SADHAN BAG - Academia.edu (original) (raw)

Papers by SADHAN BAG

Research paper thumbnail of Expression profile of H19 and Peg1 among diploid parthenogenetic, female sexed IVF and in vivo derived embryos during pre-implantation development in goat

Research paper thumbnail of Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultu...

Theriogenology, 2015

This study investigated the expression and localization of insulin-like growth factor (IGF) syste... more This study investigated the expression and localization of insulin-like growth factor (IGF) system at different stages of buffalo CL and the role of IGF-I in stimulating vascular endothelial growth factor (VEGF) and progesterone (P4) production in cultured luteal cells. The mRNA expression of IGF system, VEGF, steroidogenic acute regulatory protein, P450scc, and hydroxysteroid dehydrogenase (HSD) was investigated by quantitative real-time polymerase chain reaction (PCR). Protein expression of IGF was demonstrated by Western blot and localization by immunohistochemistry. Progesterone and VEGF production was assayed using RIA and ELISA. A relatively high mRNA expression of IGF-I and IGF-II in early, mid-and late luteal phases with immunoreactivity mostly restricted to cytoplasm of large luteal cells indicates their autocrine role, whereas very weak immunoreactivity in endothelial cells during the midluteal phase indicates their paracrine role. Insulin-like growth factor receptors, IGF-IR and IGF-IIR, were restricted to large luteal cells with high mRNA and protein expressions in the mid-luteal phase. The significantly higher expression of insulin-like growth factor binding protein (IGFBP)-1,-3,-5, and-6 in the early or mid-luteal phase suggested their stimulatory role, whereas that of IGFBP-2 and-4 in mid-, late, and regressive luteal stages implied their inhibitory role. The mRNA expressions of key steroidogenic factors and VEGF were significantly higher (P < 0.05) when the culture medium was supplemented with 100 ng/mL of IGF-I for 72 hours. Moreover, IGF-I at a dose of 100 ng/mL increased P4 and VEGF production (P < 0.05). It can be concluded that IGF family members via their autocrine and paracrine effect play significant roles in promoting angiogenesis through the production of VEGF in luteal cells and steroid synthesis through the production of key steroidogenic factors.

Research paper thumbnail of Fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor A (VEGFA) synergistically promote steroidogenesis and survival of cultured buffalo granulosa cells

Animal Reproduction Science, Apr 1, 2017

The present study investigated the combined effect of fibroblast growth factor 2 (FGF2) and vascu... more The present study investigated the combined effect of fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor A (VEGF-A) on estradiol (E 2) secretion and relative abundance of mRNA for aromatase enzyme (CYP19A1), proliferating cell nuclear antigen (PCNA) and BCL-2 associated X protein (BAX) in cultured buffalo granulosa cells (GCs). Follicles were isolated and classified into four groups based on size and E 2 concentration in follicular fluid (FF): Small, 4-6 mm diameter, E 2 < 0.5 ng/ml; Medium, 7-9 mm, E 2 = 0.5-5 ng/ml; Large, 10-13 mm, E 2 = 5-40 ng/ml; Preovulatory (PFs), >14 mm, E 2 > 180 ng/ml. The GCs of PF were cultured in 24 well cell culture plates and allowed to become 75% to 80% confluent. Then cultured GCs were treated with FGF2 (200 ng/ml) and VEGF-A (100 ng/ml) separately and in combination for three incubation periods (24, 48 and 72 h). Estradiol secretion was greater in GCs treated with FGF2+VEGF-A compared to FGF2 or VEGF-A at all incubation periods and was greatest (P<0.05) at 72 h of incubation. The relative abundance of CYP19A1 and PCNA mRNA were relatively consistent with the amount E 2 secretion. In contrast, the relative abundance of Bax mRNA was less in GCs treated with the combination of FGF2 and VEGF-A as compared to either FGF2 or VEGF-A alone and the least concentration (P<0.05) was at 72 h of incubation. Findings with use of immunocytochemistry of cells treated with these factors were consistent to the relative abundance of mRNA transcript for the factor. The present findings indicate that FGF2 and VEGF-A may function in a synergistic manner to promote steroidogenesis and survival of cultured buffalo GCs.

