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Papers by Rolando Barbucci

Research paper thumbnail of Macroinorganics IV: Thermodynamic functions relative to the protonation of a poly(amido-amine) with repeating unit containing 3 amino groups

Research paper thumbnail of Thermodynamics of complex formation in aqueous solution. Reactions of copper(II) with ethylenediamine, NN?-dimethylethylenediamine, and NN-dimethylethylenediamine: log K, ?H, and ?S values

Journal of the Chemical Society, Dalton Transactions, 1972

ABSTRACT

Research paper thumbnail of Preparation and ESCA characterization of poly(vinyl chloride) surf ace-grafted with heparin-complexing poly(amido amine) chains

Biomaterials, 1982

By a simple process poly(amido-amine) chains have been grafted on the surface of poly(vinyl chlor... more By a simple process poly(amido-amine) chains have been grafted on the surface of poly(vinyl chloride). Grafted poly(vinyl chloride) is able to adsorb heparin, thus providing potentially non-thrombogenic surfaces. The grafting of poly(amido-amine), and the heparin adsorption have been studied by ESCA. It has been found that the total amount of grafted poly(amido-amine) depends on the molecular weight of the poly(amido-amine) used in the grafting reaction, but the amount of heparin adsorbed on the grafted material is relatively independent of the length of the poly(amino-amine) grafted chains.

Research paper thumbnail of A new Phosphorylated Polysaccharide for Biomedical Applications: Generation of 3D Scaffolds with Osteogenic Activity and Coating of Titanium Oxide Surfaces

Macromolecular Symposia, Jun 1, 2008

... Daniela Pasqui,*1 Elisa Danieli,2 Gemma Leone,1 Paola Torricelli,3 Rolando Barbucci1,2 ... Sc... more ... Daniela Pasqui,*1 Elisa Danieli,2 Gemma Leone,1 Paola Torricelli,3 Rolando Barbucci1,2 ... Science, Surface Science Engineering Biological Response and Medical Applications, Springer-Verlag, Berlin 2001, 2. [6] S. Tosatti, R. Michel, M. Textor, ND Spencer, Langmuir 2002 18 ...

Research paper thumbnail of Preparation and physico-chemical characterisation of microporous polysaccharidic hydrogels: Selected papers from the 18th European Conference on Biomaterials (ESB2003), Stuttgart, Germany, 2003. (Guest Editors: Michael Doser and Heinrich Planck)

Journal of Materials Science Materials in Medicine, 2004

ABSTRACT In this study, a novel method of preparation of cryogels based on oxidised dextran (Ox.D... more ABSTRACT In this study, a novel method of preparation of cryogels based on oxidised dextran (Ox.D) and gelatin (Gel) without sodium borohydride is proposed. The physico-chemical properties of obtained hydrogels were investigated. It was found that the stability of cryogels Ox.D–Gel is significantly depended on pH, and mechanical properties were improved after the freeze drying. The schemes of the reactions between Ox.D and Gel at different steps of the treatment of the material were suggested. According to 1H-NMR data, the Amadori rearrangement occurred under the step of cryogelation. The increase of cross-linking degree through the formation of additional Schiff’s base group was observed as a result of incubation at high humidities or elevated temperature (60 °C). The obtained cryogels can be used as biocompatible and biodegradable scaffolds for proliferation of cells or other biomedical applications.

Research paper thumbnail of Sulphated hyaluronic acid and sulphated derivatives thereof covalently bound to polyurethanes, and the process for their preparation

Research paper thumbnail of BLOOD TUBING AND CYTOKINE PRODUCTION: EFFECT OF STERILIZATION

Renal Fail, 2001

Blood tubings commonly represent an integral component of hemodialysis circuits. Different factor... more Blood tubings commonly represent an integral component of hemodialysis circuits. Different factors may influence their biocompatibility, such as the type of material, the sterilization mode and the geometry. In vivo the final biocompatibility may be further complicated by the individual host response, the flow parameters, and the impact of mechanical trauma on blood's cellular components (i.e. erythrocytes). In this in vitro study we evaluated some commercially available blood tubings sterilized by different methods as to their interaction with normal leukocyte population and tested the response of these cells in terms of cytokines (IL-1beta, IL-1Ra, TNF-alpha). As a positive control, leukocytes were incubated with 0.5 ng/mL of bacterial lipopolysaccharide (LPS) or with Cuprophan of comparable surface. The results showed that cytokine production was markedly reduced, particularly in the case of gamma-ray-sterilized tubings. Of interest, it was not always related to the adherence. However in some cases, particularly of gamma-ray sterilization, adherence was none, despite the cytokine production.

