Rosanna Freyre - Academia.edu (original) (raw)

Papers by Rosanna Freyre

Horttechnology, Oct 1, 2022

Proceedings of the Florida State Horticultural Society, May 15, 2017

Ruellia consists of approximately 300 species found mostly in tropical and subtropical areas. Rue... more Ruellia consists of approximately 300 species found mostly in tropical and subtropical areas. Ruellia simplex (Mexican petunia) is an economically important ornamental in the southern United States due to its low maintenance, prolific flowering, and overall performance. However, since its introduction to Florida in the 1940s, R. simplex has naturalized in disturbed ecosystems of seven southern U.S. states, Hawaii, Puerto Rico, and the U.S. Virgin Islands. It is considered a Category I invasive by the Florida Exotic Pest Plant Council because it displaces native plant communities, disrupts ecological functions and can hybridize with native species. For these reasons, a breeding program was initiated at the University of Florida to develop cultivars with reduced fertility and with different flower colors, growth habits and ornamental traits. Breeding methods used include artificial hybridizations and ploidy manipulations. Tetraploid individuals are developed utilizing oryzalin and then intercrossed with related species to obtain hybrids with reduced female and male fertilities and no invasiveness by seed production. To date, Ruellia 'Mayan Purple', 'Mayan White', 'Mayan Pink', and 'Mayan Compact Purple' have been released and patented. The objectives of this research were to determine an optimal oryzalin treatment resulting in polyploidization of R. simplex and develop tools to efficiently determine ploidy levels. Individuals were screened for ploidy level by counting chloroplasts on the stomata and measuring stomata length, and presumed polyploids were confirmed by flow cytometry. Ruellia simplex Wright (commonly known as Mexican petunia, Mexican bluebell or Britton's petunia) has low maintenance requirements, great pest and environmental stress resistance, and prolific flowering. It has become a very popular landscape plant in the southern United States (Gilman, 1999) since its introduction to Florida sometime in the 1940s (Hupp et al., 2009). Ruellia simplex has naturalized in disturbed uplands and wetlands of seven southern U.

EDIS, Sep 30, 2012

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

EDIS, Nov 16, 2020

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

Annual Report of the Bean Improvement Cooperative. Bean Improvement Cooperative, Mar 1, 1995

Genome, Apr 1, 1991

Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida wer... more Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida were used in crosses to estimate their male and female fertility and their crossability with sweet potato, Ipomoea batatas (L.) Lam. Several important conclusions were drawn. (i) The fertility of pollen of the triploid plants was confirmed, which suggested they are truly functional 2n pollen. (ii) The average male fertility of hexaploid plants was higher than that of triploid with 2n pollen production plants. (iii) 2n egg production was observed in several triploid genotypes, (iv) From a polycross, 3079 seeds with normal shapes were produced. These represent a population of hexaploid I. trifida with a wide genetic base, and they may be important material for sweet-potato improvement, (v) A total of 3275 seeds were obtained from crosses with six sweet-potato female parents, of which only 60 germinated, indicating the existence of an interspecific barrier. The implications of 2n gametes for breeding and evolution of sweet potato are also discussed.Key words: Ipomoea batatas, Ipomoea trifida, 2n gametes, sexual polyploidization, triploid.

