Rui Pedrosa - Profile on Academia.edu (original) (raw)
Papers by Rui Pedrosa
Mechanisms associated to cytotoxicity and antimicrobial activities of VLC fractions isolated from brown seaweed Fucus spiralis
Frontiers in Marine Science, 2016
Lipids in Meat and Seafood
Antioxidant Small-spotted Catshark Burgers
Frontiers in Marine Science, 2014
The effects of food and photoperiod on strobilation of Aurelia aurita polyps
Frontiers in Marine Science, 2014
Angiotensin II and Cl‐/HCO3‐ Exchanger Activity in Immortalized SHR Proximal Tubular Epithelial Cells
The FASEB Journal, Mar 1, 2006
Mesoporous silica nanoparticles with manganese and lanthanide salts: synthesis, characterization and cytotoxicity studies
Dalton Transactions, 2021
Several organic salts based on the combination of two different choline derivative cations and Mn... more Several organic salts based on the combination of two different choline derivative cations and MnCl3−, GdCl4− and TbCl4− as anions were immobilized in mesoporous silica nanoparticles (MSNs) by a two-step synthetic method.
American Journal of Physiology-renal Physiology, Nov 1, 2004
This study evaluated the transduction pathway associated with type 3 Na ϩ /H ϩ exchanger (NHE3) a... more This study evaluated the transduction pathway associated with type 3 Na ϩ /H ϩ exchanger (NHE3) activity-induced inhibition during dopamine D3 receptor activation in immortalized renal proximal tubular epithelial cells from the spontaneously hypertensive rat. The dopamine D3 receptor agonist 7-OH-DPAT decreased NHE3 activity, which was prevented by the D2-like receptor antagonist S-sulpiride, pertussis toxin (PTX; overnight treatment), and the PKC inhibitor chelerythrine, but not by cholera toxin (overnight treatment), the MAPK inhibitor PD-098059, or the p38 inhibitor SB-203580. The PKA inhibitor H-89 abolished the inhibitory effects of forskolin on NHE3 activity, but not that of 7-OH-DPAT. The phospholipase C (PLC) inhibitor U-73122 prevented the inhibitory effects of 7-OH-DPAT, whereas PDBu and 7-OH-DPAT increased PLC activity and reduced NHE3 activity; downregulation of PKC abolished the inhibitory effects of both PDBu and 7-OH-DPAT on NHE activity. The inhibition of NHE3 activity by GTP␥S and the prevention of the effect of 7-OH-DPAT by PTX suggest an involvement of a G i/o protein coupled to the dopamine D3 receptor. Indeed, the 7-OH-DPAT-induced decrease in NHE3 activity was abolished in cells treated overnight with the anti-Gi␣3 antibody, but not in cells treated with antibodies against Gq/11, Gs␣, G, and Gi␣1,2 proteins. The calcium ionophore A-23187 and the Ca 2ϩ -ATPase inhibitor thapsigargin increased intracellular Ca 2ϩ but did not affect NHE3 activity. However, the inhibitory effects of PDBu and 7-OH-DPAT on NHE3 activity were completely abolished by A-23287 and thapsigargin. It is concluded that inhibition of NHE3 activity by dopamine D3 receptors coupled to Gi␣3 proteins is a PLC-PKC-mediated event, modulated by intracellular Ca 2ϩ . Na ϩ /H ϩ exchange; protein kinases; hypertension DOPAMINE PRODUCED BY RENAL proximal tubular cells exerts an autocrine/paracrine action via two classes of dopamine receptors, D 1 -like (D 1 and D 5 ) and D 2 -like (D 2 , D 3 , and D 4 ), which are differentially expressed along the nephron . The autocrine/paracrine function of dopamine, manifested by tubular rather than by hemodynamic mechanisms, becomes most evident during extracellular fluid volume expansion (25). This renal autocrine/paracrine function is lost in essential hypertension and in some animal models of genetic hypertension (4, 22-24, 28, 45, 56). Furthermore, disruption of the D 1 or D 3 receptor produces hypertension in mice (1, 5). In some humans
Effect of natural antioxidants on the quality of frozen sardine fillets (Sardina pilchardus)
Frontiers in Marine Science, 2014
Author response for "Valorisation of Sargassum muticum through the extraction of phenolic compounds using eutectic solvents and intensification techniques
Salted and dried Cod preserved by vacuum and modified atmosphere
Frontiers in Marine Science, 2014
Seaweed Extracts as Edible Coatings for Minimally Processed Products
Frontiers in Marine Science, 2014
