Rosaura Hernández Valles - Academia.edu (original) (raw)
Papers by Rosaura Hernández Valles
Medical Mycology, 2003
Ajoene and 5-fluorouracil (5-FU) are compounds that have shown in-vitro activity against Cladophi... more Ajoene and 5-fluorouracil (5-FU) are compounds that have shown in-vitro activity against Cladophialophora carrionii, an important etiologic agent of chromoblastomycosis. An open comparative trial was conducted to assess safety and effectiveness of topical ajoene and 5-FU in the treatment of localized chromoblastomycosis. Thirty-seven patients with a clinically and mycologically confirmed diagnosis were randomly distributed into two groups allocated to ajoene (0.5% gel; n 0/19) or 5-FU (1% cream; n 0/18). Topical treatment was applied to localized lesions (5/2.5-cm diameter) once a day, with occlusion, for 12Á/16 weeks. Complete clinical and mycological remission was achieved in 14/19 patients (74%) treated with ajoene and 14/18 patients (78%) treated with 5-FU. All 5-FU-treated patients developed a post-treatment scar at the site of the lesion, while ajoene-treated patients showed only a slight depigmentation of the skin. The differences observed in cure rate between ajoene and 5-FU are not statistically significant. Follow-up of all patients for 4 years revealed no relapses in the ajoene-treated group, while one patient in the 5-FU-treated group had a relapse 6 months after the end of therapy. This trial represents the first clinical use of ajoene in the control of a deep mycosis.
Medical Mycology, 2006
The present paper describes 22 cases of chromoblastomycosis (CBM) caused by Cladophialophora carr... more The present paper describes 22 cases of chromoblastomycosis (CBM) caused by Cladophialophora carrionii in children and adolescents (2Á19 years old). The patients were seen between 1992 and 2004 and all resided in a CBM endemic area in the semi-arid zone of the Falcó n state, Venezuela. Twelve of the 22 patients (54.55%) had close relatives who also had CBM and 19 (86.36%) were male. Lesions consisted of erythematous papules with desquamation or squamous plaques (0.12Á14.19cm in diameter), located primarily on the upper limbs (77.27% of patients). Thirteen of the patients were treated with topical 5fluorouracil (5-FU; 1% cream), seven with topical ajoene (0.5% gel) and two had electrodesiccation and/or fulguration. Two patients who did not respond to 5-FU were treated with oral itraconazole (100 mg/day for 1 month). Complete clinical and mycological remission was achieved in 17/20 (85%) of the patients treated with 5-FC, ajoene and electrodesiccation and/or fulguration. In addition, similar results were obtained with the two patients who received itraconazole therapy. These cases emphasize the importance of early diagnosis in difficult-to-treat mycotic diseases such as CBM. By early intervention we were able to employ topical treatment with a minimum of adverse effects to achieve a high percentage of favorable therapeutic responses. The patients were thus able to avoid the evolution of the chronic, deforming and incapacitation clinical manifestations associated with CBM.
Chromoblastomycosis (CBM) is a chronic, localized deep fungal infection of the skin and subcutane... more Chromoblastomycosis (CBM) is a chronic, localized deep fungal infection of the skin and subcutaneous tissues, caused by dematiaceous (black) fungi. We describe the case of 62 years-old-male patient, from a rural town, with seborrheic dermatitis for more than 10 years, and a desquamative lesion in the right ear, where no diagnosis of CBM was never made. Direct exam with KOH, macro and microscopic culture characteristics, epidemiological and clinical findings confirmed the diagnosis of CBM by Cladophialophora carrionii, the first case report of auricular lesions published in Venezuela
Investigación Clínica, 2019
The identification of Candida species and their antifungal susceptibility is important for the tr... more The identification of Candida species and their antifungal susceptibility is important for the treatment of infected patients. The aim was to determine the susceptibility to fluconazole and voriconazole in isolates from oral colonization of Candida spp. in HIV patients. From the 135 patients studied, 33.3% were females and 66.7% males, with a mean age of 36.6 years and 83.7% of them were under treatment. The identification of the specie was performed by the API20CAUX ® test and the antifungal susceptibility was determined by the disc diffusion test. Strains of C. parapsilosis and C. krusei were used as quality controls. The Candida species identificated were: C. parapsilosis complex 52%, C. albicans complex 36% and C. famata 12%. 60% of the isolates were susceptible to fluconazole and 40% were dose-dependent. All were susceptible to voriconazole. In this study, none of the patients had oropharyngeal candidiasis however, 18.5% had Candida spp. colonization, this percentage being below other studies in HIV carriers. We found a high proportion of nonalbicans species but no difference in the CD4+ counts between patients.
