Rohit Singh - Academia.edu (original) (raw)

Papers by Rohit Singh

International Journal of Basic & Clinical Pharmacology

Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease, exceede... more Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease, exceeded only by Alzheimer’s disease. Clinically, PD is characterized by resting tremor, rigidity (stiffness), bradykinesia (slowing), and gait dysfunction with postural instability. These are known as the classical or “cardinal” features of the disease. Levodopa remains the most effective symptomatic treatment for PD and the gold standard against which new therapies are compared. Levodopa is routinely administered in combination with a peripheral decarboxylase inhibitor to prevent its peripheral metabolism to dopamine and the development of nausea, vomiting, and orthostatic hypotension. The major concern with levodopa is that chronic levodopa treatment is associated with the development of motor complications, nausea and dizziness in the large majority of patients. We are here reporting a case of Syndopa plus (Levodopa+Carbidopa) induced dizziness in a 76 years old male patient on anti- parkin...

International Journal of Basic & Clinical Pharmacology

Gabapentin and Nortriptyline are used to treat neuropathic pain. Gabapentin belongs to a group of... more Gabapentin and Nortriptyline are used to treat neuropathic pain. Gabapentin belongs to a group of drugs called anti-convulsant and Nortriptyline belongs to tricyclic antidepressant. Gabapentin acts by binding to the specific site on voltage- gated calcium channels. This helps in relieving nerve pain and lowers the risk of seizure. Nortriptyline works by preventing the released chemical messengers in brain (serotonin and norepinephrine) from being reabsorbed back into the nerve cells. As a result, it prolongs the effect of serotonin and norepinephrine in the brain and over time, this helps to relieve depression and lighten the mood. We are reporting a case series including 3 patients having neuropathic pain and taking tablet gabapentin-nortriptyline in department of neurology, Patna medical college and hospital, Patna developed some adverse drug reactions. Causality assessment was done in each case by Naranjo scale. Each case was filled properly in ADR (adverse drug reaction) reporti...

International Journal of Basic & Clinical Pharmacology

Azathioprine is one of the triple therapy immunosuppressive agents used in patients of renal tran... more Azathioprine is one of the triple therapy immunosuppressive agents used in patients of renal transplant. Azathioprine is being used successfully in majority of patients of renal graft. But one of the dreaded complications caused by it is severe bone-marrow suppression. Here I am reporting a case of severe, life- threatening bone-marrow suppression in a 30 years old male patient of renal transplant. The case is very important and attention is needed to be given by health care professionals and by clinicians. The patient underwent renal transplant surgery in Indraprastha Apollo hospital, New Delhi. The patient was on immunosuppressive agents: prednisolone, cyclosporine and mycophenolate mofetil. After 10 months of renal transplant surgery, the patient was switched over azathioprine due to complains of GI upset due to mycophenolate mofetil. In initial 2 months there was moderate bone-marrow suppression, but afterwards there was severe bone-marrow suppression. Lastly TLC reached 300 /mm...

Construction of multi-storey building is rapidly increasing throughout the world. Advances in con... more Construction of multi-storey building is rapidly increasing throughout the world. Advances in construction technology, materials, structural systems, analysis and design software facilitated the growth of these buildings. Diagrid buildings are emerging as structurally efficient as well as architecturally significant assemblies for tall buildings. Recently the diagrid structural system has been widely used for tall buildings due to the structural efficiency and aesthetic potential provided by the unique geometric configuration of the system. Generally, for tall building diagrid structure steel is used. In present work, concrete diagrid structure is analysed and compared with conventional concrete building. Structural design of high rise buildings is governed by lateral loads due to wind or earthquake. Lateral load resistance of the structure is provided by interior structural system or exterior structural system. Due to inclined columns lateral loads are resisted by axial action of t...

