Rogerio Tenreiro - Academia.edu (original) (raw)

Papers by Rogerio Tenreiro

Journal of Plant Pathology, 2017

Xanthomonas campestris includes pathovars X. campestris pv. campestris, X. campestris pv. raphani... more Xanthomonas campestris includes pathovars X. campestris pv. campestris, X. campestris pv. raphani and X. campestris pv. incanae, causing distinct diseases in Brassicaceae crops, ornamentals and weeds as well as on some Solanaceae crops. In Portugal, where brassica crops production has great economic importance, the presence of the first two pathovars was established. A polyphasic approach combining phenotypic, phylogenetic and genomic data was used to identify and characterize a set of 33 X. campestris isolates collected in Portugal. Pathogenicity tests on a wide host range demonstrated the presence of pathovar X. campestris pv. incanae for the first time in the country. Among X. campestris pv. campestris isolates, presence of races 4, 6 and 7 was recorded and two novel races of this pathovar, race 10 and race 11, were described. Phylogenetic relationships using gyrB gene confirmed phenotypic identification at species level and grouped X. campestris isolates in clades mostly congrue...

Plant Pathology, 2017

Xanthomonas campestris pathovars are widely distributed throughout the globe and have a broad hos... more Xanthomonas campestris pathovars are widely distributed throughout the globe and have a broad host range, causing severe economic losses in the food and ornamental crucifers markets. Using an approach based on multilocus sequence typing, phylogenetic diversity and population structure of a set of 75 Portuguese and other Xanthomonas campestris isolates from several cruciferous hosts were assessed. Although this population displayed a major clonal structure, neighbour‐net phylogenetic analysis highlighted the presence of recombinational events that may have driven the ecological specialization of X. campestris with different host ranges within the Brassicaceae family. A high level of genetic diversity within and among X. campestris pathovars was also revealed, through the establishment of 46 sequence types (STs). This approach provided a snapshot of the global X. campestris population structure in cruciferous host plants, correlating the existing pathovars with three distinct genetic ...

Plant Disease, 2015

Representing over 25% of the vegetable production, Brassicaceae crops are very important for Port... more Representing over 25% of the vegetable production, Brassicaceae crops are very important for Portuguese agriculture. Xanthomonas campestris pv. raphani (Xcr) was first described as the causative agent of a leaf spot disease affecting radish and turnip (4). Despite its ability to infect Solanaceae hosts upon inoculation, this pathogen affects mostly Brassicaceae plants. Typical symptoms include circular dark spots that become lighter and are occasionally surrounded by a chlorotic halo. In severely affected leaves, spots were not limited by the veins and coalesced into irregular shapes that perforated the leaves, rendering the plants unsuitable for marketing. In the early 2000s, several isolates causing leaf spots on Brassica oleracea varieties (cauliflower, white cabbage, savoy cabbage, and tronchuda cabbage) were collected in Sintra, near Lisbon. The isolates, identified as putative X. campestris (Xc), formed typical yellow mucoid and convex colonies when grown on YDC medium. Bioche...

Radiation inactivation of …, 2005

Page 201. Radiation Inactivation of Bioterrorism Agents 187 LG Gazso andC. C. Ponta (Eds.) IOS Pr... more Page 201. Radiation Inactivation of Bioterrorism Agents 187 LG Gazso andC. C. Ponta (Eds.) IOS Press, 2005 Detection and Prevention of Bioterrorism Agents-Portuguese Case Studies Maria Luisa BOTELHO", Sandra CABO ...

Microbiology, Dec 1, 2004

The Weissella paramesenteroides DSMZ 20288 T chromosome was analysed by pulsed-field gel electrop... more The Weissella paramesenteroides DSMZ 20288 T chromosome was analysed by pulsed-field gel electrophoresis, enabling the construction of a physical and genetic map. A total of 21 recognition sites of the restriction enzymes AscI, I-CeuI, NotI and SfiI were mapped on the chromosome, which was found to be circular with an estimated size of 2026 kb. This is believed to constitute the first study into the genomic organization of a strain of this genus, addressing the localization of important chromosomal regions such as oriC and terC. A total of 23 genetic markers were mapped, including eight rrn operons that were precisely assigned in 37 % of the W. paramesenteroides chromosome. The transcription direction of rrn loci was determined and three different rrn clusters were recognized regarding the presence/absence of tRNA genes in ITS regions.