Nosocomial infection by Candida species are an important cause of morbidity and mortality, especi... more Nosocomial infection by Candida species are an important cause of morbidity and mortality, especially in seriously ill patients, and it is important to establish the infection source in hospital environments. The purpose of this study was to isolate and identify Candida species in medical equipments, furniture, and hands of staff working in intensive care units and evaluate their susceptibility to fluconazole and voriconazole. Samples were obtained with sterile swabs humidified with brain-heart broth supplemented with chloramphenicol. Identification was done through growth characteristics in chromogenic agar and by conventional methods; fluconazol and voriconazole susceptibility was evaluated by the disk diffusion method (CLSI M44-A). Twenty-six (68.4%) of the isolates were obtained from furniture and medical equipments and 12 (31.6%) from the hands of the staff. The most frequent species was C. parapsilosis (55.4%), followed by C. tropicalis, C. famata, C. glabrata and C. guillierm...
The eco-environmental characteristics of populations from the Falcon Municipality of the Paraguan... more The eco-environmental characteristics of populations from the Falcon Municipality of the Paraguana Peninsula were studied, and soil samples were taken for identification of isolates suggestive of Coccidioides spp., through direct exam of tissue samples from mice infected with this fungus. The eco-environmental characteristics of the municipality and the chemical analysis of the soil favor the presence of this fungus in this zone. From the soil sample cultures we obtained an isolate suggestive of Coccidioides spp, but the experimental animal study did not reveal spherule formation. The existence of a Coccidioidomycosis risk area at the Paraguana Peninsula was confirmed.
Investigacion Clinica, 2015
La coccidioidomicosis es una micosis sistemica endemica del continente americano, causada por un ... more La coccidioidomicosis es una micosis sistemica endemica del continente americano, causada por un hongo dimorfico. La inhalacion de artroconidios transportados por el viento permite la colonizacion del tejido pulmonar produciendo la micosis. El diagnostico se realiza a traves del estudio epidemiologico y micologico, complementandose con el histopatologico, inmunitario y molecular. En Venezuela ha sido reportada desde 1948 cuando el Dr. Humberto Campins describio el primer caso en Barquisimeto, estado Lara. Las micosis en Venezuela no son enfermedades de denuncia obligatoria por lo que existe un grave subregistro en las estadisticas anuales del pais, sin embargo, los Grupos de Trabajo en Micologia logran mantener la data de los casos. Los datos aportados acerca de las variables bioclimaticas y ambientales de las zonas endemicas pueden contribuir a la busqueda del nicho ecologico del hongo, para asi apoyar la vigilancia eco-epidemiologica de los casos clinicos y la pesquisa de casos su...
Investigacion Clinica, 2015
Las especies de Candida distintas a C. albicans se describen con frecuencia como agentes causales... more Las especies de Candida distintas a C. albicans se describen con frecuencia como agentes causales de micosis superficial y presentan una mayor resistencia al tratamiento con los azoles. Con la finalidad de determinar la distribucion de especies y la susceptibilidad antifungica in vitro de Candida spp., se realizo un estudio ambispectivo donde se analizaron 18 aislados de levaduras obtenidas de muestras de pacientes con diagnostico de micosis superficial. La identificacion taxonomica se realizo mediante la visualizacion de las caracteristicas macroscopicas de crecimiento en agar cromogenico y por metodos convencionales. La susceptibilidad a fluconazol y voriconazol se evaluo por el metodo de difusion en disco. El 88,8% de los aislados provenia de muestras de unas. C. parapsilosis fue la especie mas frecuente, seguida de C. tropicalis , C. albicans y C. krusei , lo cual confirmo el predominio de especies no albicans como causa de micosis superficial. El patron de susceptibilidad a flu...
