SAYANI ROY - Academia.edu (original) (raw)

Papers by SAYANI ROY

Research paper thumbnail of Occurrence of atypical enteropathogenic Escherichia coli with multidrug-resistant trait in street food of Northeast India

Indian Journal of Public Health, 2022

The aim of this study was to investigate the genetic diversity and class 2 integron content of ty... more The aim of this study was to investigate the genetic diversity and class 2 integron content of typical and atypical enteropathogenic Escherichia coli (EPEC) strains isolated from children less than 5 years of age. Biochemical tests and serogrouping were performed for identification of isolated strains, and each isolate was tested for susceptibility to 7 antimicrobial agents. The identity of EPEC and their class 2 integron content was confirmed by PCR analysis and sequencing. Subtyping of Escherichia coli spp. was performed through pulsed-field gel electrophoresis (PFGE) analysis. All EPEC strains were resistant to 6 antimicrobial agents except for gentamycin. The most prevalent serogroups among EPEC strains were found to be members of O86 and O127 serogroups (37.7 %) and O44, O125, and O128 (42.8 %). The majority of our EPEC isolates (60.7 %) were identified as atypical. Among the total 28 isolates, 4 (14.2 %) harbored a class 2 integron 1,500 or 2,300 bp in size, corresponding to dfrA1-sat1 and dfrA1-sat1-aadA1 resistance gene cassette arrays, respectively. PFGE analysis showed an extensive diversity among the isolates. No PFGE clustering was observed according to bundle-forming pilus (bfp) bacteria, suggesting that PFGE analysis could not discriminate between typical and atypical EPEC strains. The high ratio of antibiotic-resistant strains and the large heterogeneity among EPEC isolates with low prevalence of class 2 integrons signify the need to examine for other mechanism(s) involved in conferring resistance in typical and atypical populations of EPEC.

Research paper thumbnail of Occurrence of virulent Serratia marcescens with co-existing antibiotic resistance determinants in ready-to-eat food samples

Journal of Microbiology and Infectious Diseases

Background: Foodborne illness is a global concern and identification of pathogens that cause food... more Background: Foodborne illness is a global concern and identification of pathogens that cause foodborne disease is a public health priority. Serratia marcescens is an opportunistic pathogen responsible for food poisoning due to its ability to colonize the gastrointestinal surfaces. S. marcescens is also responsible for causing a wide range of extraintestinal infections like pneumonia, urinary tract infection, keratitis, etc. Aim: The current study investigated the presence of antibiotic resistant virulent S. marcescens from street food samples. Methods: Two pigmented S. marcescens were recovered from Panipuri samples which were then identified using VITEK® GN cards. The virulence genes and the antibiotic resistance genes harboured by the S. marcescens isolates were investigated and further transformation assay was conducted to check the horizontal transferability of the plasmid carrying the antimicrobial resistance genes. Incompatibility typing of the transformants were also assessed...

Research paper thumbnail of The occurrence of atypical enteropathogenic Escherichia coli strains among children with diarrhea in Iran

Journal of Infection and Chemotherapy, 2013

The aim of this study was to investigate the genetic diversity and class 2 integron content of ty... more The aim of this study was to investigate the genetic diversity and class 2 integron content of typical and atypical enteropathogenic Escherichia coli (EPEC) strains isolated from children less than 5 years of age. Biochemical tests and serogrouping were performed for identification of isolated strains, and each isolate was tested for susceptibility to 7 antimicrobial agents. The identity of EPEC and their class 2 integron content was confirmed by PCR analysis and sequencing. Subtyping of Escherichia coli spp. was performed through pulsed-field gel electrophoresis (PFGE) analysis. All EPEC strains were resistant to 6 antimicrobial agents except for gentamycin. The most prevalent serogroups among EPEC strains were found to be members of O86 and O127 serogroups (37.7 %) and O44, O125, and O128 (42.8 %). The majority of our EPEC isolates (60.7 %) were identified as atypical. Among the total 28 isolates, 4 (14.2 %) harbored a class 2 integron 1,500 or 2,300 bp in size, corresponding to dfrA1-sat1 and dfrA1-sat1-aadA1 resistance gene cassette arrays, respectively. PFGE analysis showed an extensive diversity among the isolates. No PFGE clustering was observed according to bundle-forming pilus (bfp) bacteria, suggesting that PFGE analysis could not discriminate between typical and atypical EPEC strains. The high ratio of antibiotic-resistant strains and the large heterogeneity among EPEC isolates with low prevalence of class 2 integrons signify the need to examine for other mechanism(s) involved in conferring resistance in typical and atypical populations of EPEC.