Euphytica, Jun 17, 2015

Artificial hybridizations (self-intra-and interspecific) were performed within and between eight ... more Artificial hybridizations (self-intra-and interspecific) were performed within and between eight species of Nolana (N. adansonii, N. aticoana, N. elegans, N. humifusa, N. ivaniana, N. laxa, N. plicata and N. rupicola). Plant material originated from three fog oases (''lomas'') locations in the Peruvian desert in Peru and two ''lomas'' locations in the Atacama desert in Chile. Self-pollinations and all possible crosses, including reciprocals, were made between three and five accessions of each species. Crosses were made in replicates of five (or ten in self-pollinations), totaling 310 self-pollinations, 480 intraspecific pollinations, and 2535 interspecific pollinations. Species were generally self-incompatible, with only one accession of N. adansonii and one accession of N. ivaniana exhibiting limited self-fertility as measured by fruit set. Intraspecific compatibility appears to be high with most crosses resulting in greater than 80 % fruit set. Success of interspecific hybridization was initially analyzed based on fruit set, mericarps per fruit and mericarp size, and confirmed by seed counts through X-ray analyses of mericarps, and seed germination. Differences in all these factors were found within seed parent species in response to hybridization with different pollen parent species. Reduction in interspecific fertility was generally seen as lower fruiting success and smaller mericarp size when compared to intraspecific hybridization, while the number of mericarps per fruit remained constant. Seed set was estimated by X-ray analysis of mericarps, revealing differences in seed counts between crosses. Mericarps were sown producing hybrid seedlings from 22 unidirectional species pairs. Previous to these studies, sexual compatibility was known to exist only between N. paradoxa and four other species. The results of these studies are a significant contribution to the current knowledge of sexual compatibility within Nolana and are important for future breeding efforts.

Hortscience, Oct 1, 2018

Additional index words. Acanthaceae, aneuploid, haploid, invasive plants, Mexican petunia, plant ... more Additional index words. Acanthaceae, aneuploid, haploid, invasive plants, Mexican petunia, plant breeding Ruellia L. (Acanthaceae) consists of 300 species of perennial herbs, shrubs, small trees, and lianas with tropical, subtropical, or temperate distribution (Tripp, 2007). Ruellia simplex Wright (commonly known as Mexican petunia, Mexican bluebell, or Britton's petunia) is a popular landscape plant in the southern United States because of its low maintenance requirements and prolific flowering (Gilman, 1999). It was introduced to Florida sometime before 1940 (Smith et al., 2016) and has naturalized in disturbed uplands and wetlands of seven southern U.

Acta horticulturae, Aug 1, 2016

Ilex crenata × I. maximowicziana 'RutHol1', Emerald Colonnade ® holly PP23,905 Emerald Colonnade ... more Ilex crenata × I. maximowicziana 'RutHol1', Emerald Colonnade ® holly PP23,905 Emerald Colonnade ® holly is the result of hybridization program at The University of Georgia in an attempt to get a faster growing, upright form of small-leaved holly. This plant is a cross between I. crenata 'Sky Pencil' and a male form of I. maximowicziana. The resulting plant is faster growing than its female parent (24 months from cutting to a finished #5), is resistant to spider mites and is female sterile so invasiveness is not an issue. Both parents and Emerald Colonnade ® have survived-5°F with no foliar or stem damage. Mature size

EDIS

Ginger, galangal, and turmeric (Figure 1) are emerging crops for Florida production. All of these... more Ginger, galangal, and turmeric (Figure 1) are emerging crops for Florida production. All of these plants are in the Zingiberaceae family and share most aspects of their production. This bulletin describes production in containers or the field under Florida conditions to help guide growers interested in ginger, turmeric, and galangal production or expanding their market. All species have been evaluated by the UF/IFAS Assessment of Non-native Plants in Florida’s Natural Areas (UF/IFAS Assessment) using the Predictive Tool (an invasion risk assessment) and all present a low risk of invasion in Florida (https://assessment.ifas.ufl.edu).

Agronomy

Excess solar radiation can negatively affect growth and rhizome yield of ginger (Zingiber officin... more Excess solar radiation can negatively affect growth and rhizome yield of ginger (Zingiber officinale) and turmeric (Curcuma longa) plants. Thus, the objective of this study was to evaluate the effect of 60% shade nets (Experiment 1) as well as white and red kaolin sprays during two production stages (early establishment vs. entire cycle) (Experiment 2) on field-grown ginger and turmeric plants. In Experiment 1, plants were propagated from seed rhizomes (R) or second-generation rhizomes from tissue-cultured plants (2GR), while only R were used in Experiment 2. There were no differences in rhizome yield in response to shade in Experiment 1, with mean values of 644 and 692 g in ginger and turmeric, respectively. Overall, 2GR ginger plants produced a higher rhizome yield (880 g) than R plants (425 g), but no yield differences were measured in turmeric. In Experiment 2, for both species and regardless of kaolin color, sprays applied during the entire cycle increased photosynthesis and st...

