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Papers by Rosangela Ziech

Veterinária em Foco, 2018

Veterinaria Em Foco, Aug 21, 2014

Durante muito tempo, o bem-estar dos animais de producao foi ofuscado pela busca de melhores indi... more Durante muito tempo, o bem-estar dos animais de producao foi ofuscado pela busca de melhores indices zootecnicos. Com o passar dos anos, a sociedade passou a reconhecer a necessidade de mudancas nos sistemas de producao animal e a exigir a adocao de atitudes humanitarias na criacao e abate de animais para consumo, incluindo a bovinocultura de leite, que e um dos principais agronegocios, responsavel pela geracao de muitos empregos e renda no Brasil. Esta revisao teve como objetivo abordar os principais pontos que interferem no bem-estar de bovinos de leite, as maneiras de estima-lo e as causas de estresse relacionadas ao manejo, as enfermidadese a ambiencia.

Revista do Instituto de Laticínios Cândido Tostes

O presente trabalho objetivou avaliar o perfil tecnológico e a qualidade do leite cru refrigerado... more O presente trabalho objetivou avaliar o perfil tecnológico e a qualidade do leite cru refrigerado produzido na mesorregião oeste paranaense, região sul do Brasil, bem como a adequação do produtoaos padrões de qualidaderequeridos pela legislação brasileira. O estudo consistiu na avaliação de 50 unidades produtoras de leite;as amostras de leite cru foram coletadas a partir do tanque de refrigeração e foram realizadas análises decontagem bacteriana total (CBT), contagem de bactérias psicrotróficas (CBP) e contagem de células somáticas (CCS).Aos produtores, foi aplicado um questionário referente ao sistema e manejo de ordenha, sistema de refrigerador, conhecimento técnico dos funcionários e informações sobre o transporte do leite à indústria de processamento. A CCSestava acima do limite legal em 47,5% das amostras avaliadas, com contagem média de 9,8x105 UFC/mL; 46% das amostras estavam igualmente acima dos limites máximos previstos para CBT. O sistema de ordenha mais comumente encontra...

Brazilian Journal of Microbiology

Semina: Ciências Agrárias

Blackleg is an acute and frequently fatal infection that mainly affects cattle and is caused by C... more Blackleg is an acute and frequently fatal infection that mainly affects cattle and is caused by Clostridium chauvoei. Formalin-killed, whole-cell vaccines are commonly used to control blackleg. The aim of this study was to verify the protective efficacy of two commercial vaccines against the infection of guinea pigs with two strains of C. chauvoei, a virulent field strain (SBP 07/09) and the reference strain used in official tests (Manguinhos-Teixeira or MT). The strains used in the challenge were characterized by whole genome sequencing, and the minimal inhibitory concentrations of 15 antimicrobials were determined. To assess the protective efficacy, guinea pigs were vaccinated and subsequently challenged with C. chauvoei. All four vaccinated and challenged groups seroconverted after vaccination, while the control group remained seronegative, as determined by indirect ELISA. The identical performance of the two C. chauvoei strains in terms of virulence after challenge and their ina...

Ciência Rural

ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiolo... more ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiologic agent is the anaerobic bacterium Clostridium chauvoei. In recent years, the major virulence factors of C. chauvoei have been discovered and described. However, the pathogenesis of blackleg in cattle, and in particular, the movement of the pathogen from the point of entry to the affected tissues is not yet fully elucidated. Disease control is based on appropriate management and vaccination. This review summarizes the latest research findings that contribute toward the understanding of the disease in cattle, provide a foundation to preventive strategies, and identify future research needs.

Frontiers in Microbiology

Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of... more Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of cattle and sheep, isolated from four different continents over a period of 64 years (1951-2015) were determined and analyzed. The study reveals that the genome of the species C. chauvoei is highly homogeneous compared to the closely related species C. perfringens, a widespread pathogen that affects human and many animal species. Analysis of the CRISPR locus is sufficient to differentiate most C. chauvoei strains and is the most heterogenous region in the genome, containing in total 187 different spacer elements that are distributed as 30-77 copies in the various strains. Some genetic differences are found in the 3 allelic variants of fliC1, fliC2 and fliC3 genes that encode structural flagellin proteins, and certain strains do only contain one or two alleles. However, the major virulence genes including the highly toxic C. chauvoei toxin A, the sialidase and the two hyaluronidases are fully conserved as are the metabolic and structural genes of C. chauvoei. These data indicate that C. chauvoei is a strict ruminant-associated pathogen that has reached a dead end in its evolution.