Part 1 Introduction: definitions and historical background L(p)-spaces functional Banach spaces o... more Part 1 Introduction: definitions and historical background L(p)-spaces functional Banach spaces of functions locally convex function spaces. Part 2 Composition operators on L(p)-spaces: definitions, characterizations and examples invertible composition operators compact composition operators normality of composition operators weighted composition operators. Part 3 Composition operators on functional Banach spaces: general characterizations composition operators on spaces H(p)(D), H(p)(D(n)) and H(p)(D(n)) composition operators on H(p)(p(+)) composition operators on l(p)-spaces. Part 4 Composition operators on the weighted locally convex function spaces: introduction, characterization and classical results composition operators on the weighted locally convex function spaces invertible and compact composition operators on weighted function spaces. Part 5 Some applications of composition operators: isometries and composition operators ergodic theory and composition operators dynamical ...

Annals of Operations Research, 2020

Quality management has been widely discussed in the literature, and recent special issues on huma... more Quality management has been widely discussed in the literature, and recent special issues on humanitarian supply chains and relief operations have emphasized the increasing importance of quality management in this key emerging area. In this paper, we provide an extensive literature review in the field of quality management in humanitarian operations and disaster relief management. Our comprehensive review, comprising 61 articles published from 2009 to 2018, leads to the identification of enablers (e.g., transparency, policy framework), challenges (e.g., financial services, identity protection), and theory development approaches, as well as numerous research gaps that must be addressed.

Measuring Business Excellence, 2018

This paper aims to identify and examine the determinants for sustainable manufacturing in cement ... more This paper aims to identify and examine the determinants for sustainable manufacturing in cement manufacturing industry in India. Further, the study examines the causal relationship between different indicators of sustainable manufacturing.,Author (s) have identified and extracted the key dimensions of sustainable manufacturing from literature. After identification of the key dimensions, analytic hierarchy process (AHP) is used to finalize the weights and further DEMATEL is used to understand the causal relationship between key dimensions.,Findings from AHP provide the weightage to the all 13 indicators of sustainable manufacturing. From AHP, it is identified that material cost has maximum weightage, whereas material consumption has minimum weightage. From DEMATEL, it is evident that occupational health safety and safety has maximum influencing variables, whereas air emission does not have any influencing variables.,The approach adopted in the study will help firms to identify the weightage of key dimensions and further the interdependency can be developed to understand the causal relationship between different indicators of sustainable manufacturing.

The Journal of Hand Surgery (Asian-Pacific Volume), 2018

Background: Flexor tendon injuries are extremely common and they are usually the result of incise... more Background: Flexor tendon injuries are extremely common and they are usually the result of incised traumatic glass or knife injury. The process of tendon healing is a complicated and exceptionally-regimented mechanism that is originated and monitored by a vast number of diverse molecules. One of the most pivotal groups of mediators that are crucial to the healing process are growth factors (GF). Intense pulse light (IPL) can lead to evidence of new collagen formation with associated clinical improvement in tissue healing. The biological benefit of Intense pulse light (IPL) relies on judicious photothermolysis, where heat driven radiation is dissipated and focused at the cellular level. The aims of this study is to set out the effect of growth factor and IPL on healing following a tendon repair. Methods: Bovine common digital extensor tendons (CDET) were used as an ex vivo model. 44 tendon repairs were performed by the lead author using 2.5 × magnification loupes and standard instrum...

Artificial cells, nanomedicine, and biotechnology, Jan 12, 2018

RNA interference shows a great strategy for biological studies; however, delivering of small inte... more RNA interference shows a great strategy for biological studies; however, delivering of small interfering RNA (siRNA) remains challenging. Although several delivery vehicles, including cell-penetrating peptides, have been developed, their implementation is often restricted because of their endosomal entrapment. Herein, we report the formation of self-assembled nanostructures from rationally designed cyclic peptides and explore them for efficient delivery of functional biomacromolecules such as siRNA into mammalian cells. The newly obtained soft materials make stable complexes with siRNAs, thereby increasing their stability and deliver fluorescent labelled siRNA inside the cells as evident from confocal microscopy analysis. Flow cytometry analysis reveals that significant uptake of FAM-siRNA occurs in the presence of peptide nanostructures compared with siRNA alone. Peptide nanostructure-mediated delivery of very low concentration of siRNA causes significant knockdown of the target ge...

Shoulder & elbow, 2018

The sternoclavicular joint is a saddle shaped, synovial joint and is the only skeletal articulati... more The sternoclavicular joint is a saddle shaped, synovial joint and is the only skeletal articulation between the axial skeleton and the upper limb. Here, a reviewis provided of the anatomy, biomechanics, traumatic and atraumatic conditions, and management options for the various conditions described.