Revista Iberoamericana De Micologia, 2013
Revista De La Sociedad Venezolana De Microbiologia, 2013
Resumen: El objetivo de este trabajo fue determinar la frecuencia de aislamiento de Candida spp. ... more Resumen: El objetivo de este trabajo fue determinar la frecuencia de aislamiento de Candida spp. en muestras clínicas de pacientes ingresados en una unidad de cuidados intensivos y evaluar su perfil de susceptibilidad in vitro a fluconazol y voriconazol. Se evaluaron 60 pacientes, procesándose 194 muestras: 98 hemocultivos, 48 urocultivos cuantitativos, 46 secreciones traqueales y 2 puntas de catéter, mediante siembra en agar cromogénico para Candida. El género y especie fue confirmado mediante las características macroscópicas de crecimiento, producción de clamidoconidias, hidrólisis de la urea y asimilación de carbohidratos. Para la evaluación de la susceptibilidad antifúngica se utilizó el método de difusión en disco M44-A del Clinical and Laboratory Standard Institute. Se aislaron 37 levaduras (73,0% de urocultivo en recuento significativo, 24,3% de secreción traqueal y 2,7% de punta de catéter). C. tropicalis fue la especie más frecuente (43,2%) seguida de C. glabrata (21,6%) y C. albicans (13,5%), evidenciándose una distribución diferente a la reportada en la literatura. El 73% de los aislados fue sensible a fluconazol y el 89,2% al voriconazol. La frecuencia observada de candiduria, sugiere que el urocultivo cuantitativo pudiera emplearse como estudio rutinario en pacientes recluidos en UCI, a fin de establecer estrategias terapéuticas y profilácticas adecuadas.
Revista De La Sociedad Venezolana De Microbiologia Num 1 Vol 34, 2015
Artículo original Aislamiento de Candida spp. en ambiente y personal que labora en una unidad de ... more Artículo original Aislamiento de Candida spp. en ambiente y personal que labora en una unidad de cuidados intensivos
Investigación clínica, 2015
Candida species other than C. albicans are often described as causative agents of superficial myc... more Candida species other than C. albicans are often described as causative agents of superficial mycosis and are more resistant to treatment with azoles. In order to determine the distribution of species and in vitro antifungal susceptibility of Candida spp., one ambispective study, which analyzed 18 yeast isolates obtained from samples from patients diagnosed with superficial mycosis, was performed. Taxonomic identification was performed by macroscopic visualization of the growth characteristics in chromogenic agar and by conventional methods. The susceptibility to fluconazole and voriconazole was evaluated by the disc diffusion method. Most of the isolates (88.8%), came from nail samples. C. parapsilosis was the most common species, followed by C. tropicalis, C. albicans and C. krusei, which confirmed the prevalence of non-albicans species as a cause of superficial mycoses. The pattern of susceptibility to fluconazole and voriconazole was similar: all isolates of C. parapsilosis and ...
