Ruchika Ghanekar - Academia.edu (original) (raw)

Papers by Ruchika Ghanekar

We report a cost effective and eco-friendly biosynthesis of gold nanoparticles (F-AuNPs) using aq... more We report a cost effective and eco-friendly biosynthesis of gold nanoparticles (F-AuNPs) using aqueous extract of Ficus religiosa as the reducing and stabilizing agent. These nanoparticles were characterized by various techniques such as UV-Vis, XRD, TEM and FTIR. The characteristic surface plasmon peak was observed at 540 nm while XRD analysis suggested it to be a face-centered cubic (fcc) structure with peaks at 38.06, 44.46, 64.75 and 77.56. FTIR studies indicated the capping of the nanoparticles with polyphenols, amines and carboxylates present in the extract of Ficus religiosa whereas TEM analysis showed spherical morphology with other shapes such as triangles and hexagons. The F-AuNPs were found to be non-toxic to HEK 293 cells, thereby suggesting their potential application in the field of nanobiotechnology.

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: In the present study, we have performed the acute and sub-acute toxicity of a standard... more Objective: In the present study, we have performed the acute and sub-acute toxicity of a standardized polyherbal formulation (HC9) in Swiss albino mice. Methods: In acute toxicity study, the mice were orally administered with different doses (1750 and 2000 mg/kg) of HC9 and monitored for 14 d. In the sub-acute toxicity study, animals received HC9 extract by oral gavage at the doses of 250, 500 and 1000 mg/kg/day (𝑛=5/group/sex) for 28 d. At the end of the study, the animals were sacrificed and evaluated for effect of HC9 on biochemical, hematological and histopathological parameters. Results: HC9 did not produce any adverse effects in biochemical, hematological, urine and histopathological parameters in mice. HC9 did not induce any adverse effects in terms of mortality and clinical signs in an acute toxicity study. It was well-tolerated by mice up to 2000 mg/kg/body weight. In sub-acute toxicity study, no treatment-related adverse effects were found in the mice upto 1000 mg/kg/day d...

Journal of Ethnopharmacology, 2021

ETHNOPHARMACOLOGICAL RELEVANCE Panchvalkala, an Ayurvedic traditional formulation has references ... more ETHNOPHARMACOLOGICAL RELEVANCE Panchvalkala, an Ayurvedic traditional formulation has references in Charak Samhita and Bhavaprakasha Nighantu for the treatment of women with endometriosis-related problems, leucorrhea and vaginal ailments. The formulation comprises of equal ratios of barks from Ficus glomerata, Ficus virens, Ficus religiosa, Ficus benghalensis, and Thespesia populnea. AIM OF THE STUDY The present study aimed to evaluate the anticancer and immunomodulatory activity of aqueous extract of Panchvalkala (PVaq) against cervical cancer in vitro and in vivo. MATERIALS AND METHODS The effect of PVaq on disruption of mitochondrial membrane potential in cervical cancer cervical cancer cell lines, SiHa and HeLa, was studied by using JC1 dye. The expression of generic caspases in the cells after treatment with PVaq was evaluated by ELISA kit. The expression of pRb, p53, E6 and E7 proteins were evaluated by western blotting. Acute oral toxicity and DRF studies were performed in Swiss albino mice by following OECD guidelines 423 and 407, respectively. Tumor retardation study was done in C57BL/6 mouse papilloma model. The mice were divided into six groups: No tumor control (NTC), Tumor control (TC), Cisplatin (Cis) (4 mg/kg b.w.), PVaq 100, 200 mg/kg b.w and combination of PVaq (200 mg/kg b.w.) and Cisplatin (4 mg/kg b.w.).The mice were orally gavaged with PVaq daily for 14 days and cisplatin was given intravenously on every 1st, 5th and 9th day. Hematological and biochemical parameters were studied by using hematology analyzer and kits, respectively. E6 and E7 gene expression in the tumor samples was determined by qPCR. Th1 and Th2 cytokine levels were determined by ELISA. RESULTS PVaq induced mitochondrial depolarization in SiHa and HeLa, and increased the expression of generic caspases, resulting into apoptosis. PVaq upregulated the expression of tumor suppressor proteins (p53 and pRb) and reduced the expression of viral oncoproteins (E6 and E7). Acute toxicity study displayed non-toxicity of PVaq while DRF study ensured its safe dose for further efficacy studies. PVaq reduced tumor volume and weight in mouse papilloma model and induced immunomodulation in the animals. It increased serum levels of IL-2 (Th1) with a concomitant decrease in IL-10 (Th2) cytokines. The drug did not affect body weight, food consumption and organ histopathology of the animals. CONCLUSIONS PVaq exhibited anticancer and immunomodulatory activities against cervical cancer cells and female mouse papilloma model.