Journal of orthopaedics, 2018

This laboratory-based study compared distal fibula simple oblique fracture fixation with one-thir... more This laboratory-based study compared distal fibula simple oblique fracture fixation with one-third tubular plate with and without a single lag screw to determine which was mechanically more stable. A control group fixed with a limited contact dynamic compression plate was also tested. Biomechanical testing of 30 osteotomised saw bones under lateral bending and torsional forces was performed. There was no significant difference between the mean lateral bending and mean torsional stiffness between the fixation with tubular plate and lag screw and tubular plate alone. Limited contact dynamic compression plate conferred the best stability in lateral bending and torsion, as expected.

Data in brief, 2018

Data presented in this article relates to the research article entitled "Whole length myosin... more Data presented in this article relates to the research article entitled "Whole length myosin binding protein C stabilizes myosin subfragment-2 (S2) flexibility as measured by gravitational force spectroscopy." (Singh et al., 2018) [1]. The data exhibits the purified skeletal myosin binding protein C (MyBPC) from rabbit back muscle was of slow skeletal type confirmed by chromatography and in unphosphorylated state based on its isoelectric point (pI) by chromatofocussing. The competitive enzyme linked immunosorbent assay (cELISA) data displayed the site specificity of polyclonal anti-S2 antibody to myosin S2. This polyclonal antibody binding site corresponds to a familial hypertrophic cardiomyopathy (FHC) point mutation hotspot on myosin S2 illustrated in a figure of compiled data.

Archives of biochemistry and biophysics, Jan 8, 2017

The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectro... more The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectroscopy (SFS) and gravitational force spectroscopy (GFS). Experiments examined unzipping S2, since it required less force than stretching parallel to the coiled coil. Both GFS and SFS demonstrated that the force required to destabilize the light meromyosin (LMM) was greater than the force required to destabilize the coiled coil at each of three different locations along S2. GFS data also conveyed that the mechanical stability of the S2 region is independent from its association with the myosin thick filament using cofilaments of myosin tail and a single intact myosin. The C-terminal end of myosin binding protein C (MyBPC) binds to LMM and the N-terminal end can bind either S2 or actin. The force required to destabilize the myosin coiled coil molecule was 3 times greater in the presence of MyBPC than in its absence. Furthermore, the in vitro motility assay with full length slow skeletal MyB...

The Annals of The Royal College of Surgeons of England, 2016

Introduction Acute gastrointestinal stress ulceration is a common and serious complication of tra... more Introduction Acute gastrointestinal stress ulceration is a common and serious complication of trauma. Prophylactic proton pump inhibitors (PPIs) or histamine receptor antagonists have been used in poly-trauma, burns and head and spinal injuries, as well as on intensive care units, for the prevention of acute gastric stress ulcers. Methods We prospectively studied the use of prophylactic PPIs in with femoral neck fracture patients, gathering data on all acute gastric ulcer complications, including coffee-ground vomiting, malena and haematemesis. We then implemented a treatment protocol in which all patients were given prophylactic PPIs, again prospectively collecting all data. Results Five hundred and fifteen patients were included. Prior to prophylactic PPI, 15% of patients developed gastric stress ulcer complications, with 3% requiring acute intervention with oesophagogastroduodenoscopy (OGD), 5% requiring transfusions and 4% experiencing surgical delays. All patients had delayed d...

The Annals of The Royal College of Surgeons of England, 2016

BACKGROUND Subtalar dislocation occurs through disruption of two separate bony articulations: the... more BACKGROUND Subtalar dislocation occurs through disruption of two separate bony articulations: the talonavicular and talocalcaneal joints. 1 It commonly results from motor vehicle accidents, falls from a height, sporting activities, jumping, running, or twisting. 2 The majority of dislocations can be reduced in a closed manner with appropriate sedation. Failed reduction by closed means occurs in 10-20% of cases of medial and lateral dislocations. 3 A number of anatomic explanations exist for failed closed reduction, such as 'buttonholing' of the talar head through the extensor digitorum brevis, extensor retinaculum, talonavicular ligaments or joint capsule. We present a novel reduction technique using skeletal traction to assist in closed reduction.