Research paper thumbnail of Novel heparin-like sulfated polysaccharides

Research paper thumbnail of New Biliary Endoprosthesis Less Liable to Block in Biliary Infections: Description and In Vitro Studies

Eur J Surg, 1999

Objective: To test in vitro stents made from a new biomaterial that is less liable to encourage a... more Objective: To test in vitro stents made from a new biomaterial that is less liable to encourage adhesion of bacteria that may lead to blockage of the stent. Design: Laboratory experiment. Setting: University hospital, Italy. Material: 15 polyethylene biliary endoprostheses that had been removed endoscopically a mean of 151 days (range 55-416) after insertion. PUPA, a biomaterial that contains polyamidoamine cross-linked to polyurethane chains. This can bind large quantities of heparin and HyalS x (hyaluronic acid at a different stage of sulphation) in a stable fashion. Main outcome measures: Incidence of blockage and growth of pathogens in the polyethylene biliary prostheses. Adhesion of pathogens to PUPA in vitro on electron microscopy. Results: 12 of 15 polyethylene prostheses were blocked by brown concretions composed of calcium bilirubinate, palmitate, and little cholesterol. All concretions grew more than one pathogen, and the growth always included Escherichia coli. Of the 5 PUPA stents tested, only 1 had bacteria sticking to their surfaces. Conclusion: These results confirm previous studies that showed that HyalS x appreciably inhibited the adhesion of bacteria and is therefore a suitable material for the manufacture of biliary stents.

Research paper thumbnail of SULFATED HYALURONIC ACID WHEREIN THE NUMBER OF SULFATE GROUPS PER REPETITIVE UNIT IS FROM 0.5 TO LESS THAN 2

Research paper thumbnail of Thermodynamics of complex formation of aliphatic linear tetra-amines. ?H and ?S for the reactions of 1,5,9,13-tetra-azatridecane with protons and some bivalent transition-metal ions

J Chem Soc Dalton Trans, 1972

ABSTRACT

Research paper thumbnail of Thermodynamic and spectroscopic studies on the protonation of an optically active polyampholyte

Macromolecules, 1989

Page 1. 3138 Macromolecules 1989, 22, 3138-3143 Thermodynamic and Spectroscopic Studies on the Pr... more Page 1. 3138 Macromolecules 1989, 22, 3138-3143 Thermodynamic and Spectroscopic Studies on the Protonation of an Optically Active Polyampholyte Roland0 Barbucci,* Mario Casolaro, Annalisa Di Tommaso, and Agnese ...

Research paper thumbnail of Synthesis, chemical and rheological characterization of new hyaluronic acid-based hydrogels

Http Dx Doi Org 10 1163 156856200743779, Apr 2, 2012

New hyaluronic acid-based hydrogels have been synthesized. The carboxylate groups of hyaluronan w... more New hyaluronic acid-based hydrogels have been synthesized. The carboxylate groups of hyaluronan were activated in order to bind the amino terminal groups of the di-amine cross-linking reagent. Different hydrogels were obtained according to the different di-amine cross-linking agents (1,3-diaminepropane, 1,6-diaminohexane, PEG500 di-amine, and PEG800 di-amine). The crosslinked polymer (C.L.Hyal) was then sulphated (C.L.HyalS) by a heterogeneous reaction using sulphur trioxide pyridine complex (SO3-Py). The thermo-mechanical properties and swelling degree were evaluated and are discussed in relation to the chemical structure and the hydrophilic character of the gels. The different behaviours of C.L.Hyal and C.L.HyalS indicate the important role of sulphated groups.