Acta Horticulturae, 2019

Edible ginger (Zingiber officinale) has potential as a greenhouse crop with multiple uses, includ... more Edible ginger (Zingiber officinale) has potential as a greenhouse crop with multiple uses, including food, beverage, medicinal, and ornamental. In this study we compared plants of edible ginger from different starting material (seed rhizomes, or micro-propagated plantlets planted on two different dates), planted in two container sizes (5.7 or 50.5 L), and grown under either natural photoperiod or long days with night interruption lighting under greenhouse conditions. Rhizome-grown plants and plantlets (early planting) in both container sizes had similar rhizome fresh mass under natural or long days, but rhizome mass was lower for the plantlets (late planting) grown under long days. Some plants grown from tissue culture had thin rhizomes that would not be ideal for sale as fresh produce. Thus, micro-propagated plantlets may be most effective as clean stock material to produce seed rhizomes for second-year production. Under long days, rhizome moisture content was high compared with plants grown under natural days, and high moisture content would be preferable for the fresh market. For higher rhizome yield, it may be best to grow plants under long days, and then force dormancy by either drying plants or providing short days.

Acta Horticulturae, 2015

Wild Ruellia simplex (Mexican petunia) is a popular landscape plant in the southern USA. This int... more Wild Ruellia simplex (Mexican petunia) is a popular landscape plant in the southern USA. This introduced plant is highly fertile, has escaped from cultivation and become invasive in natural areas. For several years 'Purple Showers' was the only existing sterile cultivar. Our breeding objective has been to develop sterile Ruellia cultivars with different flower colors and growth habits. Breeding approaches are ploidy manipulation and hybridization. Selected hybrids are evaluated in replicated trials in Florida. Female and male fertilities are determined by collection of fruits produced by open pollination, by manual hybridizations in the greenhouse, and by evaluation of pollen staining. From 2011 to 2013 the first three sterile hybrids named 'Mayan Purple', 'Mayan White' and 'Mayan Pink' were released, and are commercially available now. The 'Mayan' series was evaluated in several research and commercial trials in the USA demonstrating very good to excellent performance in terms of growth habit and flowering. In another research topic, genetics and anthocyanins responsible for flower color were studied in R. simplex. An F 1 population was obtained from crossing a maternal individual with white corolla with purple throat (WP), and a paternal individual with pink corolla and dark pink throat (PK). All plants in the F 1 generation had purple flowers (P). The F 2 generation segregated 94P:30PK:24WP:5WPK (WPK is white corolla and pink throat). These data were separated into two groups: the corolla color fit a 9:3:4 recessive epistasis interaction (P=0.22) whereas the throat color fit a 3:1 ratio (P=0.54). HPLC analyses indicated that delphinidin derivatives conferred purple corolla color, while pelargonidin derivatives were responsible for the pink corolla color. Purple throat color resulted from delphinidin derivatives whereas the pink color was caused by peonidin derivatives.

Acta horticulturae, Aug 1, 2016

… Potato Center (CIP), March 5-9 …, 1991

... Electrophoretic evidence and plant systematics. Annals of Missouri Botanical Garden 64: 161-1... more ... Electrophoretic evidence and plant systematics. Annals of Missouri Botanical Garden 64: 161-180. Guri, A. and KC Sink. 1988. ... ATLANTIC YUK GOLD NORLAND AL FROST Potato Cultivars 1. Segmation of potato cultivars based upon 13 isozyme loci. 175 Page 184.