Journal of photochemistry and photobiology. B, Biology, 2016

In this article, light induced plant extract mediated one pot synthesis of silver nanoparticles (... more In this article, light induced plant extract mediated one pot synthesis of silver nanoparticles (AgNPs) has been demonstrated and potential mechanistic insight in the synthesis has been investigated. Bioactive molecules containing medicinal plant Cassytha filiformis has been explored for the synthesis of silver nanoparticles. The as-synthesized silver nanoparticles were characterized by various analytical techniques including Ultraviolet-visible spectroscopy (UV-Vis), High Resolution Transmission Electron Microscopy (HR-TEM), Dynamic Light Scattering (DLS) and Fourier Transform Infrared Spectroscopy (FT-IR). Among different light sources (sunlight, room light, UV) applied the sunlight was found to be efficient external stimuli to induce rapid synthesis of AgNPs at room temperature. Modified DPPH assay indicated that polyphenolic compounds were most likely involved in the synthesis of AgNPs. Possible molecule responsible for the synthesis of AgNPs was identified, purified and charact...

Biochimica et biophysica acta, Jan 19, 2016

Phosphoserine aminotransferase (PSAT) catalyses the second reversible step of the phosphoserine b... more Phosphoserine aminotransferase (PSAT) catalyses the second reversible step of the phosphoserine biosynthetic pathway in Trichomonas vaginalis, which is crucial for the synthesis of serine and cysteine. PSAT from T. vaginalis (TvPSAT) was analysed using X-ray crystallography, enzyme kinetics, and molecular dynamics simulations. The crystal structure of TvPSAT was determined to 2.15Å resolution, and is the first protozoan PSAT structure to be reported. The active site of TvPSAT structure was found to be in a closed conformation, and at the active site PLP formed an internal aldimine linkage to Lys 202. In TvPSAT, Val 340 near the active site while it is Arg in most other members of the PSAT family, might be responsible in closing the active site. Kinetic studies yielded Km values of 54μM and 202μM for TvPSAT with OPLS and AKG, respectively. Only iodine inhibited the TvPSAT activity while smaller halides could not inhibit. Results from the structure, comparative molecular dynamics simu...

Journal of orthopaedic surgery (Hong Kong), 2015

To compare the outcome after early versus late surgery for closed ankle fractures in terms of the... more To compare the outcome after early versus late surgery for closed ankle fractures in terms of the length of hospital stay and infection rate. Records of 95 men and 119 women aged 14 to 92 (mean, 46) years who underwent open reduction and internal fixation for ankle fractures during three 6-month periods in 2004, 2007, and 2010 were reviewed. 82 and 132 patients underwent surgery <24 hours and >24 hours after presentation, respectively. The most common reason for delayed surgery was unavailability of the operation theatre, followed by delayed admission to the fracture clinic and excess soft tissue swelling. Patient and injury characteristics of the 3 study periods were comparable (p=0.399). The early and late surgery groups were comparable in proportions of various fracture patterns but not in patient age (40 vs. 49 years, p=0.002). The mean postoperative length of hospital stay was shorter in the early surgery group (2.9 vs. 5.5 days, p=0.009). The 2 groups did not differ sign...

Journal of Children's Orthopaedics, 2015

Purpose Although off-road motorcycling is one of the most popular sports activities practised by ... more Purpose Although off-road motorcycling is one of the most popular sports activities practised by millions of people worldwide, little has been written on motocross injuries and their prevention. In the UK alone, motocross has grown into a phenomenally ambitious and popular franchise. There are >200 motocross clubs across the country holding >900 events annually. The aim of this study is to categorise and quantify the magnitude of motocross paediatric injuries and associated morbidity. Methods Data were collected prospectively over 4 years (2010−2014) at our unit. All injuries caused by motocross biking that were referred to our trauma and orthopaedic department were included in this study, regardless of whether the rider was performing the sport competitively or recreationally. Results During the study period, 130 patients (aged 4−17 years) were identified with a total of 142 injuries, ranging from one to six injuries per patient. Most of the injuries were sustained within the...