Research paper thumbnail of Thermodynamic and 13C n.m.r. data on the protonation of polymeric bases whose repeating units behave independently towards protonation

Research paper thumbnail of Synthesis of Stimuli-Sensitive Hydrogels in the μm and sub-μm Range by Radiation Techniques and their Application

Hydrogels, 2009

ABSTRACT The macroscopic material properties (elasticity, volume in swollen state) of smart stimu... more ABSTRACT The macroscopic material properties (elasticity, volume in swollen state) of smart stimuli-sensitive hydrogels show a strong dependence on the properties of an aqueous environment (swelling agent). This behavior can be used for sensors or force-generating elements (actuators). Radiochemistry offers the possibility to synthesize smart gels in a wide range of dimensions. As an example of temperature-sensitive polymers we demonstrate the advantages of a radiochemical-based approach for hydrogel synthesis. Structures in the μm and sub-μm range on a support were synthesized using different techniques. The applications of particles and patterned layers in the μm range were demonstrated using the example of devices for handling liquids

Research paper thumbnail of Heparin immobilization on polyurethane surface; grafting of heparin complexing poly(amidoamine)s

Poly(amido-amine)s (PAA), a relatively new family of tertiary amino polymers, are discussed regar... more Poly(amido-amine)s (PAA), a relatively new family of tertiary amino polymers, are discussed regarding polymerization, molecular structure, number average molecular weight and solubilities. Their chemical properties, heparin-PAA interaction in aqueous solution, and ...

Research paper thumbnail of Preparation and properties of surface grafted materials with heparin complexing polymers

Life support systems: the journal of the European Society for Artificial Organs

Research paper thumbnail of New heparinizable materials: surface grafting of poly(amido-amine)chains on polyurethane

Research paper thumbnail of FTIR Characterization Of Poly(Amido-Amine)S Either In Solution Or Grafted On Materials

Research paper thumbnail of Predictions of the enthalpies of protonation of amines. Log K, ΔH, and ΔS values for the protonation of ethylenediamine and tri-, tetra-, penta-, and hexa-methylenediamine

Journal of the Chemical Society A Inorganic Physical Theoretical

ABSTRACT

Research paper thumbnail of Macroinorganics IV: Thermodynamic functions relative to the protonation of a poly(amido-amine) with repeating unit containing 3 amino groups

Research paper thumbnail of Thermodynamics of complex formation in aqueous solution. Reactions of copper(II) with ethylenediamine, NN?-dimethylethylenediamine, and NN-dimethylethylenediamine: log K, ?H, and ?S values

Journal of the Chemical Society, Dalton Transactions, 1972

ABSTRACT

Research paper thumbnail of Preparation and ESCA characterization of poly(vinyl chloride) surf ace-grafted with heparin-complexing poly(amido amine) chains

Biomaterials, 1982

By a simple process poly(amido-amine) chains have been grafted on the surface of poly(vinyl chlor... more By a simple process poly(amido-amine) chains have been grafted on the surface of poly(vinyl chloride). Grafted poly(vinyl chloride) is able to adsorb heparin, thus providing potentially non-thrombogenic surfaces. The grafting of poly(amido-amine), and the heparin adsorption have been studied by ESCA. It has been found that the total amount of grafted poly(amido-amine) depends on the molecular weight of the poly(amido-amine) used in the grafting reaction, but the amount of heparin adsorbed on the grafted material is relatively independent of the length of the poly(amino-amine) grafted chains.

Research paper thumbnail of A new Phosphorylated Polysaccharide for Biomedical Applications: Generation of 3D Scaffolds with Osteogenic Activity and Coating of Titanium Oxide Surfaces

Macromolecular Symposia, Jun 1, 2008

... Daniela Pasqui,*1 Elisa Danieli,2 Gemma Leone,1 Paola Torricelli,3 Rolando Barbucci1,2 ... Sc... more ... Daniela Pasqui,*1 Elisa Danieli,2 Gemma Leone,1 Paola Torricelli,3 Rolando Barbucci1,2 ... Science, Surface Science Engineering Biological Response and Medical Applications, Springer-Verlag, Berlin 2001, 2. [6] S. Tosatti, R. Michel, M. Textor, ND Spencer, Langmuir 2002 18 ...