Applied and Environmental Microbiology, Sep 1, 2008

Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utiliz... more Listeria monocytogenes is a food-borne pathogen capable of adhering to a range of surfaces utilized within the food industry, including stainless steel. The factors required for the attachment of this ubiquitous organism to abiotic surfaces are still relatively unknown. In silico analysis of the L. monocytogenes EGD genome identified a putative cell wall-anchored protein (Lmo0435 [BapL]), which had similarity to proteins involved in biofilm formation by staphylococci. An insertion mutation was constructed in L. monocytogenes to determine the influence of this protein on attachment to abiotic surfaces. The results show that the protein may contribute to the surface adherence of strains that possess BapL, but it is not an essential requirement for all L. monocytogenes strains. Several BapL-negative field isolates demonstrated an ability to adhere to abiotic surfaces equivalent to that of BapL-positive strains. BapL is not required for the virulence of L. monocytogenes in mice.

Frontiers in Microbiology

Grapes’ infection by phytopathogenic fungi may often lead to rot and impair the quality and safet... more Grapes’ infection by phytopathogenic fungi may often lead to rot and impair the quality and safety of the final product. Due to the concerns associated with the extensive use of chemicals to control these fungi, including their toxicity for environment and human health, bio-based products are being highly preferred, as eco-friendlier and safer alternatives. Specifically, yeasts have shown to possess antagonistic activity against fungi, being promising for the formulation of new biocontrol products.In this work 397 wine yeasts, isolated from Portuguese wine regions, were studied for their biocontrol potential against common grapes phytopathogenic fungal genera: Aspergillus, Botrytis, Mucor and Penicillium. This set comprised strains affiliated to 32 species distributed among 20 genera. Time-course monitoring of mold growth was performed to assess the inhibitory activity resulting from either diffusible or volatile compounds produced by each yeast strain. All yeasts displayed antagoni...

Journal of Hazardous Materials, 2022

Mycobacterium bovis causes tuberculosis (TB) at the human-wildlife-livestock interface. Environme... more Mycobacterium bovis causes tuberculosis (TB) at the human-wildlife-livestock interface. Environmental persistence of M. bovis excreted by infected hosts may cause indirect transmission to other animals. However, methodological constrains hamper assessment of M. bovis viability and molecular signature in environmental matrices. In this work, an innovative, modular, and highly efficient single-cell workflow combining flow cytometry (FLOW), fluorescence in situ hybridization (FISH), and fluorescence-activated cell sorting (FACS) was developed, allowing detection, quantification, and sorting of viable and dormant M. bovis cells from environmental matrices. Validation with spiked water and sediments showed high efficiency (90%) of cell recovery, with high linearity between expected and observed results, both in cell viability evaluation (r2 =0.93) and FISH-labelled M. bovis cells quantification (r2 ≥0.96). The limit of detection was established at 105 cells/g of soil in the cell viability step and 102 cells/g of soil in the taxonomical labelling stage. Moreover, FACS efficiency attained noteworthy recovery yield (50%) and purity (60% viable cells; 70% taxonomically labelled M. bovis). This new methodology represents a huge step for M. bovis assessment outside the mammal host, offering the rapid quantification of M. bovis cell load and cell viability, including viable but non-culturable cells, and further downstream cell analyses after FACS. Subsequent environmental data integration with the clinical component will expand knowledge on transmission routes, promising new paths in TB research and an intervention tool to mitigate the underlying biohazard.

