Roxana Carbó Zabala - Academia.edu (original) (raw)

Papers by Roxana Carbó Zabala

International Journal of Molecular Sciences, Mar 23, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Diabetes Research and Clinical Practice, Sep 1, 2000

Chaos, Solitons & Fractals

Journal of Biosciences, Feb 7, 2019

Among the last consequences of metabolic syndrome are cardiovascular complications such as infarc... more Among the last consequences of metabolic syndrome are cardiovascular complications such as infarcts. The hypoxic heart switches its lipid-based metabolism to carbohydrates, and a glucose-insulin-potassium (GIK) solution can be the metabolic support to protect the organ. Due to the physiology and cardiac risks associated with the metabolic syndrome, we studied the effect of GIK solution during hypoxia in a metabolic syndrome model by observing the participation of glucose transporters (GLUTs). The metabolic syndrome characteristics were established by giving a 30% sucrose drinking solution to Wistar rats for 24 weeks. The GIK solution's effect on myocyte glucose uptake during hypoxia and oxygenation was observed using a colorimetric method, and Western blot technique visualized the GLUT participation. Oxygenated control myocytes consumed 1.7 ± 0.2 µg of glucose per gram of fresh tissue per hour using the GLUT1, and during hypoxia, they incorporated 41.1% more glucose by GLUT1 and GLUT4. The GIK solution improved glucose uptake in oxygenation by 70.5% through GLUT1. In hypoxia, the uptake was 21% more than the hypoxic control group and by both GLUTs too. Oxygenated metabolic syndrome myocytes uptake was similar to control cells but achieved by both carriers in oxygenation and hypoxia. Also, the GIK solution had a better response in both oxygenation (113%) and hypoxia (71%). Despite the metabolic energy disorders of this syndrome, the GIK solution protects cardiomyocytes, in conditions of hypoxia, through the modulation of both GLUTs. So, this solution can be considered a useful resource during a heart attack in cases of metabolic syndrome.

Additional file 1: Table S1. Parasitemia curve from Ninoa T. cruzi strain by Pizzi–Brener method.

Nutricion hospitalaria, 2015

obesity is a major public health problem worldwide. The quantity and site of accumulation of adip... more obesity is a major public health problem worldwide. The quantity and site of accumulation of adipose tissue is of great importance for the physiopathology of this disease. the aim of this study was to assess the effect of a high carbohydrate diet on adipose tissue distribution. male Wistar rats, control (CONT) and high sucrose diet (HSD; 30% sucrose in their drinking water), were monitored during 24 weeks and total energy and macronutrient intake were estimated by measuring daily average consumption. A bioelectrical impedance procedure was performed at 22 weeks of treatment to assess body compartments and systolic arterial blood pressure was measured. Serum was obtained and retroperitoneal adipose tissue was collected and weighed. HSD ingested less pellets and beverage, consuming less lipids and proteins than CONT, but the same amount of carbohydrates. Retroperitoneal adipose tissue was more abundant in HSD. Both groups were normoglycemic; triglycerides, adiponectin and leptin level...

Cardiovascular Drugs and Therapy, 2015

The pathogenic mechanisms leading to cardiovascular disorders in patients with chronic kidney dis... more The pathogenic mechanisms leading to cardiovascular disorders in patients with chronic kidney disease have not been clearly established, although increased oxidative stress has been pointed out as a potential cause. Therefore, as cardiovascular events are still the first cause of death in patients with chronic kidney disease and traditional drugs or therapies rarely have effects on cardiac complications, we sought to determine the effect of curcumin in treating cardiac dysfunction in rats with established chronic renal disease. Treatment consisted in daily administration of curcumin (120 mg/kg/day) dissolved in 0.05 % carboxymethylcellulose via oral gavages during 30 days, beginning from day 30 after 5/6 nephrectomy (5/6Nx). Cardiac function, markers of oxidative stress, activation of PI3K/Akt/GSK3β and MEK1/2-ERK1/2 pathway, metalloproteinase-II (MMP-2) content, overall gelatinolytic activity, ROS production and mitochondrial integrity were evaluated after 1-month treatment. Curcumin restored systolic blood pressure, diminished interventricular and rear wall thickening, decreased left ventricle dimension at end-systole (LVSd) and restored ejection fraction in nephrectomized rats. Also, it diminished metalloproteinase-II levels and overall gelatinase activity, decreased oxidative stress and inhibited the mitochondrial permeability transition pore opening. Our findings suggest that curcumin might have therapeutic potential in treatment of heart disease in patients with established CKD by attenuating oxidative stress-related events as cardiac remodeling, mitochondrial dysfunction and cell death.