Background: Triphala, an Ayurvedic polyherbal formulation, is used for the treatment of various d... more Background: Triphala, an Ayurvedic polyherbal formulation, is used for the treatment of various diseases including obesity. Objective: The present study was planned to evaluate the anti-adipogenic potential of aqueous extract of Triphala (TPaq) using 3T3-L1 adipocyte cell line model. Methods: The effect of aqueous extract of Triphala (TPaq) was tested on the viability of 3T3- L1 cells by MTT assay. The cells were treated with a cocktail of dexamethasone (DEX), isobutylmethylxanthine (IBMX) and insulin to induce adipogenesis. The cells were treated either with the induction cocktail or with the cocktail containing different concentrations (1, 10 and100 μg/ml) of TPaq. Intracellular lipid content was analyzed using Oil O Red stain and was quantified after extracting with isopropanol at 500 nm wavelength. The expression of early (PPAR-γ and C/EBP-α) and late (GLUT4 and FAS) phase adipogenic genes was studied by real time PCR. Results: TPaqdid not affect the viability of 3T3-L1 cell lin...

Prostaglandins & Other Lipid Mediators, 2019

Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncopr... more Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer,

Prostaglandins & Other Lipid Mediators, 2019

Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncopr... more Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer,

Omega 3 fatty acids (n3 PUFAs) have been shown to exert suppressive activity on cancer cell proli... more Omega 3 fatty acids (n3 PUFAs) have been shown to exert suppressive activity on cancer cell proliferation as compared to omega 6 PUFAs. Various In vitro, In vivo and clinical studies have reiterated that n6 PUFAs are pro-cancerous compared to n3 PUFAs that are anticancerous. Considering these experimental findings, incorporation of omega 3 fatty acid rich diet in our daily routine may be responsible to suppress the final steps of carcinogenesis such as angiogenesis and metastasis. This review focuses on the significance of omega 3 fatty acids in the prevention of cancer and thereby signifies their role in human diet.

In the present work, we have analyzed the effect of the essential fatty acid, alpha linolenic aci... more In the present work, we have analyzed the effect of the essential fatty acid, alpha linolenic acid (ALA) on nitric oxide release as well as induction of lipid peroxidation in breast (MCF-7 and MDA-MB-231) and cervical (SiHa and HeLa) cancer cell lines. ALA-treated cells showed a dose-dependent decrease in cell viability in both breast and cervical cancer cell lines without affecting the viability of non-cancerous transformed HEK 293 cells. Both types of cancer cells treated with ALA demonstrated a significant reduction in nitric oxide (NO) release with a simultaneous increase in lipid peroxidation (LPO). This was followed by a decrease in the mitochondrial membrane potential as well as activation of caspase 3 leading to apoptosis. Thus, ALA regulated the growth of cancer cell lines through induction of lipid peroxidation and modulation of nitric oxide release resulting in apoptosis

BMC Cancer, 2010

Background Chemoprevention, which includes the use of synthetic or natural agents (alone or in co... more Background Chemoprevention, which includes the use of synthetic or natural agents (alone or in combination) to block the development of cancer in human beings, is an extremely promising strategy for cancer prevention. Cinnamon is one of the most widely used herbal medicines with diverse biological activities including anti-tumor activity. In the present study, we have reported the anti-neoplastic activity of cinnamon in cervical cancer cell line, SiHa. Methods The aqueous cinnamon extract (ACE-c) was analyzed for its cinnamaldehyde content by HPTLC analysis. The polyphenol content of ACE-c was measured by Folin-Ciocalteau method. Cytotoxicity analysis was performed by MTT assay. We studied the effect of cinnamon on growth kinetics by performing growth curve, colony formation and soft agar assays. The cells treated with ACE-c were analyzed for wound healing assay as well as for matrix metalloproteinase-2 (MMP-2) expression at mRNA and protein level by RT-PCR and zymography, respectiv...

