Roozbeh Fallahi - Academia.edu (original) (raw)

Papers by Roozbeh Fallahi

Iranian Journal of Veterinary Medicine, Sep 30, 2023

In number (16 of 22) of infants in the Dutch laboratory rabbit breeding colony, in aged 45-65 day... more In number (16 of 22) of infants in the Dutch laboratory rabbit breeding colony, in aged 45-65 days old, hair loss (alopecia) seen at the top of the head and around a week after the hair fall, was created in a state of complete alopecia. For determination of the cause of hair loss, after the observation and palpation the alopecic area, there have not been any swelling, abscesses, tumors. In skin scrapings, has not revealed the presence of any ectoparasite. Also in scratched skin samples, after lacto phenol cotton blue and specific PAS staining, there was no evidence of fungal disease. The result of bacterial and fungal culture in specific media was negative. On histopathological examination, no specific pathologic lesion was observed. For fungal dermatitis, there were no specific signs in histological sections. In autopsies of animal, certain pathological lesions were not observed in various organs and tissues. After being excluded infectious agents, full analysis of rabbit food pell...

Iranian Journal of Aquatic Animal Health, 2018

Since infectious pancreatic necrosis virus (IPNV) is the most predominant strain causing fatal vi... more Since infectious pancreatic necrosis virus (IPNV) is the most predominant strain causing fatal viral disease in Iranian fish farms, an inactivated vaccine for this fatal pathogen was developed. Additionally, the effect of the above-mentioned vaccine was investigated against IPNV using Montanide 1312 VG adjuvant. Two hundred and forty healthy fish with an average weight of 3.00 ± 0.45 g were vaccinated using immersion method with ethylene amine-inactivated IPN vaccine in combination with Montanide IMS 1312 VG for 5 min at 11 ± 1°C. The control group was vaccinated with sterile phosphate-buffered saline.

2008 IEEE International Conference on Ultra-Wideband, 2008

In this paper, a new notch frequency circular slot antenna is proposed for ultra wide-band (UWB) ... more In this paper, a new notch frequency circular slot antenna is proposed for ultra wide-band (UWB) applications. Antenna is printed on a dielectric substrate of RO4350B with relative permittivity (epsivr) of 3.4 and thickness of 500 mum. Antenna is fed by a circular open ended microstrip line is connected to a 50 Omega main line. This antenna is designed to

archives of razi institute, 2015

In this study, considering the normal ingredients in guinea pig's milk, by using cow milk, mi... more In this study, considering the normal ingredients in guinea pig's milk, by using cow milk, mixture of vitamins and minerals, a milk substitute was prepared and sterilized by autoclaving and filtration methods. In food analysis of produced milk, the important substances values, such as protein, fat and ash, were suitable. Milk substitute was fed to four groups of newborn's guinea pigs that were obtained by hysterectomy from out bred color Pirbright conventional parents, and maintained in the sterile environment inside laminar flow. The health, growth and reproductive parameters were evaluated during 6 months in four treated groups at the suckling age (milk substitute) and solid pellets feeding stage and these values were compared with four coequal's control groups. The results show, all young fed with substitute milk, reached to puberty at normal age (3-4 months old ), The males, had the normal fecundity and the duration of pregnancy in all females were in the normal rang...

Clinical and Experimental Pharmacology, Dec 29, 2017

Journal of Animal Research#R##N#(Iranian Journal of Biology), Jun 22, 2015

Bulgarian Journal of Veterinary Medicine, 2018

Several experimental studies have been conducted to determine whether environmental stressors, in... more Several experimental studies have been conducted to determine whether environmental stressors, including chemical pollutants, may have immunosuppressive effects and therefore, trigger disease emergence in exposed organisms. The present study aimed to evaluate the effect of diazinon (DZN), a worldwide used organophosphate pesticide, on some immune parameters of Caspian pond turtle (Mauremys caspica caspica). Twenty-four turtles (with a mean body weight of 182.6±10.8 g and mean age of 2 years) were randomly distributed in four groups. Three groups were intraperitoneally injected once with three different doses of DZN (1, 10, or 100 ng/g body mass), while one was maintained as control (1 mL olive oil injected). Blood samples were taken 1 and 2 weeks post-treatment. A positive correlation was found between diazinon (high dose) concentration and immunosuppressive effects at the 2 nd week post exposure as evidenced by lowered serum complement maximum haemolysis (MH%) (from 57.0% to 31.5%), diminished lysozyme activities (from 6.6 mg/L to 4.8 mg/L), increased heterophil/lymphocyte ratio (from 1.62 to 2.97), reduced leukcocyte counts (from 4.46×10 9 /L to 2.85×10 9 /L) as well as phagocytic activity (from 28.16% to 19.0%). The data demonstrated that turtles with high-dose diazinon exposure exhibited immunomodulation.

archives of razi institute, 2016

Rotaviruses group A are the major cause of diarrhea in calves under one month and every year caus... more Rotaviruses group A are the major cause of diarrhea in calves under one month and every year causes enormous economic losses. Serological and molecular techniques can be used for rapid detection of rotaviruses but virus isolation requires specific methods of cell culture and suitable cell lines. In this study, 41 samples were collected from diarrheal calves up to the age of one month, from industrial and semi-industrial farms in Tehran and Alborz provinces. The samples were positive by RT-PCR on VP6 gene. After preparation and inoculation onto MA104 cells in roller tube culture and constant cell culture system, the cytopathic effect (CPE) was observed. Eligible cultured with CPE, were confirmed by two-step RT-PCR using VP6 gene primers. Semi-nested PCR using VP7 gene primers was also performed for G-genotyping in which 11 and 2 samples were detected as G6 and G10, respectively. This is the first report on isolation and identification of rotaviruses, one of the causative agents of vi...