Research paper thumbnail of Sperm Function Assays

Springer eBooks, 2017

Evaluation of sperm function provides finality to the battery of in vitro assays while discrimina... more Evaluation of sperm function provides finality to the battery of in vitro assays while discriminating between a good from that of bad quality semen sample. In this important chapter, we have described zona binding assay with four types of variations, namely, hemizona, zona free, heterologous and homologous zona binding assays. Merits and demerits of these assays have been discussed here.

Research paper thumbnail of Role of embryonic stem cell-hydroxyapatite construct with growth proteins for osteogenesis in the repair of bone defects in rabbit model

Journal of Stem Cell Research & Therapeutics, Aug 16, 2018

Embryonic stem cells (ESC) represent an attracting cell population for tissue engineering purpose... more Embryonic stem cells (ESC) represent an attracting cell population for tissue engineering purpose. The objective of this study was to determine whether the addition of bone morphogenetic protein (BMP-2), transforming growth factor-β and insulin-like growth factor (IGF-1) to a calcium hydroxyapatite (HA-TCP)-rabbit embryonic stem cell (rESC) construct promoted bone healing in a segmental bone defect in rabbits. A 5mm long radial ostectomy was performed unilaterally in thirty-six rabbits divided equally into six groups. Defects were filled with a cell-free HA-TCP bioceramic scaffold only (group B); HA-TCP scaffold seeded with rESC (group C); HA-TCP scaffold seeded with rESC along with BMP-2, TGF-β and IGF-1 in groups D, E, and F respectively. An empty defect served as the control group (group A). Radiographically, bone healing was evaluated at 10, 20, 30 and 40days post implantation. Histological qualitative analysis by haematoxylin and eosin (H&E) staining and scanning electron microscopic (SEM) examination was performed 40 days after implantation. Radiographic and histological observation depicted incipient bone formation and filling the defect was more expeditious and better in ESC group than control and bioceramic groups. However, overall faster and better rate of bone healing was observed in the growth proteins added constructs groups (D, E and F) at day 40 after surgery as compared to other groups could be attributed to the application of growth factors in these groups along with the additive effect of the transplanted embryonic stem cells.

Research paper thumbnail of Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions

Research paper thumbnail of Isolation and characterization of neural stem cells from caprine

Indian Journal of Animal Sciences, 2013

Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transpl... more Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transplantation is impractical because it is hard to find a brain donor, and because of the low survival rate of grafted neural cells. Therefore, the need for constructing a functional brain tissue is very critical. In this study neural stem cells were isolated and expanded from caprine fetal brain in adherent culture. After 4–5 days of culture there were formation of small colonies and after about 10 days cells become confluent. Cells from passage 1 to 4 were used in study. These neural stem cells were characterized with neural stem cell specific markers nestin (NES), PAX6 and SOX2 by PCR and immunocytochemistry. The cells were expressed neural stem cell markers nestin, pax 6 and Sox 2.

Research paper thumbnail of Studies on blood acid-base status and muscle metabolic changes in workig buffaloes

Haryana and crossbred (Holstein x local Haryana) bullocks were subjected to work under heavy load... more Haryana and crossbred (Holstein x local Haryana) bullocks were subjected to work under heavy loads in summer. During the work, bullocks exhibited distress symptoms. After work, rectal temperature, respiration rate, heart rate and minute volume increased significantly, the average pO 2 content increased, muscle lactic acid increased and creatine phosphate level declined. From the results it was evident that oxygen availability in blood improved during work. Despite the enhanced oxygenation of blood, there was accumulation of lactic acid in muscle. This indicated a certain degree of tissue hypoxia, which probably brought about fatigue earlier.

Research paper thumbnail of Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides

Veterinary Research Communications, Apr 15, 2023

Research paper thumbnail of Effect of different serum concentrations on growth and storage of caprine bone marrow mesenchymal stem cells

Indian journal of animal health, 2016

Research paper thumbnail of Derivation Of ES Cells From Early Stage Preimplantation Embryos And Characterisation Of Their Cardiac Differentiation Potential In Mice

Journal of Stem Cells & Regenerative Medicine, Apr 11, 2012

Research paper thumbnail of Comparative expression profile of hexokinase gene in pre-implantation in vitro embryos in buffalo

The Indian Journal of Animal Sciences

Research paper thumbnail of Canine umbilical cord tissue derived mesenchymal stem cells naturally express some of the antimicrobial peptides