Research paper thumbnail of Antiviral Phytocompounds: A Methodical Review of Therapeutic Efficiency Against SARS-Like Human Coronaviruses

Journal of Plant Biotechnology Research, 2020

Health Organization (WHO) [5]. Recently the emergence of a highly infectious beta-coronavirus, th... more Health Organization (WHO) [5]. Recently the emergence of a highly infectious beta-coronavirus, the SARS coronavirus 2 or

Research paper thumbnail of Transcriptional expression of PmrB and arnA within polymyxin-resistant nosocomial isolates of Pseudomonas aeruginosa from India

Journal of Microbiology and Infectious Diseases

Objective: The polymyxin group of antibiotics is considered to be one of the most effective antim... more Objective: The polymyxin group of antibiotics is considered to be one of the most effective antimicrobial agents against many serious pathogenic bacteria, but the excessive use of these antibiotics has led to the development of drug resistance among bacteria. This study was designed to characterize polymyxin-resistant P. aeruginosa and to explore the role of PmrB and arnA in resistant phenotype. Methods: mRNA and cDNA of five selected polymyxin-resistant strains representing different MIC range; isolated under normal condition of strain growth, after treating sample/media with FeCl3 and MgCl2 alone, or after treating with FeCl3 and polymyxin antibiotic. The transcriptional expression was observed for PmrB and arnA by quantitative real time RT-PCR in reference to P. aeruginosa PAO1. The presence of plasmid mediated colistin resistance determinants mcr-1 was screened by PCR. Susceptibility of the strains was determined by disc-diffusion method and DNA fingerprinting was carried out by performing REP-PCR. Results: A down regulated expression of PmrB and arnA was observed even after the unique induction with FeCl3 and MgCl2. All the isolates were found to be resistant against cefepime and different clonal patterns of resistance were found among the isolates. Conclusion: This study has drawn a new insight into polymyxin resistance which will help in the detection and control of infections caused by multidrug resistant P. aeruginosa. The low susceptibility rate to aminoglycoside, piperacllin-tazobactam and ciprofloxacin was found and in addition, detection of PmrB and arnA as molecular markers in the follow up of infections caused by multidrug resistant P. aeruginosa.

Research paper thumbnail of Occurrence of atypical enteropathogenic Escherichia coli with multidrug-resistant trait in street food of Northeast India

Indian Journal of Public Health, 2022

The aim of this study was to investigate the genetic diversity and class 2 integron content of ty... more The aim of this study was to investigate the genetic diversity and class 2 integron content of typical and atypical enteropathogenic Escherichia coli (EPEC) strains isolated from children less than 5 years of age. Biochemical tests and serogrouping were performed for identification of isolated strains, and each isolate was tested for susceptibility to 7 antimicrobial agents. The identity of EPEC and their class 2 integron content was confirmed by PCR analysis and sequencing. Subtyping of Escherichia coli spp. was performed through pulsed-field gel electrophoresis (PFGE) analysis. All EPEC strains were resistant to 6 antimicrobial agents except for gentamycin. The most prevalent serogroups among EPEC strains were found to be members of O86 and O127 serogroups (37.7 %) and O44, O125, and O128 (42.8 %). The majority of our EPEC isolates (60.7 %) were identified as atypical. Among the total 28 isolates, 4 (14.2 %) harbored a class 2 integron 1,500 or 2,300 bp in size, corresponding to dfrA1-sat1 and dfrA1-sat1-aadA1 resistance gene cassette arrays, respectively. PFGE analysis showed an extensive diversity among the isolates. No PFGE clustering was observed according to bundle-forming pilus (bfp) bacteria, suggesting that PFGE analysis could not discriminate between typical and atypical EPEC strains. The high ratio of antibiotic-resistant strains and the large heterogeneity among EPEC isolates with low prevalence of class 2 integrons signify the need to examine for other mechanism(s) involved in conferring resistance in typical and atypical populations of EPEC.