Asian Pacific Journal of Cancer Care, 2021

Background: Breast cancer is the leading cancer diagnosed in Indian women. Lifestyle related fact... more Background: Breast cancer is the leading cancer diagnosed in Indian women. Lifestyle related factors such as high body mass index (BMI) and obesity have been recognized as major risk factors for the development of breast cancer. However, India has higher proportion of underweight population and recently positive correlation has been reported between underweight and increased risk of breast cancer. We have attempted to study an association between low BMI and total body fat percentage with breast cancer risk by performing retrospective analysis on a small sample size of 41 female patients diagnosed with breast cancer. The data was collected from Department of Oncology, Bharati Vidyapeeth Hospital and Research Centre (BVHRC), Pune, India. Methods: Binary logistic regression was performed to estimate odds ratios (ORs) and to examine the predictive effect of each factor on the breast cancer risk. Results: It was observed that underweight population displayed higher risk of breast cancer...

Background: HC9, a polyherbal formulation, is based on Stanyashodhana Kashaya (an Ayurvedic formu... more Background: HC9, a polyherbal formulation, is based on Stanyashodhana Kashaya (an Ayurvedic formulation) that is being prescribed by Ayurvedic physicians for the treatment of various disorders of mammary glands. We have recently reported anticancer activity of HC9 in breast cancer cell lines through various molecular mechanisms. Few studies have shown an association between breast cancer and melanoma that has prompted us to find whether HC9 could regulate the melanoma growth as well. Aim of the Study: The aim was to investigate the tumor retardation and immunomodulatory potential of HC9 in mouse melanoma model. Materials and Methods: C57BL/6 mice, with B16F10-induced melanoma tumors, were divided into six groups: tumor control, doxorubicin (2 mg/kg body weight [b.w.]), low dose (100 mg/kg b.w.), intermediate (200 mg/kg b.w.), and high dose (400 mg/kg b.w.) of HC9. No tumor control served as the negative control group. The mice were orally gavaged with HC9 daily for 3 weeks. The urin...

Scaffold/matrix-associated region-1-binding protein (SMAR1) specifically interacts with the MARbe... more Scaffold/matrix-associated region-1-binding protein (SMAR1) specifically interacts with the MARbeta sequence, which is located 400-bp upstream of the murine TCRbeta enhancer and is highly expressed during the DP stage of thymocyte development. To further analyze the functions of SMAR1, transgenic mice were generated that express SMAR1 in a tissue-independent manner. SMAR1-overexpressing mice exhibit severely altered frequency of the T cells expressing commonly used Vbetas (Vbeta5.1/5.2 and Vbeta8.1/8.2/8.3). The rearrangements of Vbeta5.1/5.2, Vbeta8.1/8.2/8.3 loci are also reduced in SMAR1 transgenic mice. The T cells in SMAR1 transgenic mice exhibit a mild perturbation at the early DN stage. SMAR1 transgenic mice exhibit hypercellular lymph nodes and spleen accompanied with prominent architectural defects in these organs. These results indicate that SMAR1 plays an important role in the regulation of T cell development as well as V(D)J recombination besides maintaining the architec...

Objective: Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to have antican... more Objective: Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to have anticancer activity. We investigated the anti-cancer activity of cetyl alcohol sophorolipids (CAS) and oleic acid sophorolipids (OAS) in breast cancer (MCF-7, MDA-MB-231), cervical cancer (SiHa, HeLa) and non-cancerous (HaCaT and RAW264.7) cell lines. Methods: For cell viability assay, MCF-7, MDA-MB-231, SiHa, HeLa, HaCaT and RAW264.7 cell lines were treated with different concentrations (0-160 µg/ml) of OAS and CAS for 24h. The cell viability was determined by MTT dye uptake method. Cell proliferation assay was determined by using trypan blue dye exclusion method. Results: Our preliminary data shows that compared to OAS, CAS exhibited more significant reduction in the viability of MCF-7, MDA-MB-231 and SiHa. However, compared to CAS, OAS induced more decrease in viability in HeLa cells. Interestingly, both the types of SLs did not affect the viability of non-cancerous cells. Moreover, CAS, whe...