Revista Iberoamericana de Micología, 2013
Antecedentes: En 1984 se crearon los Grupos de Trabajo en Micología de Venezuela (GTMV), proporci... more Antecedentes: En 1984 se crearon los Grupos de Trabajo en Micología de Venezuela (GTMV), proporcionando un abordaje novedoso al estudio de las micosis, en especial las micosis endémicas. Objetivos: Conocer los aportes en el estudio sistemático de las micosis en Venezuela durante 26 años de labor de los GTMV. Métodos: Se realizó revisión de la casuística publicada por los GTMV en el Boletín Informativo Las Micosis en Venezuela desde 1984 hasta 2010. Resultados: Se obtuvieron 36.968 diagnósticos de micosis superficiales, 1.989 de profundas sistémicas y 822 de profundas localizadas. La dermatofitosis fue la patología superficial más frecuente, paracoccidioidomicosis e histoplasmosis las profundas sistémicas, y cromoblastomicosis la profunda localizada. Se realizó la distribución geográfica de los casos de micosis profundas, pudiendo delimitar las zonas endémicas. Discusión: Las micosis superficiales constituyen un problema de salud pública por su alta morbilidad y pueden ser responsables de epidemias en grupos de riesgo. La paracoccidioidomicosis y la histoplamosis se reportaron con mayor frecuencia, incluso antes de haberse conformado los GTMV. El número de cromoblastomicosis y esporotricosis en Venezuela supera a lo reportado en otros países. Los GTMV han contribuido al conocimiento de la incidencia y prevalencia las micosis en el país, además de su divulgación como problema de salud pública, siendo un aporte invaluable que debe mantenerse en el tiempo, tratando no solo de reportar la casuística anual, sino también detallar aspectos clínico-epidemiológicos que permitan realizar seguimiento de la evolución de estas patologías.
Rev. Soc. Ven. …, 2011
... Leyla Humbría García a, *, Rosaura Hernández Valles a , Maigualida Pérez Blanco a , Leila Gar... more ... Leyla Humbría García a, *, Rosaura Hernández Valles a , Maigualida Pérez Blanco a , Leila García Hernández a , Mireya Mendoza b , Edgar ... Mondragón-González R, Méndez-Tovar LJ, Bernal-Vázquez E, Hernández-Hernández F, López-Martínez R, Manzano-Gayosso P, y col ...
Rev. Soc. Ven. Microbiol, 2010
cultivaron muestras de suelo para identificación de los aislados sugestivos de Coccidioides spp.,... more cultivaron muestras de suelo para identificación de los aislados sugestivos de Coccidioides spp., mediante examen directo de las muestras de tejido de ratones inoculados con el hongo. Las características ecoambientales del municipio y el análisis químico del suelo favorecen la presencia del hongo en esta zona. Del cultivo de suelos se obtuvo un aislado sugestivo de Coccidioides spp., pero el estudio en animales
Revista de la …, 2011
Resumen: La infección por el Virus de Hepatitis B (VHB) representa un problema de salud pública a... more Resumen: La infección por el Virus de Hepatitis B (VHB) representa un problema de salud pública a escala mundial, estimándose que más de dos millardos de personas en el mundo se han infectado, de los cuales aproximadamente 350 millones son portadores crónicos del virus. Cerca de un millón de muertes al año están relacionadas con hepatocarcinoma primario asociado a la infección por VHB, lo que hace a éste virus la cuarta causa de muerte por enfermedades infecciosas a nivel mundial. La replicación del VHB posee un potencial de variabilidad genética mayor que la de los virus ADN en general favoreciendo la aparición de mutantes naturales generadas por sustituciones puntuales, por reordenamiento de genes, o por cambios en los marcos de lectura denominados genotipos. La variabilidad genética podría estar asociada con las diferentes vías de transmisión, resistencia al interferón o progresión hacia el desarrollo de carcinoma hepatocelular.
Investigación clínica, 1996
This is a report of three cases of mycetome studied in our laboratory during the last four years.... more This is a report of three cases of mycetome studied in our laboratory during the last four years. We also informed about the evolution of the first case produced by Pyrenochaeta romeroi nova species reported by Borelli in 1959. All the patients were farmer men, 18, 42 and 54 years old, from the semiarid and subhumid zones of Falcon State, located on the northwest region of Venezuela. Clinically, all patients presented the mycetome syndrome (subcutaneous edema, sinus tract and "granules"), with a evolution of six months to three years. The lesions were localized in the inferior limbs in two of the patients and in the thorax in one of them. The clinical diagnose was confirmed by microbiological and mycological studies, isolating Nocardia brasiliensis in two cases and Madurella grisea in one. Two patients treated with Sulfas showed remission of the disease in a two years follow up. Including this report, 15 cases of mycetome have been reported in the Falcon State (Venezuela) ...