Research Square (Research Square), Nov 30, 2022

Antimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive ra... more Antimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive rate. They represent the rst line of active defence systems against invading microorganisms, helping in innate immunity. Besides their therapeutic applications, great attention has also been given to the mesenchymal stem cells (MSCs) due to their antimicrobial activities. The study aimed to observe the expression pro le of few antimicrobial peptides (AMPs) in canine MSCs during standard in vitro culture. MSCs were isolated from canine umbilical cord tissue, propagated and characterized by morphology, surface markers and tri-lineage differentiation capability. The mRNA expression of eleven commonly known antimicrobial peptides was checked by Reverse Transcriptase PCR. It has been found for the rst time that canine MSCs naturally express the AMPs like CXCL8, Ela n, Hepcidin, Lipocalin 2 and SLPI. However, their relation with antimicrobial effect of canine MSCs needs to be explored.

Research paper thumbnail of Nano-depletion of morbid spermatozoa up-regulate Ca2+ channel, depolarization of membrane potential and fertility in buffalo

Research paper thumbnail of Evaluation of cryopreservation efficiency on embryo growth after exposing them in intracellular cryoprotectant ethylene glycol or glycerol and replacing them by trehalose

The Indian Journal of Animal Sciences

The present study was conducted to see whether toxicity could be reduced by replacing some of the... more The present study was conducted to see whether toxicity could be reduced by replacing some of the penetrating cryoprotectant (intracellular cryoprotectant) with a disaccharide as well as finding out the optimum equilibration time in a vitrification solution before rapid cooling. Goat ovaries (1,685) were aspirated and 1,761 culturable oocytes (COC) were recovered. The recovered COCs were matured in maturation medium and evaluated on the basis of cumulus expansion. The matured oocytes were coincubated with fresh semen capacitated in TALP media, for 18 h. The inseminated oocytes were further cultured in mSOF for in vitro embryo development. The cleavage rate was 11.76% and the development rate of embryos to 4–8 and 8–16 cells morulae was 55.6% and 42.4%, respectively. In vitro produced goat embryos (132), 4–16 cell stages, were used for cryopreservation using different protocols. In protocol- 1, 2, 3 and 4, there was no survivability of embryos. In protocol- 5, 6, 7 and 8, the surviva...

Research paper thumbnail of Expression and functional role of IGFs during early pregnancy in placenta of water buffalo

Theriogenology, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Exogenous cholesterol prevents cryocapacitation‐like changes, membrane fluidity, and enhances in vitro fertility in bubaline spermatozoa

Reproduction in Domestic Animals, 2020

Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-v... more Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-vitro fertility in bubaline spermatozoa

Research paper thumbnail of Expression and functional role of fibroblast growth factors (FGF) in placenta during different stages of pregnancy in water buffalo (Bubalus bubalis)

Theriogenology, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Flaxseed oil modulates semen production and its quality profiles, freezability, testicular biometrics and endocrinological profiles in mithun

Research paper thumbnail of Effects of low-density lipoproteins as additive on quality parameters and oxidative stress following cryopreservation of mithun (Bos frontalis) spermatozoa

Reproduction in domestic animals = Zuchthygiene, Jan 14, 2016

Artificial breeding of mithun poses several challenges including lack of standard protocol for cr... more Artificial breeding of mithun poses several challenges including lack of standard protocol for cryopreservation of spermatozoa. This is further complicated by harmful effects of hen's egg yolk (EY) as additive in extender. Purified low-density lipoproteins (LDL) extracted from EY have been shown as beneficial over EY extender for long-term semen storage in several species. This investigation explored use of LDL versus EY on semen quality and oxidative stress following freezing-thawing of spermatozoa. A total of 25 of 50 ejaculates based on biophysical parameters were selected for the experiment. After diluting with the Tris-citrate-glycerol (TCG) extender, each sample was split into three equal aliquots: Group I, control, EY; Group II and Group III contained 8% and 10% purified LDL, respectively. Frozen-thawed samples were evaluated for motility parameters (progressive, and in the bovine cervical mucus penetration test [BCMPT]), viability, sperm and nuclear abnormality, acrosome...