Metabolic syndrome (MetS) is characterized by a constellation of conditions that increase the ris... more Metabolic syndrome (MetS) is characterized by a constellation of conditions that increase the risk for heart disease and other health problems. Although multiple organs are affected by this syndrome, research that focuses on the organs that are not directly involved in metabolism is lacking. Because MetS increases the risk for various lung diseases, we explored the effects of MetS on the lung in this study. We observed that the chronic consumption of a high sucrose diet for 24 weeks induced MetS in a rat model. Concomitant expression changes in 1,026 coding genes and two microRNAs were observed in the analyzed lung tissues. A network analysis showed degradation changes in the melatonin pathway, which plays a protective role in the lung; impairments of several inflammatory pathways (i.e., interferon-γ and nuclear factor kappa B) were also revealed. Moreover, multiple olfactory genes were unexpectedly deregulated. Overall, the lungs of MetS mice exhibited an inflammatory phenotype wit...

SUMMARY Glucose homeostasis is seriously altered during stress periods such as sepsis, trauma and... more SUMMARY Glucose homeostasis is seriously altered during stress periods such as sepsis, trauma and surgery. Blood glucose levels rise up in critically ill patients or during the perioperative period, without distinction between normal or diabetic patients. This condition has been called stress hyperglycemia, and for many years it has been considered an adaptive and beneficial response from the organism. How- ever, new evidences have emerged showing that this condition increases mor- bidity and mortality in patients undergoing cardiac surgery and specially in those patients with extracorporeal circulation. In this review, it was analyzed the results of different studies of association between stress hyperglycemia and adverse outcomes of critically ill patients, those patients exposed to extensive surgery, especially cardiac surgery, as well as trauma patients. Here, the benefits of strict glycemic control (glucose levels between 80-110 mg/dL), using intensive insulin therapy in this g...

Archivos del Instituto de Cardiología de México

secciones de este sitio: Índice de este número Más revistas Búsqueda Others sections in this web ... more secciones de este sitio: Índice de este número Más revistas Búsqueda Others sections in this web site: Contents of this number More journals Search

In this chapter the authors will discuss some characteristics of the AA metabolism, generally ass... more In this chapter the authors will discuss some characteristics of the AA metabolism, generally associated with cardiovascular pathologies and particularly with MS. Also, details of the non-sexual effects of sex hormones and therapeutic agents, associated with the pathology, are included.

Baillière's Clinical Obstetrics and Gynaecology, 1996

Summary Combining the wealth of epidemiological, metabolic and recent mechanistic data, it would ... more Summary Combining the wealth of epidemiological, metabolic and recent mechanistic data, it would appear biologically plausible that HRT, either oestrogen alone or in combination with progestogen, is cardioprotective. Further research is required, as information is lacking on cardiovascular effects of HRT instigated at an older age. There is a need to identify cardiovascular benefit, indirect and/or direct, of combined oestrogen/progestogen therapy using randomized trials. The various progestogen types and doses also need to be investigated. Studies are also required to investigate the effect of HRT use in higher risk patients with established CVD. There is scant information on the effect of HRT on blood pressure of patients with hypertension. Cardiovascular risk factor profiles and incidence surveys need to be conducted in developing countries to characterize their female population and to identify the prevalence of CVD; this needs to be undertaken before widespread recommendations on CVD prevention and the role of HRT can be made. If HRT is to be used effectively in the future treatment of heart disease in women these questions need to be addressed. At present HRT is indicated for the relief of menopausal symptoms and the prevention of osteoporosis. In women without these indications, ORT may be recommended in those who have had a premature menopause, and possibly in those who have established CHD or who are at high risk of developing CHD. It is too early to suggest a blanket recommendation for the use of HRT in the treatment of the symptoms of women with established CVD, but HRT after the menopause may at least be safely used in the secondary prevention of CHD.