Research paper thumbnail of Preparation and physico-chemical characterisation of microporous polysaccharidic hydrogels: Selected papers from the 18th European Conference on Biomaterials (ESB2003), Stuttgart, Germany, 2003. (Guest Editors: Michael Doser and Heinrich Planck)

Journal of Materials Science Materials in Medicine, 2004

ABSTRACT In this study, a novel method of preparation of cryogels based on oxidised dextran (Ox.D... more ABSTRACT In this study, a novel method of preparation of cryogels based on oxidised dextran (Ox.D) and gelatin (Gel) without sodium borohydride is proposed. The physico-chemical properties of obtained hydrogels were investigated. It was found that the stability of cryogels Ox.D–Gel is significantly depended on pH, and mechanical properties were improved after the freeze drying. The schemes of the reactions between Ox.D and Gel at different steps of the treatment of the material were suggested. According to 1H-NMR data, the Amadori rearrangement occurred under the step of cryogelation. The increase of cross-linking degree through the formation of additional Schiff’s base group was observed as a result of incubation at high humidities or elevated temperature (60 °C). The obtained cryogels can be used as biocompatible and biodegradable scaffolds for proliferation of cells or other biomedical applications.

Research paper thumbnail of Sulphated hyaluronic acid and sulphated derivatives thereof covalently bound to polyurethanes, and the process for their preparation

Research paper thumbnail of BLOOD TUBING AND CYTOKINE PRODUCTION: EFFECT OF STERILIZATION

Renal Fail, 2001

Blood tubings commonly represent an integral component of hemodialysis circuits. Different factor... more Blood tubings commonly represent an integral component of hemodialysis circuits. Different factors may influence their biocompatibility, such as the type of material, the sterilization mode and the geometry. In vivo the final biocompatibility may be further complicated by the individual host response, the flow parameters, and the impact of mechanical trauma on blood's cellular components (i.e. erythrocytes). In this in vitro study we evaluated some commercially available blood tubings sterilized by different methods as to their interaction with normal leukocyte population and tested the response of these cells in terms of cytokines (IL-1beta, IL-1Ra, TNF-alpha). As a positive control, leukocytes were incubated with 0.5 ng/mL of bacterial lipopolysaccharide (LPS) or with Cuprophan of comparable surface. The results showed that cytokine production was markedly reduced, particularly in the case of gamma-ray-sterilized tubings. Of interest, it was not always related to the adherence. However in some cases, particularly of gamma-ray sterilization, adherence was none, despite the cytokine production.

Research paper thumbnail of Novel heparin-like sulfated polysaccharides

Research paper thumbnail of New Biliary Endoprosthesis Less Liable to Block in Biliary Infections: Description and In Vitro Studies

Eur J Surg, 1999

Objective: To test in vitro stents made from a new biomaterial that is less liable to encourage a... more Objective: To test in vitro stents made from a new biomaterial that is less liable to encourage adhesion of bacteria that may lead to blockage of the stent. Design: Laboratory experiment. Setting: University hospital, Italy. Material: 15 polyethylene biliary endoprostheses that had been removed endoscopically a mean of 151 days (range 55-416) after insertion. PUPA, a biomaterial that contains polyamidoamine cross-linked to polyurethane chains. This can bind large quantities of heparin and HyalS x (hyaluronic acid at a different stage of sulphation) in a stable fashion. Main outcome measures: Incidence of blockage and growth of pathogens in the polyethylene biliary prostheses. Adhesion of pathogens to PUPA in vitro on electron microscopy. Results: 12 of 15 polyethylene prostheses were blocked by brown concretions composed of calcium bilirubinate, palmitate, and little cholesterol. All concretions grew more than one pathogen, and the growth always included Escherichia coli. Of the 5 PUPA stents tested, only 1 had bacteria sticking to their surfaces. Conclusion: These results confirm previous studies that showed that HyalS x appreciably inhibited the adhesion of bacteria and is therefore a suitable material for the manufacture of biliary stents.