Research paper thumbnail of Expression profile of H19 and Peg1 among diploid parthenogenetic, female sexed IVF and in vivo derived embryos during pre-implantation development in goat

Research paper thumbnail of Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultu...

Theriogenology, 2015

This study investigated the expression and localization of insulin-like growth factor (IGF) syste... more This study investigated the expression and localization of insulin-like growth factor (IGF) system at different stages of buffalo CL and the role of IGF-I in stimulating vascular endothelial growth factor (VEGF) and progesterone (P4) production in cultured luteal cells. The mRNA expression of IGF system, VEGF, steroidogenic acute regulatory protein, P450scc, and hydroxysteroid dehydrogenase (HSD) was investigated by quantitative real-time polymerase chain reaction (PCR). Protein expression of IGF was demonstrated by Western blot and localization by immunohistochemistry. Progesterone and VEGF production was assayed using RIA and ELISA. A relatively high mRNA expression of IGF-I and IGF-II in early, mid-and late luteal phases with immunoreactivity mostly restricted to cytoplasm of large luteal cells indicates their autocrine role, whereas very weak immunoreactivity in endothelial cells during the midluteal phase indicates their paracrine role. Insulin-like growth factor receptors, IGF-IR and IGF-IIR, were restricted to large luteal cells with high mRNA and protein expressions in the mid-luteal phase. The significantly higher expression of insulin-like growth factor binding protein (IGFBP)-1,-3,-5, and-6 in the early or mid-luteal phase suggested their stimulatory role, whereas that of IGFBP-2 and-4 in mid-, late, and regressive luteal stages implied their inhibitory role. The mRNA expressions of key steroidogenic factors and VEGF were significantly higher (P < 0.05) when the culture medium was supplemented with 100 ng/mL of IGF-I for 72 hours. Moreover, IGF-I at a dose of 100 ng/mL increased P4 and VEGF production (P < 0.05). It can be concluded that IGF family members via their autocrine and paracrine effect play significant roles in promoting angiogenesis through the production of VEGF in luteal cells and steroid synthesis through the production of key steroidogenic factors.

Research paper thumbnail of Fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor A (VEGFA) synergistically promote steroidogenesis and survival of cultured buffalo granulosa cells

Animal Reproduction Science, Apr 1, 2017

The present study investigated the combined effect of fibroblast growth factor 2 (FGF2) and vascu... more The present study investigated the combined effect of fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor A (VEGF-A) on estradiol (E 2) secretion and relative abundance of mRNA for aromatase enzyme (CYP19A1), proliferating cell nuclear antigen (PCNA) and BCL-2 associated X protein (BAX) in cultured buffalo granulosa cells (GCs). Follicles were isolated and classified into four groups based on size and E 2 concentration in follicular fluid (FF): Small, 4-6 mm diameter, E 2 < 0.5 ng/ml; Medium, 7-9 mm, E 2 = 0.5-5 ng/ml; Large, 10-13 mm, E 2 = 5-40 ng/ml; Preovulatory (PFs), >14 mm, E 2 > 180 ng/ml. The GCs of PF were cultured in 24 well cell culture plates and allowed to become 75% to 80% confluent. Then cultured GCs were treated with FGF2 (200 ng/ml) and VEGF-A (100 ng/ml) separately and in combination for three incubation periods (24, 48 and 72 h). Estradiol secretion was greater in GCs treated with FGF2+VEGF-A compared to FGF2 or VEGF-A at all incubation periods and was greatest (P<0.05) at 72 h of incubation. The relative abundance of CYP19A1 and PCNA mRNA were relatively consistent with the amount E 2 secretion. In contrast, the relative abundance of Bax mRNA was less in GCs treated with the combination of FGF2 and VEGF-A as compared to either FGF2 or VEGF-A alone and the least concentration (P<0.05) was at 72 h of incubation. Findings with use of immunocytochemistry of cells treated with these factors were consistent to the relative abundance of mRNA transcript for the factor. The present findings indicate that FGF2 and VEGF-A may function in a synergistic manner to promote steroidogenesis and survival of cultured buffalo GCs.

Research paper thumbnail of Sperm Function Assays

Springer eBooks, 2017

Evaluation of sperm function provides finality to the battery of in vitro assays while discrimina... more Evaluation of sperm function provides finality to the battery of in vitro assays while discriminating between a good from that of bad quality semen sample. In this important chapter, we have described zona binding assay with four types of variations, namely, hemizona, zona free, heterologous and homologous zona binding assays. Merits and demerits of these assays have been discussed here.