Ciência Rural

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter f... more ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus subsp. venerealis. In countries with large cattle herds, such as Brazil, where the use of natural breeding as a reproductive strategy is a common practice, BGC is considered an important cause of reproductive failure and economic losses. In these cases, the bull is the asymptomatic carrier of the bacterium and the infected females can have infertility and even abortions. The techniques for the diagnosis of C. fetus are isolation in culture medium and identification by biochemical tests, immunofluorescence, immunoenzymatic assays and molecular techniques. Disease control is based on vaccination with bacterins. This review described the epidemiology, etiology, pathogenesis, and advances in the diagnosis and control of BGC.

Brazilian Journal of Microbiology, 2016

The aim of this study was to investigate the occurrence of multidrug-resistant, extended spectrum... more The aim of this study was to investigate the occurrence of multidrug-resistant, extended spectrum beta-lactamase (ESBL) producing Salmonella spp. isolated from conveyor belts of broiler cutting rooms in Brazilian broiler processing plants. Ninety-eight strains of Salmonella spp. were analyzed. Multidrug resistance was determined by the disk diffusion test and the susceptibility of the isolated bacteria was evaluated against 18 antimicrobials from seven different classes. The double disk diffusion test was used to evaluate ESBL production. Of the 98 strains tested, 84 were multidrug resistant. The highest rates of resistance were against nalidixic acid (95%), tetracycline (91%), and the beta-lactams: ampicillin and cefachlor (45%), followed by streptomycin and gentamicin with 19% and 15% of strain resistance, respectively. By contrast, 97% of the strains were sensitive to chloramphenicol. 45% of the strains were positive for the presence of ESBL activity. In this study, high rates of multidrug resistance and ESBL production were observed in Salmonella spp.

LWT - Food Science and Technology, 2016

Ciência Rural

Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, alé... more Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, além de aumentar o intervalo entre partos. A ocorrência de CGB entre rebanhos no Brasil tem variado muito entre as diferentes regiões. Com isso, o objetivo deste trabalho foi identifi car, por meio da reação em cadeia da polimerase (PCR), a ocorrência de amostras positivas para C. fetus, oriundas de bovinos, no período de 1999 a 2010, no Rio Grande do Sul, e analisar a positividade em machos e fêmeas. Foram utilizadas 816 amostras procedentes de 37 municípios, localizados predominantemente nas mesorregiões sudoeste e centro ocidental rio-grandense, das quais 480 aspirados prepuciais (92 provenientes de duas centrais de inseminação artifi cial e 388 de estabelecimentos de criação -monta natural), 324 aspirados cervicais e conteúdo abomasal de 12 fetos bovinos abortados. Como resultado, 10,9% das amostras (89/816) foram positivas para C. fetus. Quando analisados os resultados em relação à origem das amostras, 6,5% (6/92) das coletadas de machos de centrais de inseminação foram positivas, e das obtidas de touros utilizados em monta natural, 9% (35/388). Já entre as fêmeas, esse percentual foi de 13,6% (44/324) e, nas amostras obtidas de fetos abortados, 33,3% (4/12) foram positivas. Quando analisados os 91 estabelecimentos de criação com monta natural e os 37 municípios, foram positivos 44,0% (40/91) e 63,2% (24/37), respectivamente. Com isso, foi demonstrada a importância da CGB para os rebanhos bovinos, e uma maior ocorrência de amostras positivas em fêmeas, quando comparadas às amostras provenientes de machos. Palavras-chave: doença venérea, campilobacteriose genital bovina, diagnóstico, frequência. ABSTRACT Bovine genital campylobacteriosis (BGC) is an infectious disease caused by Campylobacter fetus, which

Pesquisa Veterinária Brasileira

The aim of this study was to investigate the antimicrobial activity of the oleoresin Copaifera re... more The aim of this study was to investigate the antimicrobial activity of the oleoresin Copaifera reticulata Ducke against Staphylococcus coagulase positive (SCP) isolated from otitis externa in dogs. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oleoresin were determined by broth microdilution method. In addition, we verified the antimicrobial susceptibility profile of the isolates of SCP by agar diffusion method. Eight classes of antimicrobial were used to calculate the multidrug resistance. The chemical composition of the oleoresin was performed by gas chromatography coupled to the mass spectrometry (GC/MS), and β-caryophyllene, β-bisabolene, and (E)-α-bergamotene were the main compounds found. The copaiba oleoresin showed a MIC90 of 0.164mg/mL and a CBM90 of 1.3mg/mL. The multidrug resistance was found in 27% of the strains tested. The results suggest that copaiba oleoresin has bacteriostatic and bactericidal activity even in multidr...