LEDs light spectrum effect on the success of fragmentation and growth of the leather coral Sarcophyton spp
Frontiers in Marine Science, 2014
Fucus spiralis tissue culture for sustainable phlorotannins production
Aquatic Botany, 2022
Mitigating the negative impacts of marine invasive species – Sargassum muticum - a key seaweed for skincare products development
Algal Research, 2022
Cancer and infectious diseases continue to be a major public health problem, and new drugs are ne... more Cancer and infectious diseases continue to be a major public health problem, and new drugs are necessary. As marine organisms are well known to provide a wide range of original compounds, the aim of this study was to investigate the bioactivity of the main constituents of the cosmopolitan red alga, Sphaerococcus coronopifolius. The structure of several bromoditerpenes was determined by extensive spectroscopic analysis and comparison with literature data. Five molecules were isolated and characterized which include a new brominated diterpene belonging to the rare dactylomelane family and named sphaerodactylomelol (1), along with four already known sphaerane bromoditerpenes (2-5). Antitumor activity was assessed by cytotoxicity and anti-proliferative assays on an in vitro OPEN ACCESS Mar. Drugs 2015, 13 714 model of human hepatocellular carcinoma (HepG-2 cells). Antimicrobial activity was evaluated against four pathogenic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Compound 4 exhibited the highest antimicrobial activity against S. aureus (IC50 6.35 µM) and compound 5 the highest anti-proliferative activity on HepG-2 cells (IC50 42.9 µM). The new diterpene, sphaerodactylomelol (1), induced inhibition of cell proliferation (IC50 280 µM) and cytotoxicity (IC50 720 µM) on HepG-2 cells and showed antimicrobial activity against S. aureus (IC50 96.3 µM).
Preservation of fresh-cut Rocha Pear using Codium tomentosum extract
LWT, 2022
Brown Seaweeds
Nonvitamin and Nonmineral Nutritional Supplements, 2019
Bifurcaria bifurcata:生物活性化合物及び機能性成分の源としての重要な大型藻類【Powered by NICT】
International Journal of Food Science and Technology, 2016
Algal Research, 2020
Infectious diseases remain one of the leading global causes of death, mainly due to the increasin... more Infectious diseases remain one of the leading global causes of death, mainly due to the increasing number of multi-resistant microorganisms. Therefore, novel antimicrobials are continuously needed. Marine organisms have already proven to be a rich source of bioactive compounds which can be used for the development of novel pharmacological drugs. Within these, seaweeds are an important resource still underexplored. Asparagopsis armata is a marine invasive seaweed that has spread along several coastlines of the world, inducing negative pressures in marine ecosystems. Therefore, it is crucial to develop strategies to counteract their impacts. In this work, A. armata was extracted with methanol and dichloromethane (1:1) and fractionated by column chromatography with different polarity solvents, providing 8 fractions (F1-F8). All the fractions were evaluated for their antimicrobial potential against important human pathogens, namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, and Candida albicans. Additionally, their capacity to inhibit biofilms formation and the effects on cytoplasmatic membrane and DNA integrity were also assessed. The most active fractions were further purified and submitted to GC-MS analysis. The results showed that A. armata produces compounds with a high inhibitory activity against S. aureus, P. aeruginosa, and C. albicans growth, possibly mediated by cytoplasmatic membrane disruption and DNA damage. GC-MS analysis suggested that the most active fractions were mainly composed of bromoditerpenes and fatty acids. The attained results point to the relevance of the invasive A. armata as a source of antimicrobial substances with broad-spectrum activity. The use of invasive species to obtain natural bioactive compounds presents a two-folded opportunity -high availability of the biological material for the extraction of bioactive compounds and, through specimen collection, the mitigation of the negative effects caused by invasive species, contributing to ecosystem integrity and sustainability.