Histone deacetylase 8 (HDAC8) has emerged as a promising drug target for cancer therapeutics deve... more Histone deacetylase 8 (HDAC8) has emerged as a promising drug target for cancer therapeutics development. HDAC8 has been reported to regulate cancer cell proliferation, invasion and promote metastasis through modulation of cell cycle associated proteins. Of late, phytocompounds have been demonstrated to exhibit anticancer and anti-HDAC8 activity. Here, we have shown the HDAC8 inhibitory potential of an active phytocompound from HC9 (herbal composition-9), a polyherbal anticancer formulation based on the traditional Ayurvedic drug, Stanya Shodhan Kashaya. HC9 was recently reported to exhibit anticancer activity against breast cancer cells through induction of cell cycle arrest, decrease in migration and invasion as well as regulation of inflammation and chromatin modulators. In silico studies such as molecular docking, molecular dynamics (MD) simulation and binding free energy analyses showed greater binding energy values and interaction stability of MA with HDAC8 compared to other p...

Pharmacognosy Magazine

Background: Ocimum sanctum (OSE) has been used in the Indian system of traditional medicine (Ayur... more Background: Ocimum sanctum (OSE) has been used in the Indian system of traditional medicine (Ayurveda) for the treatment and prevention of various diseases. However, the contradictory reports regarding the reproductive safety of OSE prompted us to re-verify its effect on the reproductive system by following internationally accepted Organization for Economic Cooperation and Development guideline 415. Objective: To study the effect of aqueous/methanolic extract of OSE (OciBest) on the male and female reproductive performance of Wistar rats. Materials and Methods: Rats were orally gavaged with OciBest at dose levels of 0, 250, 500, and 1000 mg/kg. Males were administered with OciBest for 12 weeks before and during the mating period. On the other hand, females received OciBest for 2 weeks before mating until the end of the lactation period. Results: OciBest at 1000 mg/kg did not induce any adverse effects on the reproductive performance of male and female rats. All the treated parent animals survived until the end of the study period with no major signs of clinical toxicity. The body weights, food consumption, male and female fertility indices, organ weights, as well as gross pathological and histopathology observations in parent animals, did not reveal any treatment-related adverse effects. Moreover, in comparison to the control groups, OciBest did not induce any treatment-related adverse effects to the offsprings. Conclusion: Thus, no-observed adverse effect level was found up to 1000 mg/kg dose, suggesting the safety of OciBest for reproductive system. Abbreviations used: OECD: Organization for Economic Cooperation and Development; NOAEL: No-observed adverse effect level; CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals; OA: Oleanolic acid; UA: Ursolic acid.

Minimal and Noninvasive Early Diagnosis and Prognosis, 2013

New Journal of Chemistry

The bifunctional nature of nanoceria as pro-drug and vehicle for the site-specific targeted deliv... more The bifunctional nature of nanoceria as pro-drug and vehicle for the site-specific targeted delivery of DOX is achieved with CeO2–(DOX–FA) nanoconjugates towards MFC-7 cells.

New Journal of Chemistry

FiCF NPs induced apoptosis in breast cancer cells, exhibited safety, reduced tumor burden in mice... more FiCF NPs induced apoptosis in breast cancer cells, exhibited safety, reduced tumor burden in mice due to increased pharmacological efficacy.

Journal of Biological Chemistry

This article has been withdrawn by the authors. In Fig 2A, a portion of the image for 24 hrs Cont... more This article has been withdrawn by the authors. In Fig 2A, a portion of the image for 24 hrs Control was reused in hrs SMAR1 stable. The Mdm2 immunoblot in Fig. 5E was mislabeled in Fig. 5F. In Fig. 9E, image 3 was flipped vertically and reused as image 1. Also, a portion of image 5 was resized and reused in image 7. The H&E staining in Fig. 10F was rotated in Fig. 10E. The authors state that they could not trace a few of the original images, so they repeated all the relevant experiments and they state that the results are the same as the published data. The authors thus stand by the overall conclusion of the study.

New Journal of Chemistry

A rapid, sensitive and accessible HPLC-UV method was developed and validated for simultaneous det... more A rapid, sensitive and accessible HPLC-UV method was developed and validated for simultaneous detection of CNAD and CA up to 1.0 ng ml−1 in plasma samples.