Horttechnology, Oct 1, 2022

Proceedings of the Florida State Horticultural Society, May 15, 2017

Ruellia consists of approximately 300 species found mostly in tropical and subtropical areas. Rue... more Ruellia consists of approximately 300 species found mostly in tropical and subtropical areas. Ruellia simplex (Mexican petunia) is an economically important ornamental in the southern United States due to its low maintenance, prolific flowering, and overall performance. However, since its introduction to Florida in the 1940s, R. simplex has naturalized in disturbed ecosystems of seven southern U.S. states, Hawaii, Puerto Rico, and the U.S. Virgin Islands. It is considered a Category I invasive by the Florida Exotic Pest Plant Council because it displaces native plant communities, disrupts ecological functions and can hybridize with native species. For these reasons, a breeding program was initiated at the University of Florida to develop cultivars with reduced fertility and with different flower colors, growth habits and ornamental traits. Breeding methods used include artificial hybridizations and ploidy manipulations. Tetraploid individuals are developed utilizing oryzalin and then intercrossed with related species to obtain hybrids with reduced female and male fertilities and no invasiveness by seed production. To date, Ruellia 'Mayan Purple', 'Mayan White', 'Mayan Pink', and 'Mayan Compact Purple' have been released and patented. The objectives of this research were to determine an optimal oryzalin treatment resulting in polyploidization of R. simplex and develop tools to efficiently determine ploidy levels. Individuals were screened for ploidy level by counting chloroplasts on the stomata and measuring stomata length, and presumed polyploids were confirmed by flow cytometry. Ruellia simplex Wright (commonly known as Mexican petunia, Mexican bluebell or Britton's petunia) has low maintenance requirements, great pest and environmental stress resistance, and prolific flowering. It has become a very popular landscape plant in the southern United States (Gilman, 1999) since its introduction to Florida sometime in the 1940s (Hupp et al., 2009). Ruellia simplex has naturalized in disturbed uplands and wetlands of seven southern U.

EDIS, Sep 30, 2012

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

EDIS, Nov 16, 2020

The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution author... more The Institute of Food and Agricultural Sciences (IFAS) is an Equal Opportunity Institution authorized to provide research, educational information and other services only to individuals and institutions that function with non-discrimination with respect to race, creed, color, religion, age, disability, sex, sexual orientation, marital status, national origin, political opinions or affiliations.

Annual Report of the Bean Improvement Cooperative. Bean Improvement Cooperative, Mar 1, 1995

Genome, Apr 1, 1991

Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida wer... more Twenty-two synthetic hexaploids and 33 triploids with 2n pollen production of Ipomoea trifida were used in crosses to estimate their male and female fertility and their crossability with sweet potato, Ipomoea batatas (L.) Lam. Several important conclusions were drawn. (i) The fertility of pollen of the triploid plants was confirmed, which suggested they are truly functional 2n pollen. (ii) The average male fertility of hexaploid plants was higher than that of triploid with 2n pollen production plants. (iii) 2n egg production was observed in several triploid genotypes, (iv) From a polycross, 3079 seeds with normal shapes were produced. These represent a population of hexaploid I. trifida with a wide genetic base, and they may be important material for sweet-potato improvement, (v) A total of 3275 seeds were obtained from crosses with six sweet-potato female parents, of which only 60 germinated, indicating the existence of an interspecific barrier. The implications of 2n gametes for breeding and evolution of sweet potato are also discussed.Key words: Ipomoea batatas, Ipomoea trifida, 2n gametes, sexual polyploidization, triploid.