International Journal of Basic & Clinical Pharmacology

Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease, exceede... more Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease, exceeded only by Alzheimer’s disease. Clinically, PD is characterized by resting tremor, rigidity (stiffness), bradykinesia (slowing), and gait dysfunction with postural instability. These are known as the classical or “cardinal” features of the disease. Levodopa remains the most effective symptomatic treatment for PD and the gold standard against which new therapies are compared. Levodopa is routinely administered in combination with a peripheral decarboxylase inhibitor to prevent its peripheral metabolism to dopamine and the development of nausea, vomiting, and orthostatic hypotension. The major concern with levodopa is that chronic levodopa treatment is associated with the development of motor complications, nausea and dizziness in the large majority of patients. We are here reporting a case of Syndopa plus (Levodopa+Carbidopa) induced dizziness in a 76 years old male patient on anti- parkin...

International Journal of Basic & Clinical Pharmacology

Gabapentin and Nortriptyline are used to treat neuropathic pain. Gabapentin belongs to a group of... more Gabapentin and Nortriptyline are used to treat neuropathic pain. Gabapentin belongs to a group of drugs called anti-convulsant and Nortriptyline belongs to tricyclic antidepressant. Gabapentin acts by binding to the specific site on voltage- gated calcium channels. This helps in relieving nerve pain and lowers the risk of seizure. Nortriptyline works by preventing the released chemical messengers in brain (serotonin and norepinephrine) from being reabsorbed back into the nerve cells. As a result, it prolongs the effect of serotonin and norepinephrine in the brain and over time, this helps to relieve depression and lighten the mood. We are reporting a case series including 3 patients having neuropathic pain and taking tablet gabapentin-nortriptyline in department of neurology, Patna medical college and hospital, Patna developed some adverse drug reactions. Causality assessment was done in each case by Naranjo scale. Each case was filled properly in ADR (adverse drug reaction) reporti...

International Journal of Basic & Clinical Pharmacology

Azathioprine is one of the triple therapy immunosuppressive agents used in patients of renal tran... more Azathioprine is one of the triple therapy immunosuppressive agents used in patients of renal transplant. Azathioprine is being used successfully in majority of patients of renal graft. But one of the dreaded complications caused by it is severe bone-marrow suppression. Here I am reporting a case of severe, life- threatening bone-marrow suppression in a 30 years old male patient of renal transplant. The case is very important and attention is needed to be given by health care professionals and by clinicians. The patient underwent renal transplant surgery in Indraprastha Apollo hospital, New Delhi. The patient was on immunosuppressive agents: prednisolone, cyclosporine and mycophenolate mofetil. After 10 months of renal transplant surgery, the patient was switched over azathioprine due to complains of GI upset due to mycophenolate mofetil. In initial 2 months there was moderate bone-marrow suppression, but afterwards there was severe bone-marrow suppression. Lastly TLC reached 300 /mm...

Construction of multi-storey building is rapidly increasing throughout the world. Advances in con... more Construction of multi-storey building is rapidly increasing throughout the world. Advances in construction technology, materials, structural systems, analysis and design software facilitated the growth of these buildings. Diagrid buildings are emerging as structurally efficient as well as architecturally significant assemblies for tall buildings. Recently the diagrid structural system has been widely used for tall buildings due to the structural efficiency and aesthetic potential provided by the unique geometric configuration of the system. Generally, for tall building diagrid structure steel is used. In present work, concrete diagrid structure is analysed and compared with conventional concrete building. Structural design of high rise buildings is governed by lateral loads due to wind or earthquake. Lateral load resistance of the structure is provided by interior structural system or exterior structural system. Due to inclined columns lateral loads are resisted by axial action of t...

Part 1 Introduction: definitions and historical background L(p)-spaces functional Banach spaces o... more Part 1 Introduction: definitions and historical background L(p)-spaces functional Banach spaces of functions locally convex function spaces. Part 2 Composition operators on L(p)-spaces: definitions, characterizations and examples invertible composition operators compact composition operators normality of composition operators weighted composition operators. Part 3 Composition operators on functional Banach spaces: general characterizations composition operators on spaces H(p)(D), H(p)(D(n)) and H(p)(D(n)) composition operators on H(p)(p(+)) composition operators on l(p)-spaces. Part 4 Composition operators on the weighted locally convex function spaces: introduction, characterization and classical results composition operators on the weighted locally convex function spaces invertible and compact composition operators on weighted function spaces. Part 5 Some applications of composition operators: isometries and composition operators ergodic theory and composition operators dynamical ...