We report a cost effective and eco-friendly biosynthesis of gold nanoparticles (F-AuNPs) using aq... more We report a cost effective and eco-friendly biosynthesis of gold nanoparticles (F-AuNPs) using aqueous extract of Ficus religiosa as the reducing and stabilizing agent. These nanoparticles were characterized by various techniques such as UV-Vis, XRD, TEM and FTIR. The characteristic surface plasmon peak was observed at 540 nm while XRD analysis suggested it to be a face-centered cubic (fcc) structure with peaks at 38.06, 44.46, 64.75 and 77.56. FTIR studies indicated the capping of the nanoparticles with polyphenols, amines and carboxylates present in the extract of Ficus religiosa whereas TEM analysis showed spherical morphology with other shapes such as triangles and hexagons. The F-AuNPs were found to be non-toxic to HEK 293 cells, thereby suggesting their potential application in the field of nanobiotechnology.

International Journal of Pharmacy and Pharmaceutical Sciences, 2015

Objective: In the present study, we have performed the acute and sub-acute toxicity of a standard... more Objective: In the present study, we have performed the acute and sub-acute toxicity of a standardized polyherbal formulation (HC9) in Swiss albino mice. Methods: In acute toxicity study, the mice were orally administered with different doses (1750 and 2000 mg/kg) of HC9 and monitored for 14 d. In the sub-acute toxicity study, animals received HC9 extract by oral gavage at the doses of 250, 500 and 1000 mg/kg/day (𝑛=5/group/sex) for 28 d. At the end of the study, the animals were sacrificed and evaluated for effect of HC9 on biochemical, hematological and histopathological parameters. Results: HC9 did not produce any adverse effects in biochemical, hematological, urine and histopathological parameters in mice. HC9 did not induce any adverse effects in terms of mortality and clinical signs in an acute toxicity study. It was well-tolerated by mice up to 2000 mg/kg/body weight. In sub-acute toxicity study, no treatment-related adverse effects were found in the mice upto 1000 mg/kg/day d...

Journal of Ethnopharmacology, 2021

ETHNOPHARMACOLOGICAL RELEVANCE Panchvalkala, an Ayurvedic traditional formulation has references ... more ETHNOPHARMACOLOGICAL RELEVANCE Panchvalkala, an Ayurvedic traditional formulation has references in Charak Samhita and Bhavaprakasha Nighantu for the treatment of women with endometriosis-related problems, leucorrhea and vaginal ailments. The formulation comprises of equal ratios of barks from Ficus glomerata, Ficus virens, Ficus religiosa, Ficus benghalensis, and Thespesia populnea. AIM OF THE STUDY The present study aimed to evaluate the anticancer and immunomodulatory activity of aqueous extract of Panchvalkala (PVaq) against cervical cancer in vitro and in vivo. MATERIALS AND METHODS The effect of PVaq on disruption of mitochondrial membrane potential in cervical cancer cervical cancer cell lines, SiHa and HeLa, was studied by using JC1 dye. The expression of generic caspases in the cells after treatment with PVaq was evaluated by ELISA kit. The expression of pRb, p53, E6 and E7 proteins were evaluated by western blotting. Acute oral toxicity and DRF studies were performed in Swiss albino mice by following OECD guidelines 423 and 407, respectively. Tumor retardation study was done in C57BL/6 mouse papilloma model. The mice were divided into six groups: No tumor control (NTC), Tumor control (TC), Cisplatin (Cis) (4 mg/kg b.w.), PVaq 100, 200 mg/kg b.w and combination of PVaq (200 mg/kg b.w.) and Cisplatin (4 mg/kg b.w.).The mice were orally gavaged with PVaq daily for 14 days and cisplatin was given intravenously on every 1st, 5th and 9th day. Hematological and biochemical parameters were studied by using hematology analyzer and kits, respectively. E6 and E7 gene expression in the tumor samples was determined by qPCR. Th1 and Th2 cytokine levels were determined by ELISA. RESULTS PVaq induced mitochondrial depolarization in SiHa and HeLa, and increased the expression of generic caspases, resulting into apoptosis. PVaq upregulated the expression of tumor suppressor proteins (p53 and pRb) and reduced the expression of viral oncoproteins (E6 and E7). Acute toxicity study displayed non-toxicity of PVaq while DRF study ensured its safe dose for further efficacy studies. PVaq reduced tumor volume and weight in mouse papilloma model and induced immunomodulation in the animals. It increased serum levels of IL-2 (Th1) with a concomitant decrease in IL-10 (Th2) cytokines. The drug did not affect body weight, food consumption and organ histopathology of the animals. CONCLUSIONS PVaq exhibited anticancer and immunomodulatory activities against cervical cancer cells and female mouse papilloma model.