Science of The Total Environment, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Mechanisms associated to cytotoxicity and antimicrobial activities of VLC fractions isolated from brown seaweed Fucus spiralis
Frontiers in Marine Science, 2016
Lipids in Meat and Seafood
Antioxidant Small-spotted Catshark Burgers
Frontiers in Marine Science, 2014
The effects of food and photoperiod on strobilation of Aurelia aurita polyps
Frontiers in Marine Science, 2014
Angiotensin II and Cl‐/HCO3‐ Exchanger Activity in Immortalized SHR Proximal Tubular Epithelial Cells
The FASEB Journal, Mar 1, 2006
Mesoporous silica nanoparticles with manganese and lanthanide salts: synthesis, characterization and cytotoxicity studies
Dalton Transactions, 2021
Several organic salts based on the combination of two different choline derivative cations and Mn... more Several organic salts based on the combination of two different choline derivative cations and MnCl3−, GdCl4− and TbCl4− as anions were immobilized in mesoporous silica nanoparticles (MSNs) by a two-step synthetic method.
American Journal of Physiology-renal Physiology, Nov 1, 2004
This study evaluated the transduction pathway associated with type 3 Na ϩ /H ϩ exchanger (NHE3) a... more This study evaluated the transduction pathway associated with type 3 Na ϩ /H ϩ exchanger (NHE3) activity-induced inhibition during dopamine D3 receptor activation in immortalized renal proximal tubular epithelial cells from the spontaneously hypertensive rat. The dopamine D3 receptor agonist 7-OH-DPAT decreased NHE3 activity, which was prevented by the D2-like receptor antagonist S-sulpiride, pertussis toxin (PTX; overnight treatment), and the PKC inhibitor chelerythrine, but not by cholera toxin (overnight treatment), the MAPK inhibitor PD-098059, or the p38 inhibitor SB-203580. The PKA inhibitor H-89 abolished the inhibitory effects of forskolin on NHE3 activity, but not that of 7-OH-DPAT. The phospholipase C (PLC) inhibitor U-73122 prevented the inhibitory effects of 7-OH-DPAT, whereas PDBu and 7-OH-DPAT increased PLC activity and reduced NHE3 activity; downregulation of PKC abolished the inhibitory effects of both PDBu and 7-OH-DPAT on NHE activity. The inhibition of NHE3 activity by GTP␥S and the prevention of the effect of 7-OH-DPAT by PTX suggest an involvement of a G i/o protein coupled to the dopamine D3 receptor. Indeed, the 7-OH-DPAT-induced decrease in NHE3 activity was abolished in cells treated overnight with the anti-Gi␣3 antibody, but not in cells treated with antibodies against Gq/11, Gs␣, G, and Gi␣1,2 proteins. The calcium ionophore A-23187 and the Ca 2ϩ -ATPase inhibitor thapsigargin increased intracellular Ca 2ϩ but did not affect NHE3 activity. However, the inhibitory effects of PDBu and 7-OH-DPAT on NHE3 activity were completely abolished by A-23287 and thapsigargin. It is concluded that inhibition of NHE3 activity by dopamine D3 receptors coupled to Gi␣3 proteins is a PLC-PKC-mediated event, modulated by intracellular Ca 2ϩ . Na ϩ /H ϩ exchange; protein kinases; hypertension DOPAMINE PRODUCED BY RENAL proximal tubular cells exerts an autocrine/paracrine action via two classes of dopamine receptors, D 1 -like (D 1 and D 5 ) and D 2 -like (D 2 , D 3 , and D 4 ), which are differentially expressed along the nephron . The autocrine/paracrine function of dopamine, manifested by tubular rather than by hemodynamic mechanisms, becomes most evident during extracellular fluid volume expansion (25). This renal autocrine/paracrine function is lost in essential hypertension and in some animal models of genetic hypertension (4, 22-24, 28, 45, 56). Furthermore, disruption of the D 1 or D 3 receptor produces hypertension in mice (1, 5). In some humans
Effect of natural antioxidants on the quality of frozen sardine fillets (Sardina pilchardus)
Frontiers in Marine Science, 2014
Author response for "Valorisation of Sargassum muticum through the extraction of phenolic compounds using eutectic solvents and intensification techniques
Salted and dried Cod preserved by vacuum and modified atmosphere
Frontiers in Marine Science, 2014
Seaweed Extracts as Edible Coatings for Minimally Processed Products
Frontiers in Marine Science, 2014
LEDs light spectrum effect on the success of fragmentation and growth of the leather coral Sarcophyton spp