Research paper thumbnail of SULFATED HYALURONIC ACID WHEREIN THE NUMBER OF SULFATE GROUPS PER REPETITIVE UNIT IS FROM 0.5 TO LESS THAN 2

Research paper thumbnail of Thermodynamics of complex formation of aliphatic linear tetra-amines. ?H and ?S for the reactions of 1,5,9,13-tetra-azatridecane with protons and some bivalent transition-metal ions

J Chem Soc Dalton Trans, 1972

ABSTRACT

Research paper thumbnail of Thermodynamic and spectroscopic studies on the protonation of an optically active polyampholyte

Macromolecules, 1989

Page 1. 3138 Macromolecules 1989, 22, 3138-3143 Thermodynamic and Spectroscopic Studies on the Pr... more Page 1. 3138 Macromolecules 1989, 22, 3138-3143 Thermodynamic and Spectroscopic Studies on the Protonation of an Optically Active Polyampholyte Roland0 Barbucci,* Mario Casolaro, Annalisa Di Tommaso, and Agnese ...

Research paper thumbnail of Synthesis, chemical and rheological characterization of new hyaluronic acid-based hydrogels

Http Dx Doi Org 10 1163 156856200743779, Apr 2, 2012

New hyaluronic acid-based hydrogels have been synthesized. The carboxylate groups of hyaluronan w... more New hyaluronic acid-based hydrogels have been synthesized. The carboxylate groups of hyaluronan were activated in order to bind the amino terminal groups of the di-amine cross-linking reagent. Different hydrogels were obtained according to the different di-amine cross-linking agents (1,3-diaminepropane, 1,6-diaminohexane, PEG500 di-amine, and PEG800 di-amine). The crosslinked polymer (C.L.Hyal) was then sulphated (C.L.HyalS) by a heterogeneous reaction using sulphur trioxide pyridine complex (SO3-Py). The thermo-mechanical properties and swelling degree were evaluated and are discussed in relation to the chemical structure and the hydrophilic character of the gels. The different behaviours of C.L.Hyal and C.L.HyalS indicate the important role of sulphated groups.

Research paper thumbnail of Thermodynamic and 13C n.m.r. data on the protonation of polymeric bases whose repeating units behave independently towards protonation

Research paper thumbnail of Synthesis of Stimuli-Sensitive Hydrogels in the μm and sub-μm Range by Radiation Techniques and their Application

Hydrogels, 2009

ABSTRACT The macroscopic material properties (elasticity, volume in swollen state) of smart stimu... more ABSTRACT The macroscopic material properties (elasticity, volume in swollen state) of smart stimuli-sensitive hydrogels show a strong dependence on the properties of an aqueous environment (swelling agent). This behavior can be used for sensors or force-generating elements (actuators). Radiochemistry offers the possibility to synthesize smart gels in a wide range of dimensions. As an example of temperature-sensitive polymers we demonstrate the advantages of a radiochemical-based approach for hydrogel synthesis. Structures in the μm and sub-μm range on a support were synthesized using different techniques. The applications of particles and patterned layers in the μm range were demonstrated using the example of devices for handling liquids

Research paper thumbnail of Heparin immobilization on polyurethane surface; grafting of heparin complexing poly(amidoamine)s

Poly(amido-amine)s (PAA), a relatively new family of tertiary amino polymers, are discussed regar... more Poly(amido-amine)s (PAA), a relatively new family of tertiary amino polymers, are discussed regarding polymerization, molecular structure, number average molecular weight and solubilities. Their chemical properties, heparin-PAA interaction in aqueous solution, and ...

Research paper thumbnail of Preparation and properties of surface grafted materials with heparin complexing polymers

Life support systems: the journal of the European Society for Artificial Organs

Research paper thumbnail of New heparinizable materials: surface grafting of poly(amido-amine)chains on polyurethane

Research paper thumbnail of FTIR Characterization Of Poly(Amido-Amine)S Either In Solution Or Grafted On Materials

Research paper thumbnail of Predictions of the enthalpies of protonation of amines. Log K, ΔH, and ΔS values for the protonation of ethylenediamine and tri-, tetra-, penta-, and hexa-methylenediamine

Journal of the Chemical Society A Inorganic Physical Theoretical

ABSTRACT