Medical Mycology, 2003
Ajoene and 5-fluorouracil (5-FU) are compounds that have shown in-vitro activity against Cladophi... more Ajoene and 5-fluorouracil (5-FU) are compounds that have shown in-vitro activity against Cladophialophora carrionii, an important etiologic agent of chromoblastomycosis. An open comparative trial was conducted to assess safety and effectiveness of topical ajoene and 5-FU in the treatment of localized chromoblastomycosis. Thirty-seven patients with a clinically and mycologically confirmed diagnosis were randomly distributed into two groups allocated to ajoene (0.5% gel; n 0/19) or 5-FU (1% cream; n 0/18). Topical treatment was applied to localized lesions (5/2.5-cm diameter) once a day, with occlusion, for 12Á/16 weeks. Complete clinical and mycological remission was achieved in 14/19 patients (74%) treated with ajoene and 14/18 patients (78%) treated with 5-FU. All 5-FU-treated patients developed a post-treatment scar at the site of the lesion, while ajoene-treated patients showed only a slight depigmentation of the skin. The differences observed in cure rate between ajoene and 5-FU are not statistically significant. Follow-up of all patients for 4 years revealed no relapses in the ajoene-treated group, while one patient in the 5-FU-treated group had a relapse 6 months after the end of therapy. This trial represents the first clinical use of ajoene in the control of a deep mycosis.
Medical Mycology, 2006
The present paper describes 22 cases of chromoblastomycosis (CBM) caused by Cladophialophora carr... more The present paper describes 22 cases of chromoblastomycosis (CBM) caused by Cladophialophora carrionii in children and adolescents (2Á19 years old). The patients were seen between 1992 and 2004 and all resided in a CBM endemic area in the semi-arid zone of the Falcó n state, Venezuela. Twelve of the 22 patients (54.55%) had close relatives who also had CBM and 19 (86.36%) were male. Lesions consisted of erythematous papules with desquamation or squamous plaques (0.12Á14.19cm in diameter), located primarily on the upper limbs (77.27% of patients). Thirteen of the patients were treated with topical 5fluorouracil (5-FU; 1% cream), seven with topical ajoene (0.5% gel) and two had electrodesiccation and/or fulguration. Two patients who did not respond to 5-FU were treated with oral itraconazole (100 mg/day for 1 month). Complete clinical and mycological remission was achieved in 17/20 (85%) of the patients treated with 5-FC, ajoene and electrodesiccation and/or fulguration. In addition, similar results were obtained with the two patients who received itraconazole therapy. These cases emphasize the importance of early diagnosis in difficult-to-treat mycotic diseases such as CBM. By early intervention we were able to employ topical treatment with a minimum of adverse effects to achieve a high percentage of favorable therapeutic responses. The patients were thus able to avoid the evolution of the chronic, deforming and incapacitation clinical manifestations associated with CBM.
Chromoblastomycosis (CBM) is a chronic, localized deep fungal infection of the skin and subcutane... more Chromoblastomycosis (CBM) is a chronic, localized deep fungal infection of the skin and subcutaneous tissues, caused by dematiaceous (black) fungi. We describe the case of 62 years-old-male patient, from a rural town, with seborrheic dermatitis for more than 10 years, and a desquamative lesion in the right ear, where no diagnosis of CBM was never made. Direct exam with KOH, macro and microscopic culture characteristics, epidemiological and clinical findings confirmed the diagnosis of CBM by Cladophialophora carrionii, the first case report of auricular lesions published in Venezuela
Investigación Clínica, 2019
The identification of Candida species and their antifungal susceptibility is important for the tr... more The identification of Candida species and their antifungal susceptibility is important for the treatment of infected patients. The aim was to determine the susceptibility to fluconazole and voriconazole in isolates from oral colonization of Candida spp. in HIV patients. From the 135 patients studied, 33.3% were females and 66.7% males, with a mean age of 36.6 years and 83.7% of them were under treatment. The identification of the specie was performed by the API20CAUX ® test and the antifungal susceptibility was determined by the disc diffusion test. Strains of C. parapsilosis and C. krusei were used as quality controls. The Candida species identificated were: C. parapsilosis complex 52%, C. albicans complex 36% and C. famata 12%. 60% of the isolates were susceptible to fluconazole and 40% were dose-dependent. All were susceptible to voriconazole. In this study, none of the patients had oropharyngeal candidiasis however, 18.5% had Candida spp. colonization, this percentage being below other studies in HIV carriers. We found a high proportion of nonalbicans species but no difference in the CD4+ counts between patients.