Anais da Academia Brasileira de Ciências, 2015

In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was eva... more In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL -1 for L. alba EO and 0.5 and 1.2 µg mL -1 for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L -1 of gentamicin, and 20 or 50 µL L -1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L -1 ) was greater than 90%.

Ciência Rural, 2014

ABSTRACT RESUMO A campilobacteriose genital bovina (CGB) é uma doença infectocontagiosa causada p... more ABSTRACT RESUMO A campilobacteriose genital bovina (CGB) é uma doença infectocontagiosa causada por Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, além de aumentar o intervalo entre partos. A ocorrência de CGB entre rebanhos no Brasil tem variado muito entre as diferentes regiões. Com isso, o objetivo deste trabalho foi identifi car, por meio da reação em cadeia da polimerase (PCR), a ocorrência de amostras positivas para C. fetus, oriundas de bovinos, no período de 1999 a 2010, no Rio Grande do Sul, e analisar a positividade em machos e fêmeas. Foram utilizadas 816 amostras procedentes de 37 municípios, localizados predominantemente nas mesorregiões sudoeste e centro ocidental rio-grandense, das quais 480 aspirados prepuciais (92 provenientes de duas centrais de inseminação artifi cial e 388 de estabelecimentos de criação -monta natural), 324 aspirados cervicais e conteúdo abomasal de 12 fetos bovinos abortados. Como resultado, 10,9% das amostras (89/816) foram positivas para C. fetus. Quando analisados os resultados em relação à origem das amostras, 6,5% (6/92) das coletadas de machos de centrais de inseminação foram positivas, e das obtidas de touros utilizados em monta natural, 9% (35/388). Já entre as fêmeas, esse percentual foi de 13,6% (44/324) e, nas amostras obtidas de fetos abortados, 33,3% (4/12) foram positivas. Quando analisados os 91 estabelecimentos de criação com monta natural e os 37 municípios, foram positivos 44,0% (40/91) e 63,2% (24/37), respectivamente. Palavras-chave: doença venérea, campilobacteriose genital bovina, diagnóstico, frequência. ABSTRACT Bovine genital campylobacteriosis (BGC) is an infectious disease caused by Campylobacter fetus, which

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Pesquisa Veterinária Brasileira, 2013

Veterinária em Foco, 2018

Veterinaria Em Foco, Aug 21, 2014

Durante muito tempo, o bem-estar dos animais de producao foi ofuscado pela busca de melhores indi... more Durante muito tempo, o bem-estar dos animais de producao foi ofuscado pela busca de melhores indices zootecnicos. Com o passar dos anos, a sociedade passou a reconhecer a necessidade de mudancas nos sistemas de producao animal e a exigir a adocao de atitudes humanitarias na criacao e abate de animais para consumo, incluindo a bovinocultura de leite, que e um dos principais agronegocios, responsavel pela geracao de muitos empregos e renda no Brasil. Esta revisao teve como objetivo abordar os principais pontos que interferem no bem-estar de bovinos de leite, as maneiras de estima-lo e as causas de estresse relacionadas ao manejo, as enfermidadese a ambiencia.

Revista do Instituto de Laticínios Cândido Tostes

O presente trabalho objetivou avaliar o perfil tecnológico e a qualidade do leite cru refrigerado... more O presente trabalho objetivou avaliar o perfil tecnológico e a qualidade do leite cru refrigerado produzido na mesorregião oeste paranaense, região sul do Brasil, bem como a adequação do produtoaos padrões de qualidaderequeridos pela legislação brasileira. O estudo consistiu na avaliação de 50 unidades produtoras de leite;as amostras de leite cru foram coletadas a partir do tanque de refrigeração e foram realizadas análises decontagem bacteriana total (CBT), contagem de bactérias psicrotróficas (CBP) e contagem de células somáticas (CCS).Aos produtores, foi aplicado um questionário referente ao sistema e manejo de ordenha, sistema de refrigerador, conhecimento técnico dos funcionários e informações sobre o transporte do leite à indústria de processamento. A CCSestava acima do limite legal em 47,5% das amostras avaliadas, com contagem média de 9,8x105 UFC/mL; 46% das amostras estavam igualmente acima dos limites máximos previstos para CBT. O sistema de ordenha mais comumente encontra...