Euphytica, Jun 17, 2015

Artificial hybridizations (self-intra-and interspecific) were performed within and between eight ... more Artificial hybridizations (self-intra-and interspecific) were performed within and between eight species of Nolana (N. adansonii, N. aticoana, N. elegans, N. humifusa, N. ivaniana, N. laxa, N. plicata and N. rupicola). Plant material originated from three fog oases (''lomas'') locations in the Peruvian desert in Peru and two ''lomas'' locations in the Atacama desert in Chile. Self-pollinations and all possible crosses, including reciprocals, were made between three and five accessions of each species. Crosses were made in replicates of five (or ten in self-pollinations), totaling 310 self-pollinations, 480 intraspecific pollinations, and 2535 interspecific pollinations. Species were generally self-incompatible, with only one accession of N. adansonii and one accession of N. ivaniana exhibiting limited self-fertility as measured by fruit set. Intraspecific compatibility appears to be high with most crosses resulting in greater than 80 % fruit set. Success of interspecific hybridization was initially analyzed based on fruit set, mericarps per fruit and mericarp size, and confirmed by seed counts through X-ray analyses of mericarps, and seed germination. Differences in all these factors were found within seed parent species in response to hybridization with different pollen parent species. Reduction in interspecific fertility was generally seen as lower fruiting success and smaller mericarp size when compared to intraspecific hybridization, while the number of mericarps per fruit remained constant. Seed set was estimated by X-ray analysis of mericarps, revealing differences in seed counts between crosses. Mericarps were sown producing hybrid seedlings from 22 unidirectional species pairs. Previous to these studies, sexual compatibility was known to exist only between N. paradoxa and four other species. The results of these studies are a significant contribution to the current knowledge of sexual compatibility within Nolana and are important for future breeding efforts.

Hortscience, Oct 1, 2018

Additional index words. Acanthaceae, aneuploid, haploid, invasive plants, Mexican petunia, plant ... more Additional index words. Acanthaceae, aneuploid, haploid, invasive plants, Mexican petunia, plant breeding Ruellia L. (Acanthaceae) consists of 300 species of perennial herbs, shrubs, small trees, and lianas with tropical, subtropical, or temperate distribution (Tripp, 2007). Ruellia simplex Wright (commonly known as Mexican petunia, Mexican bluebell, or Britton's petunia) is a popular landscape plant in the southern United States because of its low maintenance requirements and prolific flowering (Gilman, 1999). It was introduced to Florida sometime before 1940 (Smith et al., 2016) and has naturalized in disturbed uplands and wetlands of seven southern U.

Acta horticulturae, Aug 1, 2016

Ilex crenata × I. maximowicziana 'RutHol1', Emerald Colonnade ® holly PP23,905 Emerald Colonnade ... more Ilex crenata × I. maximowicziana 'RutHol1', Emerald Colonnade ® holly PP23,905 Emerald Colonnade ® holly is the result of hybridization program at The University of Georgia in an attempt to get a faster growing, upright form of small-leaved holly. This plant is a cross between I. crenata 'Sky Pencil' and a male form of I. maximowicziana. The resulting plant is faster growing than its female parent (24 months from cutting to a finished #5), is resistant to spider mites and is female sterile so invasiveness is not an issue. Both parents and Emerald Colonnade ® have survived-5°F with no foliar or stem damage. Mature size

EDIS

Ginger, galangal, and turmeric (Figure 1) are emerging crops for Florida production. All of these... more Ginger, galangal, and turmeric (Figure 1) are emerging crops for Florida production. All of these plants are in the Zingiberaceae family and share most aspects of their production. This bulletin describes production in containers or the field under Florida conditions to help guide growers interested in ginger, turmeric, and galangal production or expanding their market. All species have been evaluated by the UF/IFAS Assessment of Non-native Plants in Florida’s Natural Areas (UF/IFAS Assessment) using the Predictive Tool (an invasion risk assessment) and all present a low risk of invasion in Florida (https://assessment.ifas.ufl.edu).

Agronomy

Excess solar radiation can negatively affect growth and rhizome yield of ginger (Zingiber officin... more Excess solar radiation can negatively affect growth and rhizome yield of ginger (Zingiber officinale) and turmeric (Curcuma longa) plants. Thus, the objective of this study was to evaluate the effect of 60% shade nets (Experiment 1) as well as white and red kaolin sprays during two production stages (early establishment vs. entire cycle) (Experiment 2) on field-grown ginger and turmeric plants. In Experiment 1, plants were propagated from seed rhizomes (R) or second-generation rhizomes from tissue-cultured plants (2GR), while only R were used in Experiment 2. There were no differences in rhizome yield in response to shade in Experiment 1, with mean values of 644 and 692 g in ginger and turmeric, respectively. Overall, 2GR ginger plants produced a higher rhizome yield (880 g) than R plants (425 g), but no yield differences were measured in turmeric. In Experiment 2, for both species and regardless of kaolin color, sprays applied during the entire cycle increased photosynthesis and st...