Synapse, 1997

The pattern of association between neurotensin (NT)-immunoreactive (NTIR) preganglionic nerve ter... more The pattern of association between neurotensin (NT)-immunoreactive (NTIR) preganglionic nerve terminals and cardiac and noncardiac neurons in the stellate ganglion of the cat is analyzed, based on the finding of an excitatory modulation effect of exogenous NT on cardiac functions. For this purpose, NT-containing terminals were labeled by immunohistochemistry, and ganglion cells were detected by retrograde labeling of cardiac and vertebral nerves to identify cardiac and noncardiac neurons. To determine a possible regional localization of NTIR terminals and ganglion cells, the ganglia were divided into four areas: caudal, dorsomedial, cranial, and ventromedial, related to the two major afferent nerves (thoracic white rami 3 [T3WR] and 2 [T2WR]) and the two efferent nerves (vertebral and cardiac). NTIR terminals were widespread in the complete ganglion tissue; they covered practically all the regions explored, although two clusters of high concentration of NTIR terminals were detected in the cranial and caudal areas. By retrograde labelling it was found that cardiac cells were arranged around the exit of the cardiac nerve and that the vertebral neurons were extended from the exit of the vertebral nerve to the entrance of T3WR. The finding of association of NTIR terminals with cardiac neurons may account for the cardioregulatory effect of NT; however, since the presence of NTIR terminals close to the noncardiac neurons is notorious, other regulatory functions of NT must be considered.

Heart and Vessels, 2002

We studied whether the contractile responses to potassium chloride (KCl) of rat coronary and femo... more We studied whether the contractile responses to potassium chloride (KCl) of rat coronary and femoral arteries differ when perfused with solutions containing varying concentrations of metabolic substrates (glucose, oxygen, insulin) and whether these differences might explain some of the beneficial effects of glucose-insulin-potassium (GIK or polarizing) solution. We also studied the mediation of the endothelium on these effects. Contraction of femoral arteries decreased with low and high glucose while in coronary arteries it increased as the glucose concentrations rose. Endothelin receptor antagonists blocked this increase. The tension increase induced by insulin in both vessels was smaller in the coronary arteries. The ET A receptor antagonist PD51242 blocked most of the insulin effect in the coronary arteries, whereas in the femoral arteries this was blocked by the ET B receptor antagonist BQ788. Hypoxia blocked the constrictive responses to glucose and insulin of the coronary but not of the femoral arteries. GIK increased contractile force in hypoxic femoral arteries but reduced that in coronary arteries. The contractile response decreased by GIK in hypoxic coronary arteries was restored by N G-nitro-l-arginine methyl ester, suggesting mediation by nitric oxide. We conclude that in the presence of high glucose and/or insulin levels, coronary arteries respond to KCl differently from femoral arteries; their behavior during hypoxia might explain some of the beneficial effects of GIK.

European Journal of Pharmacology, 2007

Archivos de Cardiología de México, 2015

Resumen La omentina es una nueva adipocina a la que se le ha atribuido la capacidad de regular ac... more Resumen La omentina es una nueva adipocina a la que se le ha atribuido la capacidad de regular actividades metabólicas (sensibilidad a la insulina) y antiinflamatorias, ofreciendo protección cardiovascular en la obesidad y diabetes mellitus tipo 2. Por lo anterior, es importante conocer los mecanismos a través de los cuales confiere protección cardiovascular, con el objetivo de considerar la omentina como blanco o agente terapéutico en este escenario.