Nosocomial infection by Candida species are an important cause of morbidity and mortality, especi... more Nosocomial infection by Candida species are an important cause of morbidity and mortality, especially in seriously ill patients, and it is important to establish the infection source in hospital environments. The purpose of this study was to isolate and identify Candida species in medical equipments, furniture, and hands of staff working in intensive care units and evaluate their susceptibility to fluconazole and voriconazole. Samples were obtained with sterile swabs humidified with brain-heart broth supplemented with chloramphenicol. Identification was done through growth characteristics in chromogenic agar and by conventional methods; fluconazol and voriconazole susceptibility was evaluated by the disk diffusion method (CLSI M44-A). Twenty-six (68.4%) of the isolates were obtained from furniture and medical equipments and 12 (31.6%) from the hands of the staff. The most frequent species was C. parapsilosis (55.4%), followed by C. tropicalis, C. famata, C. glabrata and C. guillierm...
The eco-environmental characteristics of populations from the Falcon Municipality of the Paraguan... more The eco-environmental characteristics of populations from the Falcon Municipality of the Paraguana Peninsula were studied, and soil samples were taken for identification of isolates suggestive of Coccidioides spp., through direct exam of tissue samples from mice infected with this fungus. The eco-environmental characteristics of the municipality and the chemical analysis of the soil favor the presence of this fungus in this zone. From the soil sample cultures we obtained an isolate suggestive of Coccidioides spp, but the experimental animal study did not reveal spherule formation. The existence of a Coccidioidomycosis risk area at the Paraguana Peninsula was confirmed.
Investigacion Clinica, 2015
La coccidioidomicosis es una micosis sistemica endemica del continente americano, causada por un ... more La coccidioidomicosis es una micosis sistemica endemica del continente americano, causada por un hongo dimorfico. La inhalacion de artroconidios transportados por el viento permite la colonizacion del tejido pulmonar produciendo la micosis. El diagnostico se realiza a traves del estudio epidemiologico y micologico, complementandose con el histopatologico, inmunitario y molecular. En Venezuela ha sido reportada desde 1948 cuando el Dr. Humberto Campins describio el primer caso en Barquisimeto, estado Lara. Las micosis en Venezuela no son enfermedades de denuncia obligatoria por lo que existe un grave subregistro en las estadisticas anuales del pais, sin embargo, los Grupos de Trabajo en Micologia logran mantener la data de los casos. Los datos aportados acerca de las variables bioclimaticas y ambientales de las zonas endemicas pueden contribuir a la busqueda del nicho ecologico del hongo, para asi apoyar la vigilancia eco-epidemiologica de los casos clinicos y la pesquisa de casos su...
Investigacion Clinica, 2015
Las especies de Candida distintas a C. albicans se describen con frecuencia como agentes causales... more Las especies de Candida distintas a C. albicans se describen con frecuencia como agentes causales de micosis superficial y presentan una mayor resistencia al tratamiento con los azoles. Con la finalidad de determinar la distribucion de especies y la susceptibilidad antifungica in vitro de Candida spp., se realizo un estudio ambispectivo donde se analizaron 18 aislados de levaduras obtenidas de muestras de pacientes con diagnostico de micosis superficial. La identificacion taxonomica se realizo mediante la visualizacion de las caracteristicas macroscopicas de crecimiento en agar cromogenico y por metodos convencionales. La susceptibilidad a fluconazol y voriconazol se evaluo por el metodo de difusion en disco. El 88,8% de los aislados provenia de muestras de unas. C. parapsilosis fue la especie mas frecuente, seguida de C. tropicalis , C. albicans y C. krusei , lo cual confirmo el predominio de especies no albicans como causa de micosis superficial. El patron de susceptibilidad a flu...