Background: Triphala, an Ayurvedic polyherbal formulation, is used for the treatment of various d... more Background: Triphala, an Ayurvedic polyherbal formulation, is used for the treatment of various diseases including obesity. Objective: The present study was planned to evaluate the anti-adipogenic potential of aqueous extract of Triphala (TPaq) using 3T3-L1 adipocyte cell line model. Methods: The effect of aqueous extract of Triphala (TPaq) was tested on the viability of 3T3- L1 cells by MTT assay. The cells were treated with a cocktail of dexamethasone (DEX), isobutylmethylxanthine (IBMX) and insulin to induce adipogenesis. The cells were treated either with the induction cocktail or with the cocktail containing different concentrations (1, 10 and100 μg/ml) of TPaq. Intracellular lipid content was analyzed using Oil O Red stain and was quantified after extracting with isopropanol at 500 nm wavelength. The expression of early (PPAR-γ and C/EBP-α) and late (GLUT4 and FAS) phase adipogenic genes was studied by real time PCR. Results: TPaqdid not affect the viability of 3T3-L1 cell lin...

Prostaglandins & Other Lipid Mediators, 2019

Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncopr... more Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer,

Prostaglandins & Other Lipid Mediators, 2019

Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncopr... more Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer,

Omega 3 fatty acids (n3 PUFAs) have been shown to exert suppressive activity on cancer cell proli... more Omega 3 fatty acids (n3 PUFAs) have been shown to exert suppressive activity on cancer cell proliferation as compared to omega 6 PUFAs. Various In vitro, In vivo and clinical studies have reiterated that n6 PUFAs are pro-cancerous compared to n3 PUFAs that are anticancerous. Considering these experimental findings, incorporation of omega 3 fatty acid rich diet in our daily routine may be responsible to suppress the final steps of carcinogenesis such as angiogenesis and metastasis. This review focuses on the significance of omega 3 fatty acids in the prevention of cancer and thereby signifies their role in human diet.

In the present work, we have analyzed the effect of the essential fatty acid, alpha linolenic aci... more In the present work, we have analyzed the effect of the essential fatty acid, alpha linolenic acid (ALA) on nitric oxide release as well as induction of lipid peroxidation in breast (MCF-7 and MDA-MB-231) and cervical (SiHa and HeLa) cancer cell lines. ALA-treated cells showed a dose-dependent decrease in cell viability in both breast and cervical cancer cell lines without affecting the viability of non-cancerous transformed HEK 293 cells. Both types of cancer cells treated with ALA demonstrated a significant reduction in nitric oxide (NO) release with a simultaneous increase in lipid peroxidation (LPO). This was followed by a decrease in the mitochondrial membrane potential as well as activation of caspase 3 leading to apoptosis. Thus, ALA regulated the growth of cancer cell lines through induction of lipid peroxidation and modulation of nitric oxide release resulting in apoptosis

BMC Cancer, 2010

Background Chemoprevention, which includes the use of synthetic or natural agents (alone or in co... more Background Chemoprevention, which includes the use of synthetic or natural agents (alone or in combination) to block the development of cancer in human beings, is an extremely promising strategy for cancer prevention. Cinnamon is one of the most widely used herbal medicines with diverse biological activities including anti-tumor activity. In the present study, we have reported the anti-neoplastic activity of cinnamon in cervical cancer cell line, SiHa. Methods The aqueous cinnamon extract (ACE-c) was analyzed for its cinnamaldehyde content by HPTLC analysis. The polyphenol content of ACE-c was measured by Folin-Ciocalteau method. Cytotoxicity analysis was performed by MTT assay. We studied the effect of cinnamon on growth kinetics by performing growth curve, colony formation and soft agar assays. The cells treated with ACE-c were analyzed for wound healing assay as well as for matrix metalloproteinase-2 (MMP-2) expression at mRNA and protein level by RT-PCR and zymography, respectiv...