Acta Horticulturae, 2019

Edible ginger (Zingiber officinale) has potential as a greenhouse crop with multiple uses, includ... more Edible ginger (Zingiber officinale) has potential as a greenhouse crop with multiple uses, including food, beverage, medicinal, and ornamental. In this study we compared plants of edible ginger from different starting material (seed rhizomes, or micro-propagated plantlets planted on two different dates), planted in two container sizes (5.7 or 50.5 L), and grown under either natural photoperiod or long days with night interruption lighting under greenhouse conditions. Rhizome-grown plants and plantlets (early planting) in both container sizes had similar rhizome fresh mass under natural or long days, but rhizome mass was lower for the plantlets (late planting) grown under long days. Some plants grown from tissue culture had thin rhizomes that would not be ideal for sale as fresh produce. Thus, micro-propagated plantlets may be most effective as clean stock material to produce seed rhizomes for second-year production. Under long days, rhizome moisture content was high compared with plants grown under natural days, and high moisture content would be preferable for the fresh market. For higher rhizome yield, it may be best to grow plants under long days, and then force dormancy by either drying plants or providing short days.

Acta Horticulturae, 2015

Wild Ruellia simplex (Mexican petunia) is a popular landscape plant in the southern USA. This int... more Wild Ruellia simplex (Mexican petunia) is a popular landscape plant in the southern USA. This introduced plant is highly fertile, has escaped from cultivation and become invasive in natural areas. For several years 'Purple Showers' was the only existing sterile cultivar. Our breeding objective has been to develop sterile Ruellia cultivars with different flower colors and growth habits. Breeding approaches are ploidy manipulation and hybridization. Selected hybrids are evaluated in replicated trials in Florida. Female and male fertilities are determined by collection of fruits produced by open pollination, by manual hybridizations in the greenhouse, and by evaluation of pollen staining. From 2011 to 2013 the first three sterile hybrids named 'Mayan Purple', 'Mayan White' and 'Mayan Pink' were released, and are commercially available now. The 'Mayan' series was evaluated in several research and commercial trials in the USA demonstrating very good to excellent performance in terms of growth habit and flowering. In another research topic, genetics and anthocyanins responsible for flower color were studied in R. simplex. An F 1 population was obtained from crossing a maternal individual with white corolla with purple throat (WP), and a paternal individual with pink corolla and dark pink throat (PK). All plants in the F 1 generation had purple flowers (P). The F 2 generation segregated 94P:30PK:24WP:5WPK (WPK is white corolla and pink throat). These data were separated into two groups: the corolla color fit a 9:3:4 recessive epistasis interaction (P=0.22) whereas the throat color fit a 3:1 ratio (P=0.54). HPLC analyses indicated that delphinidin derivatives conferred purple corolla color, while pelargonidin derivatives were responsible for the pink corolla color. Purple throat color resulted from delphinidin derivatives whereas the pink color was caused by peonidin derivatives.

Acta horticulturae, Aug 1, 2016

… Potato Center (CIP), March 5-9 …, 1991

... Electrophoretic evidence and plant systematics. Annals of Missouri Botanical Garden 64: 161-1... more ... Electrophoretic evidence and plant systematics. Annals of Missouri Botanical Garden 64: 161-180. Guri, A. and KC Sink. 1988. ... ATLANTIC YUK GOLD NORLAND AL FROST Potato Cultivars 1. Segmation of potato cultivars based upon 13 isozyme loci. 175 Page 184.