Industrial Crops and Products, 2022

Clinical Microbiology and Infection, 2000

Scientific Reports, 2021

An amendment to this paper has been published and can be accessed via a link at the top of the pa... more An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Geoderma, 2021

Abstract Drylands are highly susceptible to degradation and climate change, which has important e... more Abstract Drylands are highly susceptible to degradation and climate change, which has important ecological and socio-economic consequences worldwide. To halt drylands degradation, plant species selection for restoration is starting to include also a functional approach, but does not integrate belowground functional traits yet. Therefore we tested the use of mycorrhizal traits to identify native plant species which host guilds of beneficial microbes and therefore enhance multiple soil functions simultaneously – soil multifunctionality. We used a soil organic matter (SOM) gradient (0.9–1.9%) and evaluated the effect of 14 common and abundant native herbaceous plant species (+bare soil) on soil functionality. We measured several soil functions (soil microbial biomass, metabolic quotient, and enzymatic activities – dehydrogenase, β-glucosidase and phosphatase) and built a soil multifunctionality index. Soil multifunctionality was strongly associated with mycorrhizal traits across the analysed SOM gradient. Bare soils and soils under non- or low-mycorrhizal plant species displayed the lower soil functionality (both individual functions and multifunctionality), while soils under Fabaceae species (Medicago truncatula, Astragalus corrugatus and Lotus halophilus) displayed the highest. For each plant species, the highest soil multifunctionality was observed at the SOM-richer site. Soil multifunctionality was strongly associated with all the mycorrhizal traits but mycorrhizal intensity and AMF spores abundance were more correlated with soil multifunctionality than mycorrhizal frequency. Our data show that: i) AM traits can be good indicators of simultaneous multiple soil functions in drylands; and ii) soil multifunctionality in drylands can be improved by management practices promoting SOM accumulation and favouring specific native plant species.

International Journal of Food Science, 2021

Yellow cured codfish has a typical yellow colour, distinctive taste, and low salt content due to ... more Yellow cured codfish has a typical yellow colour, distinctive taste, and low salt content due to its special curing process of the raw salted codfish involving several soaks in water of the raw salted codfish, alternated with drying steps. The purpose of this study was to assess the main functional groups of bacteria involved in this process and relate them with physicochemical properties of the product. A total of 28 codfish from Iceland were supplied by two local companies. Seven stages of the curing process were analyzed. From each of these seven stages, four fish samples were collected to carry out the microbial and physicochemical analyses (moisture, salt content, pH, total volatile basic nitrogen (TVB-N), and trimethylamine nitrogen (TMA-N)). Bacteria counts were performed using the MPN method and adequate culture media for aerobic, proteolytic, sulphite-reducing, biogenic amine, and trimethylamine-producing and ammonifying bacteria. Strains isolated from the highest dilutions...

Frontiers in Microbiology, 2021

Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) has been considered a strict animal pathoge... more Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) has been considered a strict animal pathogen. Nevertheless, the recent reports of human infections suggest a niche expansion for this subspecies, which may be a consequence of the virulence gene acquisition that increases its pathogenicity. Previous studies reported the presence of virulence genes of Streptococcus pyogenes phages among bovine SDSD (collected in 2002–2003); however, the identity of these mobile genetic elements remains to be clarified. Thus, this study aimed to characterize the SDSD isolates collected in 2011–2013 and compare them with SDSD isolates collected in 2002–2003 and pyogenic streptococcus genomes available at the National Center for Biotechnology Information (NCBI) database, including human SDSD and S. dysgalactiae subsp. equisimilis (SDSE) strains to track temporal shifts on bovine SDSD genotypes. The very close genetic relationships between humans SDSD and SDSE were evident from the analysis of houseke...

Scientific Reports, 2020

Animal tuberculosis (TB), caused by Mycobacterium bovis, is maintained in Portugal in a multi-hos... more Animal tuberculosis (TB), caused by Mycobacterium bovis, is maintained in Portugal in a multi-host system, with cattle, red deer and wild boar, playing a central role. However, the ecological processes driving transmission are not understood. The main aim of this study was thus to contribute to the reconstruction of the spatiotemporal history of animal TB and to refine knowledge on M. bovis population structure in order to inform novel intervention strategies. A collection of 948 M. bovis isolates obtained during long-term surveillance (2002–2016, 15 years) of cattle (n = 384), red deer (n = 303) and wild boar (n = 261), from the main TB hotspot areas, was characterized by spoligotyping and 8 to 12-loci MIRU-VNTR. Spoligotyping identified 64 profiles and MIRU-VNTR distinguished 2 to 36 subtypes within each spoligotype, enabling differentiation of mixed or clonal populations. Common genotypic profiles within and among livestock and wildlife in the same spatiotemporal context highligh...