Revista Iberoamericana De Micologia, 2013
Revista De La Sociedad Venezolana De Microbiologia, 2013
Resumen: El objetivo de este trabajo fue determinar la frecuencia de aislamiento de Candida spp. ... more Resumen: El objetivo de este trabajo fue determinar la frecuencia de aislamiento de Candida spp. en muestras clínicas de pacientes ingresados en una unidad de cuidados intensivos y evaluar su perfil de susceptibilidad in vitro a fluconazol y voriconazol. Se evaluaron 60 pacientes, procesándose 194 muestras: 98 hemocultivos, 48 urocultivos cuantitativos, 46 secreciones traqueales y 2 puntas de catéter, mediante siembra en agar cromogénico para Candida. El género y especie fue confirmado mediante las características macroscópicas de crecimiento, producción de clamidoconidias, hidrólisis de la urea y asimilación de carbohidratos. Para la evaluación de la susceptibilidad antifúngica se utilizó el método de difusión en disco M44-A del Clinical and Laboratory Standard Institute. Se aislaron 37 levaduras (73,0% de urocultivo en recuento significativo, 24,3% de secreción traqueal y 2,7% de punta de catéter). C. tropicalis fue la especie más frecuente (43,2%) seguida de C. glabrata (21,6%) y C. albicans (13,5%), evidenciándose una distribución diferente a la reportada en la literatura. El 73% de los aislados fue sensible a fluconazol y el 89,2% al voriconazol. La frecuencia observada de candiduria, sugiere que el urocultivo cuantitativo pudiera emplearse como estudio rutinario en pacientes recluidos en UCI, a fin de establecer estrategias terapéuticas y profilácticas adecuadas.
Revista De La Sociedad Venezolana De Microbiologia Num 1 Vol 34, 2015
Artículo original Aislamiento de Candida spp. en ambiente y personal que labora en una unidad de ... more Artículo original Aislamiento de Candida spp. en ambiente y personal que labora en una unidad de cuidados intensivos
Investigación clínica, 2015
Candida species other than C. albicans are often described as causative agents of superficial myc... more Candida species other than C. albicans are often described as causative agents of superficial mycosis and are more resistant to treatment with azoles. In order to determine the distribution of species and in vitro antifungal susceptibility of Candida spp., one ambispective study, which analyzed 18 yeast isolates obtained from samples from patients diagnosed with superficial mycosis, was performed. Taxonomic identification was performed by macroscopic visualization of the growth characteristics in chromogenic agar and by conventional methods. The susceptibility to fluconazole and voriconazole was evaluated by the disc diffusion method. Most of the isolates (88.8%), came from nail samples. C. parapsilosis was the most common species, followed by C. tropicalis, C. albicans and C. krusei, which confirmed the prevalence of non-albicans species as a cause of superficial mycoses. The pattern of susceptibility to fluconazole and voriconazole was similar: all isolates of C. parapsilosis and ...