Brazilian Journal of Microbiology

Semina: Ciências Agrárias

Blackleg is an acute and frequently fatal infection that mainly affects cattle and is caused by C... more Blackleg is an acute and frequently fatal infection that mainly affects cattle and is caused by Clostridium chauvoei. Formalin-killed, whole-cell vaccines are commonly used to control blackleg. The aim of this study was to verify the protective efficacy of two commercial vaccines against the infection of guinea pigs with two strains of C. chauvoei, a virulent field strain (SBP 07/09) and the reference strain used in official tests (Manguinhos-Teixeira or MT). The strains used in the challenge were characterized by whole genome sequencing, and the minimal inhibitory concentrations of 15 antimicrobials were determined. To assess the protective efficacy, guinea pigs were vaccinated and subsequently challenged with C. chauvoei. All four vaccinated and challenged groups seroconverted after vaccination, while the control group remained seronegative, as determined by indirect ELISA. The identical performance of the two C. chauvoei strains in terms of virulence after challenge and their ina...

Ciência Rural

ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiolo... more ABSTRACT: Blackleg is an endogenous acute infection that principally affects cattle, whose etiologic agent is the anaerobic bacterium Clostridium chauvoei. In recent years, the major virulence factors of C. chauvoei have been discovered and described. However, the pathogenesis of blackleg in cattle, and in particular, the movement of the pathogen from the point of entry to the affected tissues is not yet fully elucidated. Disease control is based on appropriate management and vaccination. This review summarizes the latest research findings that contribute toward the understanding of the disease in cattle, provide a foundation to preventive strategies, and identify future research needs.

Frontiers in Microbiology

Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of... more Full genome sequences of 20 strains of Clostridium chauvoei, the etiological agent of blackleg of cattle and sheep, isolated from four different continents over a period of 64 years (1951-2015) were determined and analyzed. The study reveals that the genome of the species C. chauvoei is highly homogeneous compared to the closely related species C. perfringens, a widespread pathogen that affects human and many animal species. Analysis of the CRISPR locus is sufficient to differentiate most C. chauvoei strains and is the most heterogenous region in the genome, containing in total 187 different spacer elements that are distributed as 30-77 copies in the various strains. Some genetic differences are found in the 3 allelic variants of fliC1, fliC2 and fliC3 genes that encode structural flagellin proteins, and certain strains do only contain one or two alleles. However, the major virulence genes including the highly toxic C. chauvoei toxin A, the sialidase and the two hyaluronidases are fully conserved as are the metabolic and structural genes of C. chauvoei. These data indicate that C. chauvoei is a strict ruminant-associated pathogen that has reached a dead end in its evolution.

Ciência Rural

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter f... more ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal disease caused by Campylobacter fetus subsp. venerealis. In countries with large cattle herds, such as Brazil, where the use of natural breeding as a reproductive strategy is a common practice, BGC is considered an important cause of reproductive failure and economic losses. In these cases, the bull is the asymptomatic carrier of the bacterium and the infected females can have infertility and even abortions. The techniques for the diagnosis of C. fetus are isolation in culture medium and identification by biochemical tests, immunofluorescence, immunoenzymatic assays and molecular techniques. Disease control is based on vaccination with bacterins. This review described the epidemiology, etiology, pathogenesis, and advances in the diagnosis and control of BGC.

Brazilian Journal of Microbiology, 2016

The aim of this study was to investigate the occurrence of multidrug-resistant, extended spectrum... more The aim of this study was to investigate the occurrence of multidrug-resistant, extended spectrum beta-lactamase (ESBL) producing Salmonella spp. isolated from conveyor belts of broiler cutting rooms in Brazilian broiler processing plants. Ninety-eight strains of Salmonella spp. were analyzed. Multidrug resistance was determined by the disk diffusion test and the susceptibility of the isolated bacteria was evaluated against 18 antimicrobials from seven different classes. The double disk diffusion test was used to evaluate ESBL production. Of the 98 strains tested, 84 were multidrug resistant. The highest rates of resistance were against nalidixic acid (95%), tetracycline (91%), and the beta-lactams: ampicillin and cefachlor (45%), followed by streptomycin and gentamicin with 19% and 15% of strain resistance, respectively. By contrast, 97% of the strains were sensitive to chloramphenicol. 45% of the strains were positive for the presence of ESBL activity. In this study, high rates of multidrug resistance and ESBL production were observed in Salmonella spp.