Journal of Food and Drug Analysis, 2020

Effective epidemiological surveillance and control of Salmonella sp. requires accurate and expedi... more Effective epidemiological surveillance and control of Salmonella sp. requires accurate and expeditious genetic typing methods. In the present study, rapid PCR-based methods (ERIC-PCR, M13-PCR and RAPDs) were applied to 73 Salmonella sp. isolates, and the results compared with those previously obtained by RFLP-PFGE (Salmonella gold standard genotyping method), in order to evaluate their discriminatory ability. Results were very diverse among the primers used and, for each primer, the performance level was variable among the different serotypes. ERIC-PCR and RAPD with OPC19 was inefficient for Salmonella sp. discrimination beyond the serotype level. In opposite, M13-PCR, OPC15-RAPD and OPB17-RAPD allowed intraserotype discrimination that, in general, were less discriminative than RFLP-PFGE, indicating that should not be used as a unique typing method in epidemiological studies. Nevertheless, in particular situations, these PCR methods, which are faster and less expensive than RFLP-PFGE, could offers an attractive choice as a preliminary screening of the isolates to reduce the number of suspicious isolates that should be subsequently typed with a more discriminative and accurate methods such as RFLP-PFGE.

Frontiers in Plant Science, 2020

Hydrogen peroxide (H 2 O 2) functions as an important signaling molecule in plants during biotic ... more Hydrogen peroxide (H 2 O 2) functions as an important signaling molecule in plants during biotic interactions. However, the extent to which H 2 O 2 accumulates during these interactions and its implications in the development of disease symptoms is unclear. In this work, we provide a step-by-step optimized protocol for in situ quantification of relative H 2 O 2 concentrations in wheat leaves infected with the pathogenic bacterium Pseudomonas syringae pv. atrofaciens (Psa), either alone or in the presence of the beneficial bacterium Herbaspirillum seropedicae (RAM10). This protocol involved the use of 3-3 diaminobenzidine (DAB) staining method combined with image processing to conduct deconvolution and downstream analysis of the digitalized leaf image. The application of a linear regression model allowed to relate the intensity of the pixels resulting from DAB staining with a given concentration of H 2 O 2. Decreasing H 2 O 2 accumulation patterns were detected at increasing distances from the site of pathogen infection, and H 2 O 2 concentrations were different depending on the bacterial combinations tested. Notably, Psa-challenged plants in presence of RAM10 accumulated less H 2 O 2 in the leaf and showed reduced necrotic symptoms, pointing to a potential role of RAM10 in reducing pathogen-triggered H 2 O 2 levels in young wheat plants.

Silvae Genetica, 2008

In order to assess genetic diversity within and among populations of Pinus pinea L. (stone pine),... more In order to assess genetic diversity within and among populations of Pinus pinea L. (stone pine), seven Portuguese populations originating from three Provenance Regions were selected and genotyped using two marker systems. We compared the genetic variation of these populations using retrotransposon-based sequence-specific amplification polymorphism (SSAP) and amplified fragment length polymorphism (AFLP). In total, 105 trees were screened with three primer enzyme combinations (PEC), producing 232 SSAP and 132 AFLP loci. Where SSAP yielded approximately twice-the number of polymorphic fragments compared to AFLP. Differentiation was slightly higher for SSAP, than for AFLP (FST = 0.105 for SSAP and 0.074 for AFLP), and both significantly different from zero, P < 0.01. The levels of average genetic diversity within-population found with the two types of marker were not significantly different between SSAPs and AFLPs (26.6% and 22.8%, respectively). The populations that displayed the ...