SAMIA REJIBA - Academia.edu (original) (raw)

Papers by SAMIA REJIBA

Journal of Chemotherapy, Aug 1, 2007

Current Microbiology, Mar 29, 2023

Research Square (Research Square), Mar 30, 2023

Lactic acid bacteria and bi dobacteria (LAB & Bi do), isolated from the gastrointestinal tract of... more Lactic acid bacteria and bi dobacteria (LAB & Bi do), isolated from the gastrointestinal tract of Apis mellifera intermissa (BGIT), honey (H), propolis (P) and bee bread (BB) of hives set in different vegetations (wild owers, caraway, orange blossom, Marrubium vulgare, Eucalyptus and Erica cinerea), were subjected to analysis of their antibacterial potential. Isolates able to inhibit Staphylococcus aureus were selected and identi ed with MALDI-TOF MS leading to 154 strains representing 12 LAB & Bi do species. Lactiplantibacillus plantarum, Pediococcus pentosaceus and Enterococcus faecalis were predominantly found in all matrices. BGIT showed the highest LAB & Bi do diversity with exclusive occurrences of ve species (including Bi dobacterium asteroides and Limosilactobacillus fermentum). Honey was the second origin harboring an important variety of LAB species of which Apilactobacillus kunkeei and Enterococcus mundtii were characteristic of both H and BGIT. Principal components analysis revealed associations between antibacterial activities of LAB & Bi do, matrices and honey bee forage plants. Inhibition trends of S. aureus and Citrobacter freundii were highlighted with: Lactiplantibacillus plantarum from BGIT, P, H of bees feeding on Erica cinerea; Pediococcus pentosaceus from BGIT, P, BB associated with Erica cinerea; and Bi dobacterium asteroidesfrom BGIT/orange blossom system. However, Enterococcus faeciumassociated with BGIT/Eucalyptus system antagonized Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii. Our ndings highlighted noteworthy effects of bee forage plants on the antibacterial activity of LAB & Bi do. Our approach could be useful to identify multiple conditions promoting antibacterial potency of LAB & Bi do under the combined effects of feeding plants and living matrices.

Microbial Drug Resistance, Mar 1, 2002

Journal of Medical Microbiology, Feb 1, 2014

Journal of Chemotherapy, Dec 1, 2008

... REJIBA S. (1 2) ; AUBRY A. (3) ; PETITFRERE S. (4) ; JARLIER V. (3) ; CAMBAU E. (4 5) ; ... T... more ... REJIBA S. (1 2) ; AUBRY A. (3) ; PETITFRERE S. (4) ; JARLIER V. (3) ; CAMBAU E. (4 5) ; ... TUNISIE (2) Hôpital d'Enfants, 1006 Bab Saadoun, Laboratoire de Microbiologie, Tunis, TUNISIE (3) Université Paris 6, EA1541, Service de Bactériologie-Hygiène, CHU Pierre et Marie ...

Microbial Drug Resistance, Jun 1, 2011

Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected fro... more Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children's Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus-polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of bla TEM-1B (26 isolates, 81%), bla TEM-34(IRT-6) (3 isolates, 9%), bla SHV-12 (2 isolates, 6%), and bla CTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the bla CTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. bla TEM-1B and bla CTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both bla TEM-34(IRT-6) and bla CTX-M-15. bla SHV-12 was identified either alone or with bla CTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.

Research in Microbiology, Jul 1, 1999

The role of chromosomal cephalosporinases and secondary-lactamases in resistance to extended spec... more The role of chromosomal cephalosporinases and secondary-lactamases in resistance to extended spectrum cephalosporins in clinical isolates of Pseudomonas aeruginosa was investigated. Strains 687, 59, and 58 expressed an inducible chromosomal cephalosporinase, efficiently enhanced with cefoxitin and imipenem. The inducible activity in strain 802 was produced at a moderately elevated basal level and may be involved in resistance to extended spectrum cephalosporins and aztreonam. All strains produced secondary-lactamases inhibited by clavulanate: strains 687, 59, and 58 had carbenicillinases with pIs of 5.7 and 5.3. Strain 802 expressed a secondary-lactamase of pI 7.6 which may be a novel extended spectrum-lactamase different from known enzymes of P. aeruginosa.

Probiotics and Antimicrobial Proteins

This study aimed to isolate lactic acid bacteria (LAB) from the digestive tract, meat and slime o... more This study aimed to isolate lactic acid bacteria (LAB) from the digestive tract, meat and slime of edible snails (Helix lucorum, Helix aspersa and Eobania vermiculata) and investigate their antagonistic activity against Penicillium expansum. They were then characterized for their probiotic potential. Among 900 bacterial isolates, 47 LAB exhibiting anti-P. expansum activity were identified through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as Levilactobacillus brevis (25), Lactococcus lactis (3), Enterococcus faecium (12), Enterococcus faecalis (4), Enterococcus casseliflavus (1), and Enterococcus mundtii (2). Sixty-two percent of the strains were tolerant to 100 mg/L of lysozyme. Seventy two percent of the isolates were able to survive at pH 3 and most of them tolerate 2.5% bile salt concentration. Moreover, 23% of the strains displayed bile salt hydrolase activity. Interestingly, all strains were biofilm strong producers. However, their auto- and co-aggregation properties were time and pH dependent with high aggregative potentiality at pH 4.5 after 24 h. Remarkably, 48.94% of the strains showed high affinity to chloroform. The safety assessment revealed that the 47 LAB had no hemolytic activity and 64% of them lacked mucin degradation activity. All isolated strains were susceptible to gentamycin, streptomycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole. Overall, 43 LAB strains showed inhibitory activity against a broad spectrum of pathogenic Gram-positive and gram-negative bacteria, fungi, and yeast. Our findings suggest that L. brevis (EVM12 and EVM14) and Ent. faecium HAS34 strains could be potential candidates for probiotics with interesting antibacterial and anti-P. expansum activities.

Research in Microbiology, 1999

Microbial Drug Resistance, 2011

Microbial Drug Resistance, 2002