LWT - Food Science and Technology, 2016

Ciência Rural

Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, alé... more Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, além de aumentar o intervalo entre partos. A ocorrência de CGB entre rebanhos no Brasil tem variado muito entre as diferentes regiões. Com isso, o objetivo deste trabalho foi identifi car, por meio da reação em cadeia da polimerase (PCR), a ocorrência de amostras positivas para C. fetus, oriundas de bovinos, no período de 1999 a 2010, no Rio Grande do Sul, e analisar a positividade em machos e fêmeas. Foram utilizadas 816 amostras procedentes de 37 municípios, localizados predominantemente nas mesorregiões sudoeste e centro ocidental rio-grandense, das quais 480 aspirados prepuciais (92 provenientes de duas centrais de inseminação artifi cial e 388 de estabelecimentos de criação -monta natural), 324 aspirados cervicais e conteúdo abomasal de 12 fetos bovinos abortados. Como resultado, 10,9% das amostras (89/816) foram positivas para C. fetus. Quando analisados os resultados em relação à origem das amostras, 6,5% (6/92) das coletadas de machos de centrais de inseminação foram positivas, e das obtidas de touros utilizados em monta natural, 9% (35/388). Já entre as fêmeas, esse percentual foi de 13,6% (44/324) e, nas amostras obtidas de fetos abortados, 33,3% (4/12) foram positivas. Quando analisados os 91 estabelecimentos de criação com monta natural e os 37 municípios, foram positivos 44,0% (40/91) e 63,2% (24/37), respectivamente. Com isso, foi demonstrada a importância da CGB para os rebanhos bovinos, e uma maior ocorrência de amostras positivas em fêmeas, quando comparadas às amostras provenientes de machos. Palavras-chave: doença venérea, campilobacteriose genital bovina, diagnóstico, frequência. ABSTRACT Bovine genital campylobacteriosis (BGC) is an infectious disease caused by Campylobacter fetus, which

Pesquisa Veterinária Brasileira

The aim of this study was to investigate the antimicrobial activity of the oleoresin Copaifera re... more The aim of this study was to investigate the antimicrobial activity of the oleoresin Copaifera reticulata Ducke against Staphylococcus coagulase positive (SCP) isolated from otitis externa in dogs. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the oleoresin were determined by broth microdilution method. In addition, we verified the antimicrobial susceptibility profile of the isolates of SCP by agar diffusion method. Eight classes of antimicrobial were used to calculate the multidrug resistance. The chemical composition of the oleoresin was performed by gas chromatography coupled to the mass spectrometry (GC/MS), and β-caryophyllene, β-bisabolene, and (E)-α-bergamotene were the main compounds found. The copaiba oleoresin showed a MIC90 of 0.164mg/mL and a CBM90 of 1.3mg/mL. The multidrug resistance was found in 27% of the strains tested. The results suggest that copaiba oleoresin has bacteriostatic and bactericidal activity even in multidr...

Anais da Academia Brasileira de Ciências, 2015

In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was eva... more In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL -1 for L. alba EO and 0.5 and 1.2 µg mL -1 for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L -1 of gentamicin, and 20 or 50 µL L -1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L -1 ) was greater than 90%.

Ciência Rural, 2014

ABSTRACT RESUMO A campilobacteriose genital bovina (CGB) é uma doença infectocontagiosa causada p... more ABSTRACT RESUMO A campilobacteriose genital bovina (CGB) é uma doença infectocontagiosa causada por Campylobacter fetus, determina infertilidade temporária, endometrite leve e aborto em fêmeas, além de aumentar o intervalo entre partos. A ocorrência de CGB entre rebanhos no Brasil tem variado muito entre as diferentes regiões. Com isso, o objetivo deste trabalho foi identifi car, por meio da reação em cadeia da polimerase (PCR), a ocorrência de amostras positivas para C. fetus, oriundas de bovinos, no período de 1999 a 2010, no Rio Grande do Sul, e analisar a positividade em machos e fêmeas. Foram utilizadas 816 amostras procedentes de 37 municípios, localizados predominantemente nas mesorregiões sudoeste e centro ocidental rio-grandense, das quais 480 aspirados prepuciais (92 provenientes de duas centrais de inseminação artifi cial e 388 de estabelecimentos de criação -monta natural), 324 aspirados cervicais e conteúdo abomasal de 12 fetos bovinos abortados. Como resultado, 10,9% das amostras (89/816) foram positivas para C. fetus. Quando analisados os resultados em relação à origem das amostras, 6,5% (6/92) das coletadas de machos de centrais de inseminação foram positivas, e das obtidas de touros utilizados em monta natural, 9% (35/388). Já entre as fêmeas, esse percentual foi de 13,6% (44/324) e, nas amostras obtidas de fetos abortados, 33,3% (4/12) foram positivas. Quando analisados os 91 estabelecimentos de criação com monta natural e os 37 municípios, foram positivos 44,0% (40/91) e 63,2% (24/37), respectivamente. Palavras-chave: doença venérea, campilobacteriose genital bovina, diagnóstico, frequência. ABSTRACT Bovine genital campylobacteriosis (BGC) is an infectious disease caused by Campylobacter fetus, which

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene, 2014

Pesquisa Veterinária Brasileira, 2013