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The catheter related blood stream infections (CRBSI) are identified as a threat of human kind wor... more The catheter related blood stream infections (CRBSI) are identified as a threat of human kind worldwide affecting ICU admitted patients in hospitals. The rate of chances of getting the CRBSIs vary depending on the hospital set ups and several other variables. The aim of this study is to evaluate the percentage of bacterial colonisation and catheter related blood stream infections (CRBSI) together with the antibiotic susceptibility patterns in a tertiary care hospital located in Chennai, Tamilnadu. Of the 68 catheters studied out of 55 patients, the incidence of colonisation was found 37.6% and CRBSI was 14%. The pathogens identified from the cultures include S.aureus, Pneumonia sp. Klebisiella etc. Among this Staphylococcus aureus was found to be the major contributor of CRBSI with 25% rate of. With respect to antibacterial resistance 100% of Enterobacteriacae were ESBL producer, 49.0% Pseudomonas sp. were imipenem resistant, 65% Staphylococcus aureus were resistant to methicillin r...
Archives of Insect Biochemistry and Physiology, 2018
Aquaporins (AQPs) are integral membrane channels that facilitate the bidirectional transport of w... more Aquaporins (AQPs) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. AQPs are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. Here, we expressed Aedes aegypti AQP6 ( AaAQP6) in human embryonic kidney (HEK) 293 cells and analyzed the water permeability by a conventional swelling assay, that is, a real-time change in cell size corresponding to the cell swelling induced by hyposmotic solution. The swelling assay revealed that AaAQP6 is a mercury-sensitive water channel. Gene expression studies showed that AaAQP6 is highly expressed in the pupae than other developmental stages. Heterologous expression of AaAQP6 in HEK cell was mainly observed intracellularly suggesting AaAQP6 possibly could be a subcellular water channel and may play an osmoregulatory function in the pupae of A. aegypti.
Journal of receptor and signal transduction research, Jan 24, 2016
There has been a fair bit of understanding on the structure-function relationship of Aquaporins (... more There has been a fair bit of understanding on the structure-function relationship of Aquaporins (AQPs) from plants and vertebrates obtained from available X-ray crystallography data. However, there is a lacuna in understanding the structure of AQPs from sanguinivorous insects like the mosquito where it plays a crucial role in survival. In this study, we have built homology models for the Aedes aegypti AQPs, identified key channel lining residues and compared the structure and sequence with orthodox AQPs. Although Ar/R filter residues of AaAQP1 were exactly similar to orthodox AQPs, AaAQP2 has a substitution at LE1position possibly making it less efficient in high capacity water transport. The huge difference in the selectivity filter region of AaAQP3 suggests a different transport property for this channel. The changes observed in the H5 position of the filter of AaAQP4 and AaAQP5 may explain the presence of a larger pore aperture to permit the passage of larger solute molecules. Aa...
The catheter related blood stream infections (CRBSI) are identified as a threat of human kind wor... more The catheter related blood stream infections (CRBSI) are identified as a threat of human kind worldwide affecting ICU admitted patients in hospitals. The rate of chances of getting the CRBSIs vary depending on the hospital set ups and several other variables. The aim of this study is to evaluate the percentage of bacterial colonisation and catheter related blood stream infections (CRBSI) together with the antibiotic susceptibility patterns in a tertiary care hospital located in Chennai, Tamilnadu. Of the 68 catheters studied out of 55 patients, the incidence of colonisation was found 37.6% and CRBSI was 14%. The pathogens identified from the cultures include S.aureus, Pneumonia sp. Klebisiella etc. Among this Staphylococcus aureus was found to be the major contributor of CRBSI with 25% rate of. With respect to antibacterial resistance 100% of Enterobacteriacae were ESBL producer, 49.0% Pseudomonas sp. were imipenem resistant, 65% Staphylococcus aureus were resistant to methicillin r...
Archives of Insect Biochemistry and Physiology, 2018
Aquaporins (AQPs) are integral membrane channels that facilitate the bidirectional transport of w... more Aquaporins (AQPs) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. AQPs are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. Here, we expressed Aedes aegypti AQP6 ( AaAQP6) in human embryonic kidney (HEK) 293 cells and analyzed the water permeability by a conventional swelling assay, that is, a real-time change in cell size corresponding to the cell swelling induced by hyposmotic solution. The swelling assay revealed that AaAQP6 is a mercury-sensitive water channel. Gene expression studies showed that AaAQP6 is highly expressed in the pupae than other developmental stages. Heterologous expression of AaAQP6 in HEK cell was mainly observed intracellularly suggesting AaAQP6 possibly could be a subcellular water channel and may play an osmoregulatory function in the pupae of A. aegypti.
Journal of receptor and signal transduction research, Jan 24, 2016
There has been a fair bit of understanding on the structure-function relationship of Aquaporins (... more There has been a fair bit of understanding on the structure-function relationship of Aquaporins (AQPs) from plants and vertebrates obtained from available X-ray crystallography data. However, there is a lacuna in understanding the structure of AQPs from sanguinivorous insects like the mosquito where it plays a crucial role in survival. In this study, we have built homology models for the Aedes aegypti AQPs, identified key channel lining residues and compared the structure and sequence with orthodox AQPs. Although Ar/R filter residues of AaAQP1 were exactly similar to orthodox AQPs, AaAQP2 has a substitution at LE1position possibly making it less efficient in high capacity water transport. The huge difference in the selectivity filter region of AaAQP3 suggests a different transport property for this channel. The changes observed in the H5 position of the filter of AaAQP4 and AaAQP5 may explain the presence of a larger pore aperture to permit the passage of larger solute molecules. Aa...