Research paper thumbnail of Role of embryonic stem cell-hydroxyapatite construct with growth proteins for osteogenesis in the repair of bone defects in rabbit model

Journal of Stem Cell Research & Therapeutics, Aug 16, 2018

Embryonic stem cells (ESC) represent an attracting cell population for tissue engineering purpose... more Embryonic stem cells (ESC) represent an attracting cell population for tissue engineering purpose. The objective of this study was to determine whether the addition of bone morphogenetic protein (BMP-2), transforming growth factor-β and insulin-like growth factor (IGF-1) to a calcium hydroxyapatite (HA-TCP)-rabbit embryonic stem cell (rESC) construct promoted bone healing in a segmental bone defect in rabbits. A 5mm long radial ostectomy was performed unilaterally in thirty-six rabbits divided equally into six groups. Defects were filled with a cell-free HA-TCP bioceramic scaffold only (group B); HA-TCP scaffold seeded with rESC (group C); HA-TCP scaffold seeded with rESC along with BMP-2, TGF-β and IGF-1 in groups D, E, and F respectively. An empty defect served as the control group (group A). Radiographically, bone healing was evaluated at 10, 20, 30 and 40days post implantation. Histological qualitative analysis by haematoxylin and eosin (H&E) staining and scanning electron microscopic (SEM) examination was performed 40 days after implantation. Radiographic and histological observation depicted incipient bone formation and filling the defect was more expeditious and better in ESC group than control and bioceramic groups. However, overall faster and better rate of bone healing was observed in the growth proteins added constructs groups (D, E and F) at day 40 after surgery as compared to other groups could be attributed to the application of growth factors in these groups along with the additive effect of the transplanted embryonic stem cells.

Research paper thumbnail of Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions

Research paper thumbnail of Isolation and characterization of neural stem cells from caprine

Indian Journal of Animal Sciences, 2013

Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transpl... more Central nervous system (CNS) has no or very little capacity to self repair. Neural tissue transplantation is impractical because it is hard to find a brain donor, and because of the low survival rate of grafted neural cells. Therefore, the need for constructing a functional brain tissue is very critical. In this study neural stem cells were isolated and expanded from caprine fetal brain in adherent culture. After 4–5 days of culture there were formation of small colonies and after about 10 days cells become confluent. Cells from passage 1 to 4 were used in study. These neural stem cells were characterized with neural stem cell specific markers nestin (NES), PAX6 and SOX2 by PCR and immunocytochemistry. The cells were expressed neural stem cell markers nestin, pax 6 and Sox 2.

Research paper thumbnail of Studies on blood acid-base status and muscle metabolic changes in workig buffaloes

Haryana and crossbred (Holstein x local Haryana) bullocks were subjected to work under heavy load... more Haryana and crossbred (Holstein x local Haryana) bullocks were subjected to work under heavy loads in summer. During the work, bullocks exhibited distress symptoms. After work, rectal temperature, respiration rate, heart rate and minute volume increased significantly, the average pO 2 content increased, muscle lactic acid increased and creatine phosphate level declined. From the results it was evident that oxygen availability in blood improved during work. Despite the enhanced oxygenation of blood, there was accumulation of lactic acid in muscle. This indicated a certain degree of tissue hypoxia, which probably brought about fatigue earlier.

Research paper thumbnail of Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides

Veterinary Research Communications, Apr 15, 2023

Research paper thumbnail of Effect of different serum concentrations on growth and storage of caprine bone marrow mesenchymal stem cells

Indian journal of animal health, 2016

Research paper thumbnail of Derivation Of ES Cells From Early Stage Preimplantation Embryos And Characterisation Of Their Cardiac Differentiation Potential In Mice

Journal of Stem Cells & Regenerative Medicine, Apr 11, 2012

Research paper thumbnail of Comparative expression profile of hexokinase gene in pre-implantation in vitro embryos in buffalo

The Indian Journal of Animal Sciences

Research paper thumbnail of Canine umbilical cord tissue derived mesenchymal stem cells naturally express some of the antimicrobial peptides