Revista Iberoamericana de Micología, 2013
Antecedentes: En 1984 se crearon los Grupos de Trabajo en Micología de Venezuela (GTMV), proporci... more Antecedentes: En 1984 se crearon los Grupos de Trabajo en Micología de Venezuela (GTMV), proporcionando un abordaje novedoso al estudio de las micosis, en especial las micosis endémicas. Objetivos: Conocer los aportes en el estudio sistemático de las micosis en Venezuela durante 26 años de labor de los GTMV. Métodos: Se realizó revisión de la casuística publicada por los GTMV en el Boletín Informativo Las Micosis en Venezuela desde 1984 hasta 2010. Resultados: Se obtuvieron 36.968 diagnósticos de micosis superficiales, 1.989 de profundas sistémicas y 822 de profundas localizadas. La dermatofitosis fue la patología superficial más frecuente, paracoccidioidomicosis e histoplasmosis las profundas sistémicas, y cromoblastomicosis la profunda localizada. Se realizó la distribución geográfica de los casos de micosis profundas, pudiendo delimitar las zonas endémicas. Discusión: Las micosis superficiales constituyen un problema de salud pública por su alta morbilidad y pueden ser responsables de epidemias en grupos de riesgo. La paracoccidioidomicosis y la histoplamosis se reportaron con mayor frecuencia, incluso antes de haberse conformado los GTMV. El número de cromoblastomicosis y esporotricosis en Venezuela supera a lo reportado en otros países. Los GTMV han contribuido al conocimiento de la incidencia y prevalencia las micosis en el país, además de su divulgación como problema de salud pública, siendo un aporte invaluable que debe mantenerse en el tiempo, tratando no solo de reportar la casuística anual, sino también detallar aspectos clínico-epidemiológicos que permitan realizar seguimiento de la evolución de estas patologías.
Rev. Soc. Ven. …, 2011
... Leyla Humbría García a, *, Rosaura Hernández Valles a , Maigualida Pérez Blanco a , Leila Gar... more ... Leyla Humbría García a, *, Rosaura Hernández Valles a , Maigualida Pérez Blanco a , Leila García Hernández a , Mireya Mendoza b , Edgar ... Mondragón-González R, Méndez-Tovar LJ, Bernal-Vázquez E, Hernández-Hernández F, López-Martínez R, Manzano-Gayosso P, y col ...
Rev. Soc. Ven. Microbiol, 2010
cultivaron muestras de suelo para identificación de los aislados sugestivos de Coccidioides spp.,... more cultivaron muestras de suelo para identificación de los aislados sugestivos de Coccidioides spp., mediante examen directo de las muestras de tejido de ratones inoculados con el hongo. Las características ecoambientales del municipio y el análisis químico del suelo favorecen la presencia del hongo en esta zona. Del cultivo de suelos se obtuvo un aislado sugestivo de Coccidioides spp., pero el estudio en animales
Revista de la …, 2011
Resumen: La infección por el Virus de Hepatitis B (VHB) representa un problema de salud pública a... more Resumen: La infección por el Virus de Hepatitis B (VHB) representa un problema de salud pública a escala mundial, estimándose que más de dos millardos de personas en el mundo se han infectado, de los cuales aproximadamente 350 millones son portadores crónicos del virus. Cerca de un millón de muertes al año están relacionadas con hepatocarcinoma primario asociado a la infección por VHB, lo que hace a éste virus la cuarta causa de muerte por enfermedades infecciosas a nivel mundial. La replicación del VHB posee un potencial de variabilidad genética mayor que la de los virus ADN en general favoreciendo la aparición de mutantes naturales generadas por sustituciones puntuales, por reordenamiento de genes, o por cambios en los marcos de lectura denominados genotipos. La variabilidad genética podría estar asociada con las diferentes vías de transmisión, resistencia al interferón o progresión hacia el desarrollo de carcinoma hepatocelular.
Investigación clínica, 1996
This is a report of three cases of mycetome studied in our laboratory during the last four years.... more This is a report of three cases of mycetome studied in our laboratory during the last four years. We also informed about the evolution of the first case produced by Pyrenochaeta romeroi nova species reported by Borelli in 1959. All the patients were farmer men, 18, 42 and 54 years old, from the semiarid and subhumid zones of Falcon State, located on the northwest region of Venezuela. Clinically, all patients presented the mycetome syndrome (subcutaneous edema, sinus tract and "granules"), with a evolution of six months to three years. The lesions were localized in the inferior limbs in two of the patients and in the thorax in one of them. The clinical diagnose was confirmed by microbiological and mycological studies, isolating Nocardia brasiliensis in two cases and Madurella grisea in one. Two patients treated with Sulfas showed remission of the disease in a two years follow up. Including this report, 15 cases of mycetome have been reported in the Falcon State (Venezuela) ...