Annals of Operations Research, 2020

Quality management has been widely discussed in the literature, and recent special issues on huma... more Quality management has been widely discussed in the literature, and recent special issues on humanitarian supply chains and relief operations have emphasized the increasing importance of quality management in this key emerging area. In this paper, we provide an extensive literature review in the field of quality management in humanitarian operations and disaster relief management. Our comprehensive review, comprising 61 articles published from 2009 to 2018, leads to the identification of enablers (e.g., transparency, policy framework), challenges (e.g., financial services, identity protection), and theory development approaches, as well as numerous research gaps that must be addressed.

Measuring Business Excellence, 2018

This paper aims to identify and examine the determinants for sustainable manufacturing in cement ... more This paper aims to identify and examine the determinants for sustainable manufacturing in cement manufacturing industry in India. Further, the study examines the causal relationship between different indicators of sustainable manufacturing.,Author (s) have identified and extracted the key dimensions of sustainable manufacturing from literature. After identification of the key dimensions, analytic hierarchy process (AHP) is used to finalize the weights and further DEMATEL is used to understand the causal relationship between key dimensions.,Findings from AHP provide the weightage to the all 13 indicators of sustainable manufacturing. From AHP, it is identified that material cost has maximum weightage, whereas material consumption has minimum weightage. From DEMATEL, it is evident that occupational health safety and safety has maximum influencing variables, whereas air emission does not have any influencing variables.,The approach adopted in the study will help firms to identify the weightage of key dimensions and further the interdependency can be developed to understand the causal relationship between different indicators of sustainable manufacturing.

The Journal of Hand Surgery (Asian-Pacific Volume), 2018

Background: Flexor tendon injuries are extremely common and they are usually the result of incise... more Background: Flexor tendon injuries are extremely common and they are usually the result of incised traumatic glass or knife injury. The process of tendon healing is a complicated and exceptionally-regimented mechanism that is originated and monitored by a vast number of diverse molecules. One of the most pivotal groups of mediators that are crucial to the healing process are growth factors (GF). Intense pulse light (IPL) can lead to evidence of new collagen formation with associated clinical improvement in tissue healing. The biological benefit of Intense pulse light (IPL) relies on judicious photothermolysis, where heat driven radiation is dissipated and focused at the cellular level. The aims of this study is to set out the effect of growth factor and IPL on healing following a tendon repair. Methods: Bovine common digital extensor tendons (CDET) were used as an ex vivo model. 44 tendon repairs were performed by the lead author using 2.5 × magnification loupes and standard instrum...

Artificial cells, nanomedicine, and biotechnology, Jan 12, 2018

RNA interference shows a great strategy for biological studies; however, delivering of small inte... more RNA interference shows a great strategy for biological studies; however, delivering of small interfering RNA (siRNA) remains challenging. Although several delivery vehicles, including cell-penetrating peptides, have been developed, their implementation is often restricted because of their endosomal entrapment. Herein, we report the formation of self-assembled nanostructures from rationally designed cyclic peptides and explore them for efficient delivery of functional biomacromolecules such as siRNA into mammalian cells. The newly obtained soft materials make stable complexes with siRNAs, thereby increasing their stability and deliver fluorescent labelled siRNA inside the cells as evident from confocal microscopy analysis. Flow cytometry analysis reveals that significant uptake of FAM-siRNA occurs in the presence of peptide nanostructures compared with siRNA alone. Peptide nanostructure-mediated delivery of very low concentration of siRNA causes significant knockdown of the target ge...

Shoulder & elbow, 2018

The sternoclavicular joint is a saddle shaped, synovial joint and is the only skeletal articulati... more The sternoclavicular joint is a saddle shaped, synovial joint and is the only skeletal articulation between the axial skeleton and the upper limb. Here, a reviewis provided of the anatomy, biomechanics, traumatic and atraumatic conditions, and management options for the various conditions described.