Research Square (Research Square), Nov 30, 2022

Antimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive ra... more Antimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive rate. They represent the rst line of active defence systems against invading microorganisms, helping in innate immunity. Besides their therapeutic applications, great attention has also been given to the mesenchymal stem cells (MSCs) due to their antimicrobial activities. The study aimed to observe the expression pro le of few antimicrobial peptides (AMPs) in canine MSCs during standard in vitro culture. MSCs were isolated from canine umbilical cord tissue, propagated and characterized by morphology, surface markers and tri-lineage differentiation capability. The mRNA expression of eleven commonly known antimicrobial peptides was checked by Reverse Transcriptase PCR. It has been found for the rst time that canine MSCs naturally express the AMPs like CXCL8, Ela n, Hepcidin, Lipocalin 2 and SLPI. However, their relation with antimicrobial effect of canine MSCs needs to be explored.

Research paper thumbnail of Nano-depletion of morbid spermatozoa up-regulate Ca2+ channel, depolarization of membrane potential and fertility in buffalo

Research paper thumbnail of Evaluation of cryopreservation efficiency on embryo growth after exposing them in intracellular cryoprotectant ethylene glycol or glycerol and replacing them by trehalose

The Indian Journal of Animal Sciences

The present study was conducted to see whether toxicity could be reduced by replacing some of the... more The present study was conducted to see whether toxicity could be reduced by replacing some of the penetrating cryoprotectant (intracellular cryoprotectant) with a disaccharide as well as finding out the optimum equilibration time in a vitrification solution before rapid cooling. Goat ovaries (1,685) were aspirated and 1,761 culturable oocytes (COC) were recovered. The recovered COCs were matured in maturation medium and evaluated on the basis of cumulus expansion. The matured oocytes were coincubated with fresh semen capacitated in TALP media, for 18 h. The inseminated oocytes were further cultured in mSOF for in vitro embryo development. The cleavage rate was 11.76% and the development rate of embryos to 4–8 and 8–16 cells morulae was 55.6% and 42.4%, respectively. In vitro produced goat embryos (132), 4–16 cell stages, were used for cryopreservation using different protocols. In protocol- 1, 2, 3 and 4, there was no survivability of embryos. In protocol- 5, 6, 7 and 8, the surviva...

Research paper thumbnail of Expression and functional role of IGFs during early pregnancy in placenta of water buffalo

Theriogenology, 2021

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Exogenous cholesterol prevents cryocapacitation‐like changes, membrane fluidity, and enhances in vitro fertility in bubaline spermatozoa

Reproduction in Domestic Animals, 2020

Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-v... more Exogenous cholesterol prevents cryocapacitation like changes, membrane fluidity and enhances in-vitro fertility in bubaline spermatozoa

Research paper thumbnail of Expression and functional role of fibroblast growth factors (FGF) in placenta during different stages of pregnancy in water buffalo (Bubalus bubalis)

Theriogenology, 2019

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Research paper thumbnail of Flaxseed oil modulates semen production and its quality profiles, freezability, testicular biometrics and endocrinological profiles in mithun

Research paper thumbnail of Effects of low-density lipoproteins as additive on quality parameters and oxidative stress following cryopreservation of mithun (Bos frontalis) spermatozoa

Reproduction in domestic animals = Zuchthygiene, Jan 14, 2016

Artificial breeding of mithun poses several challenges including lack of standard protocol for cr... more Artificial breeding of mithun poses several challenges including lack of standard protocol for cryopreservation of spermatozoa. This is further complicated by harmful effects of hen's egg yolk (EY) as additive in extender. Purified low-density lipoproteins (LDL) extracted from EY have been shown as beneficial over EY extender for long-term semen storage in several species. This investigation explored use of LDL versus EY on semen quality and oxidative stress following freezing-thawing of spermatozoa. A total of 25 of 50 ejaculates based on biophysical parameters were selected for the experiment. After diluting with the Tris-citrate-glycerol (TCG) extender, each sample was split into three equal aliquots: Group I, control, EY; Group II and Group III contained 8% and 10% purified LDL, respectively. Frozen-thawed samples were evaluated for motility parameters (progressive, and in the bovine cervical mucus penetration test [BCMPT]), viability, sperm and nuclear abnormality, acrosome...