisopliae (strain 1037) 1x10 7 conidia/mL; b) thiacloprid 20mL/100L; c-d) methiocarb 100 and 150mL/100L; e) methiocarb 100mL/100L + M. anisopliae 1x10 7 conidia/ mL. Only methiocarb, associated or not with the fungus, was effective in reducing thrips infestation, and no phytotoxic damage was observed. The efficiency of methiocarb 150mL/100L and the insecticide associated with the fungus for the control of the thrips population was 84.2 and 95.5%, respectively. In both cases, there was a reduction of approximately 70% in the number of berries with scars symptoms. For control of thrips on table grapes, chemical insecticides associated or not with M. anisopliae should be applied during the blooming period of the crop.

Research paper thumbnail of <strong>Notes on the Neotropical <em>Zethus</em> Fabricius, 1804 (Hymenoptera, Vespidae, Eumeninae) with the description of two new species from Brazil</strong>

Notes on the Neotropical Zethus Fabricius, 1804 (Hymenoptera, Vespidae, Eumeninae) with the description of two new species from Brazil

Zootaxa, 2014

Two new species, Zethus (Zethus) aliceae Lopes, sp. nov. (Brazil) and Z. (Zethus) sinuostylus Lop... more Two new species, Zethus (Zethus) aliceae Lopes, sp. nov. (Brazil) and Z. (Zethus) sinuostylus Lopes, sp. nov. (Brazil) are described and figured. New synonymy is proposed for Zethus (Zethoides) biglumis Spinola, 1841 (=Zethus ferrugineus de Saussure, 1852, syn. nov.

Research paper thumbnail of Avaliação de estirpes e de uma nova formulação granulada de Bacillus sphaericus Neide para o controle de mosquitos

Neotropical Entomology, 2006

Nineteen Bacillus sphaericus Neide strains obtained in Brazil were evaluated in addition to a sta... more Nineteen Bacillus sphaericus Neide strains obtained in Brazil were evaluated in addition to a standard strain (2362) supplied by Pasteur Institute. Most strains were more efficient than the standard, and seven of them caused mortality equal to or higher than 80%, at a low concentration (7 × 10 2 spores/ml). Strain ESALQ MS6 was selected for formulation, since it showed better yield in liquid culture medium (3 × 10 9 CFU/ml). The G4 granular formulation was tested in artificial rearing sites, consisting of plastic buckets containing 10 L water and twenty 3 rd -instar larvae of Culex quinquefasciatus Say (Diptera: Culicidae). The efficiency of formulation was compared against a commercial product, at three different concentrations. Evaluations were taken every 24h, at seven and 18 days after inoculation, with subsequent replacement of larvae in the bucket. The G4 formulation was similar to the commercial product, and controlled 100% of the larvae at the concentrations tested; however, the granules remained at the surface for a longer period. In tannery effluent treatment ponds, the G4 formulation at a concentration of 2 kg/ha reduced the number of larvae by 21%, 47%, 85%, and 94%, after 1, 3, 7, and 15 days, respectively.