Frontiers in Marine Science, 2014
Fucus spiralis tissue culture for sustainable phlorotannins production
Aquatic Botany, 2022
Mitigating the negative impacts of marine invasive species – Sargassum muticum - a key seaweed for skincare products development
Algal Research, 2022
Cancer and infectious diseases continue to be a major public health problem, and new drugs are ne... more Cancer and infectious diseases continue to be a major public health problem, and new drugs are necessary. As marine organisms are well known to provide a wide range of original compounds, the aim of this study was to investigate the bioactivity of the main constituents of the cosmopolitan red alga, Sphaerococcus coronopifolius. The structure of several bromoditerpenes was determined by extensive spectroscopic analysis and comparison with literature data. Five molecules were isolated and characterized which include a new brominated diterpene belonging to the rare dactylomelane family and named sphaerodactylomelol (1), along with four already known sphaerane bromoditerpenes (2-5). Antitumor activity was assessed by cytotoxicity and anti-proliferative assays on an in vitro OPEN ACCESS Mar. Drugs 2015, 13 714 model of human hepatocellular carcinoma (HepG-2 cells). Antimicrobial activity was evaluated against four pathogenic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Compound 4 exhibited the highest antimicrobial activity against S. aureus (IC50 6.35 µM) and compound 5 the highest anti-proliferative activity on HepG-2 cells (IC50 42.9 µM). The new diterpene, sphaerodactylomelol (1), induced inhibition of cell proliferation (IC50 280 µM) and cytotoxicity (IC50 720 µM) on HepG-2 cells and showed antimicrobial activity against S. aureus (IC50 96.3 µM).
Preservation of fresh-cut Rocha Pear using Codium tomentosum extract
LWT, 2022
Brown Seaweeds
Nonvitamin and Nonmineral Nutritional Supplements, 2019
Bifurcaria bifurcata:生物活性化合物及び機能性成分の源としての重要な大型藻類【Powered by NICT】
International Journal of Food Science and Technology, 2016
Algal Research, 2020
Infectious diseases remain one of the leading global causes of death, mainly due to the increasin... more Infectious diseases remain one of the leading global causes of death, mainly due to the increasing number of multi-resistant microorganisms. Therefore, novel antimicrobials are continuously needed. Marine organisms have already proven to be a rich source of bioactive compounds which can be used for the development of novel pharmacological drugs. Within these, seaweeds are an important resource still underexplored. Asparagopsis armata is a marine invasive seaweed that has spread along several coastlines of the world, inducing negative pressures in marine ecosystems. Therefore, it is crucial to develop strategies to counteract their impacts. In this work, A. armata was extracted with methanol and dichloromethane (1:1) and fractionated by column chromatography with different polarity solvents, providing 8 fractions (F1-F8). All the fractions were evaluated for their antimicrobial potential against important human pathogens, namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, and Candida albicans. Additionally, their capacity to inhibit biofilms formation and the effects on cytoplasmatic membrane and DNA integrity were also assessed. The most active fractions were further purified and submitted to GC-MS analysis. The results showed that A. armata produces compounds with a high inhibitory activity against S. aureus, P. aeruginosa, and C. albicans growth, possibly mediated by cytoplasmatic membrane disruption and DNA damage. GC-MS analysis suggested that the most active fractions were mainly composed of bromoditerpenes and fatty acids. The attained results point to the relevance of the invasive A. armata as a source of antimicrobial substances with broad-spectrum activity. The use of invasive species to obtain natural bioactive compounds presents a two-folded opportunity -high availability of the biological material for the extraction of bioactive compounds and, through specimen collection, the mitigation of the negative effects caused by invasive species, contributing to ecosystem integrity and sustainability.
Science of The Total Environment, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.