Asian Pacific Journal of Cancer Care, 2021

Background: Breast cancer is the leading cancer diagnosed in Indian women. Lifestyle related fact... more Background: Breast cancer is the leading cancer diagnosed in Indian women. Lifestyle related factors such as high body mass index (BMI) and obesity have been recognized as major risk factors for the development of breast cancer. However, India has higher proportion of underweight population and recently positive correlation has been reported between underweight and increased risk of breast cancer. We have attempted to study an association between low BMI and total body fat percentage with breast cancer risk by performing retrospective analysis on a small sample size of 41 female patients diagnosed with breast cancer. The data was collected from Department of Oncology, Bharati Vidyapeeth Hospital and Research Centre (BVHRC), Pune, India. Methods: Binary logistic regression was performed to estimate odds ratios (ORs) and to examine the predictive effect of each factor on the breast cancer risk. Results: It was observed that underweight population displayed higher risk of breast cancer...

Background: HC9, a polyherbal formulation, is based on Stanyashodhana Kashaya (an Ayurvedic formu... more Background: HC9, a polyherbal formulation, is based on Stanyashodhana Kashaya (an Ayurvedic formulation) that is being prescribed by Ayurvedic physicians for the treatment of various disorders of mammary glands. We have recently reported anticancer activity of HC9 in breast cancer cell lines through various molecular mechanisms. Few studies have shown an association between breast cancer and melanoma that has prompted us to find whether HC9 could regulate the melanoma growth as well. Aim of the Study: The aim was to investigate the tumor retardation and immunomodulatory potential of HC9 in mouse melanoma model. Materials and Methods: C57BL/6 mice, with B16F10-induced melanoma tumors, were divided into six groups: tumor control, doxorubicin (2 mg/kg body weight [b.w.]), low dose (100 mg/kg b.w.), intermediate (200 mg/kg b.w.), and high dose (400 mg/kg b.w.) of HC9. No tumor control served as the negative control group. The mice were orally gavaged with HC9 daily for 3 weeks. The urin...

Scaffold/matrix-associated region-1-binding protein (SMAR1) specifically interacts with the MARbe... more Scaffold/matrix-associated region-1-binding protein (SMAR1) specifically interacts with the MARbeta sequence, which is located 400-bp upstream of the murine TCRbeta enhancer and is highly expressed during the DP stage of thymocyte development. To further analyze the functions of SMAR1, transgenic mice were generated that express SMAR1 in a tissue-independent manner. SMAR1-overexpressing mice exhibit severely altered frequency of the T cells expressing commonly used Vbetas (Vbeta5.1/5.2 and Vbeta8.1/8.2/8.3). The rearrangements of Vbeta5.1/5.2, Vbeta8.1/8.2/8.3 loci are also reduced in SMAR1 transgenic mice. The T cells in SMAR1 transgenic mice exhibit a mild perturbation at the early DN stage. SMAR1 transgenic mice exhibit hypercellular lymph nodes and spleen accompanied with prominent architectural defects in these organs. These results indicate that SMAR1 plays an important role in the regulation of T cell development as well as V(D)J recombination besides maintaining the architec...

Objective: Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to have antican... more Objective: Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to have anticancer activity. We investigated the anti-cancer activity of cetyl alcohol sophorolipids (CAS) and oleic acid sophorolipids (OAS) in breast cancer (MCF-7, MDA-MB-231), cervical cancer (SiHa, HeLa) and non-cancerous (HaCaT and RAW264.7) cell lines. Methods: For cell viability assay, MCF-7, MDA-MB-231, SiHa, HeLa, HaCaT and RAW264.7 cell lines were treated with different concentrations (0-160 µg/ml) of OAS and CAS for 24h. The cell viability was determined by MTT dye uptake method. Cell proliferation assay was determined by using trypan blue dye exclusion method. Results: Our preliminary data shows that compared to OAS, CAS exhibited more significant reduction in the viability of MCF-7, MDA-MB-231 and SiHa. However, compared to CAS, OAS induced more decrease in viability in HeLa cells. Interestingly, both the types of SLs did not affect the viability of non-cancerous cells. Moreover, CAS, whe...