archives of razi institute, 2013

M ost of the systematic studies used in the development of human embryo culture media have been d... more M ost of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. The general use of NMRI outbred mice is a model for toxicology, teratology and pharmacology. NMRI mouse embryo exhibit the two-cell block in vitro. The objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygotes (NMRI) after embryo vitrification. One-cell mouse embryos were obtained from NMRI mice after superovulation and mating with adult male NMRI mice. And then randomly divided into 4 groups for culture in four different cultures media including: M16 (A), DMEM/Ham, F-12 (B), DMEM/Ham's F-12 co-culture with Vero cells(C) and DMEM/Ham's F-12 co-culture with MEF cells (D). Afterward all of the embryos were vitrified in EFS40 solution and collected. Results of our study revealed, more blastocysts significantly were developed with co-culture with MEF cells in DMEM/Ham's F-12 medium. Mor...

Summary The isolation of viral causative agent of fry trout mortality syndrome in farmed rainbow ... more Summary The isolation of viral causative agent of fry trout mortality syndrome in farmed rainbow trout in Iran was discussed. Four hundred twenty samples were collected from six provinces for virological, serological and electron microscopic examinations from October 2001 till May 2002. No cytopathic effect (CPE) was observed in the cell cultures except for EPC and BF-2 cells in which signs of CPE appeared one day postinoculation during the second and third passages of two brooder ovarian fluids. Electron microscopic examination revealed the viral particles measured 67-73×128-182nm in the pelleted materials from the CPE positive in BF-2 and EPC cell lines. Indirect immunoflurecence antibody test were positive for some of smears obtained from kidney tissues of the larvae, the samples of ovarian fluids that showed CPE on EPC and BF-2 cell lines as well as CPE positive cell cultures. Using ELISA eight of all sera samples tested were positive with optical density ranged 0.316-2.268 comp...

In rabbits, especially in some races, limb movement disorder called splay legs, are mostly unilat... more In rabbits, especially in some races, limb movement disorder called splay legs, are mostly unilateral or bilateral in front or hind legs at an early age. The severity ranges from mild to severe in this case. In breeding colony of albino Dutch laboratory rabbits, the splay leg disorder has been observed over the years permanently.Within the scope of this research, 1.5-2.5% of 700-750 number of colonies population, were affected. Splay legs were observed in 20-50 days old infants of both sexes. According to breeding system in metallic cages, it was thought that the mechanical trauma and damage to limbs has been the main cause of this problem. In this study, the accurate survey in histopathology and radiography of suffering organs was performed but any agent was seen. As some nutritional deficiencies lead to muscular atrophy and paralysis, feed analysis was also performed. In analysis of nutrients, all of the nutritional factors were in normal ranges. As is regarded, in laboratory rabb...

Theriogenology, 2018

Developmental potential of cryopreserved in vitro matured oocytes is very low in nearly all mamma... more Developmental potential of cryopreserved in vitro matured oocytes is very low in nearly all mammalian species studied to date. Despite relatively high cleavage rates, the vitrified/warmed metaphase II oocytes have a decreased rate of blastocyst formation, which can be attributed to the elevated cytoplasmic lipid content and lipid droplet fragmentation. Secretory products of ampulla oviductal epithelial cells (AECs) at the periovulatory stage of the ovarian cycle enhance the viability of in vitro matured oocytes. The present study was undertaken to determine if co-culture of cumulus-oophorus complexes (COCs) with conspecific AECs or reducing the lipid content of in vitro matured ovine oocytes would improve their cryotolerance and ensuing developmental competence. Ovine COCs aspirated from the slaughterhouse ovaries were matured in the following media or culture conditions: TCM199 þ FBS þ AECs (T1); TCM199 þ FBS (T2); TCM199 þ BSA (T3); TCM199 þ 0.6 mg/mL of L-carnitine (T4); TCM199þ Lcarnitine þ FBS (T5), or TCM199 only (Ctr). Subsequently, the oocytes were vitrified and used for in vitro fertilization (IVF). The lowest degree of zona pellucida (ZP) hardening following vitrification of in vitro matured sheep oocytes was observed in T1 and T5 (P < 0.05). Cleavage, blastocyst formation and ensuing development (i.e., total cell numbers) as well as blastocyst hatching rates were all greater (P < 0.05) in T1 compared with the remaining groups; in vitro matured COCs in T4 and Ctr did not develop beyond the cleavage stage. The inner cell mass: trophectoderm cell ratio in T1 (1:3.29) was significantly greater compared with T2 (1:3.39), T3 (1:3.40) and T5 (1:3.44). The present results indicate that the ovine COCs/ AECs co-culture system had the most positive influence on cryotolerance, ZP hardening, and developmental competence of in vitro matured oocytes.

Theriogenology, 2017

The effect of conspecific ampulla oviductal epithelial cells during in vitro maturation on oocyte... more The effect of conspecific ampulla oviductal epithelial cells during in vitro maturation on oocyte developmental competence and maturation-promoting factor (MPF) activity in sheep Short title: Oviductal epithelial cells affect ovine oocyte maturation in vitro