Journal of orthopaedics, 2018

This laboratory-based study compared distal fibula simple oblique fracture fixation with one-thir... more This laboratory-based study compared distal fibula simple oblique fracture fixation with one-third tubular plate with and without a single lag screw to determine which was mechanically more stable. A control group fixed with a limited contact dynamic compression plate was also tested. Biomechanical testing of 30 osteotomised saw bones under lateral bending and torsional forces was performed. There was no significant difference between the mean lateral bending and mean torsional stiffness between the fixation with tubular plate and lag screw and tubular plate alone. Limited contact dynamic compression plate conferred the best stability in lateral bending and torsion, as expected.

Data in brief, 2018

Data presented in this article relates to the research article entitled "Whole length myosin... more Data presented in this article relates to the research article entitled "Whole length myosin binding protein C stabilizes myosin subfragment-2 (S2) flexibility as measured by gravitational force spectroscopy." (Singh et al., 2018) [1]. The data exhibits the purified skeletal myosin binding protein C (MyBPC) from rabbit back muscle was of slow skeletal type confirmed by chromatography and in unphosphorylated state based on its isoelectric point (pI) by chromatofocussing. The competitive enzyme linked immunosorbent assay (cELISA) data displayed the site specificity of polyclonal anti-S2 antibody to myosin S2. This polyclonal antibody binding site corresponds to a familial hypertrophic cardiomyopathy (FHC) point mutation hotspot on myosin S2 illustrated in a figure of compiled data.

Archives of biochemistry and biophysics, Jan 8, 2017

The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectro... more The mechanical stability of the myosin subfragment-2 (S2) was tested with simulated force spectroscopy (SFS) and gravitational force spectroscopy (GFS). Experiments examined unzipping S2, since it required less force than stretching parallel to the coiled coil. Both GFS and SFS demonstrated that the force required to destabilize the light meromyosin (LMM) was greater than the force required to destabilize the coiled coil at each of three different locations along S2. GFS data also conveyed that the mechanical stability of the S2 region is independent from its association with the myosin thick filament using cofilaments of myosin tail and a single intact myosin. The C-terminal end of myosin binding protein C (MyBPC) binds to LMM and the N-terminal end can bind either S2 or actin. The force required to destabilize the myosin coiled coil molecule was 3 times greater in the presence of MyBPC than in its absence. Furthermore, the in vitro motility assay with full length slow skeletal MyB...

The Annals of The Royal College of Surgeons of England, 2016

Introduction Acute gastrointestinal stress ulceration is a common and serious complication of tra... more Introduction Acute gastrointestinal stress ulceration is a common and serious complication of trauma. Prophylactic proton pump inhibitors (PPIs) or histamine receptor antagonists have been used in poly-trauma, burns and head and spinal injuries, as well as on intensive care units, for the prevention of acute gastric stress ulcers. Methods We prospectively studied the use of prophylactic PPIs in with femoral neck fracture patients, gathering data on all acute gastric ulcer complications, including coffee-ground vomiting, malena and haematemesis. We then implemented a treatment protocol in which all patients were given prophylactic PPIs, again prospectively collecting all data. Results Five hundred and fifteen patients were included. Prior to prophylactic PPI, 15% of patients developed gastric stress ulcer complications, with 3% requiring acute intervention with oesophagogastroduodenoscopy (OGD), 5% requiring transfusions and 4% experiencing surgical delays. All patients had delayed d...

The Annals of The Royal College of Surgeons of England, 2016

BACKGROUND Subtalar dislocation occurs through disruption of two separate bony articulations: the... more BACKGROUND Subtalar dislocation occurs through disruption of two separate bony articulations: the talonavicular and talocalcaneal joints. 1 It commonly results from motor vehicle accidents, falls from a height, sporting activities, jumping, running, or twisting. 2 The majority of dislocations can be reduced in a closed manner with appropriate sedation. Failed reduction by closed means occurs in 10-20% of cases of medial and lateral dislocations. 3 A number of anatomic explanations exist for failed closed reduction, such as 'buttonholing' of the talar head through the extensor digitorum brevis, extensor retinaculum, talonavicular ligaments or joint capsule. We present a novel reduction technique using skeletal traction to assist in closed reduction.