Histone deacetylase 8 (HDAC8) has emerged as a promising drug target for cancer therapeutics deve... more Histone deacetylase 8 (HDAC8) has emerged as a promising drug target for cancer therapeutics development. HDAC8 has been reported to regulate cancer cell proliferation, invasion and promote metastasis through modulation of cell cycle associated proteins. Of late, phytocompounds have been demonstrated to exhibit anticancer and anti-HDAC8 activity. Here, we have shown the HDAC8 inhibitory potential of an active phytocompound from HC9 (herbal composition-9), a polyherbal anticancer formulation based on the traditional Ayurvedic drug, Stanya Shodhan Kashaya. HC9 was recently reported to exhibit anticancer activity against breast cancer cells through induction of cell cycle arrest, decrease in migration and invasion as well as regulation of inflammation and chromatin modulators. In silico studies such as molecular docking, molecular dynamics (MD) simulation and binding free energy analyses showed greater binding energy values and interaction stability of MA with HDAC8 compared to other p...

Pharmacognosy Magazine

Background: Ocimum sanctum (OSE) has been used in the Indian system of traditional medicine (Ayur... more Background: Ocimum sanctum (OSE) has been used in the Indian system of traditional medicine (Ayurveda) for the treatment and prevention of various diseases. However, the contradictory reports regarding the reproductive safety of OSE prompted us to re-verify its effect on the reproductive system by following internationally accepted Organization for Economic Cooperation and Development guideline 415. Objective: To study the effect of aqueous/methanolic extract of OSE (OciBest) on the male and female reproductive performance of Wistar rats. Materials and Methods: Rats were orally gavaged with OciBest at dose levels of 0, 250, 500, and 1000 mg/kg. Males were administered with OciBest for 12 weeks before and during the mating period. On the other hand, females received OciBest for 2 weeks before mating until the end of the lactation period. Results: OciBest at 1000 mg/kg did not induce any adverse effects on the reproductive performance of male and female rats. All the treated parent animals survived until the end of the study period with no major signs of clinical toxicity. The body weights, food consumption, male and female fertility indices, organ weights, as well as gross pathological and histopathology observations in parent animals, did not reveal any treatment-related adverse effects. Moreover, in comparison to the control groups, OciBest did not induce any treatment-related adverse effects to the offsprings. Conclusion: Thus, no-observed adverse effect level was found up to 1000 mg/kg dose, suggesting the safety of OciBest for reproductive system. Abbreviations used: OECD: Organization for Economic Cooperation and Development; NOAEL: No-observed adverse effect level; CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals; OA: Oleanolic acid; UA: Ursolic acid.

Minimal and Noninvasive Early Diagnosis and Prognosis, 2013

New Journal of Chemistry

The bifunctional nature of nanoceria as pro-drug and vehicle for the site-specific targeted deliv... more The bifunctional nature of nanoceria as pro-drug and vehicle for the site-specific targeted delivery of DOX is achieved with CeO2–(DOX–FA) nanoconjugates towards MFC-7 cells.

New Journal of Chemistry

FiCF NPs induced apoptosis in breast cancer cells, exhibited safety, reduced tumor burden in mice... more FiCF NPs induced apoptosis in breast cancer cells, exhibited safety, reduced tumor burden in mice due to increased pharmacological efficacy.

Journal of Biological Chemistry

This article has been withdrawn by the authors. In Fig 2A, a portion of the image for 24 hrs Cont... more This article has been withdrawn by the authors. In Fig 2A, a portion of the image for 24 hrs Control was reused in hrs SMAR1 stable. The Mdm2 immunoblot in Fig. 5E was mislabeled in Fig. 5F. In Fig. 9E, image 3 was flipped vertically and reused as image 1. Also, a portion of image 5 was resized and reused in image 7. The H&E staining in Fig. 10F was rotated in Fig. 10E. The authors state that they could not trace a few of the original images, so they repeated all the relevant experiments and they state that the results are the same as the published data. The authors thus stand by the overall conclusion of the study.

New Journal of Chemistry

A rapid, sensitive and accessible HPLC-UV method was developed and validated for simultaneous det... more A rapid, sensitive and accessible HPLC-UV method was developed and validated for simultaneous detection of CNAD and CA up to 1.0 ng ml−1 in plasma samples.