Research paper thumbnail of Occurrence of virulent Serratia marcescens with co-existing antibiotic resistance determinants in ready-to-eat food samples

Journal of Microbiology and Infectious Diseases

Background: Foodborne illness is a global concern and identification of pathogens that cause food... more Background: Foodborne illness is a global concern and identification of pathogens that cause foodborne disease is a public health priority. Serratia marcescens is an opportunistic pathogen responsible for food poisoning due to its ability to colonize the gastrointestinal surfaces. S. marcescens is also responsible for causing a wide range of extraintestinal infections like pneumonia, urinary tract infection, keratitis, etc. Aim: The current study investigated the presence of antibiotic resistant virulent S. marcescens from street food samples. Methods: Two pigmented S. marcescens were recovered from Panipuri samples which were then identified using VITEK® GN cards. The virulence genes and the antibiotic resistance genes harboured by the S. marcescens isolates were investigated and further transformation assay was conducted to check the horizontal transferability of the plasmid carrying the antimicrobial resistance genes. Incompatibility typing of the transformants were also assessed...

Research paper thumbnail of The occurrence of atypical enteropathogenic Escherichia coli strains among children with diarrhea in Iran

Journal of Infection and Chemotherapy, 2013

The aim of this study was to investigate the genetic diversity and class 2 integron content of ty... more The aim of this study was to investigate the genetic diversity and class 2 integron content of typical and atypical enteropathogenic Escherichia coli (EPEC) strains isolated from children less than 5 years of age. Biochemical tests and serogrouping were performed for identification of isolated strains, and each isolate was tested for susceptibility to 7 antimicrobial agents. The identity of EPEC and their class 2 integron content was confirmed by PCR analysis and sequencing. Subtyping of Escherichia coli spp. was performed through pulsed-field gel electrophoresis (PFGE) analysis. All EPEC strains were resistant to 6 antimicrobial agents except for gentamycin. The most prevalent serogroups among EPEC strains were found to be members of O86 and O127 serogroups (37.7 %) and O44, O125, and O128 (42.8 %). The majority of our EPEC isolates (60.7 %) were identified as atypical. Among the total 28 isolates, 4 (14.2 %) harbored a class 2 integron 1,500 or 2,300 bp in size, corresponding to dfrA1-sat1 and dfrA1-sat1-aadA1 resistance gene cassette arrays, respectively. PFGE analysis showed an extensive diversity among the isolates. No PFGE clustering was observed according to bundle-forming pilus (bfp) bacteria, suggesting that PFGE analysis could not discriminate between typical and atypical EPEC strains. The high ratio of antibiotic-resistant strains and the large heterogeneity among EPEC isolates with low prevalence of class 2 integrons signify the need to examine for other mechanism(s) involved in conferring resistance in typical and atypical populations of EPEC.

Research paper thumbnail of Antiviral Phytocompounds: A Methodical Review of Therapeutic Efficiency Against SARS-Like Human Coronaviruses

Journal of Plant Biotechnology Research, 2020

Health Organization (WHO) [5]. Recently the emergence of a highly infectious beta-coronavirus, th... more Health Organization (WHO) [5]. Recently the emergence of a highly infectious beta-coronavirus, the SARS coronavirus 2 or

Research paper thumbnail of Transcriptional expression of PmrB and arnA within polymyxin-resistant nosocomial isolates of Pseudomonas aeruginosa from India

Journal of Microbiology and Infectious Diseases

Objective: The polymyxin group of antibiotics is considered to be one of the most effective antim... more Objective: The polymyxin group of antibiotics is considered to be one of the most effective antimicrobial agents against many serious pathogenic bacteria, but the excessive use of these antibiotics has led to the development of drug resistance among bacteria. This study was designed to characterize polymyxin-resistant P. aeruginosa and to explore the role of PmrB and arnA in resistant phenotype. Methods: mRNA and cDNA of five selected polymyxin-resistant strains representing different MIC range; isolated under normal condition of strain growth, after treating sample/media with FeCl3 and MgCl2 alone, or after treating with FeCl3 and polymyxin antibiotic. The transcriptional expression was observed for PmrB and arnA by quantitative real time RT-PCR in reference to P. aeruginosa PAO1. The presence of plasmid mediated colistin resistance determinants mcr-1 was screened by PCR. Susceptibility of the strains was determined by disc-diffusion method and DNA fingerprinting was carried out by performing REP-PCR. Results: A down regulated expression of PmrB and arnA was observed even after the unique induction with FeCl3 and MgCl2. All the isolates were found to be resistant against cefepime and different clonal patterns of resistance were found among the isolates. Conclusion: This study has drawn a new insight into polymyxin resistance which will help in the detection and control of infections caused by multidrug resistant P. aeruginosa. The low susceptibility rate to aminoglycoside, piperacllin-tazobactam and ciprofloxacin was found and in addition, detection of PmrB and arnA as molecular markers in the follow up of infections caused by multidrug resistant P. aeruginosa.