Journal of photochemistry and photobiology. B, Biology, 2016

In this article, light induced plant extract mediated one pot synthesis of silver nanoparticles (... more In this article, light induced plant extract mediated one pot synthesis of silver nanoparticles (AgNPs) has been demonstrated and potential mechanistic insight in the synthesis has been investigated. Bioactive molecules containing medicinal plant Cassytha filiformis has been explored for the synthesis of silver nanoparticles. The as-synthesized silver nanoparticles were characterized by various analytical techniques including Ultraviolet-visible spectroscopy (UV-Vis), High Resolution Transmission Electron Microscopy (HR-TEM), Dynamic Light Scattering (DLS) and Fourier Transform Infrared Spectroscopy (FT-IR). Among different light sources (sunlight, room light, UV) applied the sunlight was found to be efficient external stimuli to induce rapid synthesis of AgNPs at room temperature. Modified DPPH assay indicated that polyphenolic compounds were most likely involved in the synthesis of AgNPs. Possible molecule responsible for the synthesis of AgNPs was identified, purified and charact...

Biochimica et biophysica acta, Jan 19, 2016

Phosphoserine aminotransferase (PSAT) catalyses the second reversible step of the phosphoserine b... more Phosphoserine aminotransferase (PSAT) catalyses the second reversible step of the phosphoserine biosynthetic pathway in Trichomonas vaginalis, which is crucial for the synthesis of serine and cysteine. PSAT from T. vaginalis (TvPSAT) was analysed using X-ray crystallography, enzyme kinetics, and molecular dynamics simulations. The crystal structure of TvPSAT was determined to 2.15Å resolution, and is the first protozoan PSAT structure to be reported. The active site of TvPSAT structure was found to be in a closed conformation, and at the active site PLP formed an internal aldimine linkage to Lys 202. In TvPSAT, Val 340 near the active site while it is Arg in most other members of the PSAT family, might be responsible in closing the active site. Kinetic studies yielded Km values of 54μM and 202μM for TvPSAT with OPLS and AKG, respectively. Only iodine inhibited the TvPSAT activity while smaller halides could not inhibit. Results from the structure, comparative molecular dynamics simu...

Journal of orthopaedic surgery (Hong Kong), 2015

To compare the outcome after early versus late surgery for closed ankle fractures in terms of the... more To compare the outcome after early versus late surgery for closed ankle fractures in terms of the length of hospital stay and infection rate. Records of 95 men and 119 women aged 14 to 92 (mean, 46) years who underwent open reduction and internal fixation for ankle fractures during three 6-month periods in 2004, 2007, and 2010 were reviewed. 82 and 132 patients underwent surgery <24 hours and >24 hours after presentation, respectively. The most common reason for delayed surgery was unavailability of the operation theatre, followed by delayed admission to the fracture clinic and excess soft tissue swelling. Patient and injury characteristics of the 3 study periods were comparable (p=0.399). The early and late surgery groups were comparable in proportions of various fracture patterns but not in patient age (40 vs. 49 years, p=0.002). The mean postoperative length of hospital stay was shorter in the early surgery group (2.9 vs. 5.5 days, p=0.009). The 2 groups did not differ sign...

Journal of Children's Orthopaedics, 2015

Purpose Although off-road motorcycling is one of the most popular sports activities practised by ... more Purpose Although off-road motorcycling is one of the most popular sports activities practised by millions of people worldwide, little has been written on motocross injuries and their prevention. In the UK alone, motocross has grown into a phenomenally ambitious and popular franchise. There are >200 motocross clubs across the country holding >900 events annually. The aim of this study is to categorise and quantify the magnitude of motocross paediatric injuries and associated morbidity. Methods Data were collected prospectively over 4 years (2010−2014) at our unit. All injuries caused by motocross biking that were referred to our trauma and orthopaedic department were included in this study, regardless of whether the rider was performing the sport competitively or recreationally. Results During the study period, 130 patients (aged 4−17 years) were identified with a total of 142 injuries, ranging from one to six injuries per patient. Most of the injuries were sustained within the...